Vitis vinifera var.L.País leaf extract,previously characterized,was microencapsulated by emulsion-solvent evaporation method using two different procedures:the conventional dropwise and micromixer device.The poly...Vitis vinifera var.L.País leaf extract,previously characterized,was microencapsulated by emulsion-solvent evaporation method using two different procedures:the conventional dropwise and micromixer device.The poly-3-hydroxybutyrate-co-3-hydroxyvalerate(PHBV)was used as encapsulating polymer.In addition,the effect of extraction time and ethanol:water ratio on the yield and the content of bioactive compounds of grape leaf extract obtained by Soxhlet method was determined using the face-centered central composite design.The highest bioactive compound were obtained using a 70:30 ethanol:water solvent ratio during 4 cycles.This extract is mainly composed of cinnamic acid,quercetin-3-glucoside and quercetin-3-glucorinide,containing 3.56,2.08 and 1.19 mg g^(-1)extract,respectively.The total polyphenol content determined by Folin Ciocalteu method was 164±14 mg GAE g^(-1)extract.The microparticles(2-3μm)had spherical shape with rough and porous surface.The encapsulation efficiency was 15.1%and 60.6%for the PHBV microparticles prepared using conventional dropwise and micromixer device,respectively.The antioxidant activity present in the original extract was preserved around 50%-80%and 20%-70%in the loaded microparticles.The best results were achieved when grape leaf extract was encapsulated using micromixer device.The cell viability assay in 3T3-L1 fibroblasts,showed that loaded microparticles are not cytotoxic.In conclusion micromixer device showed to be effective for the microencapsulation of grape leaf extract using PHBV as encapsuling polymer.展开更多
基金financed by CIPA,ANID Regional[Grant Number R23F0005].GORE˜NUBLE[Grant Number FIC 40044432-0].
文摘Vitis vinifera var.L.País leaf extract,previously characterized,was microencapsulated by emulsion-solvent evaporation method using two different procedures:the conventional dropwise and micromixer device.The poly-3-hydroxybutyrate-co-3-hydroxyvalerate(PHBV)was used as encapsulating polymer.In addition,the effect of extraction time and ethanol:water ratio on the yield and the content of bioactive compounds of grape leaf extract obtained by Soxhlet method was determined using the face-centered central composite design.The highest bioactive compound were obtained using a 70:30 ethanol:water solvent ratio during 4 cycles.This extract is mainly composed of cinnamic acid,quercetin-3-glucoside and quercetin-3-glucorinide,containing 3.56,2.08 and 1.19 mg g^(-1)extract,respectively.The total polyphenol content determined by Folin Ciocalteu method was 164±14 mg GAE g^(-1)extract.The microparticles(2-3μm)had spherical shape with rough and porous surface.The encapsulation efficiency was 15.1%and 60.6%for the PHBV microparticles prepared using conventional dropwise and micromixer device,respectively.The antioxidant activity present in the original extract was preserved around 50%-80%and 20%-70%in the loaded microparticles.The best results were achieved when grape leaf extract was encapsulated using micromixer device.The cell viability assay in 3T3-L1 fibroblasts,showed that loaded microparticles are not cytotoxic.In conclusion micromixer device showed to be effective for the microencapsulation of grape leaf extract using PHBV as encapsuling polymer.
