This study explores the combination of ultrasound technology with a detection algorithm to categorize flow regimes in bubble columns used for aeration in aquaculture.An ultrasonic velocity profiler is used to obtain t...This study explores the combination of ultrasound technology with a detection algorithm to categorize flow regimes in bubble columns used for aeration in aquaculture.An ultrasonic velocity profiler is used to obtain the standard deviation of the bubble velocity distributed throughout the column.The bubble velocity data for three known flow regimes were used to develop a probability density function(PDF)classification model.The experimental apparatus consisted of a circular tank equipped with a bubble generator and gas hold-up monitoring systems.The flow regimes of the experimental fluid were determined,and the classification was conducted via the PDF method.The results demonstrate that the classification accuracy is not lower than that of traditional machine learning methods.展开更多
The membrane aeration biofilm reactor(MABR)represents an innovative approach to wastewater treatment,integrating gas separation membranes with biofilm process and demonstrating effectiveness in treating wastewater ric...The membrane aeration biofilm reactor(MABR)represents an innovative approach to wastewater treatment,integrating gas separation membranes with biofilm process and demonstrating effectiveness in treating wastewater rich in ammonia nitrogen.In this system,hollow fiber membranes are essential,serving as a substrate for biofilm attachment while facilitating oxygen transfer to microorganisms through aeration,hydrophobic microporous membranes are utilized in MABR applications.This study focuses on the use of poly-4-methyl-1-pentene(PMP)hollow fiber membranes,which exhibit superior oxygen permeation capabilities compared to traditional hydrophobic microporous membranes.To overcome the challenges posed by the hydrophobic nature and low bubble point of PMP microporous membranes,a hydrophilic modification was conducted using dopamine/poly(ethyleneimine)(DOPA/PEI)co-deposition to enhance microbial adhesion on the membrane surface.The composite membrane modified with DOPA/PEI exhibited an approximately 20%higher NH_(4)^(+)-N removal efficiency than the unmodified membrane.These findings suggest that the incorporation of DOPA/PEI significantly improves MABR performance,underscoring its potential for further research and development in membrane technology for MABR.展开更多
Microbial activity may influence phosphorus (P) deposit and release at the water sediment interface. The properties of DO (dissolved oxygen), pH, P fractions (TE Ca-E Fe-R OE IP), and APA (alkaline phospfiatase...Microbial activity may influence phosphorus (P) deposit and release at the water sediment interface. The properties of DO (dissolved oxygen), pH, P fractions (TE Ca-E Fe-R OE IP), and APA (alkaline phospfiatase activity) at the water sediment interface were measured to investigate microbial activity variations in surface sediment under conditions of two-month intermittent aeration in overlying water. Results showed that DO and TP of overlying water increased rapidly in the first week and then decreased gradually after 15 day of intermittent aeration. Microorganism metabolism in surface sediment increased pH and decreased DO and TP in the overlying water. After two-month intermittent aeration, APA and OP from surface sediment (0-2 cm) were both significantly higher than those from bottom sediment (6-8 cm) (p 〈 0.05), and surface sediment Fe-P was transferred to OP during the course of microorganism reproduction on the surface sediment. These results suggest that microbial activity and microorganism biomass from the surface sediment were higher than those from bottom sediment after two-month intermittent aeration in the overlying water.展开更多
Three lab-scale vertical-flow constructed wetlands (VFCWs), including the non-aerated (NA), intermittently aerated (IA) and continuously aerated (CA) ones, were operated at different hydraulic loading rates (...Three lab-scale vertical-flow constructed wetlands (VFCWs), including the non-aerated (NA), intermittently aerated (IA) and continuously aerated (CA) ones, were operated at different hydraulic loading rates (HLRs) to evaluate the effect of artificial aeration on the treatment efficiency of heavily polluted river water. Results indicated that artificial aeration increased the dissolved oxygen (DO) concentrations in IA and CA, which significantly favored the removal of organic matter and NH4+-N. The DO grads caused by intermittent aeration formed aerobic and anoxic regions in IA and thus promoted the removal of total nitrogen (TN). Although the removal efficiencies of CODEr, NH4+-N and TN in the three VFCWs all decreased with an increase in HLR, artificial aeration enhanced the reactor resistance to the fluctuation of pollutant loadings. The maximal removal efficiencies of CODEr, NH4+-N and total phosphorus (TP) (i.e., 81%, 87% and 37%, respectively) were observed in CA at 19 cm/day HLR, while the maximal TN removal (i.e., 57%) was achieved in IA. Although the improvement of artificial aeration on TP removal was limited, this study has demonstrated the feasibility of applying artificial aeration to VFCWs treating polluted river water, particularly at a high HLR.展开更多
In order to clarify the effects of aeration on root nitrogen metabolism in rice seedlings,rice cultivars Guodao 6 (indica) and Xiushui 09 (japonica) were investigated for root growth,the activities of glutamine sy...In order to clarify the effects of aeration on root nitrogen metabolism in rice seedlings,rice cultivars Guodao 6 (indica) and Xiushui 09 (japonica) were investigated for root growth,the activities of glutamine synthetase (GS),glutamic acid-pyruvic acid transaminase (GPT) and glutamic acid oxaloacetate transaminase (GOT),the nitrate (NO 3-N) concertration,the contents of free amino acids and soluble sugar in root under hydroponics with continuous aeration treatment.The results showed that rice seedlings grown in oxygenation solutions had higher root dry matter,longer root length,stronger root activity and larger root absorption area compared with the control.In addition,the contents of soluble sugar,root vigor and the activities of GS,GOT and GPT in the aeration solutions were higher than those in the control.The results also indicated that the activities of enzymes involved in root nitrogen metabolism of Xiushui 09 were enhanced by aeration,however,there was no significant influence on root nitrogen metabolism of Guodao 6,which suggested that effect of oxygenation on rice root nitrogen metabolism might be genotype-specific.展开更多
The aim of this study was to uncover ways to mitigate greenhouse gas(GHG) emissions and reduce energy consumption during the composting process. We assessed the effects of different aeration rates(0, 0.18, 0.36, an...The aim of this study was to uncover ways to mitigate greenhouse gas(GHG) emissions and reduce energy consumption during the composting process. We assessed the effects of different aeration rates(0, 0.18, 0.36, and 0.54 L/(kg dry matter(dm)·min)) and methods(continuous and intermittent) on GHG emissions. Pig feces and corn stalks were mixed at a ratio of 7:1. The composting process lasted for 10 weeks, and the compost was turned approximately every 2 weeks. Results showed that both aeration rate and method significantly affected GHG emissions. Higher aeration rates increased NH3 and N2O losses,but reduced CH4 emissions. The exception is that the CH4 emission of the passive aeration treatment was lower than that of the low aeration rate treatment. Without forced aeration,the CH4 diffusion rates in the center of the piles were very low and part of the CH4 was oxidized in the surface layer. Intermittent aeration reduced NH3 and CH4 losses, but significantly increased N2 O production during the maturing periods. Intermittent aeration increased the nitrification/denitrification alternation and thus enhanced the N2 O production. Forced aeration treatments had higher GHG emission rates than the passive aeration treatment. Forced aeration accelerated the maturing process, but could not improve the quality of the end product. Compared with continuous aeration, intermittent aeration could increase the O2 supply efficiency and reduced the total GHG emission by 17.8%, and this reduction increased to 47.4% when composting was ended after 36 days.展开更多
