Picea mongolica is an endemic and endangered species in China. Ecosystem made of Picea mongolica is a special sandy forest ecosystem in China. It is found at ecotone between forest and steppe, or agricultural district...Picea mongolica is an endemic and endangered species in China. Ecosystem made of Picea mongolica is a special sandy forest ecosystem in China. It is found at ecotone between forest and steppe, or agricultural district and pastoral area. Based on investigation, this paper discussed the formation and distribution of Picea mongolica and studied its nature according to ecotone theory. It is clarified that Picea mongolica belongs to Picea meyeri series. That is to say, it became a local race through long-term adaptation to the local climate, then formed allopatric semi-species, and finally turned into a taxonomical species. Picea mongolica forest is a super zonal climax community developing in ecotone between forest zone and steppe zone.展开更多
Tracheid characteristics ofPicea koraiensis from natural stand in Liangshui area and plantation in Mao'ershan area were studied. The results of variance analysis showed that the tracheid length, diameter, and thic...Tracheid characteristics ofPicea koraiensis from natural stand in Liangshui area and plantation in Mao'ershan area were studied. The results of variance analysis showed that the tracheid length, diameter, and thickness of tracheid wall were significant differences between the growth rings. All those characteristics tend to increase from heart-center to bark. In natural stand, it has positive correlation between the characteristics. In plantation, the tracheid length has positive correlation with the trachied diameter, while both the trachied length and the trachied diameter has negative correlation with the thickness of trachied wall. The tracheid length and diameter have no significant difference in growth between natural stand and plantations. The thickness of tracheid wall from the plantation is a little thicker than that from the natural stand before 15 years.展开更多
Picea crassifolia and P.wilsonii,commonly used for afforestation in northern China,are increasingly likely to be subjected to high temperatures and soil drought stress as a result of global warming.However,little is k...Picea crassifolia and P.wilsonii,commonly used for afforestation in northern China,are increasingly likely to be subjected to high temperatures and soil drought stress as a result of global warming.However,little is known about the effects of these stresses on foliar photosynthesis in the two species.To investigate how photosynthetic characteristics and sensitivity respond to prolonged high temperatures and soil drought,foliar gas exchange and other closely related parameters were recorded from four-year-old seedlings of both species.Seedlings were grown under two temperature treatments(25/15 and 35/25 °C) and four soil water regimes [80,60,40 and 20% of maximum field capacity(FC)] for 4 months.Although all treatments significantly reduced photosynthetic rates(Pn) of both species,P.crassifolia exhibited greater photosynthetic acclimation than P.wilsonii.Differences in photosynthetic acclimation were mainly related to variations in stomatal conductance(Cond) and the maximum quantum yield of PSII(Fv/Fm) between treatments.Indeed,higher Cond and Fv/Fmin all treatments were shown for P.crassifolia than for P.wilsonii.Moreover,photosynthesis in P.crassifolia exhibited inherently lower temperature sensitivities(broader span for the temperature response curves; lower b) and higher thermostability(invariable b between treatments).Further,severe drought stress(20% FC) limited the survival of P.wilsonii.Our results indicate that P.wilsonii is more susceptible to high temperatures and soil drought stress.Planting P.crassifolia would be more expected to survive these conditions and hence be of greater benefit to forest stability if predicted increases in drought and temperature in northern China occur.展开更多
Based on two tree-ring maximum latewood density （MXD） chronologies of Picea schrenkiana from the Manas River Basin, Xinjiang, the response characteristics of MXD to climate variation was discussed. Correlation analy...Based on two tree-ring maximum latewood density （MXD） chronologies of Picea schrenkiana from the Manas River Basin, Xinjiang, the response characteristics of MXD to climate variation was discussed. Correlation analysis between MXD chronologies and instrumental records from Shihezi meteorological station showed that each chronology was significantly and positively correlated with the maximum monthly average temperature in July-August, and especially, the regional chronology （RC） was the most highly correlated variable （r=0.54, P〈0.001）. Afterwards, the maximum average temperature in July-August was reconstructed using RC. Comparison among reconstructed temperature, observed values, and the drought index （Is） confirmed that precipitation would affect MXD when the absolute value of Is is greater than 1.5σ （｜Is｜ 〉 2.5 during 1953-2008） or near to 1.5a over a 2-3 year period. The response characteristics are related to the semiarid climate of the study area. In dry years, lack of precipitation would limit the thickening of latewood cell walls and, as a result, impact MXD. Therefore, compared with relatively humid regions, the response of tree-ring MXD to air temperature similarly would be influenced by extreme moisture conditions in semiarid areas, and MXD, as a temperature proxy, should be used prudently on a limited scale.展开更多
[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR ...[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR such as Mg2+, dNTPs, Taq DNA polymerase, template DNA, primers, annealing temperature. [Result] The optimum ISSR-PCR reaction system in 20 μl reaction system was consisted of 1 μl 10×buffer, 1.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 1.0 U Taq DNA polymerase, 40 ng template DNA, 0.6 μmol/L primers. According to gradient test of annealing temperature in optimum ISSR-PCR reaction system of Picea crassifolia kom, it was found that the optimum annealing temperature of UBC 818 was 54.2 ℃ and the annealing temperature was different for different primers.[Conclusion]The construction of ISSR-PCR reaction system provided technical basis for classification of germplasm resources, construction of genetic map, gene mapping of Picea crassifolia kom. through using ISSR technology.展开更多
