AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to ...AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury;nonlethal sepsis induced in rats by single puncture cecal ligation and puncture(CLP)was used as the other model to evaluate the specificity of plasma miR-216a compared with twocommonly used markers(amylase and lipase)for acute pancreatitis.Plasmas were sampled from rats at indicated time points and total RNA was isolated.Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.RESULTS:In the acute pancreatitis model,among five time points at which plasmas were sampled,miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation(compared with 0 h time point,P<0.01,Kruskal-Wallis Test).In the CLP model,plasma amylase and lipase,two commonly used biomarkers for acute pancreatitis,were significantly elevated 24 h after operation(compared with 0 h time point,P<0.01 and 0.05 respectively,Pairwise Bonferroni corrected ttests),while miR-216a remained undetectable among four tested time points.CONCLUSION:Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity.展开更多
目的研究利用miRNA-1诊断急性心肌梗死,在分子生物学层面拓展急性心肌梗死法医病理学诊断指标方法通过大鼠A M I动物模型,利用实时定量P C R技术检测急性心梗发生后血浆m iRN A-1表达变化水平,并与免疫组化指标Connexin 43进行对比;利...目的研究利用miRNA-1诊断急性心肌梗死,在分子生物学层面拓展急性心肌梗死法医病理学诊断指标方法通过大鼠A M I动物模型,利用实时定量P C R技术检测急性心梗发生后血浆m iRN A-1表达变化水平,并与免疫组化指标Connexin 43进行对比;利用实时定量P C R技术检测死亡早期m iR N A-1变化趋势结果A M I发生30min,血浆m iR N A-1含量开始升高,A M I发生丨h、2 h后,血浆中m iR N A-1相对含量平均值分别是对照组的3倍、5倍;而免疫组化指标Comiexin 43在AM丨发生30m in后的表达部位发生改变,表达量升高;在死后稳定性方面,大鼠死亡Oh、3h、6h、12h后,血浆miRNA-1含量没有统计学差异(p>0.05),但在24 h后,血浆miRNA-1明显升高,其平均值约为Oh组的42倍(p<0.05)。结论在心梗发生早期,血浆m iR N A-1含量特异性升高,并能在死亡早期保持稳定,有望作为法医病理诊断急性心肌梗死辅助分子指标。展开更多
[目的]探讨血浆miRNAs水平检测在原发性肝细胞癌早期筛查中的作用。[方法]采用Taq Man低密度芯片(Taq Man Low Density Array)的方法,筛选出候选可作为原发性肝细胞癌早期筛查的miRNAs标志物,并采用RT-qPCR方法在98例原发性肝细胞癌样...[目的]探讨血浆miRNAs水平检测在原发性肝细胞癌早期筛查中的作用。[方法]采用Taq Man低密度芯片(Taq Man Low Density Array)的方法,筛选出候选可作为原发性肝细胞癌早期筛查的miRNAs标志物,并采用RT-qPCR方法在98例原发性肝细胞癌样本中进行验证。[结果]5个候选miRNAs在HCC患者血浆中的表达与对照组相比显差异有统计学意义,其中miR-21(病例组ΔCt=5.8675±1.3561,对照组ΔCt=8.5214±1.8956)、miR-222(病例组ΔCt=6.2568±2.5842,对照组ΔCt=9.2354±1.5627)、miR-18(病例组ΔCt=9.4237±2.4785,对照组ΔCt=12.5862±0.9852)在病例组中的表达量高于对照组,miR-16(病例组ΔCt=13.4211±1.2258,对照组ΔCt=8.4251±0.8625)、miR-451a(病例组ΔCt=8.2358±1.2372,对照组ΔCt=5.2666±1.1656)在病例组中的表达量低于对照组;miR-21在对照组、Ⅰ期、Ⅱ期、Ⅲ期及以上组中表达量存在差异性,呈现递增趋势(P<0.05),miR-16在对照组、Ⅰ期、Ⅱ期、Ⅲ期及以上组中表达量存在差异性,呈现递减趋势(P<0.05)。[结论]患者血浆miRNAs表达的差异可能可作为原发性肝细胞癌早期筛查的血浆学分子标志物。展开更多
