Polyphenols,a diverse group of naturally occurring compounds found in plants,have garnered significant attention for their potential therapeutic properties in treating neurodegenerative diseases(NDs).The Wnt/β-cateni...Polyphenols,a diverse group of naturally occurring compounds found in plants,have garnered significant attention for their potential therapeutic properties in treating neurodegenerative diseases(NDs).The Wnt/β-catenin(WβC)signaling pathway,a crucial player in neurogenesis,neuronal survival,and synaptic plasticity,is involved in several cellular mechanisms related to NDs.Dysregulation of this pathway is a hallmark in the development of various NDs.This study explores multiple polyphenolic compounds,such as flavonoids,stilbenes,lignans,and phenolic acids,and their potential to protect the nervous system.It provides a comprehensive analysis of their effects on the WβC pathway,elucidating their modes of action.The study highlights the dual function of polyphenols in regulating and protecting the nervous system,providing reassurance about the research benefits.This review provides a comprehensive analysis of the results obtained from both in vitro studies and in vivo research,shedding light on how these substances influence the various components of the pathway.The focus is mainly on the molecular mechanisms that allow polyphenols to reduce oxidative stress,inflammation,and apoptotic processes,ultimately improving the function and survival of neurons.This study aims to offer a thorough understanding of the potential of polyphenols in targeting the WβC signaling pathway,which could lead to the development of innovative therapeutic options for NDs.展开更多
High-entropy alloy(HEA)nanoparticles(NPs)have attracted great attention in electrocatalysis due to their tailorable complex compositions and unique properties.Herein,we introduce Fe,Co,Ni,Cr and Mn into the metal-poly...High-entropy alloy(HEA)nanoparticles(NPs)have attracted great attention in electrocatalysis due to their tailorable complex compositions and unique properties.Herein,we introduce Fe,Co,Ni,Cr and Mn into the metal-polyphenol coordination system to prepare HEA NPs enclosed in N-doped carbon(FeCoNiCrMn)with great potential for catalyzing oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).The unique high-entropy structural characteristics in FeCoNiCrMn facilitate effective interplay between metal species,leading to improved ORR(E_(1/2)=0.89 V)and OER(η=330 mV,j=10 mA·cm^(−2))activity.Additionally,FeCoNiCrMn exhibits excellent open-circuit voltage(1.523 V),power density(110 mW·cm^(−2))and long-term durability,outperforming Pt/C+IrO_(2) electrodes as a cathode catalyst in Zn-air batteries(ZABs).Such polyphenol-assisted alloying method broadens and simplifies the development of HEA electrocatalysts for high-performance ZABs.展开更多
Acetaminophen(APAP)used as an antipyretic and analgesic agent can cause acute liver injury(AILI)under overdose.Sanghuangporous vaninii is an edible fungus with abundant metabolites exhibits excellent hepatoprotective ...Acetaminophen(APAP)used as an antipyretic and analgesic agent can cause acute liver injury(AILI)under overdose.Sanghuangporous vaninii is an edible fungus with abundant metabolites exhibits excellent hepatoprotective activities,but the effect for AILI is not yet fully understood.In this study,the polyphenol rich extract from S.vaninii(PSV)was prepared,with a total phenolic content of 75.72%and 34 compounds.The data of hepatoprotection indicated that PSV obviously alleviated the hepatocellular injury induced by APAP in vivo and in vitro.The protective mechanism of PSV against APAP-induced AILI might be attributed to the activating NRF2/GPX4 pathway.Based on network pharmacology analysis,the active components of PSV such as caffeic acid,osmundacetone and hispolone played a key role in hepatoprotection of PSV.Consequently,this study highlights the protection of PSV on AILI,which provides new insight into bioactivities of S.vaninii.展开更多
Quinoa is pseudo cereal and considered as full nutritional food for its functional ingredients such as peptides,polysaccharides,saponins and polyphenols.Up to now,over 20 phenolic compounds have been reported in quino...Quinoa is pseudo cereal and considered as full nutritional food for its functional ingredients such as peptides,polysaccharides,saponins and polyphenols.Up to now,over 20 phenolic compounds have been reported in quinoa,and these polyphenols are related to anti-cancer,anti-inflammatory,anti-obesity,anti-diabetic and cardioprotective activities.Recently,more and more attentions are focused on quinoa in the food and pharmaceutical fields.Many new products such as bakery,beverage and meat product made from quinoa are popular in the market.This article presents a review of the literature on the function and application of polyphenols in quinoa.The review will benefit the researchers working with nutrition,functional diets of quinoa.展开更多
Epilepsy is a devastating neurological condition that affects over 70 million people worldwide.Apart from idiopathic epilepsy,numerous diseases have been reported to cause secondary epilepsy.Polyphenols,ubiquitously e...Epilepsy is a devastating neurological condition that affects over 70 million people worldwide.Apart from idiopathic epilepsy,numerous diseases have been reported to cause secondary epilepsy.Polyphenols,ubiquitously existing in fruits and vegetables,has become a promising neuroprotective candidate against multiple neurological abnormalities due to its interplay with gut microbiome composed of the bacterial population in gastrointestinal system.This bioactive compound is also being tested for the management of epileptogenesis.However,there is still insufficient evidence from mechanistic studies to demonstrate the causal relationship between anti-epileptic effect of polyphenols and microbial alteration.In this review,we provide the brief overview of epilepsy,polyphenols and gut microbiota.We then analysis the association of polyphenols and gut microbiota particularly in the epilepsy and symptomatic seizures-induced by epilepsy related disorders.We also discuss the possibility for the combined application with probiotics to improve the bioavailability and bioaccessibility of polyphenols for expanding its anti-epileptic function.We finally summarize the therapeutic potential of anti-seizure polyphenols through affecting gut microbiome,and propose that the anti-epileptic effect of polyphenols may be tightly relevant to intestinal bacterial flora for overcoming epilepsy and its comorbidities simultaneously.Therefore,the dietary polyphenols targeting specific bacteria may achieve the outcome of‘one stone,two birds’.展开更多
Depression is a common psychiatric condition with a considerable influence on global health.Although current pharmacotherapeutic choices are of indisputable relevance,there is sustained interest in natural medicines f...Depression is a common psychiatric condition with a considerable influence on global health.Although current pharmacotherapeutic choices are of indisputable relevance,there is sustained interest in natural medicines for treating depressive symptoms.Polyphenols,a class of plant-derived natural compounds,have received attention due to their possible neuroprotective and anti-depressive properties.In this review,we aimed to present the intricate mechanisms through which polyphenols modulate inflammation,neurogenesis,oxidative stress,and the gut microbiota.Consumption of polyphenol-rich diets is emerging as a non-invasive,natural,and cost-effective approach to supporting brain health,with potential influence on depressive symptoms.While there have been promising results,more research is needed to draw solid conclusions regarding the medicinal potential of polyphenols.展开更多
