The cell fate of primordial germ cell(PGC)in zebrafish is pre-determined by maternally deposited germ plasm,which is packaged into ribonucleoprotein complex in oocytes and inherited into PGC-fated cells in embryos.How...The cell fate of primordial germ cell(PGC)in zebrafish is pre-determined by maternally deposited germ plasm,which is packaged into ribonucleoprotein complex in oocytes and inherited into PGC-fated cells in embryos.However,the maternal factors regulating the assembly of germ plasm and PGC development remain poorly understood.In this study,we report that the maternal transcription factor Znf706 regulates the assembly of germ plasm factors into a granule-like structure localized perinuclearly in PGC during migration.Maternal and zygotic mutants of znf706 exhibit deficient germ plasm scattering at the early embryonic stage,decreased PGC numbers with some mislocation during PGC migration,and a lower female ratio in adulthood.Notably,the implementation of Znf706 CUT&Tag and RNA-seq on immature oocytes uncovers that Znf706 in stage I oocytes may promote transcription of several mitochondrial genes in addition to other functions.Hence,we propose that Znf706 is implicated in germ plasm assembly and PGC development in zebrafish.展开更多
The dual-frequency capacitively coupled plasma （DF-CCP） with inductive enhancement system is a newly designed plasma reactor. Different from the conventional inductively coupled plasma （ICP） reactors, now a radio ...The dual-frequency capacitively coupled plasma （DF-CCP） with inductive enhancement system is a newly designed plasma reactor. Different from the conventional inductively coupled plasma （ICP） reactors, now a radio frequency （rf） power is connected to an antenna placed outside the chamber with a one-turn bare coil placed between two electrodes in DF-CCP. This paper gives a detailed description of its structure of discharges in this apparatus were made via a Moreover, investigations on some characteristics Langmuir probe.展开更多
We report a feasible method to realize tun able surface plasm on-polarit on(SPP)res onance in orga nic light-emitt ing devices(OLEDs)by emplo ying corrugated Ag-Al alloy electrodes.The excited SPP res onance in duced ...We report a feasible method to realize tun able surface plasm on-polarit on(SPP)res onance in orga nic light-emitt ing devices(OLEDs)by emplo ying corrugated Ag-Al alloy electrodes.The excited SPP res onance in duced by the periodic corrugations can be precisely tuned based on the composition ratios of the Ag-Al alloy electrodes.With an appropriate composition ratio of the corrugated alloy electrode,the photons trapped in SPP modes are recovered and extracted effectively.The 25%in creaseme nt in luminance and 21%enhan ceme nt in curre nt efficie ncy have bee n achieved by using the corrugated Ag-Al alloy electrodes in OLEDs.展开更多
The stability of stratified of incompressible, viscoelastic plasma through a porous medium in the presence of the quantum mechanism is considered. The dispersion relation is obtained using the normal mode technique. T...The stability of stratified of incompressible, viscoelastic plasma through a porous medium in the presence of the quantum mechanism is considered. The dispersion relation is obtained using the normal mode technique. The behavior of growth rate with respect to the quantum effect, strain retardation time and stress relaxation time are examined in the presence of porosity of the porous medium, the medium permeability, kinematic viscosity. It is shown that, the presence of quantum term stabilizes a certain wave number band, whereas the system is unstable for all wave numbers in the absence of quantum term. The considered parameters beside the quantum term will bring about more stability on the considered system.展开更多
BACKGROUND： Inflammatory reaction and the increased level of its accompanying active protein play an important role in the occurrence and development of cerebral infarction. C-reactive protein, fibrinogen and white b...BACKGROUND： Inflammatory reaction and the increased level of its accompanying active protein play an important role in the occurrence and development of cerebral infarction. C-reactive protein, fibrinogen and white blood cell, as the monitoring index of inflammatory reaction, are very important in the occurrence and development of acute cerebral infarction. OBJECTIVE： To make a comparison between patients with primary hypertension accompanied with acute cerebral infarction and with simple primary hypertension by observing the changes in plasma C-reactive protein and fibrinogen levels as well as white blood cell and