The spatial distribution of plant populations is an important feature of population structure and it de- termines the population＇s ecological preferences, biological characteristics and relationships with environment...The spatial distribution of plant populations is an important feature of population structure and it de- termines the population＇s ecological preferences, biological characteristics and relationships with environmental factors. The point pattern analysis method was adopted to study the distribution pattern of Picea schrenkiana indi- viduals of different size classes and the correlations between two size classes as well as the impact of topog- raphical attributes on the population distribution. With increasing diameter at breast height, the plant density of the P. schrenkiana population showed a declining trend. Old trees showed a random distribution at a small spatial scale （0-12 m）, whereas saplings, small trees and big trees all had an aggregated distribution at all scales. With the increase of tree age, the scales at which maximal aggregation occurred gradually increased and the aggregation strength decreased. At a small scale （0-16 m）, all size classes showed a negative correlation and the larger the difference between tree size, the more significant the negative correlation. The number of medium, big and old trees had a significantly positive correlation with elevations, whereas the number of saplings and small trees was not significantly correlated with elevations. The numbers of saplings, small and medium trees showed a significant positive correlation with slope gradient, whereas the number of big trees was not significantly correlated, and the number of old trees was negatively correlated with gradient. With the exception of old trees, saplings, small, me- dium and big trees showed negative correlations with convexity index. The study provides a theoretical basis for the conservation, rehabilitation and sustainable management of forest ecosystems in the Tianshan Mountains.展开更多
With Picea koraiensis as the research object which have similar growth vigor in a same habitat, the photosynthetic rate, transpiration rate, chlorophyll content, weight of dry-matter and leaf color change of Picea kor...With Picea koraiensis as the research object which have similar growth vigor in a same habitat, the photosynthetic rate, transpiration rate, chlorophyll content, weight of dry-matter and leaf color change of Picea koraiensis in different lighting conditions were determined. Environment without light sources was taken as the control group. The results showed that different light sources and different distances from light sources to plants had diversified impact on the photosynthetic physiology of Picea koraiensis. Among these different color lights, green light had the least influence on Picea koraiensis, yellow light and purple light had greater influence.展开更多
Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its r...Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.展开更多
基金National Natural Science Foundation of China (39670133 39900019 30070129).
文摘Picea mongolica is an endemic and endangered species in China. Ecosystem made of Picea mongolica is a special sandy forest ecosystem in China. It is found at ecotone between forest and steppe, or agricultural district and pastoral area. Based on investigation, this paper discussed the formation and distribution of Picea mongolica and studied its nature according to ecotone theory. It is clarified that Picea mongolica belongs to Picea meyeri series. That is to say, it became a local race through long-term adaptation to the local climate, then formed allopatric semi-species, and finally turned into a taxonomical species. Picea mongolica forest is a super zonal climax community developing in ecotone between forest zone and steppe zone.
基金part of State 9th Five-Year Plan project of "Early Mensuration of Picea koraiensis (nakai)".
文摘Tracheid characteristics ofPicea koraiensis from natural stand in Liangshui area and plantation in Mao'ershan area were studied. The results of variance analysis showed that the tracheid length, diameter, and thickness of tracheid wall were significant differences between the growth rings. All those characteristics tend to increase from heart-center to bark. In natural stand, it has positive correlation between the characteristics. In plantation, the tracheid length has positive correlation with the trachied diameter, while both the trachied length and the trachied diameter has negative correlation with the thickness of trachied wall. The tracheid length and diameter have no significant difference in growth between natural stand and plantations. The thickness of tracheid wall from the plantation is a little thicker than that from the natural stand before 15 years.
基金supported by the National Natural Science Foundation of China(Grant Nos.31370603,31170571 and31522013)the Fundamental Research Funds for the Central Universities(lzujbky-2016-ct10)
文摘Picea crassifolia and P.wilsonii,commonly used for afforestation in northern China,are increasingly likely to be subjected to high temperatures and soil drought stress as a result of global warming.However,little is known about the effects of these stresses on foliar photosynthesis in the two species.To investigate how photosynthetic characteristics and sensitivity respond to prolonged high temperatures and soil drought,foliar gas exchange and other closely related parameters were recorded from four-year-old seedlings of both species.Seedlings were grown under two temperature treatments(25/15 and 35/25 °C) and four soil water regimes [80,60,40 and 20% of maximum field capacity(FC)] for 4 months.Although all treatments significantly reduced photosynthetic rates(Pn) of both species,P.crassifolia exhibited greater photosynthetic acclimation than P.wilsonii.Differences in photosynthetic acclimation were mainly related to variations in stomatal conductance(Cond) and the maximum quantum yield of PSII(Fv/Fm) between treatments.Indeed,higher Cond and Fv/Fmin all treatments were shown for P.crassifolia than for P.wilsonii.Moreover,photosynthesis in P.crassifolia exhibited inherently lower temperature sensitivities(broader span for the temperature response curves; lower b) and higher thermostability(invariable b between treatments).Further,severe drought stress(20% FC) limited the survival of P.wilsonii.Our results indicate that P.wilsonii is more susceptible to high temperatures and soil drought stress.Planting P.crassifolia would be more expected to survive these conditions and hence be of greater benefit to forest stability if predicted increases in drought and temperature in northern China occur.
