This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for...This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for treating real municipal wastewater.The results showed that the changing rate( S)of the peak area(S)of granule size distribution(GSD)exhibited an exponential relationship(R^(2)≥0.76)with the shear time(y=a−b·c^(x)),which was a suitable indicative index to reflect the shear stability of different AGS samples.The limiting granule size(LGS)was defined and proposed to characterize the equilibrium size for AGS after being sheared for a period of time,whose value in terms of Dv50 showed high correlation(R^(2)=0.92)with the parameter a.The free Ca^(2+)(28.44-34.21 mg/L)in the influent specifically interacted with polysaccharides(PS)in the granule’s extracellular polymeric substance(EPS)as a nucleation site,thereby inducing the formation of Ca precipitation to enhance its Young’s modulus,while Ca^(2+) primarily interacted with PS in soluble metabolic product(SMP)during the initial granulation process.Furthermore,the Young’s modulus significantly affected the parameter a related to shear stability(R^(2)=0.99).Since the parameter a was more closely related(R^(2)=1.00)to S than that of the parameter b or c,the excellent correlation(R^(2)=0.99)between the parameter a and the wet density further verified the feasibility of this method.展开更多
The widespread use of pesticides has caused serious harm to ecosystems,necessitating effective and environmentally friendly treatment methods.Bioremediation stands out as a promising approach for pollutant treatment,w...The widespread use of pesticides has caused serious harm to ecosystems,necessitating effective and environmentally friendly treatment methods.Bioremediation stands out as a promising approach for pollutant treatment,wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity.In this study,we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening.Four highly efficient pesticide-degrading strains(degradation ratios exceeding 80%)were identified.Among them,Pseudomonas sp.BL5 exhibited the strongest growth(exceeding 10^(9) CFU·ml^(-1))and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales,with degradation ratios exceeding 98%and 99.6%,respectively.This research provides new tools and insights for the bioremediation of pesticide-related pollutants.展开更多
To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and...To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and decrease the energy cost further,we report a new carbon capture approach using a 2-methylimidazole(mIm)aqueous solution.The properties and sorption behaviors of this approach have been experimentally investigated.The results show that the mIm solution has higher CO_(2) absorption capacity under relatively higher equilibrium pressure(>130 kPa)and lower desorption heat than the methyldiethanolamine solution.91.6%sorption capacity of mIm solution can be recovered at 353.15 K and 80 kPa.The selectivity for CO_(2)/N_(2) and CO_(2)/CH_(4) can reach an exceptional 7609 and 4324,respectively.Furthermore,the pilot-scale tests were also performed,and the results demonstrate that more than 98%of CO_(2) in the feed gas could be removed and cyclic absorption capacity can reach 1 mol·L^(-1).This work indicates that mIm is an excellent alternative to alkanolamines for carbon capture in the industry.展开更多
In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-st...In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-stage anoxic-oxic (A/O) process based on previous lab-scale study. Experimental results showed that when the concentrations of COD and NH4+-N of the influent were 370~910 mg/L and 10~70 mg/L, the corresponding average concentrations of those of effluent were about 80 mg/L and 8 mg/L respectively, which was better than the Level I criteria of the Integrated Wastewater Discharge Standard (GB8978-1996). According to GC-MS analysis of the effluents from both the wastewater treatment plant (WWTP) and the pilot system, there were 68 kinds of persistent organic pollutants in the WWTP effluent, while there were only 32 in that of the pilot system. In addition, the amount of the organics in the effluent of the pilot system reduced by almost 50% compared to that of the WWTP. As a whole, after bioaugmentation, the organic removal efficiency of the wastewater treatment system obviously in- creased.展开更多
Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were ...Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.展开更多
