Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among...Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among 36 mutant lines using morpho-physiological indices.Seedlings of mutant lines and check varieties were grown under both normal(control)and heat-stress conditions in growth chambers.Data were recorded on root-shoot parameters(length,fresh weight,dry weight,and ratio),relative water content(RWC),stability of cell membrane,pigment content,and chlorophyll fluorescence.Two-way analysis of variance showed significant(p<0.01,p<0.001)variation among 15 morpho-physiological features in both growing conditions.Correlation studies showed that pigment-specific indices(total chl,chl a,chl b,carotenoid,Fv/Fm—chlorophyll fluorescence)had significant positive correlations with rootshoot fresh weight(RFW,SFW)and root-shoot dry weight(RDW,SDW),and negative association with relative water content,stability of cell membrane at both conditions.Hierarchical cluster analysis revealed three primary clusters where cluster III(18 genotypes)showed minimal changes in studied characters than cluster I(11 genotypes)and cluster II(13 genotypes).Results obtained from this study revealed that the identified heat-sensitive(30-2 SSH)and heat-tolerant genotypes(27-2(4),29-2(2),and 30-2 SSH)of wheat might provide useful breeding materials for enhancing wheat yield even more in this period of uncertain climate change.展开更多
The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness...The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.展开更多
Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives wer...Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.展开更多
Hybridization is a driving force in ecological transitions and speciation,yet direct evidence linking it to adaptive differentiation in natural systems remains limited.This study evaluates the role of hybridization in...Hybridization is a driving force in ecological transitions and speciation,yet direct evidence linking it to adaptive differentiation in natural systems remains limited.This study evaluates the role of hybridization in the speciation of Pinus densata,a keystone forest species on the southeastern Tibetan Plateau.By creating artificialinterspecificF1s and a long-term common garden experiment on the plateau,we provide in situ assessments on 44 growth and physiological traits across four seasons,along with RNA sequencing.We found significantphenotypic divergence between P.densata and its putative parental species P.tabuliformis and P.yunnanensis,with P.densata demonstrating superior growth and dynamic balance between photosynthesis and photoprotection.The F1s closely resembled P.densata in most traits.Gene expression revealed 19%–10%of 34,000 examined genes as differentially expressed in P.densata and F1s relative to mid-parent expression values.Both additive(4%)and non-additive gene actions(5%–6%in F1s,10%–12%in P.densata)were common,while transgressive expression occurred more frequently in the stabilized natural hybrids,illustrating transcriptomic reprogramming brought by hybridization and further divergence by natural selection.We provide compelling evidence for hybridization-derived phenotypic divergence at both physiological and gene expression levels that could have contributed to the adaptation of P.densata to high plateau habitat where both parental species have low fitness.The altered physiology and gene expression in hybrids serve both as a substrate for novel ecological adaptation and as a mechanism for the initiation of reproductive isolation.展开更多
Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A random...Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.展开更多
Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In...Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.展开更多
Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves t...Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves through various ways,so that people can actively change their own physiological changes when they perceive the changes in some physiological functions of their own,and consciously control the physiological functions of various aspects of the autonomic nervous system within a fixed range.It can play a role in improving physical health status,preventing and treating some somatic diseases.Studies have shown that biofeedback relaxation training can make the training subjects learn to regulate their own physiological functions and psychological activities,improve negative emotional states such as depression and anxiety by regulating the body function,and can also be used to assist in the treatment of other clinical diseases.As a new psychological intervention,biofeedback relaxation training has great development potential and can be better applied to the prevention and treatment of physical and mental diseases in the general population in the future.展开更多
Protein Kinase G(PKG)is an important intracellular signal transduction enzyme,and its activity is modulated by cyclic guanosine monophosphate(cGMP).PKG plays a pivotal role in various significant physiological process...Protein Kinase G(PKG)is an important intracellular signal transduction enzyme,and its activity is modulated by cyclic guanosine monophosphate(cGMP).PKG plays a pivotal role in various significant physiological processes,including vascular smooth muscle relaxation,myocardial cell function regulation,neuron growth,and synaptic plasticity,et al.In recent years,the role of PKG in diseases has gradually attracted attention,and the abnormalities in its signaling pathway are closely related to the occurrence and development of cardiovascular and neurological diseases.Although PKG has been widely studied,its complex functions in different physiological systems and potential innovative applications still need to be further explored.This article reviews the purification techniques for PKG,discusses the advantages and disadvantages of different extraction methods,summarizes the structure and activation mechanism of each domain of PKG,and analyzes the physiological functions of PKG in organisms,especially the well-established roles in the cardiovascular system,nervous system,and endocrine system.The emerging therapeutic applications of PKG are also reviewed.In addition,the challenges of this field are proposed at the end.展开更多
Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negate...Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.展开更多
Salinity stress is a major challenge for global agriculture,particularly in arid and semi-arid regions,limiting plant productivity due to water and soil salinity.These conditions particularly affect countries along th...Salinity stress is a major challenge for global agriculture,particularly in arid and semi-arid regions,limiting plant productivity due to water and soil salinity.These conditions particularly affect countries along the southern Mediterranean rim,including Algeria,which primarily focuses on pastoral and forage practices.This study investigates salinity tolerance and ecotypic variability in Vicia narbonensis L.,a fodder legume species recognized for its potential to reclaim marginal soils.Morphological,physiological,and biochemical responses were assessed in three ecotypes(eco2,eco9,and eco10)exposed to different salinity levels(low,moderate,and severe).The study was conducted using a completely randomized block design with three blocks per ecotype per dose.The results from the two-way analysis of variance demonstrate significant effects across nearly all attributes studied,revealing distinct ecotypic responses.These findings underscore variations in growth parameters,osmotic regulation mechanisms,and biochemical adjustments.The substantial diversity observed among these ecotypes in their response to salinity provides valuable insights for breeders addressing both agronomic and ecological challenges.Multivariate analyses,including Principal Component Analysis(PCA),revealed key variables distinguishing between ecotypes under salinity stress.Moreover,Classification based on Salinity Tolerance Indices(STI)further differentiated ecotypic performance with more precision,and this is because of the combination of the different parameters studied.These results open up new prospects for the development of strategies to improve the salinity tolerance of forage legumes.展开更多
Objective This is a self-controlled multicenter retrospective study based on the clinical efficacy and complications of physiological reconstruction in the treatment of moderate and severe pelvic organ prolapse.Method...Objective This is a self-controlled multicenter retrospective study based on the clinical efficacy and complications of physiological reconstruction in the treatment of moderate and severe pelvic organ prolapse.Methods From December 2014 to August 2021,517 women were included and registered for physiological reconstruction at four Chinese urogynecology institutions.We enrolled 364 women with POP-Q stage≥3.The degree of POP was quantified via a POP-Q system.The surgical purpose of physiological reconstruction is to repair the vagina,levator ani muscle,perineum,and urogenital hiatus and adopt a repair method in accordance with the axial direction of physiology.All 330 evaluable participants were followed for 2 years.The evaluation indices included the PFDI-20,PGI-I,PFIQ-7,PISQ-12,PGI-I,and PGI-S.All complications were coded according to the category-time-site system proposed by the International Urogynecological Association(IUGA)and International Continence Society(ICS).Results Compared with the preoperative POP-Q scores,statistically significant improvements were observed at the 6-month,1-year and 2-year time points(P<0.001).Statistically significant improvements in quality of life were observed across all time points.Conclusions Physiologic reconstructive surgical techniques combined with modified anterior pelvic floor mesh implantation could help restore the physiologic axis and vaginal shape,which may be the most important factors in maintaining the functional position of pelvic floor organs and is the most effective method for repairing the pelvic fascia tendon arch.This surgical method is safe,feasible,and effective in patients with severe prolapse.展开更多
Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping...Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping is mainly attributed to the large amount of purchased resources such as water and fertilizer,plastic film,and mechanical power.These lead to a decline in cultivated land quality and exacerbate intercrops'premature root and canopy senescence.So,the application of traditional intercropping faces major challenges in crop production.This paper analyzes the manifestations,occurrence mechanisms,and agronomic regulatory pathways of crop senescence.The physiological and ecological characteristics of intercropping to delay root and canopy senescence of crops are reviewed in this paper.The main agronomic regulatory pathways of intercropping to delay root and canopy senescence of crops are based on above-and blow-ground interactions,including collocation of crop varieties,spatial arrangement,water and fertilizer management,and tillage and mulch practices.Future research fields of intercropping to delay root and canopy senescence should focus on the aspects of selecting and breeding special varieties,application of molecular biology techniques,and developing or applying models to predict and evaluate the root and canopy senescence process of intercrops.Comprehensive analysis and evaluation of different research results could provide a basis for enhancing intercropping delay root and canopy senescence through adopting innovative technologies for regulating the physio-ecological characteristics of intercrops.This would support developing and adopting high-yield,efficient,and sustainable intercropping systems in arid and semi-arid areas with high population density,limited land,and abundant light and heat resources.展开更多
[Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ...[Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ultraviolet irradiation in short time. [Method] The winter wheat taken as materials were treated with 15 and 30 pW/cm^2 UV-B radiation, then the physiological indexes such as pigment content and photosynthetic rate were determined. [ Results] The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 pW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UVB treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2 h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious. [ Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose. With the increase of treatment time, the damage was alleviated; besides, the result was not similar to that of UV-B radiation in long time.展开更多
The ion levels in the epidermal bulliform cells of epidermis, mesophyll cells and cells of the vascular bundle sheath of four ecotypes of reed (Phragmites australis (Cav.) Trin. ex Steud.) were determined by means of ...The ion levels in the epidermal bulliform cells of epidermis, mesophyll cells and cells of the vascular bundle sheath of four ecotypes of reed (Phragmites australis (Cav.) Trin. ex Steud.) were determined by means of X_ray microanalysis. The results indicated that higher K +, Na +, Ca 2+ , Mg 2+ and Cl - were distributed in the vascular bundle sheath cells than in mesophyll cells and epidermal bulliform cells of the swamp ecotype. Higher Ca 2+ was found in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, higher Mg 2+ in the mesophyll cells and higher K +, Na + and Cl - in the vascular bundle sheath cells of the dune ecotype. Higher Na + and Mg 2+ was determined in the mesophyll cells than in the bulliform cells and vascular bundle sheath cells, and higher K +, Ca 2+ and Cl - in the vascular bundle sheath cells of the light salt meadow ecotype. In the heavy salt meadow ecotype, higher Na + and Mg 2+ were accumulated in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, but higher K +, Ca 2+ and Cl - in the mesophyll cells. Furthermore, the distributional conditions of the above five ions in leaf cells of the four ecotypes and their significance in the physiological adaptation of reed to habitat were discussed in detail.展开更多
[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experiment...[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last.展开更多
[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napu...[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde （MDA）, soluble sugar, soluble protein, and the activities of superoxide dismutase （SOD）, catalase（CAT）, peroxidase（POD） were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out： YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.展开更多
[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigo...[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigor index,peroxidase（POD） activity,superoxide dismutase（SOD） activity and malondialdehyde（MDA） content for four sunflower germplasms such as＇SunM20＇,＇Deep Purple Minle＇,＇Da San Kui Hua 4＇and＇Ji Kui 24＇were studied under high temperature and high humidity conditions（100% RH,45 ℃） for different days（0 d,2 d,4 d,6 d,8 d,10 d）.[Result]The result showed that the germination capacity,vigor of germination,germination index,vigor index,POD activity,SOD activity declined gradually with the increase of seed aging days,whereas MDA content enhanced by degrees;The diggerences of resistance to artifical aging existed among the four accessions,＇SunM20＇was the most resistant one,and exhibited the strongest seed vigor,highest activities of two protective enzyme（POD,SOD） and lowest content of MDA at the uniform condition,moreover,the seed vigor and protective enzyme activities of＇SunM20＇changed slowest among the four materials during the aging process.[Conclusion]The distinct reduction of POD,SOD activities maybe the main reasons for the decrease of sunflower seed vigor at the artificial aging,and the gradual accumulation of a few MDA accelerated seeds aging.展开更多
A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being high...A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase （SOD） activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde （MDA） content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.展开更多
[Objective] The paper was to study the effect of low temperature stress on physiological and biochemical characteristics of Podocarpus nagi. [Method] Through the determination of physiological indices such as plasma m...[Objective] The paper was to study the effect of low temperature stress on physiological and biochemical characteristics of Podocarpus nagi. [Method] Through the determination of physiological indices such as plasma membrane permeability, free proline content, soluble sugar content, malondialdehyde （MDA） content and chlorophyll content, the change law of these indices of P. nagi seedlings under natural conditions and artificially controlled low temperature conditions within the continuous 5 d was studied. [Result] The soluble sugar content, free proline content, MDA content and plasma membrane permeability of P. nagi seedlings were slightly in- creased compared with control; the chlorophyll content gradually decreased with the prolongation of low temperature stress. P. nagi seedlings produced active response to low temperature stress, so the low temperature injury P. nagi seedlings suffered was reduced. [Conclusion] The study provided theoretical basis for winter management in P. nagi cultivation in Hanjiang plain area.展开更多