The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking.The TCMSP pharmacology database was used ...The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking.The TCMSP pharmacology database was used to screen the polyphenols present in sea buckthorn,and then the SwissTargetPrediction and Uniprot databases were used to obtain the potential targets of sea buckthorn polyphenols,which were supplemented by the literature.In total,7 polyphenols and 154 potential targets were obtained.Through GeneCards,OMIM database,1358 hyperlipidemia-related targets were collected.We found that there were 101 targets at the intersection of components and diseases.Through GO and KEGG enrichment analysis,27 core targets were obtained,which were AKT1,TNF,TP53,IL-6,etc.in order of degree value.174 pathways were obtained from KEGG enrichment analysis,including AGE-RAGE signaling pathway in diabetic complications,fl uid shear stress and atherosclerosis,lipid and atherosclerosis,etc.The molecular docking of the main components to the targets was performed using OpenBabelGUI,AutoDockTools-1.5.6 software.Finally,the results were visualized using Cytoscape 3.9.1 software.The molecular docking results showed that sea buckthorn polyphenols have good binding ability with the key targets.Among them,such as quercetin and kaempferol,have good binding ability with TNF,TP53 and IL-6.For example,TNF binds to quercetin with a binding energy of-5.34 kcal/mol and to kaempferol with a binding energy of-6.22 kcal/mol;TP53 binds to kaempferol with a binding energy of-5.32 kcal/mol;IL-6 binds to quercetin with a binding energy of-5.62 kcal/mol,etc.Therefore,the network pharmacology study showed that the treatment of hyperlipidemia by sea buckthorn polyphenols can be realized by multi-component-multi-target-multi-pathway together,which provides some reference for the later study of sea buckthorn polyphenols in the treatment of hyperlipidemia.展开更多
The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint materials.However,the pursuit of sustained antibacter...The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint materials.However,the pursuit of sustained antibacterial efficacy remains a formidable challenge.In this context,we proposed a novel strategy that leverages swelling and erosion mechanisms to facilitate drug release of drug-loaded ultrahigh molecular weight polyethylene(UHMWPE),thereby ensuring its long-lasting antibacterial performance.Polyethylene oxide(PEO),a hydrophilic polymer with fast hydrating ability and high swelling capacity,was incorporated in UHMWPE alongside the antibacterial tea polyphenol(epigallocatechin gallate,EGCG as representative).The swelling of PEO enhanced water infiltration into the matrix,while the erosion of PEO balanced the release of the encapsulated EGCG,resulting in a steady release.The behavior was supported by the EGCG release profiles and the corresponding fitted release kinetic models.As demonstrated by segmented antibacterial assessments,the antibacterial efficiency was enhanced 2to 3 times in the PEO/EGCG/UHMWPE composite compared to that of EGCG/UHMWPE.Additionally,the PEO/EGCG/UHMWPE composite exhibited favorable biocompatibility and mechanical performance,making it a potential candidate for the development of drug-releasing joint implants to combat prosthetic bacterial infections.展开更多
The absence of trace amounts of natural bioactive compounds with important biological activities in traditional dietary models for global farm animals,coupled with an incomplete theoretical system for animal nutrition...The absence of trace amounts of natural bioactive compounds with important biological activities in traditional dietary models for global farm animals,coupled with an incomplete theoretical system for animal nutrition,has led to unbalanced and inadequate animal nutrition.This deficiency has adversely impacted animal health and the ecological environment,presenting formidable challenges to the advancement of the swine breeding industry in various countries around the world toward high-quality development.Recently,due to the ban of antibiotics for growth promotion in swine diets,botanical active compounds have been extensively investigated as feed additives.Polyphenols represent a broad group of plant secondary metabolites.They are natural,non-toxic,pollution-free,and highly reproducible compounds that have a wide range of physiological functions,such as antioxidant,anti-inflammatory,immunomodulatory,antiviral,antibacterial,and metabolic activities.Accordingly,polyphenols have been widely studied and used as feed additives in swine production.This review summarizes the structural characteristics,classification,current application situation,general properties of polyphenols,and the latest research advances on their use in swine production.Additionally,the research and application bottlenecks and future development of plant polyphenols in the animal feed industry are reviewed and prospected.This review aims to stimulate the in-depth study of natural plant polyphenols and the research and development of related products in order to promote the green,healthy,and high-quality development of swine production,while also providing ideas for the innovation and development in the theoretical system of animal nutrition.展开更多
Apple polyphenols are widely present in apple pulp,peel,kernel,and leaves.They are natural bioactive substances with a variety of health functions.This review describes the antioxidant functions of anthocyanins,querce...Apple polyphenols are widely present in apple pulp,peel,kernel,and leaves.They are natural bioactive substances with a variety of health functions.This review describes the antioxidant functions of anthocyanins,quercetin,phlorizin,chlorogenic acid,and epicatechin in apple polyphenols through the regulation of signal pathways and transcription factors,and the inhibition or induction of enzymes.Massive food applications of apple polyphenols are summarized,including excellent color protection of fruits and vegetables,meat preservation,preparation of composite packaging fi lms as active ingredients,enhancement of nutritional value as functional ingredients,and improvement of food taste and physical stability.This review would provide a reference for the exploration of apple polyphenol resources and its development in the food field.展开更多
