Microplastics(MPs)have garnered significant international scrutiny as an emerging environmental pollutant,constituting one of the four principal global environmental threats and posing potential health hazards to huma...Microplastics(MPs)have garnered significant international scrutiny as an emerging environmental pollutant,constituting one of the four principal global environmental threats and posing potential health hazards to humans.However,data on the impact of MPs on the early life of the commercially important fish remain limited.In this study,polystyrene microspheres(PS-MPs)(1 and 5μm)were used to investigate the effects of MPs on the growth,development,and metabolism in early life stages of large yellow croaker Pseudosciaena crocea.Results indicate that MPs were enriched in the gastrointestinal tract and gills of the fish.In addition,PS-MPs(1μm)exhibited no obvious effects on embryo hatching and heart rates,while increased the mortality rate(23.00%vs.control 14.99%)and decreased the body length(4098.61±447.03μm vs.control with 2827.04±254.75μm)of the larvae at the highest exposure concentration(5×10^(4)items/L).Metabolomics analysis revealed that PS-MPs(5μm)induced mild perturbations in phospholipid metabolism,specifically alterations in phosphatidylethanolamine(PE)levels.These changes influenced the cell membranes of juvenile fish,and consequently elicited inflammatory responses,disrupted lipid homeostasis,and affected other critical physiological processes.Ultimately,these effects may avoid the growth retardation and potential mortality.Therefore,PS-MPs could affect negatively the fish health in the early life stage,which has implications for aquatic ecosystems.展开更多
Microplastics(MPs)have attracted growing attention worldwide as an increasingly prevalent environmental pollutant.In addition,chicken meat is currently the most widely consumed kind of poultry in the global market.Con...Microplastics(MPs)have attracted growing attention worldwide as an increasingly prevalent environmental pollutant.In addition,chicken meat is currently the most widely consumed kind of poultry in the global market.Consumer demand for chicken is on the rise both at home and abroad.As a result,the safety of chicken raising has also received significant attention.The lungs play an essential role in the physiological activities of chickens,and they are also the most vulnerable organs.Lung injury is difficult to repair after the accumulation of contaminants,and the mortality rate is high,which brings huge economic losses to farmers.The research on the toxicity of MPs has mainly focused on the marine ecosystem,while the mechanisms of toxicity and lung damage in chickens have been poorly studied.Thus,this study explored the effects of exposure to polystyrene microplastics(PS-MPs)at various concentrations for 42 d on chicken lungs.PS-MPs could cause lung pathologies and ultrastructural abnormalities,such as endoplasmic reticulum(ER)swelling,inflammatory cell infiltration,chromatin agglutination,and plasma membrane rupture.Simultaneously,PS-MPs increased the expression of genes related to the heat shock protein family(Hsp60,Hsp70,and Hsp90),ER stress signaling(activating) transcription factor 6(ATF0),ATF4,protein kinase RNA-like ER kinase(PERK),and eukaryotic translation initiation factor 2 subunitα(eIF2a),pyroptosis-related genes(NOD)-,LRR-and pyrin domain-containing protein 3(NLRP3),apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),interleukin-1β(IL-1β),cysteinyl aspartate-specific proteinase 1(Caspasel),and gasdermin-D(GSDMD),and the inflammatory signaling pathway(nuclear factor-kB(NF-kB),inducible nitric oxide synthase(iNOS),and cyclooxygenase-2(COX-2).The above results showed that PS-MP exposure could result in lung stress,ER stress,pyroptosis,and inflammation in broilers.Our findings provide new scientific clues for further research on the mechanisms of physical health and toxicology regardingMPs.展开更多
Microplastics(MPs),which originate from plastic degradation,are becoming a significant environmental pollutant,and their prevalence is increasing rapidly.Humans can ingest MPs through various pathways and their presen...Microplastics(MPs),which originate from plastic degradation,are becoming a significant environmental pollutant,and their prevalence is increasing rapidly.Humans can ingest MPs through various pathways and their presence has been detected in multiple human organs,raising concerns about the potential toxic effects associated with plastic consumption.Epigenetic modifications of nucleic acids play crucial roles in various biological processes,including gene expression and tumorigenesis.Previous studies have demonstrated that exposure to