[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects...[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments：remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system.展开更多
文摘Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among 36 mutant lines using morpho-physiological indices.Seedlings of mutant lines and check varieties were grown under both normal(control)and heat-stress conditions in growth chambers.Data were recorded on root-shoot parameters(length,fresh weight,dry weight,and ratio),relative water content(RWC),stability of cell membrane,pigment content,and chlorophyll fluorescence.Two-way analysis of variance showed significant(p<0.01,p<0.001)variation among 15 morpho-physiological features in both growing conditions.Correlation studies showed that pigment-specific indices(total chl,chl a,chl b,carotenoid,Fv/Fm—chlorophyll fluorescence)had significant positive correlations with rootshoot fresh weight(RFW,SFW)and root-shoot dry weight(RDW,SDW),and negative association with relative water content,stability of cell membrane at both conditions.Hierarchical cluster analysis revealed three primary clusters where cluster III(18 genotypes)showed minimal changes in studied characters than cluster I(11 genotypes)and cluster II(13 genotypes).Results obtained from this study revealed that the identified heat-sensitive(30-2 SSH)and heat-tolerant genotypes(27-2(4),29-2(2),and 30-2 SSH)of wheat might provide useful breeding materials for enhancing wheat yield even more in this period of uncertain climate change.
基金Supported by National Natural Science Foundation of China(Grant Nos.52375279,52175001)Beijing Natural Science Foundation(Grant No.3252002).
文摘The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.
基金funded by the‘National Key R&D Program Subject of China’(No.2021YFD1100301)the post subsidy project of National Key R&D Program,and the Guizhou Modern Agriculture Research System(GZMARS)-Plateau characteristic vegetable industry.
文摘Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.
基金supported by the National Natural Science Foundation of China(32171816)T4F program Sweden.
文摘Hybridization is a driving force in ecological transitions and speciation,yet direct evidence linking it to adaptive differentiation in natural systems remains limited.This study evaluates the role of hybridization in the speciation of Pinus densata,a keystone forest species on the southeastern Tibetan Plateau.By creating artificialinterspecificF1s and a long-term common garden experiment on the plateau,we provide in situ assessments on 44 growth and physiological traits across four seasons,along with RNA sequencing.We found significantphenotypic divergence between P.densata and its putative parental species P.tabuliformis and P.yunnanensis,with P.densata demonstrating superior growth and dynamic balance between photosynthesis and photoprotection.The F1s closely resembled P.densata in most traits.Gene expression revealed 19%–10%of 34,000 examined genes as differentially expressed in P.densata and F1s relative to mid-parent expression values.Both additive(4%)and non-additive gene actions(5%–6%in F1s,10%–12%in P.densata)were common,while transgressive expression occurred more frequently in the stabilized natural hybrids,illustrating transcriptomic reprogramming brought by hybridization and further divergence by natural selection.We provide compelling evidence for hybridization-derived phenotypic divergence at both physiological and gene expression levels that could have contributed to the adaptation of P.densata to high plateau habitat where both parental species have low fitness.The altered physiology and gene expression in hybrids serve both as a substrate for novel ecological adaptation and as a mechanism for the initiation of reproductive isolation.
文摘Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.
基金supported by the National Natural Science Foundation of China(52127801)Postdoctoral Fellowship Program of CPSF under Grant Number GZC20231545,China Postdoctoral Science Foundation(2024T170557 and 2023M742224)+1 种基金Shanghai Post-doctoral Excellence Program(No.2023440)City University of Hong Kong Donation Grants(DON-RMG No.9229021 and 9220061).
文摘Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.
基金The Basic Scientific Research Project of Colleges and Universities in 2022:HRV and Brain Electrophysiological Mechanism of Electromyography Biofeedback in Improving Depression and Anxiety of College Students(Project No.:LJKMZ20221274)。
文摘Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves through various ways,so that people can actively change their own physiological changes when they perceive the changes in some physiological functions of their own,and consciously control the physiological functions of various aspects of the autonomic nervous system within a fixed range.It can play a role in improving physical health status,preventing and treating some somatic diseases.Studies have shown that biofeedback relaxation training can make the training subjects learn to regulate their own physiological functions and psychological activities,improve negative emotional states such as depression and anxiety by regulating the body function,and can also be used to assist in the treatment of other clinical diseases.As a new psychological intervention,biofeedback relaxation training has great development potential and can be better applied to the prevention and treatment of physical and mental diseases in the general population in the future.