Tertiary denitrification is an effective method for nitrogen removal from wastewater. A pilot-scale biofilter packed with suspended carriers was operated for tertiary denitrification with ethanol as the organic carbon...Tertiary denitrification is an effective method for nitrogen removal from wastewater. A pilot-scale biofilter packed with suspended carriers was operated for tertiary denitrification with ethanol as the organic carbon source. Long-term performance, biokinetics of denitrification and biofilm growth were evaluated under filtration velocities of 6, 10 and 14 m/hr. The pilot-scale biofilter removed nitrate from the secondary effluent effectively, and the nitrate nitrogen(NO_3-N) removal percentage was 82%, 78% and 55% at the filtration velocities of 6, 10 and 14 m/hr, respectively. At the filtration velocities of 6 and 10 m/hr, the nitrate removal loading rate increased with increasing influent nitrate loading rates, while at the filtration velocity of 14 m/hr, the removal loading rate and the influent loading rate were uncorrelated.During denitrification, the ratio of consumed chemical oxygen demand to removed NO_3-N was 3.99-4.52 mg/mg. Under the filtration velocities of 6, 10 and 14 m/hr, the maximum denitrification rate was 3.12, 4.86 and 4.42 g N/(m^2·day), the half-saturation constant was 2.61, 1.05 and 1.17 mg/L, and the half-order coefficient was 0.22, 0.32 and 0.24(mg/L)1/2/min,respectively. The biofilm biomass increased with increasing filtration velocity and was 2845,5124 and 7324 mg VSS/m^2 at filtration velocities of 6, 10 and 14 m/hr, respectively. The highest biofilm density was 44 mg/cm^3 at the filtration velocity of 14 m/hr. Due to the low influent loading rate, biofilm biomass and thickness were lowest at the filtration velocity of 6 m/hr.展开更多
[ Objective] This study aimed to explore the effect of C/N ratio on DHA production by Schizochytrium sp. , and the results were verified by pilot-scale fermentation. [ Method] Effect of C/N ratio on DHA production by ...[ Objective] This study aimed to explore the effect of C/N ratio on DHA production by Schizochytrium sp. , and the results were verified by pilot-scale fermentation. [ Method] Effect of C/N ratio on DHA production by Schizochytrium sp. JN-3 was explored. The industrial fermentation potential of Schizochytrium sp. JN-3 was investigated by pilot-scale fermentation in a 200 L fermentation tank. [ Result] Results of fermentation in a 7 L fermentation tank showed that the maximum biomass and total lipid content were 34.3 and 20.1 g/L, respectively, when C/N ratio was 10 ; the maximum DHA yield was 46.9% when C/N ratio was 20. When pilot-scale fermentation was conducted in a 200 L fermentation tank, the biomass was 30. 2 g/L, total lipid content was 18.4 g/L, accounting for 63.2% of the dry weight of cells; DHA content was 7.4 g/L, accounting for 40. 2% of the total lipid content. [ Condasion ] Schizochytrlum sp. JN-3 has good industrial fermentation notential.展开更多
Industrial wastewater from modern industrial production often contains excessive organic hazardous substances or excessive salts, acids and bases, etc. Traditional methods cannot play an effective role in the treatmen...Industrial wastewater from modern industrial production often contains excessive organic hazardous substances or excessive salts, acids and bases, etc. Traditional methods cannot play an effective role in the treatment of such wastewater. Moreover, since such wastewater is also not suitable for the growth of microorganisms, the way of wastewater treatment by microorganisms is also greatly limited. For this kind of industrial waste water, apart from the degradation of organic matters, the separation of inorganic salts and waste water is also required to meet the sewage discharge standard. Based on this, the article focuses on the coal chemical high salt wastewater electro-catalytic oxidation pilot study.展开更多
In the microalgae harvesting process,which includes a step for dewatering the algal suspension,directly reusing extracted water in situ would decrease the freshwater footprint of cultivation systems.Among various alga...In the microalgae harvesting process,which includes a step for dewatering the algal suspension,directly reusing extracted water in situ would decrease the freshwater footprint of cultivation systems.Among various algae harvesting techniques,membrane-based filtration has shown numerous advantages.This study evaluated the reuse of permeate streams derived from Scenedesmus obliquus(S.obliquus)biomass filtration under bench-scale and pilot-scale conditions.In particular,this study identified a series of challenges and mechanisms that influence the water reuse potential and the robustness of the membrane harvesting system.In a preliminary phase of this investigation,the health status of the initial biomass was found to have important implications for the harvesting performance and quality of the permeate stream to be reused;healthy biomass ensured better dewatering performance(i.e.,higher