Adding organic amendments to stimulate the biodegradation of pesticides is a subject of ongoing interest. The effect of sewage sludge on the bioremediation of dichlorodiphenyltrichloroethane(DDT) and hexachlorocyclo...Adding organic amendments to stimulate the biodegradation of pesticides is a subject of ongoing interest. The effect of sewage sludge on the bioremediation of dichlorodiphenyltrichloroethane(DDT) and hexachlorocyclohexane(HCH) contaminated soil was investigated in bench scale experiments,and intermittent aeration strategy was also used in this study to form an anaerobic–aerobic cycle. Bioremediation of DDT and HCH was enhanced with the addition of sewage sludge and the intermittent aeration. The removal rates of HCH and DDT were raised by 16.8%–80.8% in 10 days. Sewage sludge increased the organic carbon content from 6.2 to218 g/kg,and it could also introduce efficient degradation microbes to soil,including Pseudomonas sp.,Bacillus sp. and Sphingomonas sp. The unaerated phase enhanced the anaerobic dechlorination of DDT and HCH,and anaerobic removal rates of β-HCH,o,p′-DDT and p,p′-DDT accounted for more than 50% of the total removal rates,but the content of α-HCH declined more in the aerobic phase.展开更多
When municipal solid waste(MSW) with high content of food waste is landfilled, the rapid hydrolysis of food waste results in the imbalance of anaerobic metabolism in the landfill layer, indicated by accumulation of vo...When municipal solid waste(MSW) with high content of food waste is landfilled, the rapid hydrolysis of food waste results in the imbalance of anaerobic metabolism in the landfill layer, indicated by accumulation of volatile fatty acids(VFA) and decrease of pH value. This occurrence could lead to long lag time before the initiation of methanogenesis and to the production of strong leachate. Simulated landfill columns with forced aeration, with natural ventilation, and with no aeration, were monitored regarding their organics degradation rate with leachate recirculation. Hydrolysis reactions produced strong leachate in the column with no aeration. With forced aeration, the produced VFA could be effectively degraded, leading to the reduction in COD of the leachate effluent since the week 3. The CH_4 in the landfill gas from the column with aeration rate of 0.39 m3/(m3·d) and frequency of twice/d, leachate recirculation rate of 12.2 mm/d and frequency of twice/d, could amount to 40%(v/v) after only 20 weeks. This amount had increased up to 50% afterward even with no aeration. Most of COD in the recirculated leachate was removed. Using natural ventilation, CH_4 could also be produced and the COD of the leachate effluent be reduced after 10 weeks of operation. However, the persistent existence of oxygen in the landfill layer yielded instability in methanogenesis process.展开更多
Despite lots of techniques in improving the heap leaching performance,many constraints on the industrial applications remain.We proposed a correspondingly effective and new idea of introducing forced aeration to impro...Despite lots of techniques in improving the heap leaching performance,many constraints on the industrial applications remain.We proposed a correspondingly effective and new idea of introducing forced aeration to improve the bad permeability and leaching effect of Yangla Copper Mine(YCM)during heap leaching.The dual-media theory was employed to study the impact mechanism of forced aeration on the variations of porous and fractured media during the column leaching experiments.An X-Ray Computed Tomography(CT)set was utilized to perform the pore imaging of the specimens and the fracture morphology of the particles within the columns was analyzed by Scanning Electron Microscope(SEM)as aeration rate(AR)changed.The results show that there exists copious fine particles within the heap of YCM,the particle size distribution of which is not reasonable.The forced aeration can not only promote the development of the porous and fractured structures but effectively break the blocked seepage paths.Then the leaching degree is improved and the seepage performance of the solute within the solution is enhanced.Therefore,the forced aeration is probable of making the leaching performance greatly improved.展开更多
Due to large-scale dredging operations, a large amount of sludge is inevitably produced. Large areas of land are occupied when the dredged sludge is discarded in the disposal site as waste material. The sludge dewater...Due to large-scale dredging operations, a large amount of sludge is inevitably produced. Large areas of land are occupied when the dredged sludge is discarded in the disposal site as waste material. The sludge dewatering with aeration-vacuum (SDAV) method is suit for treating the sludge with high water content and high clay content in the disposal site. The water in the sludge can be discharged out. The volume of the sludge can be reduced quickly, and the recycling of the land can be accelerated by this method. Most importantly, this technique is an efficient way to deal with clogging problems when pumping water from high water content, high clay content dredged sludge. Vacuum degree range tests, the aeration rate range tests, and the influencing factors of sludge dewatering behavior tests were conducted with a self-developed SDAV model test device. Sludge samples were taken from the South-to-North Water Diversion East Line Project in Huai’an White-Horse Lake disposal site, Jiangsu Province, China. The optimal range of vacuum degree and aeration rate were obtained through the test results, and the mechanisms for how the two factors work and how they affect the sludge dewatering behavior were analyzed. The suitable vacuum degree range in SDAV is below 50 kPa, and the suitable aeration rate is about 1.0 m3/h. The low-vacuum degree contributes to reduce the ad-sorption effect of micro-channels on soil particles in filter material and to maintain the arch structures. Aeration has the effects of expansion, disturbance, changing Reynolds number, and dynamic sieve separating. The pump quantity of water per meter of filter tube (m) has different change rules as the vacuum degree changes under different aeration rates. The reason is that the formed arch structures’ conformation and permeability differ greatly under different combined-conditions of vacuum degree and aeration rate. The optimal combined-condition for dewatering the sludge is 35 kPa with 1.0 m3/h.展开更多
Pilot scale experiments with forced aeration were conducted to estimate effects of aeration rates on the performance of composting penicillin mycelial dreg using sewage sludge as inoculation. Three aeration rates of 0...Pilot scale experiments with forced aeration were conducted to estimate effects of aeration rates on the performance of composting penicillin mycelial dreg using sewage sludge as inoculation. Three aeration rates of 0.15, 0.50 and 0.90 L/(min·kg) organic matter(OM) were examined. The principal physicochemical parameters were monitored during the 32 day composting period. Results showed that the higher aeration rate of 0.90 L/(min·kg) did not corresponded to a longer thermophilic duration and higher rates of OM degradation;but the lower aeration rate of 0.15 L/(min·kg) did induce an accumulation of NH+4-N contents due to the inhibition of nitrification. On the other hand, aeration rate has little effect on degradation of penicillin. The results show that the longest phase of thermophilic temperatures ≥ 55°C, the maximum NO-3-N content and seed germination, and the minimum C/N ratio were obtained with 0.50 L/(min·kg) OM. Therefore, aeration rates of0.50 L/(min·kg) OM can be recommended for composting penicillin mycelial dreg.展开更多