基金supported by the National Natural Science Foundation of China(Nos.22374033,22174031,22407037)the Natural Science Foundation of Heilongjiang Province(No.ZD2022B001).
文摘Protein Kinase G(PKG)is an important intracellular signal transduction enzyme,and its activity is modulated by cyclic guanosine monophosphate(cGMP).PKG plays a pivotal role in various significant physiological processes,including vascular smooth muscle relaxation,myocardial cell function regulation,neuron growth,and synaptic plasticity,et al.In recent years,the role of PKG in diseases has gradually attracted attention,and the abnormalities in its signaling pathway are closely related to the occurrence and development of cardiovascular and neurological diseases.Although PKG has been widely studied,its complex functions in different physiological systems and potential innovative applications still need to be further explored.This article reviews the purification techniques for PKG,discusses the advantages and disadvantages of different extraction methods,summarizes the structure and activation mechanism of each domain of PKG,and analyzes the physiological functions of PKG in organisms,especially the well-established roles in the cardiovascular system,nervous system,and endocrine system.The emerging therapeutic applications of PKG are also reviewed.In addition,the challenges of this field are proposed at the end.
基金funded by the National Natural Science Foundation of China(Grant No.32201896)the Zhejiang Province Key Research and Development Plan Project,China(Grant No.2022C02034)the National Modern Agricultural Industrial Technology System Construction Project,China(Grant No.CARS-01-21).
文摘Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.
基金Direction Generale de la Recherche Scientifique et du Developpement Technologique(DGRSDT)Algeria,and the Researchers Supporting Project No.(RSP2025R390),King Saud University,Riyadh,Saudi Arabia.
文摘Salinity stress is a major challenge for global agriculture,particularly in arid and semi-arid regions,limiting plant productivity due to water and soil salinity.These conditions particularly affect countries along the southern Mediterranean rim,including Algeria,which primarily focuses on pastoral and forage practices.This study investigates salinity tolerance and ecotypic variability in Vicia narbonensis L.,a fodder legume species recognized for its potential to reclaim marginal soils.Morphological,physiological,and biochemical responses were assessed in three ecotypes(eco2,eco9,and eco10)exposed to different salinity levels(low,moderate,and severe).The study was conducted using a completely randomized block design with three blocks per ecotype per dose.The results from the two-way analysis of variance demonstrate significant effects across nearly all attributes studied,revealing distinct ecotypic responses.These findings underscore variations in growth parameters,osmotic regulation mechanisms,and biochemical adjustments.The substantial diversity observed among these ecotypes in their response to salinity provides valuable insights for breeders addressing both agronomic and ecological challenges.Multivariate analyses,including Principal Component Analysis(PCA),revealed key variables distinguishing between ecotypes under salinity stress.Moreover,Classification based on Salinity Tolerance Indices(STI)further differentiated ecotypic performance with more precision,and this is because of the combination of the different parameters studied.These results open up new prospects for the development of strategies to improve the salinity tolerance of forage legumes.
基金supported by the National Natural Science Foundation of China(No.82260297)Yunnan Province Clinical Research Center for Chronic Kidney Disease(No.202102AA100060).
文摘Objective This is a self-controlled multicenter retrospective study based on the clinical efficacy and complications of physiological reconstruction in the treatment of moderate and severe pelvic organ prolapse.Methods From December 2014 to August 2021,517 women were included and registered for physiological reconstruction at four Chinese urogynecology institutions.We enrolled 364 women with POP-Q stage≥3.The degree of POP was quantified via a POP-Q system.The surgical purpose of physiological reconstruction is to repair the vagina,levator ani muscle,perineum,and urogenital hiatus and adopt a repair method in accordance with the axial direction of physiology.All 330 evaluable participants were followed for 2 years.The evaluation indices included the PFDI-20,PGI-I,PFIQ-7,PISQ-12,PGI-I,and PGI-S.All complications were coded according to the category-time-site system proposed by the International Urogynecological Association(IUGA)and International Continence Society(ICS).Results Compared with the preoperative POP-Q scores,statistically significant improvements were observed at the 6-month,1-year and 2-year time points(P<0.001).Statistically significant improvements in quality of life were observed across all time points.Conclusions Physiologic reconstructive surgical techniques combined with modified anterior pelvic floor mesh implantation could help restore the physiologic axis and vaginal shape,which may be the most important factors in maintaining the functional position of pelvic floor organs and is the most effective method for repairing the pelvic fascia tendon arch.This surgical method is safe,feasible,and effective in patients with severe prolapse.
