The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Rasǒn area of North Korea.The survey was carried out by light microscopic examination of Giemsa-stained blood sme...The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Rasǒn area of North Korea.The survey was carried out by light microscopic examination of Giemsa-stained blood smears,PCR,and phylogenetic evolution analysis of 128 blood samples collected from the Rasǒn area.The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average,respectively,in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears.The detection rate of T.sergenti Giemsa-stained smears was 43.75%,while that with PCR was 67.97%.The detection rate of B.ovata Giemsa-stained smears was 49.21%,while that with PCR was 71.88%.The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T.sergenti isolates from North Korean and that of Yanbian state from China,indicating the closest genetic relationship between both of them.Moreover,100% homology was shown between the 18S rRNA gene sequence of B.ovata isolates from North Korea and the published sequence AY081192 of GenBank,indicating the closest genetic relationship between both of them.This survey confirmed that Ras n is the endemic area of T.sergenti and B.ovata in North Korea.展开更多
羊的梨形虫病在世界范围内广泛分布,由多种泰勒虫和巴贝斯虫引起。在我国主要有莫氏巴贝斯虫、羊巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫,给我国畜牧业造成了巨大的经济损失。为了快速准确地鉴定羊梨形虫,作者运用PCR技术...羊的梨形虫病在世界范围内广泛分布,由多种泰勒虫和巴贝斯虫引起。在我国主要有莫氏巴贝斯虫、羊巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫,给我国畜牧业造成了巨大的经济损失。为了快速准确地鉴定羊梨形虫,作者运用PCR技术扩增了梨形虫的18 S rRNA基因,然后再分别用内切酶ClaⅠ、Sau96Ⅰ和MboⅡ酶切扩增产物,根据酶切后限制性片段多态性的不同,建立了PCR-RFLP方法,达到了鉴定莫氏巴贝斯虫、巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫的目的,为梨形虫的分类和流行病学调查提供了新的方法。展开更多
基金supported by the National Natural Science Foundation of China (30560112, 30960278)Natural Science Foundation of Jilin Province of China (201115230)
文摘The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Rasǒn area of North Korea.The survey was carried out by light microscopic examination of Giemsa-stained blood smears,PCR,and phylogenetic evolution analysis of 128 blood samples collected from the Rasǒn area.The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average,respectively,in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears.The detection rate of T.sergenti Giemsa-stained smears was 43.75%,while that with PCR was 67.97%.The detection rate of B.ovata Giemsa-stained smears was 49.21%,while that with PCR was 71.88%.The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T.sergenti isolates from North Korean and that of Yanbian state from China,indicating the closest genetic relationship between both of them.Moreover,100% homology was shown between the 18S rRNA gene sequence of B.ovata isolates from North Korea and the published sequence AY081192 of GenBank,indicating the closest genetic relationship between both of them.This survey confirmed that Ras n is the endemic area of T.sergenti and B.ovata in North Korea.
文摘羊的梨形虫病在世界范围内广泛分布,由多种泰勒虫和巴贝斯虫引起。在我国主要有莫氏巴贝斯虫、羊巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫,给我国畜牧业造成了巨大的经济损失。为了快速准确地鉴定羊梨形虫,作者运用PCR技术扩增了梨形虫的18 S rRNA基因,然后再分别用内切酶ClaⅠ、Sau96Ⅰ和MboⅡ酶切扩增产物,根据酶切后限制性片段多态性的不同,建立了PCR-RFLP方法,达到了鉴定莫氏巴贝斯虫、巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫的目的,为梨形虫的分类和流行病学调查提供了新的方法。
