Phytomelatonin,an emerging plant hormone,plays vital roles in plant growth,development,and stress adaptation(Arnao et al.,2022;Ullah et al.,2024).It acts both as a direct antioxidant and a signaling molecule,engaging ...Phytomelatonin,an emerging plant hormone,plays vital roles in plant growth,development,and stress adaptation(Arnao et al.,2022;Ullah et al.,2024).It acts both as a direct antioxidant and a signaling molecule,engaging complex networks and interacting with other phytohormones(Liu et al.,2022;Khan et al.,2023).Although phytomelatonin receptors(PMTRs)have been identified in many plants(Wei et al.,2018;Wang et al.,2022;Liu et al.,2025),the downstream signaling mechanisms,particularly receptor-mediated protein modifications and transcriptional regulation,remain poorly characterized.展开更多
Climate change-driven environmental stresses,particularly ultraviolet-B(UV-B)radiation,pose severe threats to grapevine(Vitis vinifera L.)productivity and physiological stability.This study investigated the protective...Climate change-driven environmental stresses,particularly ultraviolet-B(UV-B)radiation,pose severe threats to grapevine(Vitis vinifera L.)productivity and physiological stability.This study investigated the protective role of melatonin in in vitro plantlets of two grapevine cultivars,‘Merlot’and‘Erci¸s’,subjected to low(≈8.25μWcm^(−2),16 h)and high(≈33μW cm^(−2),4 h)UV-B exposure.Significant cultivar-specific responses were observed(p<0.001).The‘Erci¸s’cultivar exhibited higher oxidative stress,with malondialdehyde(MDA)levels reaching 24.30 mmol g^(−1)FW in control plants compared with 14.91±0.25 mmol g^(−1)FW in‘Merlot’.Melatonin provided dose-dependent mitigation,reducing MDA to 12.68 in‘Erci¸s’and 8.52±0.13 in‘Merlot’at 200μmol L^(−1).Antioxidant enzyme activities increased significantly:superoxide dismutase rose from 0.02±0.01 to 0.10 EU g^(−1)in‘Erci¸s’and to 0.13 EU g^(−1)in‘Merlot’,catalase increased up to 0.08 in‘Erci¸s’and 0.16 in‘Merlot’,while ascorbate peroxidase reached 1.06±0.02 and 1.20±0.03,respectively.Pigment traits also improved,with chlorophyll content increasing to 23.70μg cm^(−2)in‘Merlot’and 22.66μg cm^(−2)in‘Erci¸s’,alongside enhanced nitrogen balance index values.Secondary metabolites were elevated,particularly total phenolic content(8.23 GAE 100 g^(−1)in‘Erci¸s’and 5.99 in‘Merlot’)and antioxidant capacity(17.24 and 8.15μmol TE g^(−1),respectively).Correlation analyses revealed strong positive associations betweenmelatonin and antioxidant enzymes(r=0.54-0.85),while principal component analysis explained 64.71%of total variance,separating cultivars and treatments clearly.Clustering patterns showed distinct grouping of enzymatic defenses,phenolic compounds,and pigments,reflecting coordinated protective mechanisms.Overall,melatonin application,especially at 200μmol L^(−1),significantly enhanced enzymatic and non-enzymatic antioxidant defenses,stabilized photosynthetic pigments,and reduced oxidative damage,with stronger protective efficiency in‘Merlot’.The research provided valuable insights for developing biotechnological approaches to enhance grape stress tolerance in the context of climate change challenges.展开更多
Melatonin is a conserved pleiotropic molecule in animals and plants.Melatonin is involved in many development processes and stress responses;thus,exploring its function in plants,particularly in horticultural plants,h...Melatonin is a conserved pleiotropic molecule in animals and plants.Melatonin is involved in many development processes and stress responses;thus,exploring its function in plants,particularly in horticultural plants,has become a rapidly developing field.Many studies have revealed that phytomelatonin acts as a plant biostimulant and increase its tolerance to various abiotic stressors,including extreme temperature,drought,osmotic disturbance,heavy metals,and ultraviolet(UV).Melatonin appears to have roles in the scavenging of reactive oxygen species(ROS)and other free radicals,affecting the primary and secondary metabolism of plants,regulating the transcripts of stress-related enzymes and transcription factors,and crosstalk with other hormones under different environmental conditions.This pleiotropy makes phytomelatonin an attractive regulator to improve resistance to abiotic stress in plants.The recent discovery of the potential phytomelatonin receptor CAND2/PMTR1 and the proposition of putative models related to the phytomelatonin signaling pathways makes phytomelatonin a new plant hormone.Based on relevant studies from our laboratory,this review summarizes the phytomelatonin biosynthetic and metabolic pathways in plants and the latest research progress on phytomelatonin in abiotic stress of horticultural plants.This study will provide a reference for elucidating the regulatory mechanism of phytomelatonin affecting the resistance to abiotic stress in plants.展开更多
基金supported by the grants from the Key Research and Development Program of Xinjiang Uygur autonomous region in China(Grant No.2023B02017)the National Key Research and Development Program of China(Grant No.2024YFD2300703)+1 种基金the financial support from the Beijing Rural Revitalization Agricultural Science and Technology Project(Grant No.NY2401080000),BAIC01-2025the 2115 Talent Development Program of China Agricultural University.