To improve the efficiency of ethanol production in a batch fermentation from sweet sorghum juice under a very high gravity(VHG)condition(~290 g/L of total sugar)by Saccharomyces cerevisiae NP01,repeatedbatch fermentat...To improve the efficiency of ethanol production in a batch fermentation from sweet sorghum juice under a very high gravity(VHG)condition(~290 g/L of total sugar)by Saccharomyces cerevisiae NP01,repeatedbatch fermentation under an aerated condition(2.5 vvm for the first 4 h during every cycle)was done in a5-L fermenter.The average ethanol concentration(P),productivity(Qp)and yield(Yp/s)for five successive cycles were 112.31 g/L,1.55 g/L·h^-1 and 0.44,respectively with 80.97%sugar consumption.To complete sugar consumption,the total sugar of the juice was reduced to a high gravity(HG)level(~240 g/L).The results showed that yeast extract was not necessary for ethanol production,and aeration during every other cycle i.e.,alternating cycles,was sufficient to promote both yeast growth and ethanol production.The average P,Qpand Yp/svalues for eight successive cycles with aeration during alternating cycles were97.58 g/L,1.98 g/Láh and 0.41,respectively with 91.21%sugar consumption.The total fatty acids in the yeast cells under the aerated condition were^50%higher than without aeration,irrespective the initial sugar concentration,whereas the ergosterol contents under aeration condition were^29%to 49%higher than those without aeration.展开更多
Instream aeration has been used as a supplement to secondary treatment or a substitute for tertiary treatment for meeting dissolved oxygen (DO) standards in rivers. Many studies have used water quality models to det...Instream aeration has been used as a supplement to secondary treatment or a substitute for tertiary treatment for meeting dissolved oxygen (DO) standards in rivers. Many studies have used water quality models to determine the number, location, and capacity of instream aeration stations (IASs) needed to meet DO standards in combination with other pollution control measures. DO concentrations have been improved in the North Shore Channel and North Branch Chicago River by the Devon Avenue IAS for more than 35 years. A study was initiated to determine whether it was better to rehabilitate or relocate this station and to determine appropriate operational guidance for the IAS at the selected location. A water quality model capable of simulating DO concentrations during unsteady flow was used to evaluate the proper location for an IAS and operational guidance for this IAS. Three test years, a dry year, a wet year, and an extreme year, were considered in the evaluation. The study found that the Devon Avenue IAS should be rehabilitated as this location performed as well as or better than any of 10 alternative locations. According to the new operational guidance for this IAS, the amount of time with blowers operating could be substantially reduced compared to traditional operations while at the same time the attainment of the DO standards could be increased. This study shows that a carefully designed modeling study is key to effective selection, location, and operation of IASs such that attainment of DO standards can be maximized while operation hours of blowers can be minimized.展开更多
Biological nutrient removal grows into complicated scenario due to the microbial consortium shift and kinetic competition between phosphorus(P)-accumulating and nitrogen(N)-removing microorganisms.In this study,three ...Biological nutrient removal grows into complicated scenario due to the microbial consortium shift and kinetic competition between phosphorus(P)-accumulating and nitrogen(N)-removing microorganisms.In this study,three sequential batch reactors with constant operational conditions except aeration patterns at 6 h cycle periods were tested.Intermittent aeration was applied to develop a robust nutrient removal system aimed to achieve high energy saving and removal efficiency.The results showed higher correspondence of Puptake,polymeric substance synthesis and glycogen degradation in intermittent-aeration with longer interval periods compared to continuous-aeration.Increasing the intermittentaeration duration from 25 to 50 min,resulted in higher process performance where the system exhibited approximately 30%higher nutrient removal.This study indicated that nutrient removal strongly depends on reaction phase configuration representing the importance of aeration pattern.The microbial community examined the variation in abundance of bacterial groups in suspended sludge,where the 50 min intermittent aeration,favored the growth of P-accumulating organisms and nitrogen removalmicrobial groups,indicating the complications related to nutrient removal systems.Successful intermittently aerated process with high capability of simple implementation to conventional systems by elemental retrofitting,is applicable for upgrading wastewater treatment plants.With aeration as a major operational cost,this process is a promising approach to potentially remove nutrients in high competence,in distinction to optimizing cost-efficacy of the system.展开更多
Aerated irrigation has been proven to increase crop production and quality, but studies on its environmental impacts are sparse. The effects of aeration and irrigation regimes on soil CO2 and N2O emissions in two cons...Aerated irrigation has been proven to increase crop production and quality, but studies on its environmental impacts are sparse. The effects of aeration and irrigation regimes on soil CO2 and N2O emissions in two consecutive greenhouse tomato rotation cycles in Northwest China were studied via the static closed chamber and gas chromatography technique. Four treatments, aerated deficit irrigation(AI1), non-aerated deficit irrigation(CK1), aerated full irrigation(AI2) and non-aerated full irrigation(CK2), were performed. The results showed that the tomato yield under aeration of each irrigation regime increased by 18.8% on average compared to non-aeration, and the difference was significant under full irrigation(P〈0.05). Full irrigation significantly increased the tomato yield by 23.9% on average in comparison to deficit irrigation. Moreover, aeration increased the cumulative CO2 emissions compared to non-aeration, and treatment effects were significant in the autumn-winter season(P〈0.05). A slight increase of CO2 emissions in the two seasons was observed under full irrigation(P〉0.05). There was no significant difference between aeration and non-aeration in soil N2O emissions in the spring-summer season, whereas aeration enhanced N2O emissions significantly in the autumn-winter season. Furthermore, full irrigation over the two seasons greatly increased soil N2O emissions compared to the deficit irrigation treatment(P〈0.05). Correlation analysis indicated that soil temperature was the primary factor influencing CO2 fluxes. Soil temperature, soil moisture and NO3^- were the primary factors influencing N2O fluxes. Irrigation coupled with particular soil aeration practices may allow for a balance between crop production yield and greenhouse gas mitigation in greenhouse vegetable fields.展开更多