water fluxes)and higher quality of the permeate water streams.A series of bench-scale filtration experiments with different combinations of cross-flow velocity and pressure values were performed to identify the operative conditions that would maximize water productivity.The selected conditions,2.4 m·s^(-1)and 1.4 bar(1 bar=105 Pa),respectively,were then applied to drive pilot-scale microfiltration tests to reuse the collected permeate as a new cultivation medium for S.obliquus growth in a pilot-scale photobioreactor.The investigation revealed key differences between the behavior of the membrane systems at the two scales(bench and pilot).It indicated the potential for beneficial reuse of the permeate stream as the pilot-scale experiments ensured high harvesting performance and growth rates of biomass in permeate water that were highly similar to those recorded in the ideal cultivation medium.Finally,different nutrient reintegration protocols were investigated,revealing that both macro-and micro-nutrient levels are critical for the success of the reuse approach.展开更多
The experiment system of 1000 m deep sea mining system is built up with the similarity theory. Sine mechanism is used to simulate mining ship to generate lateral shock excitation by ocean wave. Simulation and experime...The experiment system of 1000 m deep sea mining system is built up with the similarity theory. Sine mechanism is used to simulate mining ship to generate lateral shock excitation by ocean wave. Simulation and experiment of spherical joint connecting deep sea mining system has been done in band six marine conditions. The results indicate that the moment of spherical joint connecting deep sea mining is smaller than that of the thread connected ones, the lifting pipe of sphelical joint is "flexible pipe". The flexural torque of the articulated lifting pipe system in pump and buffer is generally periodic variation with some irregularity, the value is stable on 60 N·S, and it is obviously smaller than that of the fixed lifting pipe system; The total displacement exhibits cyclic variation pattern, and the periodicity of them is longer than that of sea current. The results of experiment and simulation are basically consistent. And the analysis in the paper offers theoretical foundation of 1000 m deep sea mining system in China.展开更多
基金supported by the Key Technologies Research and Development Program of Guangzhou (No.2023B03J1284)Guangdong Yuehai Water Investment Co.,Ltd. (No.CC80-QT01-2020-0010).
文摘This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for treating real municipal wastewater.The results showed that the changing rate( S)of the peak area(S)of granule size distribution(GSD)exhibited an exponential relationship(R^(2)≥0.76)with the shear time(y=a−b·c^(x)),which was a suitable indicative index to reflect the shear stability of different AGS samples.The limiting granule size(LGS)was defined and proposed to characterize the equilibrium size for AGS after being sheared for a period of time,whose value in terms of Dv50 showed high correlation(R^(2)=0.92)with the parameter a.The free Ca^(2+)(28.44-34.21 mg/L)in the influent specifically interacted with polysaccharides(PS)in the granule’s extracellular polymeric substance(EPS)as a nucleation site,thereby inducing the formation of Ca precipitation to enhance its Young’s modulus,while Ca^(2+) primarily interacted with PS in soluble metabolic product(SMP)during the initial granulation process.Furthermore,the Young’s modulus significantly affected the parameter a related to shear stability(R^(2)=0.99).Since the parameter a was more closely related(R^(2)=1.00)to S than that of the parameter b or c,the excellent correlation(R^(2)=0.99)between the parameter a and the wet density further verified the feasibility of this method.
基金supported by the National Key Research and Development Program of China(2018YFA0902100)the National Natural Science Foundation of China(22178262)the Tianjin Key Research and Development Program(23YFZCSN00110).
文摘The widespread use of pesticides has caused serious harm to ecosystems,necessitating effective and environmentally friendly treatment methods.Bioremediation stands out as a promising approach for pollutant treatment,wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity.In this study,we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening.Four highly efficient pesticide-degrading strains(degradation ratios exceeding 80%)were identified.Among them,Pseudomonas sp.BL5 exhibited the strongest growth(exceeding 10^(9) CFU·ml^(-1))and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales,with degradation ratios exceeding 98%and 99.6%,respectively.This research provides new tools and insights for the bioremediation of pesticide-related pollutants.