文摘Phytomelatonin,an emerging plant hormone,plays vital roles in plant growth,development,and stress adaptation(Arnao et al.,2022;Ullah et al.,2024).It acts both as a direct antioxidant and a signaling molecule,engaging complex networks and interacting with other phytohormones(Liu et al.,2022;Khan et al.,2023).Although phytomelatonin receptors(PMTRs)have been identified in many plants(Wei et al.,2018;Wang et al.,2022;Liu et al.,2025),the downstream signaling mechanisms,particularly receptor-mediated protein modifications and transcriptional regulation,remain poorly characterized.
基金funded by Van Yuzuncu Yıl University Scientific Research Project Department(Project No.FYL-2021-9577).
文摘Climate change-driven environmental stresses,particularly ultraviolet-B(UV-B)radiation,pose severe threats to grapevine(Vitis vinifera L.)productivity and physiological stability.This study investigated the protective role of melatonin in in vitro plantlets of two grapevine cultivars,‘Merlot’and‘Erci¸s’,subjected to low(≈8.25μWcm^(−2),16 h)and high(≈33μW cm^(−2),4 h)UV-B exposure.Significant cultivar-specific responses were observed(p<0.001).The‘Erci¸s’cultivar exhibited higher oxidative stress,with malondialdehyde(MDA)levels reaching 24.30 mmol g^(−1)FW in control plants compared with 14.91±0.25 mmol g^(−1)FW in‘Merlot’.Melatonin provided dose-dependent mitigation,reducing MDA to 12.68 in‘Erci¸s’and 8.52±0.13 in‘Merlot’at 200μmol L^(−1).Antioxidant enzyme activities increased significantly:superoxide dismutase rose from 0.02±0.01 to 0.10 EU g^(−1)in‘Erci¸s’and to 0.13 EU g^(−1)in‘Merlot’,catalase increased up to 0.08 in‘Erci¸s’and 0.16 in‘Merlot’,while ascorbate peroxidase reached 1.06±0.02 and 1.20±0.03,respectively.Pigment traits also improved,with chlorophyll content increasing to 23.70μg cm^(−2)in‘Merlot’and 22.66μg cm^(−2)in‘Erci¸s’,alongside enhanced nitrogen balance index values.Secondary metabolites were elevated,particularly total phenolic content(8.23 GAE 100 g^(−1)in‘Erci¸s’and 5.99 in‘Merlot’)and antioxidant capacity(17.24 and 8.15μmol TE g^(−1),respectively).Correlation analyses revealed strong positive associations betweenmelatonin and antioxidant enzymes(r=0.54-0.85),while principal component analysis explained 64.71%of total variance,separating cultivars and treatments clearly.Clustering patterns showed distinct grouping of enzymatic defenses,phenolic compounds,and pigments,reflecting coordinated protective mechanisms.Overall,melatonin application,especially at 200μmol L^(−1),significantly enhanced enzymatic and non-enzymatic antioxidant defenses,stabilized photosynthetic pigments,and reduced oxidative damage,with stronger protective efficiency in‘Merlot’.The research provided valuable insights for developing biotechnological approaches to enhance grape stress tolerance in the context of climate change challenges.
基金supported by the grants from National Natural Science Foundation of China(Grant Nos.32172598,32172599)Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(Grant No.320LH013)+1 种基金the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(Grant No.CEFF-PXM2019-014207-000032)Beijing Agriculture Innovation Consortium(Grant No.BAIC01-2024).
文摘Melatonin is a conserved pleiotropic molecule in animals and plants.Melatonin is involved in many development processes and stress responses;thus,exploring its function in plants,particularly in horticultural plants,has become a rapidly developing field.Many studies have revealed that phytomelatonin acts as a plant biostimulant and increase its tolerance to various abiotic stressors,including extreme temperature,drought,osmotic disturbance,heavy metals,and ultraviolet(UV).Melatonin appears to have roles in the scavenging of reactive oxygen species(ROS)and other free radicals,affecting the primary and secondary metabolism of plants,regulating the transcripts of stress-related enzymes and transcription factors,and crosstalk with other hormones under different environmental conditions.This pleiotropy makes phytomelatonin an attractive regulator to improve resistance to abiotic stress in plants.The recent discovery of the potential phytomelatonin receptor CAND2/PMTR1 and the proposition of putative models related to the phytomelatonin signaling pathways makes phytomelatonin a new plant hormone.Based on relevant studies from our laboratory,this review summarizes the phytomelatonin biosynthetic and metabolic pathways in plants and the latest research progress on phytomelatonin in abiotic stress of horticultural plants.This study will provide a reference for elucidating the regulatory mechanism of phytomelatonin affecting the resistance to abiotic stress in plants.
