Objectives:To explore the effect of Pollen Pini(PP) on SMMC-7721 hepatoma cells.Methods:The anti-proliferative effects of PP on SMMC-7721 cells were evaluated using cell counting kit-8 assay following 48 h treatment w...Objectives:To explore the effect of Pollen Pini(PP) on SMMC-7721 hepatoma cells.Methods:The anti-proliferative effects of PP on SMMC-7721 cells were evaluated using cell counting kit-8 assay following 48 h treatment with concentrations ranging from 1.25 to 37.50 μg/μL.Flow cytometry analysis at the half maximal inhibitory concentration(IC_(50)) revealed significant apoptosis induction and cell cycle alterations.For in vivo evaluation,SMMC-7721 xenograft-bearing nude mice were administered either vehicle(0.9% NaCl) or PP(500 mg/kg) via intraperitoneal injection every other day for 3 weeks.Subsequent tumor analysis included mass spectrometry-based proteomics and examination of phosphoinositide-3 kinase(PI3K)/protein kinase B(Akt)/Myc pathway proteins by immunohistochemistry and Western blot.Combination therapy with 25 μmol/L PI3K inhibitor and PP(IC_(50)) showed optimal efficacy,with Western blot revealing maximal protein modulation at 24 h.Results:PP had an anti-tumor effect on SMMC-7721 cells in vitro,with the IC_(50) concentration of 18.94 mg/mL.PP could promote the death of SMMC-7721 cells(P<0.01) and regulate the cell cycle more in G_(0)/G_(1) phase cells(P<0.01).After the treatment effect of PP,the protein content of SMMC-7721 cells changed and the contents of cancer-promoting proteins PI3K,Akt and Myc decreased(P<0.01).RI3K inhibitor could reduce the proliferation of SMMC-7721cells,and PI3K inhibitor combined with PP significantly reduced the expression of PI3K in SMMC-7721 cells(P<0.01).Conclusion:PP has an anti-tumor effect on SMMC-7721 cells through the PI3K-Akt signaling pathway.展开更多
The indoor toxicity determination for several chemicals were carried out on Septoria pini-putmlae andDothistroma pini. The resutts showed that Carbendazol, Zineb and Thiophanate-methyl had better effect of killingthe ...The indoor toxicity determination for several chemicals were carried out on Septoria pini-putmlae andDothistroma pini. The resutts showed that Carbendazol, Zineb and Thiophanate-methyl had better effect of killingthe fungi. The field experiment indicated that Zineb wettable powder with concentrations of 1:200, 1:300 and 1:500had 79.2%, 67.2% and 59.7% control results respectively. The control results of 1:200 Carbendazol wettable powder and 1:200 Thiophahate-methyl wettable powder were 67.7% and 67% respectively.展开更多
Fixed quadrates were established in different stands. In continued six years, the occurring period, occurring amounts and the relation between epidemic disease and environmental factors were investigated according to ...Fixed quadrates were established in different stands. In continued six years, the occurring period, occurring amounts and the relation between epidemic disease and environmental factors were investigated according to spraying lawsof spores and accounting measures of disease ranking. The occuring peak period of the disease was from the last ten days ofMay to the second ten days of June. The epidemic period was from the last ten days of June to the second ten days of Julyand the initial decease period was from the last ten days of July to the beginning of September. The change of the diseasedepended on air temperature, relative humidity and precipitation. A multiple linear regression model was established usingcomputer, which can predict the disease index(Y) of 10 days later, with more than 95% reliability展开更多
基金Supported by the National Natural Science Foundation of China (No. 81960303)。
文摘Objectives:To explore the effect of Pollen Pini(PP) on SMMC-7721 hepatoma cells.Methods:The anti-proliferative effects of PP on SMMC-7721 cells were evaluated using cell counting kit-8 assay following 48 h treatment with concentrations ranging from 1.25 to 37.50 μg/μL.Flow cytometry analysis at the half maximal inhibitory concentration(IC_(50)) revealed significant apoptosis induction and cell cycle alterations.For in vivo evaluation,SMMC-7721 xenograft-bearing nude mice were administered either vehicle(0.9% NaCl) or PP(500 mg/kg) via intraperitoneal injection every other day for 3 weeks.Subsequent tumor analysis included mass spectrometry-based proteomics and examination of phosphoinositide-3 kinase(PI3K)/protein kinase B(Akt)/Myc pathway proteins by immunohistochemistry and Western blot.Combination therapy with 25 μmol/L PI3K inhibitor and PP(IC_(50)) showed optimal efficacy,with Western blot revealing maximal protein modulation at 24 h.Results:PP had an anti-tumor effect on SMMC-7721 cells in vitro,with the IC_(50) concentration of 18.94 mg/mL.PP could promote the death of SMMC-7721 cells(P<0.01) and regulate the cell cycle more in G_(0)/G_(1) phase cells(P<0.01).After the treatment effect of PP,the protein content of SMMC-7721 cells changed and the contents of cancer-promoting proteins PI3K,Akt and Myc decreased(P<0.01).RI3K inhibitor could reduce the proliferation of SMMC-7721cells,and PI3K inhibitor combined with PP significantly reduced the expression of PI3K in SMMC-7721 cells(P<0.01).Conclusion:PP has an anti-tumor effect on SMMC-7721 cells through the PI3K-Akt signaling pathway.
文摘The indoor toxicity determination for several chemicals were carried out on Septoria pini-putmlae andDothistroma pini. The resutts showed that Carbendazol, Zineb and Thiophanate-methyl had better effect of killingthe fungi. The field experiment indicated that Zineb wettable powder with concentrations of 1:200, 1:300 and 1:500had 79.2%, 67.2% and 59.7% control results respectively. The control results of 1:200 Carbendazol wettable powder and 1:200 Thiophahate-methyl wettable powder were 67.7% and 67% respectively.
文摘Fixed quadrates were established in different stands. In continued six years, the occurring period, occurring amounts and the relation between epidemic disease and environmental factors were investigated according to spraying lawsof spores and accounting measures of disease ranking. The occuring peak period of the disease was from the last ten days ofMay to the second ten days of June. The epidemic period was from the last ten days of June to the second ten days of Julyand the initial decease period was from the last ten days of July to the beginning of September. The change of the diseasedepended on air temperature, relative humidity and precipitation. A multiple linear regression model was established usingcomputer, which can predict the disease index(Y) of 10 days later, with more than 95% reliability