Emerging evidence highlights the potential of bioactive compounds,particularly polyphenols,as adjunctive therapeutic agents in the treatment of pancreatic cancer(PC),one of the most aggressive malignancies.This review...Emerging evidence highlights the potential of bioactive compounds,particularly polyphenols,as adjunctive therapeutic agents in the treatment of pancreatic cancer(PC),one of the most aggressive malignancies.This review focuses on epigallocatechin gallate(EGCG)and resveratrol due to their extensively documented anticancer activity,favorable safety profiles,and their unique ability to modulate multiple signaling pathways relevant to pan-creatic tumorigenesis.Among polyphenols,these two have shown superior anti-cancer activity,epigenetic regulatory effects,and synergy with standard chemotherapies in preclinical pancreatic cancer models.Resveratrol exhibits anti-proliferative effects by modulating key signaling pathways,including phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt),nuclear factor kappa-B(NF-κB),and tumor protein 53(p53).EGCG exerts anti-cancer activity by targeting multiple cellular processes,such as oxidative stress reduction,and suppression of inflammatory mediators like Interleukin-6(IL-6)and Tumor Necrosis Factor-α(TNF-α).Both EGCG and resveratrol exert anti-pancreatic cancer effects partly through direct interactions with cell surface receptors and modulation of intracellular cascades.EGCG targets the 67 kDa laminin receptor(67LR),which is overexpressed in pancreatic cancer cells,triggering apoptosis,cyclic guanosine monophosphate(cGMP)production and activation of the PKCδ/acid sphingomyelinase(ASM)cascade.Resveratrol inhibits insulin-like growth factor-1 receptor(IGF-1R)activation of the PI3K/Akt and Wnt signaling pathways,while concurrently activating tumor suppressor p53.These interactions suppress proliferation,promote apoptosis,and reduce epithelial-mesenchymal transition(EMT),thereby limiting tumor progression.Both polyphenols enhance chemosensitivity and reduce resistance to conventional therapies,including gemcitabine,by modulating drug transporters and apoptotic pathways.Furthermore,their epigenetic influence,particularly via DNA methylation and histone modification,suggests a broader role in pancreatic cancer prevention.Understanding the roles and mechanisms of resveratrol and EGCG in pancreatic cancer provides valuable insights into novel treatment strategies.The integration of polyphenols into conventional therapeutic approaches may offer new hope for improving patient outcomes.展开更多
Gut microbiota regulate the activation of adipose browning,which promote energy dissipation and combat diet-induced obesity.Pomegranate peel polyphenols(PPPs)have been shown to reduce obesity,regulate lipid metabolism...Gut microbiota regulate the activation of adipose browning,which promote energy dissipation and combat diet-induced obesity.Pomegranate peel polyphenols(PPPs)have been shown to reduce obesity,regulate lipid metabolism in adipose tissue,and modulate the composition of gut microbiota in animal fed high-fat diet(HFD).However,the role of gut microbiota in the improvement of obesity by PPPs has not been elucidated.In current study,we applied antibiotics to inhibit gut microbiota in mice fed HFD and treated with PPPs.The results showed that the inhibition of gut microbiota impair the effect of PPPs on reducing obesity and promoting adipose browning,and change the fecal metabolomic profiles in respond to PPPs.Moreover,the inhibition of gut microbiota supressed the promotive effects of PPPs on the levels of Akkermansia and microbiota-related metabolites,such as urolithin A,short-chain fatty acids(SCFAs),and bile acids(BAs),which were associated with activating adipose browning.Therefore,our results suggested that the presence of gut microbiota is essential for PPPs to ameliorate HFD-induced obesity.The related bacteria or metabolites generated by the interaction between PPPs and microbiota promote adipose browning and facilitate the beneficial effects of PPPs.展开更多
This study employs combined network pharmacology and molecular docking approaches to investigate the potential mechanisms by which Erigeron breviscapus polyphenols inhibit liver fibrosis.Active compounds were identifi...This study employs combined network pharmacology and molecular docking approaches to investigate the potential mechanisms by which Erigeron breviscapus polyphenols inhibit liver fibrosis.Active compounds were identified through literature mining,with targets predicted using TCMSP,PubChem,SwissTarget,and SwissADME databases.Liver fibrosis-related targets were retrieved from GeneCards,OMIM,and TTD.Following rigorous screening,12 bioactive polyphenolic compounds and 117 corresponding targets were identified,intersecting with 8,375 liver fibrosis targets to yield 67 common targets.Protein-protein interaction analysis revealed 80 key targets(e.g.,EGFR,ESR1,PTGS2).GO and KEGG analyses indicated enrichment in 352 biological terms and 50 pathways,including chemical carcinogenesis receptor activation and steroid hormone biosynthesis.Molecular docking confirmed effective binding affinity between the top four compounds(by degree value)and their respective targets.In summary,the results of this study indicate that Erigeron breviscapus can inhibit the development of liver fibrosis and related diseases through multiple components,targets,and pathways.This study provides a solid theoretical basis for the research of Erigeron breviscapus in the field of anti liver fibrosis.展开更多
During obesity treatment,clinicians have been burdened by the ever-increasing number of patients and the side effects of drug treatment.Finding new plant-derived active ingredients as an intervention for obesity has b...During obesity treatment,clinicians have been burdened by the ever-increasing number of patients and the side effects of drug treatment.Finding new plant-derived active ingredients as an intervention for obesity has become a research focus.In this study,Plackett-Burman and Central Composite Design experiments,plant-wide target metabolomics technology,simulated human colon fermentation test,and cell experiment in vitro were successively used to investigate the effects of fermented and unfermented polyphenols(FP and UP,respectively)of blue honeysuckle on obesity.After the optimization of the response surface method,polyphenol content((1878.08±14.53)mg/100 g)increased by 2.63 times after Lactobacillus rhamnosus 6224 and Saccharomyces cerevisiae W5 mixed fermentation.There were 23 significantly different metabolites were observed in FP and UP.Of them,7 different metabolites were significantly and positively correlated with short-chain fatty acids(SCFAs),whereas negatively correlated with lipopolysaccharides(LPS).The contents of these 7 metabolites were significantly increased in FP.Furthermore,the effects of FP and UP on the SCFA biosynthesis pathway were different.Cell experiments revealed that polyphenols can significantly promote the secretion of gastrointestinal endogenous hormones and insulin and inhibit interleukin-6 levels.Linear regression analysis revealed SCFAs had a positive regulatory effect on glycolipid metabolism,whereas LPS had a negative effect.Overall,these crucial findings provide an economical and simple method for obtaining blue honeysuckle polyphenols and laying a theoretical foundation for its application in the therapeutic intervention against obesity.展开更多
