Background: Wrinkles are characterized by changes in the organization and structure of the dermis. Human wrinkle fibroblasts (WF) have a different functional behaviour in comparison with normal-aged fibroblasts (NF). ...Background: Wrinkles are characterized by changes in the organization and structure of the dermis. Human wrinkle fibroblasts (WF) have a different functional behaviour in comparison with normal-aged fibroblasts (NF). Decreases in migration capacities and collagen I synthesis are observed. Mitochondrial function is impaired with an increase in lactate production during aging. Sculptra? (poly-L-lactic acid: PLLA), a biodegradable synthetic polymer, is used for subcutaneous volume restoration. Thus we decided to investigate different fibroblast functions when placed in contact with PLLA. Objectives: The potential of PLLA to compensate for the reduction of metabolic activity, to restore the migration capacity of WF and to inhibit the lactate production, was investigated and compared to NF. Methods: Two different skin samples were used from each of the three women’s facelift (one inside a face wrinkle and one from normal aged skin). Collagen I, lactate productions and proliferation capacities were investigated on monolayer cultures. Migration properties were evaluated using three-dimensional collagen lattices. Results: PLLA increased collagen I synthesis, restored migration capacities and tended to decrease lactate production in WF, whereas PPLA stimulated proliferation in NF and tended to improve the migration of NF. Conclusion: These results suggested that PLLA from Sculptra? acted as a stimulus for collagen production in WF and that it is suitable for correcting skin depressions, such as wrinkles.展开更多
Poly-L-lactic acid(PLLA)has become a popular treatment for body skin laxity,especially in female patients;however,research on its use in male patients remains limited,particularly in addressing changes in the gluteal ...Poly-L-lactic acid(PLLA)has become a popular treatment for body skin laxity,especially in female patients;however,research on its use in male patients remains limited,particularly in addressing changes in the gluteal region due to aging.This case report investigates the efficacy of PLLA in treating skin laxity in the gluteal area of three male patients aged 36,41,and 50 years who were treated at the Antony Barbosa Institute between May 2023 and June 2024.The Male Gluteal Contour Method^(TM):C-Tech Collagen Boost was employed using a C-shaped injection technique with an 18G×70 mm cannula.Patients underwent four sessions of PLLA injections,reconstituted with bacteriostatic sterile water and lidocaine,at 4–6-week intervals.Improvements in the gluteal contour and skin laxity were noted in all patients,with increased collagen production contributing to enhanced muscle definition and firmness.Minimal side effects such as mild bruising,swelling,and tenderness were reported,and no nodules were observed.This study highlights the importance of tailored treatment plans that cater to the specific needs of middle-age and older men.The C-shaped injection technique allowed for an even distribution of the biostimulator while respecting male anatomy.PLLA is a promising nonsurgical solution for improving male gluteal contour and skin quality,although further research with larger groups is warranted to establish more definitive guidelines for its use in male gluteal augmentation.展开更多
A high Mw of PLLA was synthesized, it intended to be used in the manufacture ofbioabsorbable screw applied in bone fracture internal fixation. The optical reactionconditions have been discussed.
Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydro...Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material.展开更多
文摘Background: Wrinkles are characterized by changes in the organization and structure of the dermis. Human wrinkle fibroblasts (WF) have a different functional behaviour in comparison with normal-aged fibroblasts (NF). Decreases in migration capacities and collagen I synthesis are observed. Mitochondrial function is impaired with an increase in lactate production during aging. Sculptra? (poly-L-lactic acid: PLLA), a biodegradable synthetic polymer, is used for subcutaneous volume restoration. Thus we decided to investigate different fibroblast functions when placed in contact with PLLA. Objectives: The potential of PLLA to compensate for the reduction of metabolic activity, to restore the migration capacity of WF and to inhibit the lactate production, was investigated and compared to NF. Methods: Two different skin samples were used from each of the three women’s facelift (one inside a face wrinkle and one from normal aged skin). Collagen I, lactate productions and proliferation capacities were investigated on monolayer cultures. Migration properties were evaluated using three-dimensional collagen lattices. Results: PLLA increased collagen I synthesis, restored migration capacities and tended to decrease lactate production in WF, whereas PPLA stimulated proliferation in NF and tended to improve the migration of NF. Conclusion: These results suggested that PLLA from Sculptra? acted as a stimulus for collagen production in WF and that it is suitable for correcting skin depressions, such as wrinkles.
文摘Poly-L-lactic acid(PLLA)has become a popular treatment for body skin laxity,especially in female patients;however,research on its use in male patients remains limited,particularly in addressing changes in the gluteal region due to aging.This case report investigates the efficacy of PLLA in treating skin laxity in the gluteal area of three male patients aged 36,41,and 50 years who were treated at the Antony Barbosa Institute between May 2023 and June 2024.The Male Gluteal Contour Method^(TM):C-Tech Collagen Boost was employed using a C-shaped injection technique with an 18G×70 mm cannula.Patients underwent four sessions of PLLA injections,reconstituted with bacteriostatic sterile water and lidocaine,at 4–6-week intervals.Improvements in the gluteal contour and skin laxity were noted in all patients,with increased collagen production contributing to enhanced muscle definition and firmness.Minimal side effects such as mild bruising,swelling,and tenderness were reported,and no nodules were observed.This study highlights the importance of tailored treatment plans that cater to the specific needs of middle-age and older men.The C-shaped injection technique allowed for an even distribution of the biostimulator while respecting male anatomy.PLLA is a promising nonsurgical solution for improving male gluteal contour and skin quality,although further research with larger groups is warranted to establish more definitive guidelines for its use in male gluteal augmentation.
文摘A high Mw of PLLA was synthesized, it intended to be used in the manufacture ofbioabsorbable screw applied in bone fracture internal fixation. The optical reactionconditions have been discussed.
基金supported by the National Natural Science Foundation of China,No.31071222Jilin Province Science and Technology Development Project in China,No.20080738the Frontier Interdiscipline Program of Norman Bethune Health Science Center of Jilin University in China,No.2013106023
文摘Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material.
