Lacticaseibacillus rhamnosus Lr-CPU2205,isolated from traditional Sichuan pickled vegetables,demonstrates promising probiotic properties,as revealed through integrated genomic and phenotypic analyses.The strain exhibi...Lacticaseibacillus rhamnosus Lr-CPU2205,isolated from traditional Sichuan pickled vegetables,demonstrates promising probiotic properties,as revealed through integrated genomic and phenotypic analyses.The strain exhibited robust gastrointestinal tolerance,with 70.4%survival at pH 4.0 and 31%survival in the presence of 0.1%bile salts.It also showed significant in vitro antibacterial activity against Salmonella Typhimurium and Escherichia coli,along with potent free radical scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)(20.47%),hydroxyl(46.74%),and superoxide anion(38.09%)radicals.Whole-genome sequencing identified a 2.96 Mbp chromosome encoding key genes involved in carbohydrate metabolism(e.g.,Carbohydrate-active enzymes(CAZymes)),stress adaptation(e.g.,nhaK,napA,bsaA),and antimicrobial peptide synthesis.In vivo studies using Caenorhabditis elegans revealed that Lr-CPU2205 enhanced resistance to S.Typhimurium infection,improved oxidative stress survival under H_(2)O_(2)challenge(50%lifespan extension),and extended normal life-span by 42.11%.Safety assessments indicated no detectable virulence factors and general antibiotic suscepti-bility,except for intrinsic resistance to co-trimoxazole and ampicillin.These findings highlight L.rhamnosus Lr-CPU2205 as a safe and multi-functional probiotic candidate suitable for application in functional foods and health-promoting formulations.展开更多
Antimicrobial resistance(AMR)is a public health problem of global concern.The presence of antibiotic resistance genes(ARGs)in food may aggravate the spread of AMR.Understanding the distribution and transfer of drug-re...Antimicrobial resistance(AMR)is a public health problem of global concern.The presence of antibiotic resistance genes(ARGs)in food may aggravate the spread of AMR.Understanding the distribution and transfer of drug-resistant bacteria and ARGs in pickled vegetable foods was critical for food quality and safety.Therefore,this study used metagenomic sequencing,binning technology,and antibiotic susceptibility tests to explore the distribution and transfer of drug-resistant bacteria and ARGs in pickled vegetable foods.The results showed that Bacillota,Lactobacillus,and Salmonella were the dominant bacteria in all samples.A total of 12 ARG types and 52 ARG subtypes were identified.The abundance of bacitracin ARGs in high-salt samples(<2%)was higher,and multidrug ARGs in low-salt samples(7%-10%)was higher.The most abundant ARG subtypes,baca and acrb,were associated with Salmonella.Furthermore,a total of 34 metagenome assembly genomes(MAGs)were obtained by metagenomic binning.MAG8 and MAG25,which carried the largest number of ARGs,virulence factors(VFs),and MGEs,were all derived from Vibrio.More importantly,horizontal gene transfer(HGT)could occur between microorganisms in pickled vegetable samples.In addition,most of the 25 isolates were resistant to bacitracin and multidrug antibiotics.Levilactobacillus brevis PNS3 carried a large number of multidrug ARG genes(patA,macB)and transposable MGE genes(recT,SadA).The results of the drug resistance phenotype were consistent with the results of the drug resistance genotype.These findings suggested that there was a risk of ARGs being carrying and spreading in pickled vegetable foods.展开更多
Background Maternal exposure to nitrate, nitrite, and N-nitroso compounds from drinking water or diet has been associated with an increased risk of neural tube defects （NTDs） in some studies. Pickled vegetables cont...Background Maternal exposure to nitrate, nitrite, and N-nitroso compounds from drinking water or diet has been associated with an increased risk of neural tube defects （NTDs） in some studies. Pickled vegetables contain relatively large amounts of nitrite and N-nitroso compounds. We examined the association between maternal periconceptional consumption of pickled vegetables and NTDs in Shanxi Province of northern China. Methods Data were derived from a population based case-control study of major external birth defects in four counties of $hanxi Province. Participants included 519 NTDs cases identified between 2003 and 2007 and 694 healthy controls. Exposure information was collected within 1 week after delivery. Multivariable non-conditioanal Logistic regression model was used to estimate the adjusted odds ratio （OR） controlling for potential confounding variables.展开更多
Salicylic acid(SA)is used as a preservative in food and a biocide in some consumer products.However,in some countries its use is prohibited.The main problem in the electroanalytical determination of SA is the formatio...Salicylic acid(SA)is used as a preservative in food and a biocide in some consumer products.However,in some countries its use is prohibited.The main problem in the electroanalytical determination of SA is the formation of a passive film during the electro-oxidation of SA.To alleviate the passivation during SA measurement,the experiment was conducted by screen-printed carbon electrode(SPCE).While,differential pulse voltammetry(DPV)was essential for oxidation process to provide better sensitivity.Under conditions of optimized pH and electrolyte concentration,we have calibrated SA in the range of 0.001-0.2 mM,with and LOD of 1.6μM.The relative standard deviation of the sensor responses to 40μM and 100μM SA(n=20)was<3%.SA recoveries in samples such as pickled green mustard,pickled bamboo shoots,pickled mango,pickled lime,pickle ginger and apple juice were found to be in the range of 84-113%.展开更多