基金Supported by National Nature Science Foundation of China,No.30971344Innovative Fund for PhD granted by the Second Military Medical University
文摘AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury;nonlethal sepsis induced in rats by single puncture cecal ligation and puncture(CLP)was used as the other model to evaluate the specificity of plasma miR-216a compared with twocommonly used markers(amylase and lipase)for acute pancreatitis.Plasmas were sampled from rats at indicated time points and total RNA was isolated.Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.RESULTS:In the acute pancreatitis model,among five time points at which plasmas were sampled,miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation(compared with 0 h time point,P<0.01,Kruskal-Wallis Test).In the CLP model,plasma amylase and lipase,two commonly used biomarkers for acute pancreatitis,were significantly elevated 24 h after operation(compared with 0 h time point,P<0.01 and 0.05 respectively,Pairwise Bonferroni corrected ttests),while miR-216a remained undetectable among four tested time points.CONCLUSION:Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity.
文摘目的研究利用miRNA-1诊断急性心肌梗死,在分子生物学层面拓展急性心肌梗死法医病理学诊断指标方法通过大鼠A M I动物模型,利用实时定量P C R技术检测急性心梗发生后血浆m iRN A-1表达变化水平,并与免疫组化指标Connexin 43进行对比;利用实时定量P C R技术检测死亡早期m iR N A-1变化趋势结果A M I发生30min,血浆m iR N A-1含量开始升高,A M I发生丨h、2 h后,血浆中m iR N A-1相对含量平均值分别是对照组的3倍、5倍;而免疫组化指标Comiexin 43在AM丨发生30m in后的表达部位发生改变,表达量升高;在死后稳定性方面,大鼠死亡Oh、3h、6h、12h后,血浆miRNA-1含量没有统计学差异(p>0.05),但在24 h后,血浆miRNA-1明显升高,其平均值约为Oh组的42倍(p<0.05)。结论在心梗发生早期,血浆m iR N A-1含量特异性升高,并能在死亡早期保持稳定,有望作为法医病理诊断急性心肌梗死辅助分子指标。
文摘[目的]探讨血浆miRNAs水平检测在原发性肝细胞癌早期筛查中的作用。[方法]采用Taq Man低密度芯片(Taq Man Low Density Array)的方法,筛选出候选可作为原发性肝细胞癌早期筛查的miRNAs标志物,并采用RT-qPCR方法在98例原发性肝细胞癌样本中进行验证。[结果]5个候选miRNAs在HCC患者血浆中的表达与对照组相比显差异有统计学意义,其中miR-21(病例组ΔCt=5.8675±1.3561,对照组ΔCt=8.5214±1.8956)、miR-222(病例组ΔCt=6.2568±2.5842,对照组ΔCt=9.2354±1.5627)、miR-18(病例组ΔCt=9.4237±2.4785,对照组ΔCt=12.5862±0.9852)在病例组中的表达量高于对照组,miR-16(病例组ΔCt=13.4211±1.2258,对照组ΔCt=8.4251±0.8625)、miR-451a(病例组ΔCt=8.2358±1.2372,对照组ΔCt=5.2666±1.1656)在病例组中的表达量低于对照组;miR-21在对照组、Ⅰ期、Ⅱ期、Ⅲ期及以上组中表达量存在差异性,呈现递增趋势(P<0.05),miR-16在对照组、Ⅰ期、Ⅱ期、Ⅲ期及以上组中表达量存在差异性,呈现递减趋势(P<0.05)。[结论]患者血浆miRNAs表达的差异可能可作为原发性肝细胞癌早期筛查的血浆学分子标志物。