Climate change is significantly altering viticultural practices worldwide,with profound implications for the accumulation of polyphenolic compounds that determine wine’s sensory and health properties.This review summ...Climate change is significantly altering viticultural practices worldwide,with profound implications for the accumulation of polyphenolic compounds that determine wine’s sensory and health properties.This review summarizes the effects of climate change,particularly rising temperatures,shifting precipitation patterns,and altered light conditions-on polyphenol synthesis in Vitis amurensis(V.amurensis)grapes from Northeast China,the country’s highest-latitude wine region.Key findings reveal that:(1)Temperature increases accelerate phenological stages but differentially impact polyphenols,suppressing anthocyanins and flavonols while promoting tannins;(2)Precipitation variability induces water stress that can enhance anthocyanin content under moderate drought but reduce quality during extreme events;(3)Declining sunshine duration may limit polyphenol production,though certain cultivars(e.g.,Beibinghong)exhibit adaptability to low light conditions.The region’s unique climatic trends-stronger winter warming and reduced summer precipitation-paradoxically offer potential benefits by extending the growing season while minimizing heat stress during critical ripening periods.It is highlighted how V.amurensis,with its cold hardiness and naturally high polyphenol content(notably anthocyanins and resveratrol),could become increasingly valuable under climate change.However,strategic adaptation through cultivar selection,vineyard management,and stress-responsive breeding will be critical to maintain wine quality.This synthesis provides a framework for understanding climate-polyphenol dynamics in cool-climate viticulture and outlines research priorities to safeguard the future of Northeast China’s distinctive wine industry.展开更多
In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SL...In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SLE and RTE)were obtained through ethanol reflux extraction and macroporous resin purification,and then formulated with maltodextrin and erythritol to prepare a composite solid beverage.This beverage exhibited excellent antioxidant capabilities.At a concentration of 1 mg/mL,the scavenging rates of DPPH,ABTS,and hydroxyl radicals reached 82.4%,94.6%,and 49.2%,respectively.Network pharmacology indicated that quercetin andβ-sitosterol could modulate lipid metabolism pathways.Moreover,the beverage showed potential for lipid-lowering.Its cholate adsorption capacity was 589.4±2.9 mg/g at pH 7.0,and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL.However,a 60-day storage stability test revealed that the moisture content approached 5%,likely due to polyphenol-flavonoid reactivity,extending dissolution time to 30.88 s.These changes were attributed to polyphenols(with flavonoids as the core active subclass,and non-flavonoids such as phenolic acids as auxiliary),resulting in color alterations and reduced solubility.This study confirmed the dual functions of the SL-RT beverage in antioxidant and lipid-lowering aspects.Nevertheless,it also pointed out the need to optimize the formula and process to enhance stability,providing an important basis for the development of stable functional beverages.展开更多
Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols...Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols.In this study,in vitro simulated gastrointestinal digestion and colonic fermentation models were used to study the release profile and metabolism of BPs of oat bran.Significantly higher level of BPs was released during in vitro colon fermentation(3.05 mg GAE/g)than in gastrointestinal digestion(0.54 mg GAE/g).Five polyphenols were detected via LC-MS and their possible conversion pathways were speculated.Released BPs exhibited chemical antioxidant capacity.16S rRNA sequencing further revealed that Clostridium butyricum,Enterococcus faecalis,Bacteroides acidifaciens were the key bacteria involved in the release of BPs,and this was verified by whole-cell transformation.Our results helped to explain the possible mechanism of the health benefits of BPs in whole grains.展开更多
Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity.In practical application,the research of food combinat...Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity.In practical application,the research of food combinations is more significant than a specific food component.This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley extract.We also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and scoparone.In vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m L.The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 cells.The results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,respectively.ALT and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4.展开更多
Akkermansia muciniphila play an important in ameliorating obesity but is not allowed for direct consumption in most countries.To date,microbiota-directed foods selectively promote the targeted human gut microbes,provi...Akkermansia muciniphila play an important in ameliorating obesity but is not allowed for direct consumption in most countries.To date,microbiota-directed foods selectively promote the targeted human gut microbes,providing a strategy for A.muciniphila enhancement.Multiple studies have indicated the potential regulation of the polyphenol on A.muciniphila.Therefore,a polyphenol screening based on A.muciniphila upregulation was performed in mice.Chlorogenic acid(CGA)exhibited a greater response to A.muciniphila upregulation.Furthermore,we found that CGA did not directly promote A.muciniphila growth or mucin secretion.Microbiome and metabolomics revealed that the increased abundance of A.muciniphila resulted from the inhibition of CGA on Desulfovibrio and Alistipes and the influence of docosahexaenoic acid,β-hydroxybutyrate,and N-acetyl-lactosamine.Finally,to confirm the regulation of CGA on A.muciniphila under disease conditions,high-fat diet-fed mice were established.The results showed CGA promoted A.muciniphila growth,and we expectedly found that CGA suppressed the augment in body weight of mice,significantly attenuated adipose tissue abnormality,provided liver protection and improved gut barrier integrity.These results suggest that CGA inhibits the development of obesity.Overall,our results indicate that microbiota-directed food is a promising approach for the treatment of obesity.展开更多
Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity ag...Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157:H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.展开更多
基金financially supporting this work through the Large Research Group Project under Grant no.R.G.P.2/509/45
文摘Polyphenols,a diverse group of naturally occurring compounds found in plants,have garnered significant attention for their potential therapeutic properties in treating neurodegenerative diseases(NDs).The Wnt/β-catenin(WβC)signaling pathway,a crucial player in neurogenesis,neuronal survival,and synaptic plasticity,is involved in several cellular mechanisms related to NDs.Dysregulation of this pathway is a hallmark in the development of various NDs.This study explores multiple polyphenolic compounds,such as flavonoids,stilbenes,lignans,and phenolic acids,and their potential to protect the nervous system.It provides a comprehensive analysis of their effects on the WβC pathway,elucidating their modes of action.The study highlights the dual function of polyphenols in regulating and protecting the nervous system,providing reassurance about the research benefits.This review provides a comprehensive analysis of the results obtained from both in vitro studies and in vivo research,shedding light on how these substances influence the various components of the pathway.The focus is mainly on the molecular mechanisms that allow polyphenols to reduce oxidative stress,inflammation,and apoptotic processes,ultimately improving the function and survival of neurons.This study aims to offer a thorough understanding of the potential of polyphenols in targeting the WβC signaling pathway,which could lead to the development of innovative therapeutic options for NDs.
