Genome editing mediated by Clustered regularly interspaced short palindromic repeats(CRISPR)/Cas systems is widely used to study functional genomics and improve the agronomic traits in crops(Hua et al.2019;Gao 2021).T...Genome editing mediated by Clustered regularly interspaced short palindromic repeats(CRISPR)/Cas systems is widely used to study functional genomics and improve the agronomic traits in crops(Hua et al.2019;Gao 2021).To date,various plant-specific CRISPR/Cas expression systems have been developed and applied in model plants,including Arabidopsis,rice,and tobacco,however,editing efficiencies vary widely(Ma et al.2016;Tian et al.2020).展开更多
基金supported by grants from The Agricultural Science and Technology Innovation Program(ASTIP-TRIC02,ASTIP-TRIC-ZD03)Central Public-interest Scientific Institution Basal Research Fund(1610232019001,1610232023006)+1 种基金China Tobacco Genome Project[110202201009(JY-06/-09),110202101036(JY-13)]Key Laboratory of Tobacco Pest Monitoring and Integrated Management(KLTPMIMT2022-03).
文摘Genome editing mediated by Clustered regularly interspaced short palindromic repeats(CRISPR)/Cas systems is widely used to study functional genomics and improve the agronomic traits in crops(Hua et al.2019;Gao 2021).To date,various plant-specific CRISPR/Cas expression systems have been developed and applied in model plants,including Arabidopsis,rice,and tobacco,however,editing efficiencies vary widely(Ma et al.2016;Tian et al.2020).