certain environmental pollutants can influence disease pathogenesis by affecting epigenetic factors,including modifications of nucleic acids.However,the impact of MPs on epigenetic modifications of nucleic acids remains largely unexplored.In this study,we systematically investigated the alterations in epigenetic modifications of DNA and RNA following exposure to polystyrene microplastics(PS-MPs).We utilized liquid chromatography-tandem mass spectrometry(LCMS/MS)to simultaneously analyze two DNA epigenetic modifications of 5-methylcytosine(5m C)and 5-hydroxymethylcytosine(5hm C),along with twenty RNA epigenetic modifications from small RNA and nine epigenetic modifications from m RNA.We measured changes in the levels of DNA and RNA modifications across six tissues(heart,liver,spleen,lung,kidney,and intestine)in mice after PS-MPs exposure.The results indicated that exposure to PS-MPs significantly altered the landscape of epigenetic modifications in nucleic acids.Furthermore,we observed tissue-specific effects,suggesting that different organs respond uniquely to PS-MPs exposure.Additionally,the correlation patterns between DNA and RNA modifications changed following PS-MPs exposure.These findings provide valuable insights suggesting that PS-MPs exposure may alter the patterns of epigenetic modifications in nucleic acids,potentially leading to adverse health effects.展开更多
Human embryonic stem cell-originated liver organoids(LOs)were used to evaluate the hepatocytotoxicity of polystyrene microplastics(PS-MPs),with a focus on the cytotoxicity,oxidative stress,and lipid metabolism disrupt...Human embryonic stem cell-originated liver organoids(LOs)were used to evaluate the hepatocytotoxicity of polystyrene microplastics(PS-MPs),with a focus on the cytotoxicity,oxidative stress,and lipid metabolism disruption,and to investigate the protective mechanism of phlorizin(PZ).Results showed that PS-MPs at environmentally relevant concentrations of 0.05,0.25 and 1.25μg/mL induced dose-dependent hep-atocytotoxicity,oxidative stress and lipid accumulation in LOs.Transcriptomics revealed PS-MPs disrupted tri-glyceride homeostasis and antioxidant pathways.Co-treatment with a physiologically attainable dose of PZ(1.4μg/mL)significantly mitigated PS-MPs-induced toxicity,such as reducing apoptosis to control levels,restoring redox balance,and attenuating lipid accumulation.PZ normalized expression of key genes governing fatty acid synthesis,β-oxidation,and lipid droplet formation,while downregulating critical hepatic transcription factors.Immunofluorescence data confirmed PZ reversed PS-MP-induced disruption in CPT1A and SREBF1,primarily by modulating oxidative stress.Despite these improvements,the structural integrity of LOs and ATP levels were not fully restored,suggesting that PS-MPs may cause some persistent damage not fully rescued by PZ.These findings demonstrate PZ alleviates PS-MPs-induced hepatotoxicity by modulating oxidative stress and lipid metabolism pathways,supporting its potential as a protective agent.展开更多
Microplastics have been detected in various foods,revealing potential pathways for their entry into the human body through the food chain.As the primary digestive organ,the gut is also a significant site for microplas...Microplastics have been detected in various foods,revealing potential pathways for their entry into the human body through the food chain.As the primary digestive organ,the gut is also a significant site for microplastic accumulation,yet effective strategies to mitigate gut damage caused by microplastic exposure remain to be explored.This study identified Lactobacillus delbrueckii subsp.bulgaricus strain JP006,which exhibits excellent acid tolerance,cell adhesion properties,and the ability to adsorb polystyrene microplastics(PS-MPs).Using cellular models combined with metabolomics analysis,the primary mechanism by which this strain alleviates PSMP-induced cellular damage was preliminarily revealed.Furthermore,its practical efficacy in mitigating PS-MPinduced intestinal damage was validated in a mouse model.Results indicate that JP006 alleviates PS-MPsinduced oxidative damage by enhancing antioxidant enzyme activity and reducing reactive oxygen species(ROS)levels;metabolomics analysis identified N-acetylglutamate as a key mediating substance.In mouse experiments,JP006 increased fecal PS-MPs excretion from 0.29 to 3.54μg/mL,significantly promoting PS-MPs efflux.16S rRNA amplicon data combined with Real-time Fluorescent Quantitative PCR(qPCR)results showed that JP006 not only restored microbial diversity but also increased the abundance of Lachnospiraceae and JP006 itself in the mouse gut,while reversing PS-MPs-induced overgrowth of Staphylococcus aureus,Enterococcus,and Prevotella.In summary,this study reports for the first time the role of Lactobacillus delbrueckii subsp.bulgaricus in adsorbing microplastics,promoting their intestinal excretion,and regulating intestinal permeability.These findings provide a theoretical basis for further developing this strain as a probiotic formulation to enhance microplastic excretion.展开更多