In order to improve removal for nitrogen in a pilot-scale submerged membrane bioreactor(SMBR),intermittent aeration was conducted,and the effect on the treatment performance under four kinds of operation condition(...In order to improve removal for nitrogen in a pilot-scale submerged membrane bioreactor(SMBR),intermittent aeration was conducted,and the effect on the treatment performance under four kinds of operation condition(run 1,continuous aeration;run 2,60/60 min aeration on/off time;run 3,60/90 min aeration on/off time;run 4,60/75 min aeration on/off time) was evaluated.The results showed that depending on the specific on/off of the aeration time ratio,removal efficiency of nitrogen could be improved significantly,and the removal rates of total nitrogen(TN) under different operation conditions were 28.0%,59.5%,66.8% and 70.7%,respectively.There were no obvious differences for removal rates for CODCr and ammonia among different operation conditions.In general,intermittent aeration could be used as a feasible way to improve treatment performance for nitrogen in the SMBR.展开更多
Free surface flows aeration potential causing the in macro- and intermediate flow characteristics to vary roughness conditions have a high with slopes and discharges. The underlying mechanism of two-phase flow charact...Free surface flows aeration potential causing the in macro- and intermediate flow characteristics to vary roughness conditions have a high with slopes and discharges. The underlying mechanism of two-phase flow characteristics in macro- and intermediate roughness conditions were analyzed in an experimental setup assembled at the Laboratory of Hydraulic Protection of the Territory (PITLAB) of the University of Pisa, Italy. Crushed angular rocks and hemispherical boulders were used to intensify the roughness of the bed. Flow rates per unit width ranging between 0.03 m^2/s and 0.09 m^2/s and slopes between 0.26 and 0.46 were tested over different arrangements of a rough bed. Analyses were mainly carried out in the inner flow region, which consists of both bubbly and intermediate flow regions. The findings revealed that the two-phase flow properties over the rough bed were much affected by rough bed arrangements. Turbulence features of two-phase flows over the rough bed were compared with those of the stepped chute data under similar flow conditions. Overall, the results highlight the flow features in the inner layers of the two-phase flow, showing that the maximum turbulence intensity decreases with the relative submergence, while the bubble frequency distribution is affected by the rough bed elements.展开更多
Knowledge of the oxygen mass transfer of aerators under operational conditions in a full-scale wastewater treatment plant (WWTP) is meaningful for the optimization of WWTP, however, scarce to best of our knowledge. ...Knowledge of the oxygen mass transfer of aerators under operational conditions in a full-scale wastewater treatment plant (WWTP) is meaningful for the optimization of WWTP, however, scarce to best of our knowledge. Through analyzing a plug flow aeration tank in the Lucun WWTP, in Wuxi, China, the oxygenation capacity of fine-bubble aerators under process conditions have been measured in- situ using the off-gas method and the non-steady-state method. The off-gas method demonstrated that the aerators in different corridors in the aeration tank of WWTP had significantly different oxygen transfer performance; furthermore, the aerators in the same corridor shared almost equal oxygen transfer performance over the course of a day. Results measured by the two methods showed that the oxygen transfer performance of fine-bubble aerators in the aeration tank decreased dramatically compared with that in the clean water. The loss of oxygen transfer coefficient was over 50% under low-aeration conditions (aeration amount 〈 0.67 Nm 3 /hr). However, as the aeration amount reached 0.96 Nm 3 /hr, the discrepancy of oxygen transfer between the process condition and clean water was negligible. The analysis also indicated that the non-steady-state and off-gas methods resulted in comparable estimates of oxygen transfer parameters for the aerators under process conditions.展开更多
The potential for reducing greenhouse gas(GHG)emissions and energy consumption in wastewater treatment can be realized through intelligent control,with machine learning(ML)and multimodality emerging as a promising sol...The potential for reducing greenhouse gas(GHG)emissions and energy consumption in wastewater treatment can be realized through intelligent control,with machine learning(ML)and multimodality emerging as a promising solution.Here,we introduce an ML technique based on multimodal strategies,focusing specifically on intelligent aeration control in wastewater treatment plants(WWTPs).The generalization of the multimodal strategy is demonstrated on eight ML models.The results demonstrate that this multimodal strategy significantly enhances model indicators for ML in environmental science and the efficiency of aeration control,exhibiting exceptional performance and interpretability.Integrating random forest with visual models achieves the highest accuracy in forecasting aeration quantity in multimodal models,with a mean absolute percentage error of 4.4%and a coefficient of determination of 0.948.Practical testing in a full-scale plant reveals that the multimodal model can reduce operation costs by 19.8%compared to traditional fuzzy control methods.The potential application of these strategies in critical water science domains is discussed.To foster accessibility and promote widespread adoption,the multimodal ML models are freely available on GitHub,thereby eliminating technical barriers and encouraging the application of artificial intelligence in urban wastewater treatment.展开更多
Different cadmium(Cd)-accumulated rice genotypes(Erjiunan 1 and Fupin 36)were used to explore the effect of rice rhizosphere aeration on Cd uptake and accumulation.Aeration in the nutrient soluti on influe need the ag...Different cadmium(Cd)-accumulated rice genotypes(Erjiunan 1 and Fupin 36)were used to explore the effect of rice rhizosphere aeration on Cd uptake and accumulation.Aeration in the nutrient soluti on influe need the agronomic characteristics in duced by Cd-stress,such as the in creases of rice root length and root vigor,but the reductions of plant height and shoot dry weight.Aeration also alleviated the decreasing effects of Cd stress on antioxidant enzyme activities and soluble protein,malonaldehyde and nicotianamine contents in rice.Moreover,with aeration treatment,the accumulation and bioavailability of metal elements changed significantly,with a Cd increase and an Fe reduction in both rice genotypes.In addition,at the molecular level,aeration upregulated the expression of Fe-inducible genes(such as OsIRTI,OsNRAMPI,OsYSL15 and OsNAS3).Furthermore,as a Cd^(2+)/Fe^(2+) transporter,the high transcription level of OsNRAMPI can elevate the Cd uptake and translocation in rice due to the Fe reduction caused by aeration and Cd-exposure,which indicated that OsNRAMPI might play a crucial role in the effect of aeration on Cd uptake and accumulation.展开更多
基金supported by the Center of Excellence on Instru-mentation Technology and Automation(CEITA),Department of Instru-mentation and Electronics Engineering,Faculty of Engineering,King Mongkut’s University of Technology North Bangkok,Thailand。
文摘This study explores the combination of ultrasound technology with a detection algorithm to categorize flow regimes in bubble columns used for aeration in aquaculture.An ultrasonic velocity profiler is used to obtain the standard deviation of the bubble velocity distributed throughout the column.The bubble velocity data for three known flow regimes were used to develop a probability density function(PDF)classification model.The experimental apparatus consisted of a circular tank equipped with a bubble generator and gas hold-up monitoring systems.The flow regimes of the experimental fluid were determined,and the classification was conducted via the PDF method.The results demonstrate that the classification accuracy is not lower than that of traditional machine learning methods.