基金supported by the Fundamental Research Funds for the Central Universities(No.22120230104).
文摘High-entropy alloy(HEA)nanoparticles(NPs)have attracted great attention in electrocatalysis due to their tailorable complex compositions and unique properties.Herein,we introduce Fe,Co,Ni,Cr and Mn into the metal-polyphenol coordination system to prepare HEA NPs enclosed in N-doped carbon(FeCoNiCrMn)with great potential for catalyzing oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).The unique high-entropy structural characteristics in FeCoNiCrMn facilitate effective interplay between metal species,leading to improved ORR(E_(1/2)=0.89 V)and OER(η=330 mV,j=10 mA·cm^(−2))activity.Additionally,FeCoNiCrMn exhibits excellent open-circuit voltage(1.523 V),power density(110 mW·cm^(−2))and long-term durability,outperforming Pt/C+IrO_(2) electrodes as a cathode catalyst in Zn-air batteries(ZABs).Such polyphenol-assisted alloying method broadens and simplifies the development of HEA electrocatalysts for high-performance ZABs.
基金supported by the Shanghai Rising-Star Program(24QB2702300)Shanghai Municipal Commission of Science and Technology(22N51900100).
文摘Acetaminophen(APAP)used as an antipyretic and analgesic agent can cause acute liver injury(AILI)under overdose.Sanghuangporous vaninii is an edible fungus with abundant metabolites exhibits excellent hepatoprotective activities,but the effect for AILI is not yet fully understood.In this study,the polyphenol rich extract from S.vaninii(PSV)was prepared,with a total phenolic content of 75.72%and 34 compounds.The data of hepatoprotection indicated that PSV obviously alleviated the hepatocellular injury induced by APAP in vivo and in vitro.The protective mechanism of PSV against APAP-induced AILI might be attributed to the activating NRF2/GPX4 pathway.Based on network pharmacology analysis,the active components of PSV such as caffeic acid,osmundacetone and hispolone played a key role in hepatoprotection of PSV.Consequently,this study highlights the protection of PSV on AILI,which provides new insight into bioactivities of S.vaninii.
文摘Quinoa is pseudo cereal and considered as full nutritional food for its functional ingredients such as peptides,polysaccharides,saponins and polyphenols.Up to now,over 20 phenolic compounds have been reported in quinoa,and these polyphenols are related to anti-cancer,anti-inflammatory,anti-obesity,anti-diabetic and cardioprotective activities.Recently,more and more attentions are focused on quinoa in the food and pharmaceutical fields.Many new products such as bakery,beverage and meat product made from quinoa are popular in the market.This article presents a review of the literature on the function and application of polyphenols in quinoa.The review will benefit the researchers working with nutrition,functional diets of quinoa.
基金supported by the National Natural Science Foundation Committee of China(81971212,81601129)Shenyang Young and Middle-aged Technological Innovation Talent Support Program(RC210268)Liaoning Province Natural Science Foundation(2023-MS-153).
文摘Epilepsy is a devastating neurological condition that affects over 70 million people worldwide.Apart from idiopathic epilepsy,numerous diseases have been reported to cause secondary epilepsy.Polyphenols,ubiquitously existing in fruits and vegetables,has become a promising neuroprotective candidate against multiple neurological abnormalities due to its interplay with gut microbiome composed of the bacterial population in gastrointestinal system.This bioactive compound is also being tested for the management of epileptogenesis.However,there is still insufficient evidence from mechanistic studies to demonstrate the causal relationship between anti-epileptic effect of polyphenols and microbial alteration.In this review,we provide the brief overview of epilepsy,polyphenols and gut microbiota.We then analysis the association of polyphenols and gut microbiota particularly in the epilepsy and symptomatic seizures-induced by epilepsy related disorders.We also discuss the possibility for the combined application with probiotics to improve the bioavailability and bioaccessibility of polyphenols for expanding its anti-epileptic function.We finally summarize the therapeutic potential of anti-seizure polyphenols through affecting gut microbiome,and propose that the anti-epileptic effect of polyphenols may be tightly relevant to intestinal bacterial flora for overcoming epilepsy and its comorbidities simultaneously.Therefore,the dietary polyphenols targeting specific bacteria may achieve the outcome of‘one stone,two birds’.
基金Supported by the Ministry of Science,Technological Development and Innovation of the Republic of Serbia,No.451-03-137/2025-03/200111 and No.451-03-137/2025-03/200146.
文摘Depression is a common psychiatric condition with a considerable influence on global health.Although current pharmacotherapeutic choices are of indisputable relevance,there is sustained interest in natural medicines for treating depressive symptoms.Polyphenols,a class of plant-derived natural compounds,have received attention due to their possible neuroprotective and anti-depressive properties.In this review,we aimed to present the intricate mechanisms through which polyphenols modulate inflammation,neurogenesis,oxidative stress,and the gut microbiota.Consumption of polyphenol-rich diets is emerging as a non-invasive,natural,and cost-effective approach to supporting brain health,with potential influence on depressive symptoms.While there have been promising results,more research is needed to draw solid conclusions regarding the medicinal potential of polyphenols.
基金supported by 2024 Liaoning Province Graduate Education Teaching Reform Research Project(LNYJG2024251).