differential counts and analyzing their significances. DESIGN ： Controlled observation SETTING ： Ward Building for VIP, Shenzhen Hospital, Peking University. PARTICIPANTS： Totally 133 patients with primary hypertension were selected from Ward Building for VIP, Shenzhen Hospital, Peking University during September 2003 to September 2005, The diagnostic criteria were based on the hypertension diagnosis criteria formulated by the 7^th World Health Organization-International Society of Hypertension Guidelines （WHO-ISH） in 1998. The informed consents were obtained from all the participants. The involved patients were assigned into two groups： primary hypertension group, in which, there were 65 patients with primary hypertension （ degree 2）, including 42 males and 23 females, with mean age of （61 ±14）years and mean blood pressure of （162.7±6.8）/（94.2±8.4） mm Hg （1 mm Hg =0.133 kPa）, and primary hypertension combined with cerebral infarction group, in which, there were 68 patients with primary hypertension combined with cerebral infarction （ meeting the diagnostic criteria formulated in the 4^th National Cerebrovascular Diseases Meeting in 1995 and diagnosed by skull CT or MRI to exclude the patients with lacunar infarction）, including 42 males and 26 females, with mean age of （56±15） years and mean blood pressure of （176.4±9.2）/（96.3±9.7） mm Hg. METHODS： Plasm C-reactive protein and fibrinogen levels, and white blood cell and differential counts of patients in the two groups were examined 24 hours after stroke. The above indexes were re-examined in the primary hypertension combined with cerebral infarction group 72 hours after stroke. White blood cell and differential counts were performed with laser method （East Asia FE-95001 RAM-1, Japan）. The level of C-reactive protein was measured with turbidimetry （BNII Automatic Systems For Analysis, USA）. The level of fibrinogen was measured with algorithm method when prothrombin time was normal and with Clauss method when prothrombin time was abnormal （ACL Automatic Coagulation Analyzer, USA）. MAIN OUTCOME MEASURES： The plasm C-reactive protein and flbrinogen levels, and white blood cell and differential counts 24 hours after stroke in two groups and 72 hours after stroke in primary hypertension combined with cerebral infarction group. RESULTS： All the 133 involved patients participated in the result analysis. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neutrophil counts in patients with primary hypertension were all within the normal range. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neu- trophil counts in patients with primary hypertension combined with cerebral infarction were significantly higher than those in patients with primary hypertension 24 hours after stroke and 72 hours after stroke respectively[24 hours after stroke：（32.12±11.76） mg/L vs. （5.02±3.21 ） mg/L;（4.64±0.75） g/L vs. （3.12±0.49） g/L; （9.32±81）×10^9 L^- 1 vs. （5.78±1.32）×10^9L^- 1 （7.85±2.38）×10^9 L^- 1 vs.（3.49±1.28）×10^9 L^-1,t =7.094, 5.759,4.106,5.491, respectively,all P〈 0.01; 72 hours after stroke： （47.62±18.43） mg/L vs. （32.12±11.76） mg/L; （5.08±0.82） g/L vs. （4.64±0.75） g/L, t =2.864,2.220, respectively, both P 〈 0.05]. CONCLUSION： The increase in fibrinogen level and white blood cell count are the important index in monitoring primary hypertension combined with acute cerebral infarction. The increase in plasm C-reactive protein and fibrinogen levels 72 hours after stroke indicates that plasma C-reactive protein and fibrinogen are very important in the development of disease.展开更多
2D-material-based photodetectors enhanced by plasmonic nanostructures can support responsivity/detectivity several orders higher than commercial photodetectors,drawing extensive attention as promising candidates for t...2D-material-based photodetectors enhanced by plasmonic nanostructures can support responsivity/detectivity several orders higher than commercial photodetectors,drawing extensive attention as promising candidates for the next-generation photodetectors.However,to boost the nanostructure-enhanced 2D photodetectors into real-world applications,crucial challenges lie in the design of broadband enhancing nanostructures and their scalable and position-controllable fabrication.Here,based on a broadband resonant plasmonic disk array fabricated by a scalable and position-controllable technique(direct writing photolithography),we present a visible-near infrared(405-1310 nm)2D WS_(2) photodetector,whose detectivity is up to 3.9×10^(14)Jones,a value exceeding that of the previous plasmon-enhanced 2D photodetectors.The broadened spectral response range and the high detectivity originate from the hot electron injection,optical absorption enhancement,and strain effect supported by the plasmonic array.Furthermore,the designed plasmonic 2D photodetector supports self-powered photodetection,indicating promising potential in energy-free and portable optoelectronic systems.Our results demonstrate an effective method to construct high-performance broadband photodetectors,which can facilitate the development of 2D photodetectors in commercial applications.展开更多
