Platelet indices(PIs)including high mean platelet volume(MPV),plateletcrit(PLC),and platelet distribution width(PLDW)are associated with poor glycemic control.In addition,they can indicate prothrombotic and procoagula...Platelet indices(PIs)including high mean platelet volume(MPV),plateletcrit(PLC),and platelet distribution width(PLDW)are associated with poor glycemic control.In addition,they can indicate prothrombotic and procoagulation risk among patients with diabetes.PI measurement is cheap,quick and fits healthcare system needs in remote outreaching areas in low-income countries.However,a broader insight into their clinical implications in diabetes is lacking.To achieve a wider understanding,we reviewed PubMed/MEDLINE,Google Scholar and Cochrane Library for relevant articles investigating the role of PIs in diabetes mellitus.No limitation to the publication date was applied,which included all articles published up to August 17,2024.The terms used were MPV,PLC,PLDW,platelet large cell ratio,glycated hemoglobin(HbA1c),PIs,platelet activity and diabetes mellitus.Out of the 790 articles retrieved,187 full texts were reviewed,and 44 were included.PIs,when measurements are done promptly and within 2 h,could be short-term pointers to glycemic control in the life span of the platelets(2 wk).PIs are easy to perform,cheap and useful in remote outreaching areas with limited facilities where measurement of HbA1c is not available or cost-effective.However,PIs are not specific and are affected by demographic factors,such as pregnancy,renal failure,medications,hemoglobin and duration of diabetes.PIs could be implemented with daily blood glucose to inform doctors in low-income countries about their patients'glycemic control and cardiovascular risk.An important application might be when blood glucose control is needed quickly(before elective surgery).展开更多
Crimean-Congo hemorrhagic fever(CCHF)is a hemorrhagic fever caused by infection with the CCHF virus(CCHFV)and has a mortality rate of up to 30%.Thrombocytopenia is a hallmark of CCHF;however,the mechanisms underlying ...Crimean-Congo hemorrhagic fever(CCHF)is a hemorrhagic fever caused by infection with the CCHF virus(CCHFV)and has a mortality rate of up to 30%.Thrombocytopenia is a hallmark of CCHF;however,the mechanisms underlying this manifestation remain poorly understood.In addition to hemostasis,platelets play a crucial role in recognizing pathogens and mediating immune responses.We investigated the mechanisms underlying thrombocytopenia associated with CCHFV infection by analyzing the platelet transcriptome in mice.Interferon-induced transmembrane protein 3(IFITM3),a known antiviral factor,was significantly upregulated.The role of IFITM3 in response to CCHFV infection was characterized using the human megakaryoblast cell line MEG-01,considered a parental cell line of platelets.Although the CCHFV infection rate was limited,MEG-01 cells maintained the infection and replication of CCHFV,leading to increased IFITM3 protein expression.We demonstrated that IFITM3 overexpression efficiently inhibited CCHFV infection,whereas IFITM3 knockout promoted viral infection.An interaction between IFITM3 and the CCHFV glycoprotein Gc was identified,which suppressed CCHFV entry into cells.The IFITM3 CIL-TMD domain is critical for this interaction.These results suggest that IFITM3 is a restriction factor and plays an antiviral role during CCHFV infection.Elevated expression of IFITM3 in platelets indicates that this could be a common mechanism by which platelets protect against viruses,including CCHFV,which may reduce platelet consumption and destruction caused by CCHFV infection.These findings provide valuable insights into the pathogenesis of CCHF-associated thrombocytopenia and offer foundational theoretical support for future therapeutic strategies.展开更多
目的探讨慢性乙型肝炎病毒(hepatitis B virus,HBV)感染相关肝病患者血清壳多糖酶3样蛋白1(chitosan 3-like protein 1,CHI3L1)水平与γ-谷氨酰转肽酶/血小板比值(GPR)和天冬氨酸氨基转移酶/血小板比值(APRI)的相关性及诊断价值。方法...目的探讨慢性乙型肝炎病毒(hepatitis B virus,HBV)感染相关肝病患者血清壳多糖酶3样蛋白1(chitosan 3-like protein 1,CHI3L1)水平与γ-谷氨酰转肽酶/血小板比值(GPR)和天冬氨酸氨基转移酶/血小板比值(APRI)的相关性及诊断价值。方法将纳入的慢性HBV感染者221例,分为慢性乙型肝炎(CHB,n=49)、肝硬化(LC,n=132)和原发性肝癌(PHC,n=40)3组。分别检测血清CHI3L1、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷氨酰转肽酶(γ-GGT)和血小板(PLT),计算GPR及APRI。结果①PHC组年龄、CHI3L1、γ-GGT、GPR及APRI显著高于CHB组(P<0.05),ALT、PLT显著降低(P<0.05);PHC组AST、γ-GGT、GPR及APRI显著高于LC组(P<0.05)。②CHI3L1诊断CHB、LC和PHC的AUC分别为0.748(95%CI:0.640~0.856)、0.933(95%CI:0.896~0.971)和0.928(95%CI:0.860~0.997);GPR诊断CHB、LC和PHC的AUC分别为0.852(95%CI:0.767~0.938)、0.945(95%CI:0.912~0.978)和0.992(95%CI:0.980~1.000);APRI诊断CH组、LC和PHC的AUC分别为0.665(9%CI:0.543~0.786)、0.737(95%CI:0.661~0.813)和0.893(95%CI:0.811~0.974)。③Spearman相关性分析表明,3组患者CHI3L1与GPR、APRI均呈正相关(P<0.05)。结论CHB组、LC组和PHC组中的CHI3L1与GPR的相关性明显优于CHI3L1与APRI的相关性,可用GPR来替代CHI3L1预测肝脏的病变程度。展开更多
文摘Platelet indices(PIs)including high mean platelet volume(MPV),plateletcrit(PLC),and platelet distribution width(PLDW)are associated with poor glycemic control.In addition,they can indicate prothrombotic and procoagulation risk among patients with diabetes.PI measurement is cheap,quick and fits healthcare system needs in remote outreaching areas in low-income countries.However,a broader insight into their clinical implications in diabetes is lacking.To achieve a wider understanding,we reviewed PubMed/MEDLINE,Google Scholar and Cochrane Library for relevant articles investigating the role of PIs in diabetes mellitus.No limitation to the publication date was applied,which included all articles published up to August 17,2024.The terms used were MPV,PLC,PLDW,platelet large cell ratio,glycated hemoglobin(HbA1c),PIs,platelet activity and diabetes mellitus.Out of the 790 articles retrieved,187 full texts were reviewed,and 44 were included.PIs,when measurements are done promptly and within 2 h,could be short-term pointers to glycemic control in the life span of the platelets(2 wk).PIs are easy to perform,cheap and useful in remote outreaching areas with limited facilities where measurement of HbA1c is not available or cost-effective.However,PIs are not specific and are affected by demographic factors,such as pregnancy,renal failure,medications,hemoglobin and duration of diabetes.PIs could be implemented with daily blood glucose to inform doctors in low-income countries about their patients'glycemic control and cardiovascular risk.An important application might be when blood glucose control is needed quickly(before elective surgery).