文摘The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking.The TCMSP pharmacology database was used to screen the polyphenols present in sea buckthorn,and then the SwissTargetPrediction and Uniprot databases were used to obtain the potential targets of sea buckthorn polyphenols,which were supplemented by the literature.In total,7 polyphenols and 154 potential targets were obtained.Through GeneCards,OMIM database,1358 hyperlipidemia-related targets were collected.We found that there were 101 targets at the intersection of components and diseases.Through GO and KEGG enrichment analysis,27 core targets were obtained,which were AKT1,TNF,TP53,IL-6,etc.in order of degree value.174 pathways were obtained from KEGG enrichment analysis,including AGE-RAGE signaling pathway in diabetic complications,fl uid shear stress and atherosclerosis,lipid and atherosclerosis,etc.The molecular docking of the main components to the targets was performed using OpenBabelGUI,AutoDockTools-1.5.6 software.Finally,the results were visualized using Cytoscape 3.9.1 software.The molecular docking results showed that sea buckthorn polyphenols have good binding ability with the key targets.Among them,such as quercetin and kaempferol,have good binding ability with TNF,TP53 and IL-6.For example,TNF binds to quercetin with a binding energy of-5.34 kcal/mol and to kaempferol with a binding energy of-6.22 kcal/mol;TP53 binds to kaempferol with a binding energy of-5.32 kcal/mol;IL-6 binds to quercetin with a binding energy of-5.62 kcal/mol,etc.Therefore,the network pharmacology study showed that the treatment of hyperlipidemia by sea buckthorn polyphenols can be realized by multi-component-multi-target-multi-pathway together,which provides some reference for the later study of sea buckthorn polyphenols in the treatment of hyperlipidemia.
基金the National Natural Science Foundation of China(No.5220031085)the Postdoctoral Research Project in Henan Province(No.HN2022054)+2 种基金the Key Scientific Research Projects of Colleges and Universities in Henan Province(No.23A430009)the State Key Laboratory of Polymer Materials Engineering(No.sklpme2022-4-03)the Key Technologies R&D Program of Henan Province(No.242102230131)。
文摘The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint materials.However,the pursuit of sustained antibacterial efficacy remains a formidable challenge.In this context,we proposed a novel strategy that leverages swelling and erosion mechanisms to facilitate drug release of drug-loaded ultrahigh molecular weight polyethylene(UHMWPE),thereby ensuring its long-lasting antibacterial performance.Polyethylene oxide(PEO),a hydrophilic polymer with fast hydrating ability and high swelling capacity,was incorporated in UHMWPE alongside the antibacterial tea polyphenol(epigallocatechin gallate,EGCG as representative).The swelling of PEO enhanced water infiltration into the matrix,while the erosion of PEO balanced the release of the encapsulated EGCG,resulting in a steady release.The behavior was supported by the EGCG release profiles and the corresponding fitted release kinetic models.As demonstrated by segmented antibacterial assessments,the antibacterial efficiency was enhanced 2to 3 times in the PEO/EGCG/UHMWPE composite compared to that of EGCG/UHMWPE.Additionally,the PEO/EGCG/UHMWPE composite exhibited favorable biocompatibility and mechanical performance,making it a potential candidate for the development of drug-releasing joint implants to combat prosthetic bacterial infections.
基金supported by the National Natural Science Foundation of China(U22A20515&32102578)the National Key R&D Program of China(2023YFD1302300)+1 种基金the Science and Technology Research Project of Education Department of Jiangxi Province(GJJ191140)the Doctoral Foundation of Nanchang Normal University(NSBSJJ2019002)。
文摘The absence of trace amounts of natural bioactive compounds with important biological activities in traditional dietary models for global farm animals,coupled with an incomplete theoretical system for animal nutrition,has led to unbalanced and inadequate animal nutrition.This deficiency has adversely impacted animal health and the ecological environment,presenting formidable challenges to the advancement of the swine breeding industry in various countries around the world toward high-quality development.Recently,due to the ban of antibiotics for growth promotion in swine diets,botanical active compounds have been extensively investigated as feed additives.Polyphenols represent a broad group of plant secondary metabolites.They are natural,non-toxic,pollution-free,and highly reproducible compounds that have a wide range of physiological functions,such as antioxidant,anti-inflammatory,immunomodulatory,antiviral,antibacterial,and metabolic activities.Accordingly,polyphenols have been widely studied and used as feed additives in swine production.This review summarizes the structural characteristics,classification,current application situation,general properties of polyphenols,and the latest research advances on their use in swine production.Additionally,the research and application bottlenecks and future development of plant polyphenols in the animal feed industry are reviewed and prospected.This review aims to stimulate the in-depth study of natural plant polyphenols and the research and development of related products in order to promote the green,healthy,and high-quality development of swine production,while also providing ideas for the innovation and development in the theoretical system of animal nutrition.
文摘Apple polyphenols are widely present in apple pulp,peel,kernel,and leaves.They are natural bioactive substances with a variety of health functions.This review describes the antioxidant functions of anthocyanins,quercetin,phlorizin,chlorogenic acid,and epicatechin in apple polyphenols through the regulation of signal pathways and transcription factors,and the inhibition or induction of enzymes.Massive food applications of apple polyphenols are summarized,including excellent color protection of fruits and vegetables,meat preservation,preparation of composite packaging fi lms as active ingredients,enhancement of nutritional value as functional ingredients,and improvement of food taste and physical stability.This review would provide a reference for the exploration of apple polyphenol resources and its development in the food field.