When quantum emitters and plasmonic nanoparticles are in close vicinity,the energy exchange,termed as plasmon-exciton coupling,can make the absorption and emission behavior of the hybrid structure very different from ...When quantum emitters and plasmonic nanoparticles are in close vicinity,the energy exchange,termed as plasmon-exciton coupling,can make the absorption and emission behavior of the hybrid structure very different from those of the two constituents alone.The coupling strength between the two constituents highly depends on how the hybrid structure is constructed.As a result,a diverse range of coupling effect arise including plasmon induced fluorescence quenching/enhancing (weak coupling),Fano interference (intermediate coupling),Rabi-splitting and lasing (strong coupling).The emergence of different coupling behavior can be controlled by the different combinations of quantum emitters and plasmonic nanoparticles as well as the spatial arrangement of the individual components.Colloidal assembly/synthesis methods are essentially delicate strategies that can build the hybrid nanostructures with nanometer precision and allow for large-scale processing.In this review,we discuss the theoretical models that apply to different coupling behaviors,the optical properties of the hybrid systems,and the advancement of colloidal methods to manipulate the plasmon-exciton in the hybrid structures.We also provide perspectives on the challenges and future directions of the research in coupled plasmon-exciton nanosystems.展开更多
Bucky ball(Buc)is involved in germ plasm(GP)assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m^(6)A reader Igf2bp3 interacts ...Bucky ball(Buc)is involved in germ plasm(GP)assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m^(6)A reader Igf2bp3 interacts and colocalizes with Buc in the GP.Similar to the loss of Buc,the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification,which can be partially restored by the injection of igf2 bp3 mRNA.Igf2bp3 binds to m^(6)A-modified GPorganizer and GP mRNAs in an m^(6)A-dependent manner and prevents their degradation.These findings indicate that the functions of Igf2bp3,a direct effector protein of Buc,in GP mRNA expression and GP assembly involve m^(6)A-dependent regulation;these results emphasize a critical role of m^(6)A modification in the process of GP assembly.展开更多
Gangliocytic paraganglioma (GP) is rare and has been regarded as benign in general with a good prognosis. We present a patient with duodenal GP showing a malignant and lethal clinical course. A 47-year-old male patien...Gangliocytic paraganglioma (GP) is rare and has been regarded as benign in general with a good prognosis. We present a patient with duodenal GP showing a malignant and lethal clinical course. A 47-year-old male patient was found to have a duodenal tumor and enlarged regional lymph nodes. The patient initially underwent a pancreaticoduodenectomy to resect the tumor and involved lymph nodes completely. Histological and immunohistochemical analyses showed findings typical of GP. However, the distant metastatic lesions in the liver and pelvic cavity were rapidly observed after surgery. The patient underwent chemotherapy and radiotherapy, as well as a second surgery to partly remove the metastatic mass in the pelvic cavity. The histological examination revealed no significant difference in histological features between the primary duodenal tumor and the metastatic pelvic mass. However, the patient finally died of the tumor due to the recurrence of the residual pelvic lesion and increased liver mass. To our knowledge, this is the first report of lethal GP with multifocal metastases. Our case confirms that GP should be regarded as a malignant potential tumor with behavior code of “1”, rather than a benign tumor of “0”.展开更多
基金supported by the National Natural Science Foundation of China(31988101 to A.M.)the National Key Research and Development Program of China(2023YFA1800300 to X.W.and 2018YFC1003304 to A.M.)the Yunnan Provincial Science and Technology Project at Southwest United Graduate School(202302A0370011 to A.M.).