Microplastics have received increasing attention in soil ecosystems,and their potential impacts on soil properties have raised concerns.Pesticides are the most prevalent pollutants in soil,but their combined effects w...Microplastics have received increasing attention in soil ecosystems,and their potential impacts on soil properties have raised concerns.Pesticides are the most prevalent pollutants in soil,but their combined effects with microplastics on the soil environment have not been elucidated.In this study,polystyrene microplastics(PS MPs)and imidacloprid(IMI)were added to the soil to investigate their combined effects on soil physicochemical characteristics,nitrogen and phosphorus contents,related transformation activities,and the composition of nitrogen-and phosphorus-transforming microorganisms.The results revealed that the coexistence of PS MPs and IMI led to a significantly higher soil pH level and lower water-stable aggregate(WSA)content.Additionally,it increased the relative abundance of nitrogen-and phosphorus-transforming microorganisms,including ammonia-oxidizing archaea and bacteria,nitrite-oxidizing bacteria,heterotrophic denitrifying bacteria,phosphate-solubilizing bacteria.PS MPs increased the soil potential denitrification rate by 14.53%owing to a significantly higher pH level.However,this promotion disappeared when they combined with IMI.The coexistence of PS MPs and IMI caused a significant decrease in WSA content,thereby improving soil aeration and increasing the relative abundance of phosphate-solubilizing bacteria,which led to a 14.54%and 44.79%increase in soil phosphatase activity and Olsen-P content,respectively.Variance partitioning analysis revealed that the coexistence of PS MPs and IMI mainly influenced nitrogen and phosphorus transformations by altering soil pH and WSA content.These results reveal the combined effects of PS MPs and IMI on soil nitrogen and phosphorus transformations and elucidate soil environmental risks associated with microplastics and pesticides.展开更多
Microplastics(MPs)are one of the most concerning pollutants that affects the health and growth of aquatic organisms.We characterized the MPs dispersion in the milli-Q water and seawater,and evaluated the effects of MP...Microplastics(MPs)are one of the most concerning pollutants that affects the health and growth of aquatic organisms.We characterized the MPs dispersion in the milli-Q water and seawater,and evaluated the effects of MPs on the gut epithelial cells of brine shrimp using three sizes of polystyrene(PS)microbeads(0.05,0.5,and 5μm,respectively).Results show that microbeads evenly dispersed in milli-Q water,but exhibited aggregation tendency in seawater associating with the particle size.Apart from a reduced survival rate,we observed the structure changes in the gut epithelium that the smaller size of PS microbeads resulted in an increased reactive oxygen species(ROS)and higher apoptosis-related genes expression.Moreover,exposure to all size of PS microbeads led to increased green fluorescence of J-monomer,indicating the declined mitochondrial membrane potential.Therefore,exposure to PS microbeads led to significantly size-dependent toxicity on brine shrimp.Especially,0.05-μm PS microbeads were more toxic,leading to severe oxidative stress and activation of the p53-Bax-Bcl2 pathway,ultimately resulting in cellular apoptosis and gut damage.These findings are important to understand the mechanism of MPs toxicity and its potential ecological risks to marine aquatic animals.展开更多
Microplastics(MPs)are considered one of the main causes of male and female infertility.However,the reproductive toxicity and its related mechanisms are currently understood primarily through animal models with acute e...Microplastics(MPs)are considered one of the main causes of male and female infertility.However,the reproductive toxicity and its related mechanisms are currently understood primarily through animal models with acute exposure to MPs.In this study,we demonstrate that lowdose exposure to polystyrene microplastics(PSMPs)leads to severely abnormal reproduction in females,manifested by oocyte meiotic maturation defect.Mechanistically,PSMPs exposure induce the overactivation of cell metabolism pathways,insufficient HDACs,and H4K16 hyperacetylation in oocytes both in vivo and in vitro.When an HDAC3 inhibitor is added,the oocyte maturation defect,overactivation of cell metabolism pathways,and H4K16 hyperacetylation are recapitulated.Conversely,the overexpression of HDAC3 can rescue the defects in meiotic maturation induced by PSMPs.Our observations suggest a direct link between the maturation defects caused by PSMPs and HDAC3 insufficiency.Thus,we propose potential treatments to address the meiotic maturation defect of oocytes in women highly exposed to MPs by activating or supplying HDAC3.展开更多
