玉米南方锈病在我国海南、云南、广西等玉米种植区发生且逐渐北移,达到黄淮、华北、东北乃至西北玉米种植区。在全球范围内,玉米南方锈病正在蔓延,且逐年加重。玉米南方锈病研究发展现状和研究前沿亟待梳理。本研究整理并筛选了历来CNK...玉米南方锈病在我国海南、云南、广西等玉米种植区发生且逐渐北移,达到黄淮、华北、东北乃至西北玉米种植区。在全球范围内,玉米南方锈病正在蔓延,且逐年加重。玉米南方锈病研究发展现状和研究前沿亟待梳理。本研究整理并筛选了历来CNKI数据库和Web of Science核心合集收录的玉米南方锈病相关文献,并应用CiteSpace分析软件对相关文献进行可视化分析。结果显示:在作者合作方面,玉米南方锈病研究已经形成相对稳定的团队,但团队间的交流有待加强;在机构合作方面,头部机构间共现较少,机构间的合作生态有待加强;在研究热点方面,中文期刊聚焦于“玉米抗病性”、“流行因素”、“鲜食玉米”及“防治措施”等关键词,化学防治成为当前的研究凸现热点,国内期刊的广度优于国际期刊,国际期刊则更关注遗传(Inheritance)、抗病性(disease resistance)、玉米(zea may)和鉴定(identification)等关键词,“gene”、“identification”、“immunity”等成为当前的研究凸现热点,国际期刊深度优于国内期刊;总之,无论是中文期刊还是国际期刊,抗病性研究都是玉米南方锈病领域的核心议题,但中文期刊更侧重于宏观研究,而国际期刊则更早地转向了分子和基因层面的研究,并持续在这一领域保持研究热度。展开更多
Southern rust （Puccinia polysora Underw.） is a major disease that can cause severe yield losses in maize （Zea mays L.）. In our previous study, a major gene RppP25 that confers resistance to southern rust was ident...Southern rust （Puccinia polysora Underw.） is a major disease that can cause severe yield losses in maize （Zea mays L.）. In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 （as the recurrent parent）. The 1：1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat （SSR） markers and seven insertion/deletion （InDel） markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat （NBS-LRR） protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.展开更多
文摘玉米南方锈病在我国海南、云南、广西等玉米种植区发生且逐渐北移,达到黄淮、华北、东北乃至西北玉米种植区。在全球范围内,玉米南方锈病正在蔓延,且逐年加重。玉米南方锈病研究发展现状和研究前沿亟待梳理。本研究整理并筛选了历来CNKI数据库和Web of Science核心合集收录的玉米南方锈病相关文献,并应用CiteSpace分析软件对相关文献进行可视化分析。结果显示:在作者合作方面,玉米南方锈病研究已经形成相对稳定的团队,但团队间的交流有待加强;在机构合作方面,头部机构间共现较少,机构间的合作生态有待加强;在研究热点方面,中文期刊聚焦于“玉米抗病性”、“流行因素”、“鲜食玉米”及“防治措施”等关键词,化学防治成为当前的研究凸现热点,国内期刊的广度优于国际期刊,国际期刊则更关注遗传(Inheritance)、抗病性(disease resistance)、玉米(zea may)和鉴定(identification)等关键词,“gene”、“identification”、“immunity”等成为当前的研究凸现热点,国际期刊深度优于国内期刊;总之,无论是中文期刊还是国际期刊,抗病性研究都是玉米南方锈病领域的核心议题,但中文期刊更侧重于宏观研究,而国际期刊则更早地转向了分子和基因层面的研究,并持续在这一领域保持研究热度。
基金supported by the State Key Basic Research and Development Plan of China(973)(2009CB118400)the National Hi-Tech Research and Development Program of China(863)(2011AA10A103)
文摘Southern rust （Puccinia polysora Underw.） is a major disease that can cause severe yield losses in maize （Zea mays L.）. In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 （as the recurrent parent）. The 1：1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat （SSR） markers and seven insertion/deletion （InDel） markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat （NBS-LRR） protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.