文摘The cell fate of primordial germ cell(PGC)in zebrafish is pre-determined by maternally deposited germ plasm,which is packaged into ribonucleoprotein complex in oocytes and inherited into PGC-fated cells in embryos.However,the maternal factors regulating the assembly of germ plasm and PGC development remain poorly understood.In this study,we report that the maternal transcription factor Znf706 regulates the assembly of germ plasm factors into a granule-like structure localized perinuclearly in PGC during migration.Maternal and zygotic mutants of znf706 exhibit deficient germ plasm scattering at the early embryonic stage,decreased PGC numbers with some mislocation during PGC migration,and a lower female ratio in adulthood.Notably,the implementation of Znf706 CUT&Tag and RNA-seq on immature oocytes uncovers that Znf706 in stage I oocytes may promote transcription of several mitochondrial genes in addition to other functions.Hence,we propose that Znf706 is implicated in germ plasm assembly and PGC development in zebrafish.
基金supported by ITER Project （Nos. 2010GB106000,2010GB106009） National Natural Science Foundation of China （Nos. 10975106, 11175126, 11075114, 11204266）+1 种基金 Qing Lan Project, the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions The Program for Graduates Research ＆： Innovation in University of Jiangsu Province, China （No. CXZZ 11-0085）
文摘The dual-frequency capacitively coupled plasma （DF-CCP） with inductive enhancement system is a newly designed plasma reactor. Different from the conventional inductively coupled plasma （ICP） reactors, now a radio frequency （rf） power is connected to an antenna placed outside the chamber with a one-turn bare coil placed between two electrodes in DF-CCP. This paper gives a detailed description of its structure of discharges in this apparatus were made via a Moreover, investigations on some characteristics Langmuir probe.
基金This work was supported by the National Key Research and Development Program of China and the National Natural Science Foundation of China(NSFC)under Grants No.2020YFA0715000 and No.61825402.
文摘We report a feasible method to realize tun able surface plasm on-polarit on(SPP)res onance in orga nic light-emitt ing devices(OLEDs)by emplo ying corrugated Ag-Al alloy electrodes.The excited SPP res onance in duced by the periodic corrugations can be precisely tuned based on the composition ratios of the Ag-Al alloy electrodes.With an appropriate composition ratio of the corrugated alloy electrode,the photons trapped in SPP modes are recovered and extracted effectively.The 25%in creaseme nt in luminance and 21%enhan ceme nt in curre nt efficie ncy have bee n achieved by using the corrugated Ag-Al alloy electrodes in OLEDs.
文摘The stability of stratified of incompressible, viscoelastic plasma through a porous medium in the presence of the quantum mechanism is considered. The dispersion relation is obtained using the normal mode technique. The behavior of growth rate with respect to the quantum effect, strain retardation time and stress relaxation time are examined in the presence of porosity of the porous medium, the medium permeability, kinematic viscosity. It is shown that, the presence of quantum term stabilizes a certain wave number band, whereas the system is unstable for all wave numbers in the absence of quantum term. The considered parameters beside the quantum term will bring about more stability on the considered system.