基金Supported by the Pioneer and Leading Goose R&D Program of Zhejiang(No.2023C03130)the National Key R&D Program of China(No.2019YFD0901101)+4 种基金the National Natural Science Foundation of China(No.42076169)the Oceanic Interdisciplinary Program of Shanghai Jiao Tong University(Nos.SL2022ZD203,SL2022MS012)the Zhejiang Provincial Natural Science Founds for Distinguished Young Scientists(No.LR21D060001)the State Key Laboratory of Satellite Ocean Environment Dynamics(No.SOEDZZ1902)the ChinaAPEC Cooperation Fund(No.2029901)。
文摘Microplastics(MPs)have garnered significant international scrutiny as an emerging environmental pollutant,constituting one of the four principal global environmental threats and posing potential health hazards to humans.However,data on the impact of MPs on the early life of the commercially important fish remain limited.In this study,polystyrene microspheres(PS-MPs)(1 and 5μm)were used to investigate the effects of MPs on the growth,development,and metabolism in early life stages of large yellow croaker Pseudosciaena crocea.Results indicate that MPs were enriched in the gastrointestinal tract and gills of the fish.In addition,PS-MPs(1μm)exhibited no obvious effects on embryo hatching and heart rates,while increased the mortality rate(23.00%vs.control 14.99%)and decreased the body length(4098.61±447.03μm vs.control with 2827.04±254.75μm)of the larvae at the highest exposure concentration(5×10^(4)items/L).Metabolomics analysis revealed that PS-MPs(5μm)induced mild perturbations in phospholipid metabolism,specifically alterations in phosphatidylethanolamine(PE)levels.These changes influenced the cell membranes of juvenile fish,and consequently elicited inflammatory responses,disrupted lipid homeostasis,and affected other critical physiological processes.Ultimately,these effects may avoid the growth retardation and potential mortality.Therefore,PS-MPs could affect negatively the fish health in the early life stage,which has implications for aquatic ecosystems.
基金This work was supported by the Key Projects of Natural Science Foundation of Heilongjiang Province of China(No.ZD2020C005).
文摘Microplastics(MPs)have attracted growing attention worldwide as an increasingly prevalent environmental pollutant.In addition,chicken meat is currently the most widely consumed kind of poultry in the global market.Consumer demand for chicken is on the rise both at home and abroad.As a result,the safety of chicken raising has also received significant attention.The lungs play an essential role in the physiological activities of chickens,and they are also the most vulnerable organs.Lung injury is difficult to repair after the accumulation of contaminants,and the mortality rate is high,which brings huge economic losses to farmers.The research on the toxicity of MPs has mainly focused on the marine ecosystem,while the mechanisms of toxicity and lung damage in chickens have been poorly studied.Thus,this study explored the effects of exposure to polystyrene microplastics(PS-MPs)at various concentrations for 42 d on chicken lungs.PS-MPs could cause lung pathologies and ultrastructural abnormalities,such as endoplasmic reticulum(ER)swelling,inflammatory cell infiltration,chromatin agglutination,and plasma membrane rupture.Simultaneously,PS-MPs increased the expression of genes related to the heat shock protein family(Hsp60,Hsp70,and Hsp90),ER stress signaling(activating) transcription factor 6(ATF0),ATF4,protein kinase RNA-like ER kinase(PERK),and eukaryotic translation initiation factor 2 subunitα(eIF2a),pyroptosis-related genes(NOD)-,LRR-and pyrin domain-containing protein 3(NLRP3),apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),interleukin-1β(IL-1β),cysteinyl aspartate-specific proteinase 1(Caspasel),and gasdermin-D(GSDMD),and the inflammatory signaling pathway(nuclear factor-kB(NF-kB),inducible nitric oxide synthase(iNOS),and cyclooxygenase-2(COX-2).The above results showed that PS-MP exposure could result in lung stress,ER stress,pyroptosis,and inflammation in broilers.Our findings provide new scientific clues for further research on the mechanisms of physical health and toxicology regardingMPs.
