Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mec...Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.展开更多
Idesia polycarpa is a kind of woody oil plant with high quality and yield. It is suitable to be planted in mountainous area. In this study,polyethylene glycol（ PEG） stress environment was used to evaluate the growth...Idesia polycarpa is a kind of woody oil plant with high quality and yield. It is suitable to be planted in mountainous area. In this study,polyethylene glycol（ PEG） stress environment was used to evaluate the growth response of I. polycarpa under drought conditions. The results showed that the seed germination parameters were inhibited to different degrees,and the growth and respiration rate of the seedlings increased slightly under the low concentration of PEG,and obviously decreased when the concentration of PEG was over 15%.展开更多
Idesia polycarpa Maxim.var.vestita Diels is an important oil plant with high economic value.In order to improve seed germination and to cultivate high quality seedlings,we set out to identify an optimal medium for its...Idesia polycarpa Maxim.var.vestita Diels is an important oil plant with high economic value.In order to improve seed germination and to cultivate high quality seedlings,we set out to identify an optimal medium for its seedling growth in a plastic cave tray(plug seedling).In this study,four different media including M_(1)(soil),M_(2)(peat∶perlite=2∶1),M_3(peat),and M_4(peat∶perlite∶vermiculite=2∶1∶1)were tested in industrialized plug seedlings.The seedling growth of Idesia polycarpa var.vestita including plant height,crown diameter,leaf number,ground diameter,the relative chlorophyll contents of leaves,etc.was measured.The results indicated that the medium M_(2) was the best for increasing seedling height,crown diameter,leaf counts,ground diameter,and the relative chlorophyll contents of leaves.Therefore,medium M_(2) is recommended in the industrialized nursery process for the seedling growth of Idesia polycarpa var.vestita.展开更多
Defatted seed,pulp and whole fruit of Idesia Polycarpa Maxin.(I.Polycarpa)are the by-products of the oil extraction process,but have attracted little attention as natural sources of bioactive phenolics.This study aime...Defatted seed,pulp and whole fruit of Idesia Polycarpa Maxin.(I.Polycarpa)are the by-products of the oil extraction process,but have attracted little attention as natural sources of bioactive phenolics.This study aimed to clarify the profiles of phenolic compounds in the extracts from these by-products and their contribution to the anti-inflammatory activity.Twenty-one phenolic compounds were identified,belonging mainly to salicinoid analogues and p-coumaroyl derivatives.Idescarpin was the predominant phenolics in all extracts,especially in seed extract.Nearly half the p-coumaroyl derivatives presented relatively high content in the extracts of pulp and whole fruit.All extracts inhibited the production of nitric oxide(NO)and the mRNA expressions of inducible nitric oxide synthase(iNOS),tumor necrosis factor(TNF-α),and interleukin(IL)-6;and suppressed the accumulation of reactive oxygen species(ROS)along with the restoration of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities.Seed extract exerted better potential than the pulp and whole fruit extracts.Furthermore,correlation analysis demonstrated that idescarpin and idesin were the crucial phenolics against inflammation,whereas some p-coumaroyl derivatives contributed to regulating oxidative stress.These findings suggested that phenolic extracts from defatted I.Polycarpa fruit and its parts,especially seed,could be utilized as inflammation inhibitors in the functional food and cosmetics industries.展开更多