基金supported by the National Key Research and Development Program of China(2023YFB3810502)the National Natural Science Foundation of China(22078146)the Key Research and Development program of Anhui Province(2023h11020004).
文摘The membrane aeration biofilm reactor(MABR)represents an innovative approach to wastewater treatment,integrating gas separation membranes with biofilm process and demonstrating effectiveness in treating wastewater rich in ammonia nitrogen.In this system,hollow fiber membranes are essential,serving as a substrate for biofilm attachment while facilitating oxygen transfer to microorganisms through aeration,hydrophobic microporous membranes are utilized in MABR applications.This study focuses on the use of poly-4-methyl-1-pentene(PMP)hollow fiber membranes,which exhibit superior oxygen permeation capabilities compared to traditional hydrophobic microporous membranes.To overcome the challenges posed by the hydrophobic nature and low bubble point of PMP microporous membranes,a hydrophilic modification was conducted using dopamine/poly(ethyleneimine)(DOPA/PEI)co-deposition to enhance microbial adhesion on the membrane surface.The composite membrane modified with DOPA/PEI exhibited an approximately 20%higher NH_(4)^(+)-N removal efficiency than the unmodified membrane.These findings suggest that the incorporation of DOPA/PEI significantly improves MABR performance,underscoring its potential for further research and development in membrane technology for MABR.
基金supported by the National Major Science and Technology Project for Water Pollution Control and Management(No. 2008ZX07101-001,2009ZX07101-009)
文摘Microbial activity may influence phosphorus (P) deposit and release at the water sediment interface. The properties of DO (dissolved oxygen), pH, P fractions (TE Ca-E Fe-R OE IP), and APA (alkaline phospfiatase activity) at the water sediment interface were measured to investigate microbial activity variations in surface sediment under conditions of two-month intermittent aeration in overlying water. Results showed that DO and TP of overlying water increased rapidly in the first week and then decreased gradually after 15 day of intermittent aeration. Microorganism metabolism in surface sediment increased pH and decreased DO and TP in the overlying water. After two-month intermittent aeration, APA and OP from surface sediment (0-2 cm) were both significantly higher than those from bottom sediment (6-8 cm) (p 〈 0.05), and surface sediment Fe-P was transferred to OP during the course of microorganism reproduction on the surface sediment. These results suggest that microbial activity and microorganism biomass from the surface sediment were higher than those from bottom sediment after two-month intermittent aeration in the overlying water.
基金support from the Ministry of Environmental Protection (No.2008ZX07101-006-08)the Ministry of Science and Technology of China (No. 2009BAC57B02)
文摘Three lab-scale vertical-flow constructed wetlands (VFCWs), including the non-aerated (NA), intermittently aerated (IA) and continuously aerated (CA) ones, were operated at different hydraulic loading rates (HLRs) to evaluate the effect of artificial aeration on the treatment efficiency of heavily polluted river water. Results indicated that artificial aeration increased the dissolved oxygen (DO) concentrations in IA and CA, which significantly favored the removal of organic matter and NH4+-N. The DO grads caused by intermittent aeration formed aerobic and anoxic regions in IA and thus promoted the removal of total nitrogen (TN). Although the removal efficiencies of CODEr, NH4+-N and TN in the three VFCWs all decreased with an increase in HLR, artificial aeration enhanced the reactor resistance to the fluctuation of pollutant loadings. The maximal removal efficiencies of CODEr, NH4+-N and total phosphorus (TP) (i.e., 81%, 87% and 37%, respectively) were observed in CA at 19 cm/day HLR, while the maximal TN removal (i.e., 57%) was achieved in IA. Although the improvement of artificial aeration on TP removal was limited, this study has demonstrated the feasibility of applying artificial aeration to VFCWs treating polluted river water, particularly at a high HLR.
基金supported by the Provincial Natural Science Foundation of Zhejiang (Grant No. Y3100270)the Twelfth Five-Year Science and Technology Project (Grant No. 2011BAD16B14)
文摘In order to clarify the effects of aeration on root nitrogen metabolism in rice seedlings,rice cultivars Guodao 6 (indica) and Xiushui 09 (japonica) were investigated for root growth,the activities of glutamine synthetase (GS),glutamic acid-pyruvic acid transaminase (GPT) and glutamic acid oxaloacetate transaminase (GOT),the nitrate (NO 3-N) concertration,the contents of free amino acids and soluble sugar in root under hydroponics with continuous aeration treatment.The results showed that rice seedlings grown in oxygenation solutions had higher root dry matter,longer root length,stronger root activity and larger root absorption area compared with the control.In addition,the contents of soluble sugar,root vigor and the activities of GS,GOT and GPT in the aeration solutions were higher than those in the control.The results also indicated that the activities of enzymes involved in root nitrogen metabolism of Xiushui 09 were enhanced by aeration,however,there was no significant influence on root nitrogen metabolism of Guodao 6,which suggested that effect of oxygenation on rice root nitrogen metabolism might be genotype-specific.