基金The financial supports received from National Natural Science Foundation of China (U20B6005, 22178378, and 22127812)
文摘To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and decrease the energy cost further,we report a new carbon capture approach using a 2-methylimidazole(mIm)aqueous solution.The properties and sorption behaviors of this approach have been experimentally investigated.The results show that the mIm solution has higher CO_(2) absorption capacity under relatively higher equilibrium pressure(>130 kPa)and lower desorption heat than the methyldiethanolamine solution.91.6%sorption capacity of mIm solution can be recovered at 353.15 K and 80 kPa.The selectivity for CO_(2)/N_(2) and CO_(2)/CH_(4) can reach an exceptional 7609 and 4324,respectively.Furthermore,the pilot-scale tests were also performed,and the results demonstrate that more than 98%of CO_(2) in the feed gas could be removed and cyclic absorption capacity can reach 1 mol·L^(-1).This work indicates that mIm is an excellent alternative to alkanolamines for carbon capture in the industry.
基金Project supported by the National Basic Research Program (973) of China (No. 2004CB418505)the Science and Technology Development Program of Heilongjiang Province (No. CC05S301), China
文摘In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-stage anoxic-oxic (A/O) process based on previous lab-scale study. Experimental results showed that when the concentrations of COD and NH4+-N of the influent were 370~910 mg/L and 10~70 mg/L, the corresponding average concentrations of those of effluent were about 80 mg/L and 8 mg/L respectively, which was better than the Level I criteria of the Integrated Wastewater Discharge Standard (GB8978-1996). According to GC-MS analysis of the effluents from both the wastewater treatment plant (WWTP) and the pilot system, there were 68 kinds of persistent organic pollutants in the WWTP effluent, while there were only 32 in that of the pilot system. In addition, the amount of the organics in the effluent of the pilot system reduced by almost 50% compared to that of the WWTP. As a whole, after bioaugmentation, the organic removal efficiency of the wastewater treatment system obviously in- creased.
基金Supported by the National Basic Research Program of China(“973”Program:2014CB745100)the National Natural Science Foundation of China(21576197)+2 种基金Tianjin Research Program of Application Foundation and Advanced Technology(14JCQNJC06700)the Major Research Plan of Tianjin(16YFXTSF00460)Tianjin Penglai 19-3 Oil Spill Accident Compensation Project(19-3BC2014-03)
文摘Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China (No. 2012ZX07302002)
文摘Tertiary denitrification is an effective method for nitrogen removal from wastewater. A pilot-scale biofilter packed with suspended carriers was operated for tertiary denitrification with ethanol as the organic carbon source. Long-term performance, biokinetics of denitrification and biofilm growth were evaluated under filtration velocities of 6, 10 and 14 m/hr. The pilot-scale biofilter removed nitrate from the secondary effluent effectively, and the nitrate nitrogen(NO_3-N) removal percentage was 82%, 78% and 55% at the filtration velocities of 6, 10 and 14 m/hr, respectively. At the filtration velocities of 6 and 10 m/hr, the nitrate removal loading rate increased with increasing influent nitrate loading rates, while at the filtration velocity of 14 m/hr, the removal loading rate and the influent loading rate were uncorrelated.During denitrification, the ratio of consumed chemical oxygen demand to removed NO_3-N was 3.99-4.52 mg/mg. Under the filtration velocities of 6, 10 and 14 m/hr, the maximum denitrification rate was 3.12, 4.86 and 4.42 g N/(m^2·day), the half-saturation constant was 2.61, 1.05 and 1.17 mg/L, and the half-order coefficient was 0.22, 0.32 and 0.24(mg/L)1/2/min,respectively. The biofilm biomass increased with increasing filtration velocity and was 2845,5124 and 7324 mg VSS/m^2 at filtration velocities of 6, 10 and 14 m/hr, respectively. The highest biofilm density was 44 mg/cm^3 at the filtration velocity of 14 m/hr. Due to the low influent loading rate, biofilm biomass and thickness were lowest at the filtration velocity of 6 m/hr.
