[ Objective] To prepare recombinant type 1 pilus vaccine of avian Escherichia coil and to detect its immunogenicity in chickens. [Meth- od] The type 1 pilus was respectively isolated from the recombinant bacteria CZYR...[ Objective] To prepare recombinant type 1 pilus vaccine of avian Escherichia coil and to detect its immunogenicity in chickens. [Meth- od] The type 1 pilus was respectively isolated from the recombinant bacteria CZYR10 strain and wild avian Eschedchia co/i YR（ O18 ） strain and used to prepare oil-emulsified vaccine. The immunogenicity of the developed vaccine was detected in chickens using the challenge test. [ Result] The recombinant type 1 pilus protein had immunoprotective efficacy. The recombinant type 1 pilus protein had weaker protective efficacy than the isolated type 1 pilus protein, but the difference was not significant. [ Conclusion] The study provides a reference for immunization and control of chicken colibacillosis.展开更多
In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial behaviors.While PilU isconsidered a homolog of the TFP disassembling motor PilT,its specific roles remain unclear.U...In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial behaviors.While PilU isconsidered a homolog of the TFP disassembling motor PilT,its specific roles remain unclear.Using pilus visualization andsingle-cell tracking techniques,we characterized TFP dynamics and surface behaviors in wild-type andΔpilU mutants.Wefound thatΔpilU cells displayed increased TFP numbers but reduced cell movement and delayed microcolony formation.Interestingly,beyond affecting the twitching motility,ΔpilU cells formed a thick multilayered colony edge on semi-solid sur-faces,slowing colony expansion.Cell-cell collision responses changed from touch-turn dominance in wild type to touch-upright dominance inΔpilU,affecting colony morphology and expansion.Thesefindings expand our understanding of PilU'sphysiological roles and provide potential targets for developing strategies to control P.aeruginosa biofilm formation andvirulence.展开更多
基金funded by the Natural Science Foundation of Jiangsu Province ( BK2008059)
文摘[ Objective] To prepare recombinant type 1 pilus vaccine of avian Escherichia coil and to detect its immunogenicity in chickens. [Meth- od] The type 1 pilus was respectively isolated from the recombinant bacteria CZYR10 strain and wild avian Eschedchia co/i YR（ O18 ） strain and used to prepare oil-emulsified vaccine. The immunogenicity of the developed vaccine was detected in chickens using the challenge test. [ Result] The recombinant type 1 pilus protein had immunoprotective efficacy. The recombinant type 1 pilus protein had weaker protective efficacy than the isolated type 1 pilus protein, but the difference was not significant. [ Conclusion] The study provides a reference for immunization and control of chicken colibacillosis.
基金Supported by the National Natural ScienceFoundation of China(12374206)to K.Z.National Key R&DProgram of China(2023YFC3402401)to K.Z.+2 种基金National KeyR&D Program of China(2020YFA0906900)to F.J.China,Postdoctoral Science Foundation(2023M740523)to J.Z.Sichuan Provincial People's Hospital Research Fund ofthe Academy(2022B1013)to J.Z。
文摘In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial behaviors.While PilU isconsidered a homolog of the TFP disassembling motor PilT,its specific roles remain unclear.Using pilus visualization andsingle-cell tracking techniques,we characterized TFP dynamics and surface behaviors in wild-type andΔpilU mutants.Wefound thatΔpilU cells displayed increased TFP numbers but reduced cell movement and delayed microcolony formation.Interestingly,beyond affecting the twitching motility,ΔpilU cells formed a thick multilayered colony edge on semi-solid sur-faces,slowing colony expansion.Cell-cell collision responses changed from touch-turn dominance in wild type to touch-upright dominance inΔpilU,affecting colony morphology and expansion.Thesefindings expand our understanding of PilU'sphysiological roles and provide potential targets for developing strategies to control P.aeruginosa biofilm formation andvirulence.