基金supported by the National Natural Science Foundation of China (No. 41201282)part of the Chinese National Science and Technology Support Program (2012BAD14B01/06/18)Leshan Normal University Foundation Z1159
文摘The aim of this study was to uncover ways to mitigate greenhouse gas(GHG) emissions and reduce energy consumption during the composting process. We assessed the effects of different aeration rates(0, 0.18, 0.36, and 0.54 L/(kg dry matter(dm)·min)) and methods(continuous and intermittent) on GHG emissions. Pig feces and corn stalks were mixed at a ratio of 7:1. The composting process lasted for 10 weeks, and the compost was turned approximately every 2 weeks. Results showed that both aeration rate and method significantly affected GHG emissions. Higher aeration rates increased NH3 and N2O losses,but reduced CH4 emissions. The exception is that the CH4 emission of the passive aeration treatment was lower than that of the low aeration rate treatment. Without forced aeration,the CH4 diffusion rates in the center of the piles were very low and part of the CH4 was oxidized in the surface layer. Intermittent aeration reduced NH3 and CH4 losses, but significantly increased N2 O production during the maturing periods. Intermittent aeration increased the nitrification/denitrification alternation and thus enhanced the N2 O production. Forced aeration treatments had higher GHG emission rates than the passive aeration treatment. Forced aeration accelerated the maturing process, but could not improve the quality of the end product. Compared with continuous aeration, intermittent aeration could increase the O2 supply efficiency and reduced the total GHG emission by 17.8%, and this reduction increased to 47.4% when composting was ended after 36 days.
基金supported by the National Natural Science Foundation of China (No.41271478,41101463,and 41271479)the Special Fund for Scientific Research of China's Ministry of Land and Resources in the Public Interest (No.201111020)
文摘Adding organic amendments to stimulate the biodegradation of pesticides is a subject of ongoing interest. The effect of sewage sludge on the bioremediation of dichlorodiphenyltrichloroethane(DDT) and hexachlorocyclohexane(HCH) contaminated soil was investigated in bench scale experiments,and intermittent aeration strategy was also used in this study to form an anaerobic–aerobic cycle. Bioremediation of DDT and HCH was enhanced with the addition of sewage sludge and the intermittent aeration. The removal rates of HCH and DDT were raised by 16.8%–80.8% in 10 days. Sewage sludge increased the organic carbon content from 6.2 to218 g/kg,and it could also introduce efficient degradation microbes to soil,including Pseudomonas sp.,Bacillus sp. and Sphingomonas sp. The unaerated phase enhanced the anaerobic dechlorination of DDT and HCH,and anaerobic removal rates of β-HCH,o,p′-DDT and p,p′-DDT accounted for more than 50% of the total removal rates,but the content of α-HCH declined more in the aerobic phase.
基金The National Hi Tech Research and Development Program(863) of China(No. 2001AA644010 2003AA644020)
文摘When municipal solid waste(MSW) with high content of food waste is landfilled, the rapid hydrolysis of food waste results in the imbalance of anaerobic metabolism in the landfill layer, indicated by accumulation of volatile fatty acids(VFA) and decrease of pH value. This occurrence could lead to long lag time before the initiation of methanogenesis and to the production of strong leachate. Simulated landfill columns with forced aeration, with natural ventilation, and with no aeration, were monitored regarding their organics degradation rate with leachate recirculation. Hydrolysis reactions produced strong leachate in the column with no aeration. With forced aeration, the produced VFA could be effectively degraded, leading to the reduction in COD of the leachate effluent since the week 3. The CH_4 in the landfill gas from the column with aeration rate of 0.39 m3/(m3·d) and frequency of twice/d, leachate recirculation rate of 12.2 mm/d and frequency of twice/d, could amount to 40%(v/v) after only 20 weeks. This amount had increased up to 50% afterward even with no aeration. Most of COD in the recirculated leachate was removed. Using natural ventilation, CH_4 could also be produced and the COD of the leachate effluent be reduced after 10 weeks of operation. However, the persistent existence of oxygen in the landfill layer yielded instability in methanogenesis process.
基金the National Natural Science Foundation of China(No.51374035)the Foundation for the Author of National Excellent Doctoral Dissertation of PR China(No.201351)the Program for New Century Excellent Talents in University of China(No.NCET-13-0669).
文摘Despite lots of techniques in improving the heap leaching performance,many constraints on the industrial applications remain.We proposed a correspondingly effective and new idea of introducing forced aeration to improve the bad permeability and leaching effect of Yangla Copper Mine(YCM)during heap leaching.The dual-media theory was employed to study the impact mechanism of forced aeration on the variations of porous and fractured media during the column leaching experiments.An X-Ray Computed Tomography(CT)set was utilized to perform the pore imaging of the specimens and the fracture morphology of the particles within the columns was analyzed by Scanning Electron Microscope(SEM)as aeration rate(AR)changed.The results show that there exists copious fine particles within the heap of YCM,the particle size distribution of which is not reasonable.The forced aeration can not only promote the development of the porous and fractured structures but effectively break the blocked seepage paths.Then the leaching degree is improved and the seepage performance of the solute within the solution is enhanced.Therefore,the forced aeration is probable of making the leaching performance greatly improved.
基金Project supported by the National Natural Science Foundation of China (No. 50879023)the National Hi-Tech Research and Development Program (863) of China (No. 2007AA11Z135)the Min-istry of Water Resources Nonprofit Public Industry Special Foundation of China (No. 200701045)
文摘Due to large-scale dredging operations, a large amount of sludge is inevitably produced. Large areas of land are occupied when the dredged sludge is discarded in the disposal site as waste material. The sludge dewatering with aeration-vacuum (SDAV) method is suit for treating the sludge with high water content and high clay content in the disposal site. The water in the sludge can be discharged out. The volume of the sludge can be reduced quickly, and the recycling of the land can be accelerated by this method. Most importantly, this technique is an efficient way to deal with clogging problems when pumping water from high water content, high clay content dredged sludge. Vacuum degree range tests, the aeration rate range tests, and the influencing factors of sludge dewatering behavior tests were conducted with a self-developed SDAV model test device. Sludge samples were taken from the South-to-North Water Diversion East Line Project in Huai’an White-Horse Lake disposal site, Jiangsu Province, China. The optimal range of vacuum degree and aeration rate were obtained through the test results, and the mechanisms for how the two factors work and how they affect the sludge dewatering behavior were analyzed. The suitable vacuum degree range in SDAV is below 50 kPa, and the suitable aeration rate is about 1.0 m3/h. The low-vacuum degree contributes to reduce the ad-sorption effect of micro-channels on soil particles in filter material and to maintain the arch structures. Aeration has the effects of expansion, disturbance, changing Reynolds number, and dynamic sieve separating. The pump quantity of water per meter of filter tube (m) has different change rules as the vacuum degree changes under different aeration rates. The reason is that the formed arch structures’ conformation and permeability differ greatly under different combined-conditions of vacuum degree and aeration rate. The optimal combined-condition for dewatering the sludge is 35 kPa with 1.0 m3/h.