This study presents a comprehensive bibliometric analysis of Idesia polycarpa research,mapping the global scientific landscape from 1994 to 2024 using the WoSCC and CNKI databases.Analysis of 496 publications reveals ...This study presents a comprehensive bibliometric analysis of Idesia polycarpa research,mapping the global scientific landscape from 1994 to 2024 using the WoSCC and CNKI databases.Analysis of 496 publications reveals a field dominated by China,which contributes 90.2%of the international(WoSCC)literature,with Henan Agricultural University being the most productive institution.Co-citation analysis identifies"seed"biology and lipid metabolism as the intellectual foundation,whereas keyword evolution highlights"fruit"and associated gene regulation as the current research frontier.While fatty acids remain a central theme,a distinct divergence is observed:international research delves into molecular mechanisms,while domestic studies in China National Knowledge Infrastructure(CNKI)are strongly application-driven,focusing on oil content and germination rate.This study synthesizes these trends to provide a strategic framework for prioritizing future research and optimizing resource allocation,thereby supporting the sustainable development of I.polycarpa as a multifunctional species.展开更多
Idesia polycarpa(I.polycarpa)is a newcomer of edible oil crops in China,with rich oil in ripe fruits.Phenolic compounds in six I.polycarpa oil(whole fruit oil from Leibo,LWFO;pulp oil from Leibo,LPO;seed oil from Leib...Idesia polycarpa(I.polycarpa)is a newcomer of edible oil crops in China,with rich oil in ripe fruits.Phenolic compounds in six I.polycarpa oil(whole fruit oil from Leibo,LWFO;pulp oil from Leibo,LPO;seed oil from Leibo,LSO;whole fruit oil from Puan,PWFO;pulp oil from Puan,PPO;and seed oil from Puan,PSO)were analyzed using untargeted metabolomics,and their roles in antioxidant activity were also investigated.Thirty-one phenolic compounds were tentatively identified in I.polycarpa oils,primarily belonging to salicinoid analogues and p-coumaroyl derivatives;and their contents varied greatly from trace to 85.84 mg/kg oil(idescarpin in LSO).Multivariate analyses clearly differentiated the oils of I.polycarpa fruit in according to fruit parts and geographical origins,with twelve phenolics as the discrimative markers.Furthermore,phenolic extracts from the oils,especially LSO and PPO,exerted strong scavenging activity toward DPPH and ABTS radicals.Eight phenolics such as dimethyl coniferin,2-hydroxyphenol-1-O-β-D-glucopyranosyl-(6->1)-α-L-rhamnopyranoside,1-O-(2-hydroxyethyl)-4-O-p-coumaroyl-glucopyranose,tanegoside A,1-O-(2-hydroxyphenyl)-4-O-p-coumaroyl-β-D-glucopyranose,mono-O-acetylated p-coumaroyl idesin,and p-coumaroyl salicortin were the crucial compounds that played important roles in antioxidant potential.Overall,the findings suggested these phenolics as chemical markers for the differentiation and functional evaluation of I.polycarpa oils.展开更多
文摘Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.
基金Supported by Scientific Research Project of State Ethnic Affair Commission(14HBZ007)
文摘Idesia polycarpa is a kind of woody oil plant with high quality and yield. It is suitable to be planted in mountainous area. In this study,polyethylene glycol（ PEG） stress environment was used to evaluate the growth response of I. polycarpa under drought conditions. The results showed that the seed germination parameters were inhibited to different degrees,and the growth and respiration rate of the seedlings increased slightly under the low concentration of PEG,and obviously decreased when the concentration of PEG was over 15%.
基金Supported by the grant from forestry science and technology innovation research project of Anhui province,China(AHLYCX-2021-07)。
文摘Idesia polycarpa Maxim.var.vestita Diels is an important oil plant with high economic value.In order to improve seed germination and to cultivate high quality seedlings,we set out to identify an optimal medium for its seedling growth in a plastic cave tray(plug seedling).In this study,four different media including M_(1)(soil),M_(2)(peat∶perlite=2∶1),M_3(peat),and M_4(peat∶perlite∶vermiculite=2∶1∶1)were tested in industrialized plug seedlings.The seedling growth of Idesia polycarpa var.vestita including plant height,crown diameter,leaf number,ground diameter,the relative chlorophyll contents of leaves,etc.was measured.The results indicated that the medium M_(2) was the best for increasing seedling height,crown diameter,leaf counts,ground diameter,and the relative chlorophyll contents of leaves.Therefore,medium M_(2) is recommended in the industrialized nursery process for the seedling growth of Idesia polycarpa var.vestita.
基金sincerely appreciate the support provided by the Na-tional Natural Science Foundation of China(32272242)Zhejiang Province Key Research and Development Projects(2024C04014,2025C04041)Zhejiang Provincial Natural Science Foundation of China(LTGN24C200011).