基金supported by the National Key R&D Program of China(Nos.2022YFA0806600,2022YFC3400700)the National Natural Science Foundation of China(No.22277093)the Key Research and Development Project of Hubei Province(No.2023BCB094)。
文摘Microplastics(MPs),which originate from plastic degradation,are becoming a significant environmental pollutant,and their prevalence is increasing rapidly.Humans can ingest MPs through various pathways and their presence has been detected in multiple human organs,raising concerns about the potential toxic effects associated with plastic consumption.Epigenetic modifications of nucleic acids play crucial roles in various biological processes,including gene expression and tumorigenesis.Previous studies have demonstrated that exposure to certain environmental pollutants can influence disease pathogenesis by affecting epigenetic factors,including modifications of nucleic acids.However,the impact of MPs on epigenetic modifications of nucleic acids remains largely unexplored.In this study,we systematically investigated the alterations in epigenetic modifications of DNA and RNA following exposure to polystyrene microplastics(PS-MPs).We utilized liquid chromatography-tandem mass spectrometry(LCMS/MS)to simultaneously analyze two DNA epigenetic modifications of 5-methylcytosine(5m C)and 5-hydroxymethylcytosine(5hm C),along with twenty RNA epigenetic modifications from small RNA and nine epigenetic modifications from m RNA.We measured changes in the levels of DNA and RNA modifications across six tissues(heart,liver,spleen,lung,kidney,and intestine)in mice after PS-MPs exposure.The results indicated that exposure to PS-MPs significantly altered the landscape of epigenetic modifications in nucleic acids.Furthermore,we observed tissue-specific effects,suggesting that different organs respond uniquely to PS-MPs exposure.Additionally,the correlation patterns between DNA and RNA modifications changed following PS-MPs exposure.These findings provide valuable insights suggesting that PS-MPs exposure may alter the patterns of epigenetic modifications in nucleic acids,potentially leading to adverse health effects.
基金supported by the“National Key R&D Program of China(2024YFD2402204)”.
文摘Human embryonic stem cell-originated liver organoids(LOs)were used to evaluate the hepatocytotoxicity of polystyrene microplastics(PS-MPs),with a focus on the cytotoxicity,oxidative stress,and lipid metabolism disruption,and to investigate the protective mechanism of phlorizin(PZ).Results showed that PS-MPs at environmentally relevant concentrations of 0.05,0.25 and 1.25μg/mL induced dose-dependent hep-atocytotoxicity,oxidative stress and lipid accumulation in LOs.Transcriptomics revealed PS-MPs disrupted tri-glyceride homeostasis and antioxidant pathways.Co-treatment with a physiologically attainable dose of PZ(1.4μg/mL)significantly mitigated PS-MPs-induced toxicity,such as reducing apoptosis to control levels,restoring redox balance,and attenuating lipid accumulation.PZ normalized expression of key genes governing fatty acid synthesis,β-oxidation,and lipid droplet formation,while downregulating critical hepatic transcription factors.Immunofluorescence data confirmed PZ reversed PS-MP-induced disruption in CPT1A and SREBF1,primarily by modulating oxidative stress.Despite these improvements,the structural integrity of LOs and ATP levels were not fully restored,suggesting that PS-MPs may cause some persistent damage not fully rescued by PZ.These findings demonstrate PZ alleviates PS-MPs-induced hepatotoxicity by modulating oxidative stress and lipid metabolism pathways,supporting its potential as a protective agent.
基金supported by the Jiangsu Basic Research Center for Synthetic Biology Grant(No.BK20233003).
文摘Microplastics have been detected in various foods,revealing potential pathways for their entry into the human body through the food chain.As the primary digestive organ,the gut is also a significant site for microplastic accumulation,yet effective strategies to mitigate gut damage caused by microplastic exposure remain to be explored.This study identified Lactobacillus delbrueckii subsp.bulgaricus strain JP006,which exhibits excellent acid tolerance,cell adhesion properties,and the ability to adsorb polystyrene microplastics(PS-MPs).Using cellular models combined with metabolomics analysis,the primary mechanism by which this strain alleviates PSMP-induced cellular damage was preliminarily revealed.Furthermore,its practical efficacy in mitigating PS-MPinduced intestinal damage was validated in a mouse model.Results indicate that JP006 alleviates PS-MPsinduced oxidative damage by enhancing antioxidant enzyme activity and reducing reactive oxygen species(ROS)levels;metabolomics analysis identified N-acetylglutamate as a key mediating substance.In mouse experiments,JP006 increased fecal PS-MPs excretion from 0.29 to 3.54μg/mL,significantly promoting PS-MPs efflux.16S rRNA amplicon data combined with Real-time Fluorescent Quantitative PCR(qPCR)results showed that JP006 not only restored microbial diversity but also increased the abundance of Lachnospiraceae and JP006 itself in the mouse gut,while reversing PS-MPs-induced overgrowth of Staphylococcus aureus,Enterococcus,and Prevotella.In summary,this study reports for the first time the role of Lactobacillus delbrueckii subsp.bulgaricus in adsorbing microplastics,promoting their intestinal excretion,and regulating intestinal permeability.These findings provide a theoretical basis for further developing this strain as a probiotic formulation to enhance microplastic excretion.