文摘BACKGROUND： Inflammatory reaction and the increased level of its accompanying active protein play an important role in the occurrence and development of cerebral infarction. C-reactive protein, fibrinogen and white blood cell, as the monitoring index of inflammatory reaction, are very important in the occurrence and development of acute cerebral infarction. OBJECTIVE： To make a comparison between patients with primary hypertension accompanied with acute cerebral infarction and with simple primary hypertension by observing the changes in plasma C-reactive protein and fibrinogen levels as well as white blood cell and differential counts and analyzing their significances. DESIGN ： Controlled observation SETTING ： Ward Building for VIP, Shenzhen Hospital, Peking University. PARTICIPANTS： Totally 133 patients with primary hypertension were selected from Ward Building for VIP, Shenzhen Hospital, Peking University during September 2003 to September 2005, The diagnostic criteria were based on the hypertension diagnosis criteria formulated by the 7^th World Health Organization-International Society of Hypertension Guidelines （WHO-ISH） in 1998. The informed consents were obtained from all the participants. The involved patients were assigned into two groups： primary hypertension group, in which, there were 65 patients with primary hypertension （ degree 2）, including 42 males and 23 females, with mean age of （61 ±14）years and mean blood pressure of （162.7±6.8）/（94.2±8.4） mm Hg （1 mm Hg =0.133 kPa）, and primary hypertension combined with cerebral infarction group, in which, there were 68 patients with primary hypertension combined with cerebral infarction （ meeting the diagnostic criteria formulated in the 4^th National Cerebrovascular Diseases Meeting in 1995 and diagnosed by skull CT or MRI to exclude the patients with lacunar infarction）, including 42 males and 26 females, with mean age of （56±15） years and mean blood pressure of （176.4±9.2）/（96.3±9.7） mm Hg. METHODS： Plasm C-reactive protein and fibrinogen levels, and white blood cell and differential counts of patients in the two groups were examined 24 hours after stroke. The above indexes were re-examined in the primary hypertension combined with cerebral infarction group 72 hours after stroke. White blood cell and differential counts were performed with laser method （East Asia FE-95001 RAM-1, Japan）. The level of C-reactive protein was measured with turbidimetry （BNII Automatic Systems For Analysis, USA）. The level of fibrinogen was measured with algorithm method when prothrombin time was normal and with Clauss method when prothrombin time was abnormal （ACL Automatic Coagulation Analyzer, USA）. MAIN OUTCOME MEASURES： The plasm C-reactive protein and flbrinogen levels, and white blood cell and differential counts 24 hours after stroke in two groups and 72 hours after stroke in primary hypertension combined with cerebral infarction group. RESULTS： All the 133 involved patients participated in the result analysis. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neutrophil counts in patients with primary hypertension were all within the normal range. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neu- trophil counts in patients with primary hypertension combined with cerebral infarction were significantly higher than those in patients with primary hypertension 24 hours after stroke and 72 hours after stroke respectively[24 hours after stroke：（32.12±11.76） mg/L vs. （5.02±3.21 ） mg/L;（4.64±0.75） g/L vs. （3.12±0.49） g/L; （9.32±81）×10^9 L^- 1 vs. （5.78±1.32）×10^9L^- 1 （7.85±2.38）×10^9 L^- 1 vs.（3.49±1.28）×10^9 L^-1,t =7.094, 5.759,4.106,5.491, respectively,all P〈 0.01; 72 hours after stroke： （47.62±18.43） mg/L vs. （32.12±11.76） mg/L; （5.08±0.82） g/L vs. （4.64±0.75） g/L, t =2.864,2.220, respectively, both P 〈 0.05]. CONCLUSION： The increase in fibrinogen level and white blood cell count are the important index in monitoring primary hypertension combined with acute cerebral infarction. The increase in plasm C-reactive protein and fibrinogen levels 72 hours after stroke indicates that plasma C-reactive protein and fibrinogen are very important in the development of disease.