基金supported by the Public Projects of the Ministry of Environment Protection (No. 201209024)the State Key Laboratory of Urban Water Resource and Environment at Harbin Institute of Technology (No. 2015DX10)the Program for New Century Excellent Talents in University (No. NCET-12-0156)
文摘Pilot scale experiments with forced aeration were conducted to estimate effects of aeration rates on the performance of composting penicillin mycelial dreg using sewage sludge as inoculation. Three aeration rates of 0.15, 0.50 and 0.90 L/(min·kg) organic matter(OM) were examined. The principal physicochemical parameters were monitored during the 32 day composting period. Results showed that the higher aeration rate of 0.90 L/(min·kg) did not corresponded to a longer thermophilic duration and higher rates of OM degradation;but the lower aeration rate of 0.15 L/(min·kg) did induce an accumulation of NH+4-N contents due to the inhibition of nitrification. On the other hand, aeration rate has little effect on degradation of penicillin. The results show that the longest phase of thermophilic temperatures ≥ 55°C, the maximum NO-3-N content and seed germination, and the minimum C/N ratio were obtained with 0.50 L/(min·kg) OM. Therefore, aeration rates of0.50 L/(min·kg) OM can be recommended for composting penicillin mycelial dreg.
基金Supported by the Post-Doctoral Training Program from Research Affairs and Graduate School,Khon Kaen University(KKU).Thailand(Grant no.59153)
文摘To improve the efficiency of ethanol production in a batch fermentation from sweet sorghum juice under a very high gravity(VHG)condition(~290 g/L of total sugar)by Saccharomyces cerevisiae NP01,repeatedbatch fermentation under an aerated condition(2.5 vvm for the first 4 h during every cycle)was done in a5-L fermenter.The average ethanol concentration(P),productivity(Qp)and yield(Yp/s)for five successive cycles were 112.31 g/L,1.55 g/L·h^-1 and 0.44,respectively with 80.97%sugar consumption.To complete sugar consumption,the total sugar of the juice was reduced to a high gravity(HG)level(~240 g/L).The results showed that yeast extract was not necessary for ethanol production,and aeration during every other cycle i.e.,alternating cycles,was sufficient to promote both yeast growth and ethanol production.The average P,Qpand Yp/svalues for eight successive cycles with aeration during alternating cycles were97.58 g/L,1.98 g/Láh and 0.41,respectively with 91.21%sugar consumption.The total fatty acids in the yeast cells under the aerated condition were^50%higher than without aeration,irrespective the initial sugar concentration,whereas the ergosterol contents under aeration condition were^29%to 49%higher than those without aeration.
文摘Instream aeration has been used as a supplement to secondary treatment or a substitute for tertiary treatment for meeting dissolved oxygen (DO) standards in rivers. Many studies have used water quality models to determine the number, location, and capacity of instream aeration stations (IASs) needed to meet DO standards in combination with other pollution control measures. DO concentrations have been improved in the North Shore Channel and North Branch Chicago River by the Devon Avenue IAS for more than 35 years. A study was initiated to determine whether it was better to rehabilitate or relocate this station and to determine appropriate operational guidance for the IAS at the selected location. A water quality model capable of simulating DO concentrations during unsteady flow was used to evaluate the proper location for an IAS and operational guidance for this IAS. Three test years, a dry year, a wet year, and an extreme year, were considered in the evaluation. The study found that the Devon Avenue IAS should be rehabilitated as this location performed as well as or better than any of 10 alternative locations. According to the new operational guidance for this IAS, the amount of time with blowers operating could be substantially reduced compared to traditional operations while at the same time the attainment of the DO standards could be increased. This study shows that a carefully designed modeling study is key to effective selection, location, and operation of IASs such that attainment of DO standards can be maximized while operation hours of blowers can be minimized.
基金The authors thank York University and NSERC for providing funding and technical support
文摘Biological nutrient removal grows into complicated scenario due to the microbial consortium shift and kinetic competition between phosphorus(P)-accumulating and nitrogen(N)-removing microorganisms.In this study,three sequential batch reactors with constant operational conditions except aeration patterns at 6 h cycle periods were tested.Intermittent aeration was applied to develop a robust nutrient removal system aimed to achieve high energy saving and removal efficiency.The results showed higher correspondence of Puptake,polymeric substance synthesis and glycogen degradation in intermittent-aeration with longer interval periods compared to continuous-aeration.Increasing the intermittentaeration duration from 25 to 50 min,resulted in higher process performance where the system exhibited approximately 30%higher nutrient removal.This study indicated that nutrient removal strongly depends on reaction phase configuration representing the importance of aeration pattern.The microbial community examined the variation in abundance of bacterial groups in suspended sludge,where the 50 min intermittent aeration,favored the growth of P-accumulating organisms and nitrogen removalmicrobial groups,indicating the complications related to nutrient removal systems.Successful intermittently aerated process with high capability of simple implementation to conventional systems by elemental retrofitting,is applicable for upgrading wastewater treatment plants.With aeration as a major operational cost,this process is a promising approach to potentially remove nutrients in high competence,in distinction to optimizing cost-efficacy of the system.
基金supported by the National Natural Science Foundation of China (51309192)the National Key Research and Development Program of China (2016YFC0400201)the Fundamental Research Funds for the Central Universities, China (Z109021510)
文摘Aerated irrigation has been proven to increase crop production and quality, but studies on its environmental impacts are sparse. The effects of aeration and irrigation regimes on soil CO2 and N2O emissions in two consecutive greenhouse tomato rotation cycles in Northwest China were studied via the static closed chamber and gas chromatography technique. Four treatments, aerated deficit irrigation(AI1), non-aerated deficit irrigation(CK1), aerated full irrigation(AI2) and non-aerated full irrigation(CK2), were performed. The results showed that the tomato yield under aeration of each irrigation regime increased by 18.8% on average compared to non-aeration, and the difference was significant under full irrigation(P〈0.05). Full irrigation significantly increased the tomato yield by 23.9% on average in comparison to deficit irrigation. Moreover, aeration increased the cumulative CO2 emissions compared to non-aeration, and treatment effects were significant in the autumn-winter season(P〈0.05). A slight increase of CO2 emissions in the two seasons was observed under full irrigation(P〉0.05). There was no significant difference between aeration and non-aeration in soil N2O emissions in the spring-summer season, whereas aeration enhanced N2O emissions significantly in the autumn-winter season. Furthermore, full irrigation over the two seasons greatly increased soil N2O emissions compared to the deficit irrigation treatment(P〈0.05). Correlation analysis indicated that soil temperature was the primary factor influencing CO2 fluxes. Soil temperature, soil moisture and NO3^- were the primary factors influencing N2O fluxes. Irrigation coupled with particular soil aeration practices may allow for a balance between crop production yield and greenhouse gas mitigation in greenhouse vegetable fields.