基金supported by the the National Key R&D Program of China(2024YFC2310000)the Key Project of Key Laboratory of VirologyBiosafety in the Wuhan Institute of Virology,Chinese Academy of Sciences(2024JZZD-02),the Youth Project of the Wuhan Institute of Virology,Chinese Academy of Sciences(2023QNTJ-03)+2 种基金the"Open Competition for Selecting the Best Candidates"Project of the Wuhan East Lake New Technology Development Zone(2022KJB117)the Natural Science Foundation of Hubei Province(2024AFB986)the Medical Science Research Project of Wuhan Health Commission(WX23B09).
文摘Crimean-Congo hemorrhagic fever(CCHF)is a hemorrhagic fever caused by infection with the CCHF virus(CCHFV)and has a mortality rate of up to 30%.Thrombocytopenia is a hallmark of CCHF;however,the mechanisms underlying this manifestation remain poorly understood.In addition to hemostasis,platelets play a crucial role in recognizing pathogens and mediating immune responses.We investigated the mechanisms underlying thrombocytopenia associated with CCHFV infection by analyzing the platelet transcriptome in mice.Interferon-induced transmembrane protein 3(IFITM3),a known antiviral factor,was significantly upregulated.The role of IFITM3 in response to CCHFV infection was characterized using the human megakaryoblast cell line MEG-01,considered a parental cell line of platelets.Although the CCHFV infection rate was limited,MEG-01 cells maintained the infection and replication of CCHFV,leading to increased IFITM3 protein expression.We demonstrated that IFITM3 overexpression efficiently inhibited CCHFV infection,whereas IFITM3 knockout promoted viral infection.An interaction between IFITM3 and the CCHFV glycoprotein Gc was identified,which suppressed CCHFV entry into cells.The IFITM3 CIL-TMD domain is critical for this interaction.These results suggest that IFITM3 is a restriction factor and plays an antiviral role during CCHFV infection.Elevated expression of IFITM3 in platelets indicates that this could be a common mechanism by which platelets protect against viruses,including CCHFV,which may reduce platelet consumption and destruction caused by CCHFV infection.These findings provide valuable insights into the pathogenesis of CCHF-associated thrombocytopenia and offer foundational theoretical support for future therapeutic strategies.
文摘目的探讨慢性乙型肝炎病毒(hepatitis B virus,HBV)感染相关肝病患者血清壳多糖酶3样蛋白1(chitosan 3-like protein 1,CHI3L1)水平与γ-谷氨酰转肽酶/血小板比值(GPR)和天冬氨酸氨基转移酶/血小板比值(APRI)的相关性及诊断价值。方法将纳入的慢性HBV感染者221例,分为慢性乙型肝炎(CHB,n=49)、肝硬化(LC,n=132)和原发性肝癌(PHC,n=40)3组。分别检测血清CHI3L1、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷氨酰转肽酶(γ-GGT)和血小板(PLT),计算GPR及APRI。结果①PHC组年龄、CHI3L1、γ-GGT、GPR及APRI显著高于CHB组(P<0.05),ALT、PLT显著降低(P<0.05);PHC组AST、γ-GGT、GPR及APRI显著高于LC组(P<0.05)。②CHI3L1诊断CHB、LC和PHC的AUC分别为0.748(95%CI:0.640~0.856)、0.933(95%CI:0.896~0.971)和0.928(95%CI:0.860~0.997);GPR诊断CHB、LC和PHC的AUC分别为0.852(95%CI:0.767~0.938)、0.945(95%CI:0.912~0.978)和0.992(95%CI:0.980~1.000);APRI诊断CH组、LC和PHC的AUC分别为0.665(9%CI:0.543~0.786)、0.737(95%CI:0.661~0.813)和0.893(95%CI:0.811~0.974)。③Spearman相关性分析表明,3组患者CHI3L1与GPR、APRI均呈正相关(P<0.05)。结论CHB组、LC组和PHC组中的CHI3L1与GPR的相关性明显优于CHI3L1与APRI的相关性,可用GPR来替代CHI3L1预测肝脏的病变程度。