文摘Emerging evidence highlights the potential of bioactive compounds,particularly polyphenols,as adjunctive therapeutic agents in the treatment of pancreatic cancer(PC),one of the most aggressive malignancies.This review focuses on epigallocatechin gallate(EGCG)and resveratrol due to their extensively documented anticancer activity,favorable safety profiles,and their unique ability to modulate multiple signaling pathways relevant to pan-creatic tumorigenesis.Among polyphenols,these two have shown superior anti-cancer activity,epigenetic regulatory effects,and synergy with standard chemotherapies in preclinical pancreatic cancer models.Resveratrol exhibits anti-proliferative effects by modulating key signaling pathways,including phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt),nuclear factor kappa-B(NF-κB),and tumor protein 53(p53).EGCG exerts anti-cancer activity by targeting multiple cellular processes,such as oxidative stress reduction,and suppression of inflammatory mediators like Interleukin-6(IL-6)and Tumor Necrosis Factor-α(TNF-α).Both EGCG and resveratrol exert anti-pancreatic cancer effects partly through direct interactions with cell surface receptors and modulation of intracellular cascades.EGCG targets the 67 kDa laminin receptor(67LR),which is overexpressed in pancreatic cancer cells,triggering apoptosis,cyclic guanosine monophosphate(cGMP)production and activation of the PKCδ/acid sphingomyelinase(ASM)cascade.Resveratrol inhibits insulin-like growth factor-1 receptor(IGF-1R)activation of the PI3K/Akt and Wnt signaling pathways,while concurrently activating tumor suppressor p53.These interactions suppress proliferation,promote apoptosis,and reduce epithelial-mesenchymal transition(EMT),thereby limiting tumor progression.Both polyphenols enhance chemosensitivity and reduce resistance to conventional therapies,including gemcitabine,by modulating drug transporters and apoptotic pathways.Furthermore,their epigenetic influence,particularly via DNA methylation and histone modification,suggests a broader role in pancreatic cancer prevention.Understanding the roles and mechanisms of resveratrol and EGCG in pancreatic cancer provides valuable insights into novel treatment strategies.The integration of polyphenols into conventional therapeutic approaches may offer new hope for improving patient outcomes.
基金supported by the National Natural Science Foundation of China(32001679 and 31871801)the Science and Technology Research of Shaanxi Province(2021QFY07-03)+1 种基金supported by the Fundamental Research Funds for the Central Universities(GK202103098)the Scientific and Technological Achievements Commercialization Program of Shaanxi(2023-YDCGZH-13)。
文摘Gut microbiota regulate the activation of adipose browning,which promote energy dissipation and combat diet-induced obesity.Pomegranate peel polyphenols(PPPs)have been shown to reduce obesity,regulate lipid metabolism in adipose tissue,and modulate the composition of gut microbiota in animal fed high-fat diet(HFD).However,the role of gut microbiota in the improvement of obesity by PPPs has not been elucidated.In current study,we applied antibiotics to inhibit gut microbiota in mice fed HFD and treated with PPPs.The results showed that the inhibition of gut microbiota impair the effect of PPPs on reducing obesity and promoting adipose browning,and change the fecal metabolomic profiles in respond to PPPs.Moreover,the inhibition of gut microbiota supressed the promotive effects of PPPs on the levels of Akkermansia and microbiota-related metabolites,such as urolithin A,short-chain fatty acids(SCFAs),and bile acids(BAs),which were associated with activating adipose browning.Therefore,our results suggested that the presence of gut microbiota is essential for PPPs to ameliorate HFD-induced obesity.The related bacteria or metabolites generated by the interaction between PPPs and microbiota promote adipose browning and facilitate the beneficial effects of PPPs.
基金supported by the Liaoning Provincial Department of Education(No.LJ212510163023)Liaoning Provincial Natural Science Foundation(No.2024-MSLH-443).
文摘This study employs combined network pharmacology and molecular docking approaches to investigate the potential mechanisms by which Erigeron breviscapus polyphenols inhibit liver fibrosis.Active compounds were identified through literature mining,with targets predicted using TCMSP,PubChem,SwissTarget,and SwissADME databases.Liver fibrosis-related targets were retrieved from GeneCards,OMIM,and TTD.Following rigorous screening,12 bioactive polyphenolic compounds and 117 corresponding targets were identified,intersecting with 8,375 liver fibrosis targets to yield 67 common targets.Protein-protein interaction analysis revealed 80 key targets(e.g.,EGFR,ESR1,PTGS2).GO and KEGG analyses indicated enrichment in 352 biological terms and 50 pathways,including chemical carcinogenesis receptor activation and steroid hormone biosynthesis.Molecular docking confirmed effective binding affinity between the top four compounds(by degree value)and their respective targets.In summary,the results of this study indicate that Erigeron breviscapus can inhibit the development of liver fibrosis and related diseases through multiple components,targets,and pathways.This study provides a solid theoretical basis for the research of Erigeron breviscapus in the field of anti liver fibrosis.
基金supported by the Key Program of Heilongjiang Provincial Natural Science Foundation of China(ZD2020C008)the National Natural Science Foundation of China(32071519)+2 种基金the Heilongjiang University Graduate Innovation Research Project(YJSCX2022-031HLJU)the Qiqihar Science and Technology Plan Joint Guidance Project(LSFGG-2022037)Scientific Research Project of Ecological Environment Protection of Heilongjiang Provincial Department of Ecological Environment(HST2022TR004)。
文摘During obesity treatment,clinicians have been burdened by the ever-increasing number of patients and the side effects of drug treatment.Finding new plant-derived active ingredients as an intervention for obesity has become a research focus.In this study,Plackett-Burman and Central Composite Design experiments,plant-wide target metabolomics technology,simulated human colon fermentation test,and cell experiment in vitro were successively used to investigate the effects of fermented and unfermented polyphenols(FP and UP,respectively)of blue honeysuckle on obesity.After the optimization of the response surface method,polyphenol content((1878.08±14.53)mg/100 g)increased by 2.63 times after Lactobacillus rhamnosus 6224 and Saccharomyces cerevisiae W5 mixed fermentation.There were 23 significantly different metabolites were observed in FP and UP.Of them,7 different metabolites were significantly and positively correlated with short-chain fatty acids(SCFAs),whereas negatively correlated with lipopolysaccharides(LPS).The contents of these 7 metabolites were significantly increased in FP.Furthermore,the effects of FP and UP on the SCFA biosynthesis pathway were different.Cell experiments revealed that polyphenols can significantly promote the secretion of gastrointestinal endogenous hormones and insulin and inhibit interleukin-6 levels.Linear regression analysis revealed SCFAs had a positive regulatory effect on glycolipid metabolism,whereas LPS had a negative effect.Overall,these crucial findings provide an economical and simple method for obtaining blue honeysuckle polyphenols and laying a theoretical foundation for its application in the therapeutic intervention against obesity.