基金Supported by the Key Science Research Project of the Eleventh Five-Year Plan (2008ZX07106-003, 2008ZX07316-004)Tianjin Binhai New Area’s Construction Science and Technology Action Planning Project of Chinese Academy of Scienses (TJZX2-YW-07)
文摘In order to improve removal for nitrogen in a pilot-scale submerged membrane bioreactor(SMBR),intermittent aeration was conducted,and the effect on the treatment performance under four kinds of operation condition(run 1,continuous aeration;run 2,60/60 min aeration on/off time;run 3,60/90 min aeration on/off time;run 4,60/75 min aeration on/off time) was evaluated.The results showed that depending on the specific on/off of the aeration time ratio,removal efficiency of nitrogen could be improved significantly,and the removal rates of total nitrogen(TN) under different operation conditions were 28.0%,59.5%,66.8% and 70.7%,respectively.There were no obvious differences for removal rates for CODCr and ammonia among different operation conditions.In general,intermittent aeration could be used as a feasible way to improve treatment performance for nitrogen in the SMBR.
文摘Free surface flows aeration potential causing the in macro- and intermediate flow characteristics to vary roughness conditions have a high with slopes and discharges. The underlying mechanism of two-phase flow characteristics in macro- and intermediate roughness conditions were analyzed in an experimental setup assembled at the Laboratory of Hydraulic Protection of the Territory (PITLAB) of the University of Pisa, Italy. Crushed angular rocks and hemispherical boulders were used to intensify the roughness of the bed. Flow rates per unit width ranging between 0.03 m^2/s and 0.09 m^2/s and slopes between 0.26 and 0.46 were tested over different arrangements of a rough bed. Analyses were mainly carried out in the inner flow region, which consists of both bubbly and intermediate flow regions. The findings revealed that the two-phase flow properties over the rough bed were much affected by rough bed arrangements. Turbulence features of two-phase flows over the rough bed were compared with those of the stepped chute data under similar flow conditions. Overall, the results highlight the flow features in the inner layers of the two-phase flow, showing that the maximum turbulence intensity decreases with the relative submergence, while the bubble frequency distribution is affected by the rough bed elements.
基金supported by the Major Water Project of the National Science and Technology (No.2011ZX07319-001-004, 2011ZX07301-002)
文摘Knowledge of the oxygen mass transfer of aerators under operational conditions in a full-scale wastewater treatment plant (WWTP) is meaningful for the optimization of WWTP, however, scarce to best of our knowledge. Through analyzing a plug flow aeration tank in the Lucun WWTP, in Wuxi, China, the oxygenation capacity of fine-bubble aerators under process conditions have been measured in- situ using the off-gas method and the non-steady-state method. The off-gas method demonstrated that the aerators in different corridors in the aeration tank of WWTP had significantly different oxygen transfer performance; furthermore, the aerators in the same corridor shared almost equal oxygen transfer performance over the course of a day. Results measured by the two methods showed that the oxygen transfer performance of fine-bubble aerators in the aeration tank decreased dramatically compared with that in the clean water. The loss of oxygen transfer coefficient was over 50% under low-aeration conditions (aeration amount 〈 0.67 Nm 3 /hr). However, as the aeration amount reached 0.96 Nm 3 /hr, the discrepancy of oxygen transfer between the process condition and clean water was negligible. The analysis also indicated that the non-steady-state and off-gas methods resulted in comparable estimates of oxygen transfer parameters for the aerators under process conditions.
基金the financial support by the National Natural Science Foundation of China(52230004 and 52293445)the Key Research and Development Project of Shandong Province(2020CXGC011202-005)the Shenzhen Science and Technology Program(KCXFZ20211020163404007 and KQTD20190929172630447).
文摘The potential for reducing greenhouse gas(GHG)emissions and energy consumption in wastewater treatment can be realized through intelligent control,with machine learning(ML)and multimodality emerging as a promising solution.Here,we introduce an ML technique based on multimodal strategies,focusing specifically on intelligent aeration control in wastewater treatment plants(WWTPs).The generalization of the multimodal strategy is demonstrated on eight ML models.The results demonstrate that this multimodal strategy significantly enhances model indicators for ML in environmental science and the efficiency of aeration control,exhibiting exceptional performance and interpretability.Integrating random forest with visual models achieves the highest accuracy in forecasting aeration quantity in multimodal models,with a mean absolute percentage error of 4.4%and a coefficient of determination of 0.948.Practical testing in a full-scale plant reveals that the multimodal model can reduce operation costs by 19.8%compared to traditional fuzzy control methods.The potential application of these strategies in critical water science domains is discussed.To foster accessibility and promote widespread adoption,the multimodal ML models are freely available on GitHub,thereby eliminating technical barriers and encouraging the application of artificial intelligence in urban wastewater treatment.
基金supported by the National Key Research and Development Plan of China(Grant No.2017YFD0801102)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.2017RG006-5)+1 种基金the National Science Foundation of China(Grant No.31701407)the Chinese Academy of Agricultural Sciences to the Scientific and Technical Innovation Team.
文摘Different cadmium(Cd)-accumulated rice genotypes(Erjiunan 1 and Fupin 36)were used to explore the effect of rice rhizosphere aeration on Cd uptake and accumulation.Aeration in the nutrient soluti on influe need the agronomic characteristics in duced by Cd-stress,such as the in creases of rice root length and root vigor,but the reductions of plant height and shoot dry weight.Aeration also alleviated the decreasing effects of Cd stress on antioxidant enzyme activities and soluble protein,malonaldehyde and nicotianamine contents in rice.Moreover,with aeration treatment,the accumulation and bioavailability of metal elements changed significantly,with a Cd increase and an Fe reduction in both rice genotypes.In addition,at the molecular level,aeration upregulated the expression of Fe-inducible genes(such as OsIRTI,OsNRAMPI,OsYSL15 and OsNAS3).Furthermore,as a Cd^(2+)/Fe^(2+) transporter,the high transcription level of OsNRAMPI can elevate the Cd uptake and translocation in rice due to the Fe reduction caused by aeration and Cd-exposure,which indicated that OsNRAMPI might play a crucial role in the effect of aeration on Cd uptake and accumulation.