基金Supported by Natural Science Foundation of China(41275120,41271120,41301041)Strategic Science and Technology Planning Project of Institute of Geographic Sciences and Natural Resources Research of Chinese Academy of Sciences(2012ZD001)~~
文摘Based on two tree-ring maximum latewood density （MXD） chronologies of Picea schrenkiana from the Manas River Basin, Xinjiang, the response characteristics of MXD to climate variation was discussed. Correlation analysis between MXD chronologies and instrumental records from Shihezi meteorological station showed that each chronology was significantly and positively correlated with the maximum monthly average temperature in July-August, and especially, the regional chronology （RC） was the most highly correlated variable （r=0.54, P〈0.001）. Afterwards, the maximum average temperature in July-August was reconstructed using RC. Comparison among reconstructed temperature, observed values, and the drought index （Is） confirmed that precipitation would affect MXD when the absolute value of Is is greater than 1.5σ （｜Is｜ 〉 2.5 during 1953-2008） or near to 1.5a over a 2-3 year period. The response characteristics are related to the semiarid climate of the study area. In dry years, lack of precipitation would limit the thickening of latewood cell walls and, as a result, impact MXD. Therefore, compared with relatively humid regions, the response of tree-ring MXD to air temperature similarly would be influenced by extreme moisture conditions in semiarid areas, and MXD, as a temperature proxy, should be used prudently on a limited scale.
文摘[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR such as Mg2+, dNTPs, Taq DNA polymerase, template DNA, primers, annealing temperature. [Result] The optimum ISSR-PCR reaction system in 20 μl reaction system was consisted of 1 μl 10×buffer, 1.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 1.0 U Taq DNA polymerase, 40 ng template DNA, 0.6 μmol/L primers. According to gradient test of annealing temperature in optimum ISSR-PCR reaction system of Picea crassifolia kom, it was found that the optimum annealing temperature of UBC 818 was 54.2 ℃ and the annealing temperature was different for different primers.[Conclusion]The construction of ISSR-PCR reaction system provided technical basis for classification of germplasm resources, construction of genetic map, gene mapping of Picea crassifolia kom. through using ISSR technology.
基金funded by the 12th Five-year Science and Technology Support Program(2011BAD38B0505)the Forestry Industry Research Special Funds for Public Welfare Projects (200804022C)the CFERN & GENE Award Funds on Ecological Papers
文摘The spatial distribution of plant populations is an important feature of population structure and it de- termines the population＇s ecological preferences, biological characteristics and relationships with environmental factors. The point pattern analysis method was adopted to study the distribution pattern of Picea schrenkiana indi- viduals of different size classes and the correlations between two size classes as well as the impact of topog- raphical attributes on the population distribution. With increasing diameter at breast height, the plant density of the P. schrenkiana population showed a declining trend. Old trees showed a random distribution at a small spatial scale （0-12 m）, whereas saplings, small trees and big trees all had an aggregated distribution at all scales. With the increase of tree age, the scales at which maximal aggregation occurred gradually increased and the aggregation strength decreased. At a small scale （0-16 m）, all size classes showed a negative correlation and the larger the difference between tree size, the more significant the negative correlation. The number of medium, big and old trees had a significantly positive correlation with elevations, whereas the number of saplings and small trees was not significantly correlated with elevations. The numbers of saplings, small and medium trees showed a significant positive correlation with slope gradient, whereas the number of big trees was not significantly correlated, and the number of old trees was negatively correlated with gradient. With the exception of old trees, saplings, small, me- dium and big trees showed negative correlations with convexity index. The study provides a theoretical basis for the conservation, rehabilitation and sustainable management of forest ecosystems in the Tianshan Mountains.
文摘With Picea koraiensis as the research object which have similar growth vigor in a same habitat, the photosynthetic rate, transpiration rate, chlorophyll content, weight of dry-matter and leaf color change of Picea koraiensis in different lighting conditions were determined. Environment without light sources was taken as the control group. The results showed that different light sources and different distances from light sources to plants had diversified impact on the photosynthetic physiology of Picea koraiensis. Among these different color lights, green light had the least influence on Picea koraiensis, yellow light and purple light had greater influence.
基金supported by the National Natural Science Foundation of China(Grant No.31360086)the grant from the major state basic research development program of china(973 program,No.2013CB429903)
文摘Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.