基金Supported by Research Fund for the Doctoral Program of Ministry of Education(20100093120002)Open Project of the Key Laboratory of Industrial Biotechnology under Ministry of Education(KLIB-KF200906,KLIB-KF201005)
文摘[ Objective] This study aimed to explore the effect of C/N ratio on DHA production by Schizochytrium sp. , and the results were verified by pilot-scale fermentation. [ Method] Effect of C/N ratio on DHA production by Schizochytrium sp. JN-3 was explored. The industrial fermentation potential of Schizochytrium sp. JN-3 was investigated by pilot-scale fermentation in a 200 L fermentation tank. [ Result] Results of fermentation in a 7 L fermentation tank showed that the maximum biomass and total lipid content were 34.3 and 20.1 g/L, respectively, when C/N ratio was 10 ; the maximum DHA yield was 46.9% when C/N ratio was 20. When pilot-scale fermentation was conducted in a 200 L fermentation tank, the biomass was 30. 2 g/L, total lipid content was 18.4 g/L, accounting for 63.2% of the dry weight of cells; DHA content was 7.4 g/L, accounting for 40. 2% of the total lipid content. [ Condasion ] Schizochytrlum sp. JN-3 has good industrial fermentation notential.
文摘Industrial wastewater from modern industrial production often contains excessive organic hazardous substances or excessive salts, acids and bases, etc. Traditional methods cannot play an effective role in the treatment of such wastewater. Moreover, since such wastewater is also not suitable for the growth of microorganisms, the way of wastewater treatment by microorganisms is also greatly limited. For this kind of industrial waste water, apart from the degradation of organic matters, the separation of inorganic salts and waste water is also required to meet the sewage discharge standard. Based on this, the article focuses on the coal chemical high salt wastewater electro-catalytic oxidation pilot study.
基金supported by the Politecnico di Torino and the CleanWaterCenter@PoliTo(58_DIM20TIRALB,58_DIM22TIRALB,and 01_TRIN_CI_CWC).
文摘In the microalgae harvesting process,which includes a step for dewatering the algal suspension,directly reusing extracted water in situ would decrease the freshwater footprint of cultivation systems.Among various algae harvesting techniques,membrane-based filtration has shown numerous advantages.This study evaluated the reuse of permeate streams derived from Scenedesmus obliquus(S.obliquus)biomass filtration under bench-scale and pilot-scale conditions.In particular,this study identified a series of challenges and mechanisms that influence the water reuse potential and the robustness of the membrane harvesting system.In a preliminary phase of this investigation,the health status of the initial biomass was found to have important implications for the harvesting performance and quality of the permeate stream to be reused;healthy biomass ensured better dewatering performance(i.e.,higher water fluxes)and higher quality of the permeate water streams.A series of bench-scale filtration experiments with different combinations of cross-flow velocity and pressure values were performed to identify the operative conditions that would maximize water productivity.The selected conditions,2.4 m·s^(-1)and 1.4 bar(1 bar=105 Pa),respectively,were then applied to drive pilot-scale microfiltration tests to reuse the collected permeate as a new cultivation medium for S.obliquus growth in a pilot-scale photobioreactor.The investigation revealed key differences between the behavior of the membrane systems at the two scales(bench and pilot).It indicated the potential for beneficial reuse of the permeate stream as the pilot-scale experiments ensured high harvesting performance and growth rates of biomass in permeate water that were highly similar to those recorded in the ideal cultivation medium.Finally,different nutrient reintegration protocols were investigated,revealing that both macro-and micro-nutrient levels are critical for the success of the reuse approach.
基金This research was financially supported by China Ocean Mineral Resources Reasearch and Design Association (GrantNo DY105-03-02-17)Ph.D Programs Foundation of Ministry of Education of China (Grant No20060008025)
文摘The experiment system of 1000 m deep sea mining system is built up with the similarity theory. Sine mechanism is used to simulate mining ship to generate lateral shock excitation by ocean wave. Simulation and experiment of spherical joint connecting deep sea mining system has been done in band six marine conditions. The results indicate that the moment of spherical joint connecting deep sea mining is smaller than that of the thread connected ones, the lifting pipe of sphelical joint is "flexible pipe". The flexural torque of the articulated lifting pipe system in pump and buffer is generally periodic variation with some irregularity, the value is stable on 60 N·S, and it is obviously smaller than that of the fixed lifting pipe system; The total displacement exhibits cyclic variation pattern, and the periodicity of them is longer than that of sea current. The results of experiment and simulation are basically consistent. And the analysis in the paper offers theoretical foundation of 1000 m deep sea mining system in China.