基金supported by the National Natural Science Foundation of China(Nos.32001195 and 42207022)the Joint Funds of the National Natural Science Foundation of China(No.U2340219)National Funding for Postdoctoral Researchers Program(China)(No.GZC20233319).
文摘Microplastics have received increasing attention in soil ecosystems,and their potential impacts on soil properties have raised concerns.Pesticides are the most prevalent pollutants in soil,but their combined effects with microplastics on the soil environment have not been elucidated.In this study,polystyrene microplastics(PS MPs)and imidacloprid(IMI)were added to the soil to investigate their combined effects on soil physicochemical characteristics,nitrogen and phosphorus contents,related transformation activities,and the composition of nitrogen-and phosphorus-transforming microorganisms.The results revealed that the coexistence of PS MPs and IMI led to a significantly higher soil pH level and lower water-stable aggregate(WSA)content.Additionally,it increased the relative abundance of nitrogen-and phosphorus-transforming microorganisms,including ammonia-oxidizing archaea and bacteria,nitrite-oxidizing bacteria,heterotrophic denitrifying bacteria,phosphate-solubilizing bacteria.PS MPs increased the soil potential denitrification rate by 14.53%owing to a significantly higher pH level.However,this promotion disappeared when they combined with IMI.The coexistence of PS MPs and IMI caused a significant decrease in WSA content,thereby improving soil aeration and increasing the relative abundance of phosphate-solubilizing bacteria,which led to a 14.54%and 44.79%increase in soil phosphatase activity and Olsen-P content,respectively.Variance partitioning analysis revealed that the coexistence of PS MPs and IMI mainly influenced nitrogen and phosphorus transformations by altering soil pH and WSA content.These results reveal the combined effects of PS MPs and IMI on soil nitrogen and phosphorus transformations and elucidate soil environmental risks associated with microplastics and pesticides.
基金Supported by the Program of Sustainable Development and Protection of Artemia Resources in Yuncheng Salt Lake of China(No.YHYJ-2023005)。
文摘Microplastics(MPs)are one of the most concerning pollutants that affects the health and growth of aquatic organisms.We characterized the MPs dispersion in the milli-Q water and seawater,and evaluated the effects of MPs on the gut epithelial cells of brine shrimp using three sizes of polystyrene(PS)microbeads(0.05,0.5,and 5μm,respectively).Results show that microbeads evenly dispersed in milli-Q water,but exhibited aggregation tendency in seawater associating with the particle size.Apart from a reduced survival rate,we observed the structure changes in the gut epithelium that the smaller size of PS microbeads resulted in an increased reactive oxygen species(ROS)and higher apoptosis-related genes expression.Moreover,exposure to all size of PS microbeads led to increased green fluorescence of J-monomer,indicating the declined mitochondrial membrane potential.Therefore,exposure to PS microbeads led to significantly size-dependent toxicity on brine shrimp.Especially,0.05-μm PS microbeads were more toxic,leading to severe oxidative stress and activation of the p53-Bax-Bcl2 pathway,ultimately resulting in cellular apoptosis and gut damage.These findings are important to understand the mechanism of MPs toxicity and its potential ecological risks to marine aquatic animals.
基金supported by the National Natural Science Foundation of China(32370862)Basic Public Welfare Research Program of Zhejiang Province(LY22C120001)Natural Science Foundation of Zhejiang Province(LQ24C120002).
文摘Microplastics(MPs)are considered one of the main causes of male and female infertility.However,the reproductive toxicity and its related mechanisms are currently understood primarily through animal models with acute exposure to MPs.In this study,we demonstrate that lowdose exposure to polystyrene microplastics(PSMPs)leads to severely abnormal reproduction in females,manifested by oocyte meiotic maturation defect.Mechanistically,PSMPs exposure induce the overactivation of cell metabolism pathways,insufficient HDACs,and H4K16 hyperacetylation in oocytes both in vivo and in vitro.When an HDAC3 inhibitor is added,the oocyte maturation defect,overactivation of cell metabolism pathways,and H4K16 hyperacetylation are recapitulated.Conversely,the overexpression of HDAC3 can rescue the defects in meiotic maturation induced by PSMPs.Our observations suggest a direct link between the maturation defects caused by PSMPs and HDAC3 insufficiency.Thus,we propose potential treatments to address the meiotic maturation defect of oocytes in women highly exposed to MPs by activating or supplying HDAC3.
