Objective:To isolate the phenolic compounds obtained from the dried roots ofPolygonum multiflorum and investigate their pharmacological activities.Methods:The chemical constituents were isolated and purified by combin...Objective:To isolate the phenolic compounds obtained from the dried roots ofPolygonum multiflorum and investigate their pharmacological activities.Methods:The chemical constituents were isolated and purified by combining them with a macroporous resin(DM-8),MCI gel,and Sephadex LH-20 and by performing ODS column chromatography.Their structures were elucidated by 1D and 2D NMR analyses,as well as mass spectrometry.The isolated compounds were evaluated to determine their hepatoprotective andα-glucosidase inhibitory activities in vitro.Results:Two phenolic compounds,namely,polygonimitin E(1)and polygonimitin F(2),were isolated from the dried roots of P.multiflorum.Compound 2(10μmol/L)only showed moderate hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced HepG2 cell damage.Unfortunately,these two compounds exhibited noα-glucosidase inhibitory activity.Conclusion:Compounds 1 and 2 were new compounds.Compound 2 could be one of the potential hepatoprotective constituents of P.multiflorum.展开更多
文摘目的研究何首乌Polygonum multiflorum的化学成分。方法采用大孔吸附树脂(DM-8型)、Sephadex LH-20、反相硅胶柱色谱和液相制备等多种色谱技术进行分离纯化,采用核磁共振等波谱技术进行结构鉴定。结果从何首乌提取物55%乙醇洗脱部位中分离得到10个化合物,分别鉴定为thunberginol C 6-O-β-D-glucopyranoside(1)、对羟基苯甲醛(2)、反式-N-咖啡酰酪胺(3)、(Z)-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(4)、(E)-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(5)、白藜芦醇-3-O-β-D-(2″-O-没食子酰)-葡萄糖苷(6)、(E)-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-(3″-没食子酰)-葡萄糖苷(7)、苜蓿素-7-O-β-D-葡萄糖苷(8)、torachrysone-8-O-β-D-glucoside(9)、大黄素-8-O-β-D-葡萄糖苷(10)。结论化合物1为新化合物,命名为何首乌丁素。化合物3和8为首次从何首乌中分离得到。
基金The authors are grateful to the members of the analytical group of the State Key Laboratory of Bioactive Substances and Functions of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences(CAMS)and Peking Union Medical College,Beijing,China for the spectroscopic data measurements and bioactivity tests.This project was financially supported by the National Natural Science Foundation of China(Nos.81703665,81773874,81973476 and 81903807)the National Major Scientific and Technological Special Project for"Significant New Drugs Development"(2018ZX09735006).
文摘Objective:To isolate the phenolic compounds obtained from the dried roots ofPolygonum multiflorum and investigate their pharmacological activities.Methods:The chemical constituents were isolated and purified by combining them with a macroporous resin(DM-8),MCI gel,and Sephadex LH-20 and by performing ODS column chromatography.Their structures were elucidated by 1D and 2D NMR analyses,as well as mass spectrometry.The isolated compounds were evaluated to determine their hepatoprotective andα-glucosidase inhibitory activities in vitro.Results:Two phenolic compounds,namely,polygonimitin E(1)and polygonimitin F(2),were isolated from the dried roots of P.multiflorum.Compound 2(10μmol/L)only showed moderate hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced HepG2 cell damage.Unfortunately,these two compounds exhibited noα-glucosidase inhibitory activity.Conclusion:Compounds 1 and 2 were new compounds.Compound 2 could be one of the potential hepatoprotective constituents of P.multiflorum.