基金funded by Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Lacticaseibacillus rhamnosus Lr-CPU2205,isolated from traditional Sichuan pickled vegetables,demonstrates promising probiotic properties,as revealed through integrated genomic and phenotypic analyses.The strain exhibited robust gastrointestinal tolerance,with 70.4%survival at pH 4.0 and 31%survival in the presence of 0.1%bile salts.It also showed significant in vitro antibacterial activity against Salmonella Typhimurium and Escherichia coli,along with potent free radical scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)(20.47%),hydroxyl(46.74%),and superoxide anion(38.09%)radicals.Whole-genome sequencing identified a 2.96 Mbp chromosome encoding key genes involved in carbohydrate metabolism(e.g.,Carbohydrate-active enzymes(CAZymes)),stress adaptation(e.g.,nhaK,napA,bsaA),and antimicrobial peptide synthesis.In vivo studies using Caenorhabditis elegans revealed that Lr-CPU2205 enhanced resistance to S.Typhimurium infection,improved oxidative stress survival under H_(2)O_(2)challenge(50%lifespan extension),and extended normal life-span by 42.11%.Safety assessments indicated no detectable virulence factors and general antibiotic suscepti-bility,except for intrinsic resistance to co-trimoxazole and ampicillin.These findings highlight L.rhamnosus Lr-CPU2205 as a safe and multi-functional probiotic candidate suitable for application in functional foods and health-promoting formulations.
文摘Antimicrobial resistance(AMR)is a public health problem of global concern.The presence of antibiotic resistance genes(ARGs)in food may aggravate the spread of AMR.Understanding the distribution and transfer of drug-resistant bacteria and ARGs in pickled vegetable foods was critical for food quality and safety.Therefore,this study used metagenomic sequencing,binning technology,and antibiotic susceptibility tests to explore the distribution and transfer of drug-resistant bacteria and ARGs in pickled vegetable foods.The results showed that Bacillota,Lactobacillus,and Salmonella were the dominant bacteria in all samples.A total of 12 ARG types and 52 ARG subtypes were identified.The abundance of bacitracin ARGs in high-salt samples(<2%)was higher,and multidrug ARGs in low-salt samples(7%-10%)was higher.The most abundant ARG subtypes,baca and acrb,were associated with Salmonella.Furthermore,a total of 34 metagenome assembly genomes(MAGs)were obtained by metagenomic binning.MAG8 and MAG25,which carried the largest number of ARGs,virulence factors(VFs),and MGEs,were all derived from Vibrio.More importantly,horizontal gene transfer(HGT)could occur between microorganisms in pickled vegetable samples.In addition,most of the 25 isolates were resistant to bacitracin and multidrug antibiotics.Levilactobacillus brevis PNS3 carried a large number of multidrug ARG genes(patA,macB)and transposable MGE genes(recT,SadA).The results of the drug resistance phenotype were consistent with the results of the drug resistance genotype.These findings suggested that there was a risk of ARGs being carrying and spreading in pickled vegetable foods.
文摘Background Maternal exposure to nitrate, nitrite, and N-nitroso compounds from drinking water or diet has been associated with an increased risk of neural tube defects （NTDs） in some studies. Pickled vegetables contain relatively large amounts of nitrite and N-nitroso compounds. We examined the association between maternal periconceptional consumption of pickled vegetables and NTDs in Shanxi Province of northern China. Methods Data were derived from a population based case-control study of major external birth defects in four counties of $hanxi Province. Participants included 519 NTDs cases identified between 2003 and 2007 and 694 healthy controls. Exposure information was collected within 1 week after delivery. Multivariable non-conditioanal Logistic regression model was used to estimate the adjusted odds ratio （OR） controlling for potential confounding variables.
基金support provided by Agricultural Research Development Agency(Grant no.CRP5507010820)the King Mongkut’s University of Technology Thonburi through the“KMUTT 55th Anniversary Commemorative Fund”(Grant number 60000331).
文摘Salicylic acid(SA)is used as a preservative in food and a biocide in some consumer products.However,in some countries its use is prohibited.The main problem in the electroanalytical determination of SA is the formation of a passive film during the electro-oxidation of SA.To alleviate the passivation during SA measurement,the experiment was conducted by screen-printed carbon electrode(SPCE).While,differential pulse voltammetry(DPV)was essential for oxidation process to provide better sensitivity.Under conditions of optimized pH and electrolyte concentration,we have calibrated SA in the range of 0.001-0.2 mM,with and LOD of 1.6μM.The relative standard deviation of the sensor responses to 40μM and 100μM SA(n=20)was<3%.SA recoveries in samples such as pickled green mustard,pickled bamboo shoots,pickled mango,pickled lime,pickle ginger and apple juice were found to be in the range of 84-113%.
