AIM:To identify key genes and inflammatory signaling pathways involved in the anti-inflammatory effects of Hedysarum polybotrys polysaccharide(HPS)in a rat model of endotoxin-induced uveitis(EIU).METHODS:EIU was induc...AIM:To identify key genes and inflammatory signaling pathways involved in the anti-inflammatory effects of Hedysarum polybotrys polysaccharide(HPS)in a rat model of endotoxin-induced uveitis(EIU).METHODS:EIU was induced in Wistar rats through subcutaneous injection of lipopolysaccharide(LPS,200μg)and the rats were then randomly assigned to EIU group(n=5)and the HPS intervention group(n=5).HPS(400 mg/kg,intraperitoneally)or its carrier was administered 24h and 1h prior to EIU induction.Eyes were examined and enucleated 24h post-induction,and total RNA was extracted from the iris-ciliary body.Gene expression microarrays were used to identify differentially expressed genes(DEGs),followed by bioinformatics analyses,including gene ontology(GO)and pathway analysis.Key findings were not experimentally validated at the mRNA or protein level.RESULTS:A total of 322 DEGs were identified,comprising 254 mRNA and 68 lncRNA genes.GO analysis revealed significant functional categories,including response to LPS.Pathway analysis identified key signaling pathways involved in uveitis,such as cytokine-cytokine receptor interactions.Notably,16 mRNA and 7 lncRNA DEGs emerged as central nodes in the gene correlation network.CONCLUSION:HPS exerts its anti-inflammatory effects through coordinated signaling pathways,offering insights into potential therapeutic targets for managing uveitis.展开更多
Objective： To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen （PCNA） gene in erythroleukemia cell...Objective： To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen （PCNA） gene in erythroleukemia cell line K562. Methods： The effect of total flavonoids of Hedysarum po/ybotry on K562 cell line survival was determined by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium （MTT） reduction assay. The time- and dose- dependent manner was also observed. The cell cycle and apoptosis were analyzed with flow cytometry （FCM）. The immunocytochemistry method was applied to quantitatively analyze the effects of flavonoids of Hedysarum polybotry on changes p21Ras and PCNA gene expressions. Results： Flavonoids of Hedysarum polybotry （20-100 g/mL） significantly inhibited the proliferation of K562 cells in a time- and dose-dependent manner. After K562 cells were cultured for 48 h, total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition （P〈0.01）, indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at Go/G1 and G2/M phases. Compared with the control group, p21Ras and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry （40 and 80 μg/mL, respectively） for 48 h. Conclusion： The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry, which might be related to cells arresting.展开更多
基金Supported by the National Natural Science Fundation of China(No.82101107No.81471575).
文摘AIM:To identify key genes and inflammatory signaling pathways involved in the anti-inflammatory effects of Hedysarum polybotrys polysaccharide(HPS)in a rat model of endotoxin-induced uveitis(EIU).METHODS:EIU was induced in Wistar rats through subcutaneous injection of lipopolysaccharide(LPS,200μg)and the rats were then randomly assigned to EIU group(n=5)and the HPS intervention group(n=5).HPS(400 mg/kg,intraperitoneally)or its carrier was administered 24h and 1h prior to EIU induction.Eyes were examined and enucleated 24h post-induction,and total RNA was extracted from the iris-ciliary body.Gene expression microarrays were used to identify differentially expressed genes(DEGs),followed by bioinformatics analyses,including gene ontology(GO)and pathway analysis.Key findings were not experimentally validated at the mRNA or protein level.RESULTS:A total of 322 DEGs were identified,comprising 254 mRNA and 68 lncRNA genes.GO analysis revealed significant functional categories,including response to LPS.Pathway analysis identified key signaling pathways involved in uveitis,such as cytokine-cytokine receptor interactions.Notably,16 mRNA and 7 lncRNA DEGs emerged as central nodes in the gene correlation network.CONCLUSION:HPS exerts its anti-inflammatory effects through coordinated signaling pathways,offering insights into potential therapeutic targets for managing uveitis.
基金Supported by the Natural Science Foundation for Middle-AgedYoung Scientist of Gansu Province(No.YS031-A21-015)
文摘Objective： To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen （PCNA） gene in erythroleukemia cell line K562. Methods： The effect of total flavonoids of Hedysarum po/ybotry on K562 cell line survival was determined by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium （MTT） reduction assay. The time- and dose- dependent manner was also observed. The cell cycle and apoptosis were analyzed with flow cytometry （FCM）. The immunocytochemistry method was applied to quantitatively analyze the effects of flavonoids of Hedysarum polybotry on changes p21Ras and PCNA gene expressions. Results： Flavonoids of Hedysarum polybotry （20-100 g/mL） significantly inhibited the proliferation of K562 cells in a time- and dose-dependent manner. After K562 cells were cultured for 48 h, total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition （P〈0.01）, indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at Go/G1 and G2/M phases. Compared with the control group, p21Ras and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry （40 and 80 μg/mL, respectively） for 48 h. Conclusion： The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry, which might be related to cells arresting.