基金National Natural Science Foundation of China(62131018,12104100)Natural Science Foundation of Guangdong Province(2022A1515010027)+1 种基金Guangzhou Municipal Science and Technology Project(SL2022A04J01205)Nanovision Technology(Beijing)Co.,Ltd.(607230264)。
文摘2D-material-based photodetectors enhanced by plasmonic nanostructures can support responsivity/detectivity several orders higher than commercial photodetectors,drawing extensive attention as promising candidates for the next-generation photodetectors.However,to boost the nanostructure-enhanced 2D photodetectors into real-world applications,crucial challenges lie in the design of broadband enhancing nanostructures and their scalable and position-controllable fabrication.Here,based on a broadband resonant plasmonic disk array fabricated by a scalable and position-controllable technique(direct writing photolithography),we present a visible-near infrared(405-1310 nm)2D WS_(2) photodetector,whose detectivity is up to 3.9×10^(14)Jones,a value exceeding that of the previous plasmon-enhanced 2D photodetectors.The broadened spectral response range and the high detectivity originate from the hot electron injection,optical absorption enhancement,and strain effect supported by the plasmonic array.Furthermore,the designed plasmonic 2D photodetector supports self-powered photodetection,indicating promising potential in energy-free and portable optoelectronic systems.Our results demonstrate an effective method to construct high-performance broadband photodetectors,which can facilitate the development of 2D photodetectors in commercial applications.
文摘When quantum emitters and plasmonic nanoparticles are in close vicinity,the energy exchange,termed as plasmon-exciton coupling,can make the absorption and emission behavior of the hybrid structure very different from those of the two constituents alone.The coupling strength between the two constituents highly depends on how the hybrid structure is constructed.As a result,a diverse range of coupling effect arise including plasmon induced fluorescence quenching/enhancing (weak coupling),Fano interference (intermediate coupling),Rabi-splitting and lasing (strong coupling).The emergence of different coupling behavior can be controlled by the different combinations of quantum emitters and plasmonic nanoparticles as well as the spatial arrangement of the individual components.Colloidal assembly/synthesis methods are essentially delicate strategies that can build the hybrid nanostructures with nanometer precision and allow for large-scale processing.In this review,we discuss the theoretical models that apply to different coupling behaviors,the optical properties of the hybrid systems,and the advancement of colloidal methods to manipulate the plasmon-exciton in the hybrid structures.We also provide perspectives on the challenges and future directions of the research in coupled plasmon-exciton nanosystems.
基金supported by the National Key R&D Program of China(2018YFD0901201 and 2019YFA0802801)China Agricultural Research System(CARS-46)+3 种基金the National Natural Science Foundation of China(31870820)the Innovative Research Group Program of Hubei Province(2020CFA017)the Medical Science Advancement Program of Wuhan University(TFJC2018004)the Fundamental Research Funds for the Central Universities(2042019kf0207)。
文摘Bucky ball(Buc)is involved in germ plasm(GP)assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m^(6)A reader Igf2bp3 interacts and colocalizes with Buc in the GP.Similar to the loss of Buc,the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification,which can be partially restored by the injection of igf2 bp3 mRNA.Igf2bp3 binds to m^(6)A-modified GPorganizer and GP mRNAs in an m^(6)A-dependent manner and prevents their degradation.These findings indicate that the functions of Igf2bp3,a direct effector protein of Buc,in GP mRNA expression and GP assembly involve m^(6)A-dependent regulation;these results emphasize a critical role of m^(6)A modification in the process of GP assembly.
文摘Gangliocytic paraganglioma (GP) is rare and has been regarded as benign in general with a good prognosis. We present a patient with duodenal GP showing a malignant and lethal clinical course. A 47-year-old male patient was found to have a duodenal tumor and enlarged regional lymph nodes. The patient initially underwent a pancreaticoduodenectomy to resect the tumor and involved lymph nodes completely. Histological and immunohistochemical analyses showed findings typical of GP. However, the distant metastatic lesions in the liver and pelvic cavity were rapidly observed after surgery. The patient underwent chemotherapy and radiotherapy, as well as a second surgery to partly remove the metastatic mass in the pelvic cavity. The histological examination revealed no significant difference in histological features between the primary duodenal tumor and the metastatic pelvic mass. However, the patient finally died of the tumor due to the recurrence of the residual pelvic lesion and increased liver mass. To our knowledge, this is the first report of lethal GP with multifocal metastases. Our case confirms that GP should be regarded as a malignant potential tumor with behavior code of “1”, rather than a benign tumor of “0”.