文摘Defatted seed,pulp and whole fruit of Idesia Polycarpa Maxin.(I.Polycarpa)are the by-products of the oil extraction process,but have attracted little attention as natural sources of bioactive phenolics.This study aimed to clarify the profiles of phenolic compounds in the extracts from these by-products and their contribution to the anti-inflammatory activity.Twenty-one phenolic compounds were identified,belonging mainly to salicinoid analogues and p-coumaroyl derivatives.Idescarpin was the predominant phenolics in all extracts,especially in seed extract.Nearly half the p-coumaroyl derivatives presented relatively high content in the extracts of pulp and whole fruit.All extracts inhibited the production of nitric oxide(NO)and the mRNA expressions of inducible nitric oxide synthase(iNOS),tumor necrosis factor(TNF-α),and interleukin(IL)-6;and suppressed the accumulation of reactive oxygen species(ROS)along with the restoration of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities.Seed extract exerted better potential than the pulp and whole fruit extracts.Furthermore,correlation analysis demonstrated that idescarpin and idesin were the crucial phenolics against inflammation,whereas some p-coumaroyl derivatives contributed to regulating oxidative stress.These findings suggested that phenolic extracts from defatted I.Polycarpa fruit and its parts,especially seed,could be utilized as inflammation inhibitors in the functional food and cosmetics industries.
基金Supported by the Guizhou Provincial Foundation for Excellent Scholars Program(QKHPTRC-GCC[2023]067)the Guizhou Provincial Major Scientific and Technological Program(QKZDZXZ[2024]023)the Projects of Guizhou Provincial Department of Education(QJJ[2024]324).
文摘This study presents a comprehensive bibliometric analysis of Idesia polycarpa research,mapping the global scientific landscape from 1994 to 2024 using the WoSCC and CNKI databases.Analysis of 496 publications reveals a field dominated by China,which contributes 90.2%of the international(WoSCC)literature,with Henan Agricultural University being the most productive institution.Co-citation analysis identifies"seed"biology and lipid metabolism as the intellectual foundation,whereas keyword evolution highlights"fruit"and associated gene regulation as the current research frontier.While fatty acids remain a central theme,a distinct divergence is observed:international research delves into molecular mechanisms,while domestic studies in China National Knowledge Infrastructure(CNKI)are strongly application-driven,focusing on oil content and germination rate.This study synthesizes these trends to provide a strategic framework for prioritizing future research and optimizing resource allocation,thereby supporting the sustainable development of I.polycarpa as a multifunctional species.
基金support provided by the National Natural Science Foundation of China(32272242)Zhejiang Province Key Research and Development Projects(2024C04014,2025C04041)Zhejiang Provincial Natural Science Foundation of China(LTGN24C200011).
文摘Idesia polycarpa(I.polycarpa)is a newcomer of edible oil crops in China,with rich oil in ripe fruits.Phenolic compounds in six I.polycarpa oil(whole fruit oil from Leibo,LWFO;pulp oil from Leibo,LPO;seed oil from Leibo,LSO;whole fruit oil from Puan,PWFO;pulp oil from Puan,PPO;and seed oil from Puan,PSO)were analyzed using untargeted metabolomics,and their roles in antioxidant activity were also investigated.Thirty-one phenolic compounds were tentatively identified in I.polycarpa oils,primarily belonging to salicinoid analogues and p-coumaroyl derivatives;and their contents varied greatly from trace to 85.84 mg/kg oil(idescarpin in LSO).Multivariate analyses clearly differentiated the oils of I.polycarpa fruit in according to fruit parts and geographical origins,with twelve phenolics as the discrimative markers.Furthermore,phenolic extracts from the oils,especially LSO and PPO,exerted strong scavenging activity toward DPPH and ABTS radicals.Eight phenolics such as dimethyl coniferin,2-hydroxyphenol-1-O-β-D-glucopyranosyl-(6->1)-α-L-rhamnopyranoside,1-O-(2-hydroxyethyl)-4-O-p-coumaroyl-glucopyranose,tanegoside A,1-O-(2-hydroxyphenyl)-4-O-p-coumaroyl-β-D-glucopyranose,mono-O-acetylated p-coumaroyl idesin,and p-coumaroyl salicortin were the crucial compounds that played important roles in antioxidant potential.Overall,the findings suggested these phenolics as chemical markers for the differentiation and functional evaluation of I.polycarpa oils.