文摘Climate change is significantly altering viticultural practices worldwide,with profound implications for the accumulation of polyphenolic compounds that determine wine’s sensory and health properties.This review summarizes the effects of climate change,particularly rising temperatures,shifting precipitation patterns,and altered light conditions-on polyphenol synthesis in Vitis amurensis(V.amurensis)grapes from Northeast China,the country’s highest-latitude wine region.Key findings reveal that:(1)Temperature increases accelerate phenological stages but differentially impact polyphenols,suppressing anthocyanins and flavonols while promoting tannins;(2)Precipitation variability induces water stress that can enhance anthocyanin content under moderate drought but reduce quality during extreme events;(3)Declining sunshine duration may limit polyphenol production,though certain cultivars(e.g.,Beibinghong)exhibit adaptability to low light conditions.The region’s unique climatic trends-stronger winter warming and reduced summer precipitation-paradoxically offer potential benefits by extending the growing season while minimizing heat stress during critical ripening periods.It is highlighted how V.amurensis,with its cold hardiness and naturally high polyphenol content(notably anthocyanins and resveratrol),could become increasingly valuable under climate change.However,strategic adaptation through cultivar selection,vineyard management,and stress-responsive breeding will be critical to maintain wine quality.This synthesis provides a framework for understanding climate-polyphenol dynamics in cool-climate viticulture and outlines research priorities to safeguard the future of Northeast China’s distinctive wine industry.
文摘In the context of a surging demand for functional foods,this study utilized Sophora japonica L.(SL)and Rosa rugosa Thunb.(RT),which are rich in polyphenols(with flavonoids as the core subclass).High-purity extracts(SLE and RTE)were obtained through ethanol reflux extraction and macroporous resin purification,and then formulated with maltodextrin and erythritol to prepare a composite solid beverage.This beverage exhibited excellent antioxidant capabilities.At a concentration of 1 mg/mL,the scavenging rates of DPPH,ABTS,and hydroxyl radicals reached 82.4%,94.6%,and 49.2%,respectively.Network pharmacology indicated that quercetin andβ-sitosterol could modulate lipid metabolism pathways.Moreover,the beverage showed potential for lipid-lowering.Its cholate adsorption capacity was 589.4±2.9 mg/g at pH 7.0,and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL.However,a 60-day storage stability test revealed that the moisture content approached 5%,likely due to polyphenol-flavonoid reactivity,extending dissolution time to 30.88 s.These changes were attributed to polyphenols(with flavonoids as the core active subclass,and non-flavonoids such as phenolic acids as auxiliary),resulting in color alterations and reduced solubility.This study confirmed the dual functions of the SL-RT beverage in antioxidant and lipid-lowering aspects.Nevertheless,it also pointed out the need to optimize the formula and process to enhance stability,providing an important basis for the development of stable functional beverages.
基金the support from the research grants by National Natural Science Foundation of China(32202051)National Key R&D Program of China(2022YFF1100104)the Major Project of Inner Mongolia Science and Technology Department,China(2021ZD0002)。
文摘Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols.In this study,in vitro simulated gastrointestinal digestion and colonic fermentation models were used to study the release profile and metabolism of BPs of oat bran.Significantly higher level of BPs was released during in vitro colon fermentation(3.05 mg GAE/g)than in gastrointestinal digestion(0.54 mg GAE/g).Five polyphenols were detected via LC-MS and their possible conversion pathways were speculated.Released BPs exhibited chemical antioxidant capacity.16S rRNA sequencing further revealed that Clostridium butyricum,Enterococcus faecalis,Bacteroides acidifaciens were the key bacteria involved in the release of BPs,and this was verified by whole-cell transformation.Our results helped to explain the possible mechanism of the health benefits of BPs in whole grains.
基金financially supported by the National Key Research and Development Program of China(2021YFD2100904)the National Natural Science Foundation of China(31871729,32172147)+2 种基金the Modern Agriculture key Project of Jiangsu Province of China(BE2022317)the Modern Agricultural Industrial Technology System Construction Project of Jiangsu Province of China(JATS[2021]522)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity.In practical application,the research of food combinations is more significant than a specific food component.This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley extract.We also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and scoparone.In vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m L.The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 cells.The results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,respectively.ALT and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4.
基金supported by the Natural Science Foundation of Jiangsu Province[BK20200084]the National Natural Science Foundation of China[No.32122067 and 32021005]the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Akkermansia muciniphila play an important in ameliorating obesity but is not allowed for direct consumption in most countries.To date,microbiota-directed foods selectively promote the targeted human gut microbes,providing a strategy for A.muciniphila enhancement.Multiple studies have indicated the potential regulation of the polyphenol on A.muciniphila.Therefore,a polyphenol screening based on A.muciniphila upregulation was performed in mice.Chlorogenic acid(CGA)exhibited a greater response to A.muciniphila upregulation.Furthermore,we found that CGA did not directly promote A.muciniphila growth or mucin secretion.Microbiome and metabolomics revealed that the increased abundance of A.muciniphila resulted from the inhibition of CGA on Desulfovibrio and Alistipes and the influence of docosahexaenoic acid,β-hydroxybutyrate,and N-acetyl-lactosamine.Finally,to confirm the regulation of CGA on A.muciniphila under disease conditions,high-fat diet-fed mice were established.The results showed CGA promoted A.muciniphila growth,and we expectedly found that CGA suppressed the augment in body weight of mice,significantly attenuated adipose tissue abnormality,provided liver protection and improved gut barrier integrity.These results suggest that CGA inhibits the development of obesity.Overall,our results indicate that microbiota-directed food is a promising approach for the treatment of obesity.
基金supported by National Natural Science Foundation of China(31972021)R&D Projects in Key Areas of Guangdong Province(2019B020212003)+4 种基金the Science and Technology Program of Guangzhou,China(202206010177)Guangdong key research and development program(2021B0202060001)Foshan and agricultural academy cooperation projectGuangdong Modern Agriculture project(2022KJ117)Aquatic Products Center Project of GAAS。
文摘Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157:H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.