基金supported by the National Natural Science Foundation of China(32101857 and U21A20218)the China Agricultural University Corresponding Support Research Joint Fund(GSAU-DKZY-2024-001)+1 种基金the Science and Technology Program in Gansu Province,China(24ZDNA008and23JRRA1407)the Fuxi Young Talents Fund of Gansu Agricultural University,China(Gaufx-03Y10).
文摘Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping is mainly attributed to the large amount of purchased resources such as water and fertilizer,plastic film,and mechanical power.These lead to a decline in cultivated land quality and exacerbate intercrops'premature root and canopy senescence.So,the application of traditional intercropping faces major challenges in crop production.This paper analyzes the manifestations,occurrence mechanisms,and agronomic regulatory pathways of crop senescence.The physiological and ecological characteristics of intercropping to delay root and canopy senescence of crops are reviewed in this paper.The main agronomic regulatory pathways of intercropping to delay root and canopy senescence of crops are based on above-and blow-ground interactions,including collocation of crop varieties,spatial arrangement,water and fertilizer management,and tillage and mulch practices.Future research fields of intercropping to delay root and canopy senescence should focus on the aspects of selecting and breeding special varieties,application of molecular biology techniques,and developing or applying models to predict and evaluate the root and canopy senescence process of intercrops.Comprehensive analysis and evaluation of different research results could provide a basis for enhancing intercropping delay root and canopy senescence through adopting innovative technologies for regulating the physio-ecological characteristics of intercrops.This would support developing and adopting high-yield,efficient,and sustainable intercropping systems in arid and semi-arid areas with high population density,limited land,and abundant light and heat resources.
基金Supported by Natural Science Foundation of China(Grant:30771156)Student Technology New Ideas Project of Liaocheng University(Grant:SRT08111SM2)~~
文摘[Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ultraviolet irradiation in short time. [Method] The winter wheat taken as materials were treated with 15 and 30 pW/cm^2 UV-B radiation, then the physiological indexes such as pigment content and photosynthetic rate were determined. [ Results] The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 pW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UVB treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2 h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious. [ Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose. With the increase of treatment time, the damage was alleviated; besides, the result was not similar to that of UV-B radiation in long time.
文摘The ion levels in the epidermal bulliform cells of epidermis, mesophyll cells and cells of the vascular bundle sheath of four ecotypes of reed (Phragmites australis (Cav.) Trin. ex Steud.) were determined by means of X_ray microanalysis. The results indicated that higher K +, Na +, Ca 2+ , Mg 2+ and Cl - were distributed in the vascular bundle sheath cells than in mesophyll cells and epidermal bulliform cells of the swamp ecotype. Higher Ca 2+ was found in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, higher Mg 2+ in the mesophyll cells and higher K +, Na + and Cl - in the vascular bundle sheath cells of the dune ecotype. Higher Na + and Mg 2+ was determined in the mesophyll cells than in the bulliform cells and vascular bundle sheath cells, and higher K +, Ca 2+ and Cl - in the vascular bundle sheath cells of the light salt meadow ecotype. In the heavy salt meadow ecotype, higher Na + and Mg 2+ were accumulated in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, but higher K +, Ca 2+ and Cl - in the mesophyll cells. Furthermore, the distributional conditions of the above five ions in leaf cells of the four ecotypes and their significance in the physiological adaptation of reed to habitat were discussed in detail.
基金Supported by Program for New Century Excellent Talents in University(NCET-04-0907)the Innovative Research Team in University (IRT0453)~~
文摘[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last.
基金Supported by Rapeseed Industry Construction Program of Department of Agriculture of Yunnan ProvinceFund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~
文摘[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde （MDA）, soluble sugar, soluble protein, and the activities of superoxide dismutase （SOD）, catalase（CAT）, peroxidase（POD） were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out： YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.
文摘[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigor index,peroxidase（POD） activity,superoxide dismutase（SOD） activity and malondialdehyde（MDA） content for four sunflower germplasms such as＇SunM20＇,＇Deep Purple Minle＇,＇Da San Kui Hua 4＇and＇Ji Kui 24＇were studied under high temperature and high humidity conditions（100% RH,45 ℃） for different days（0 d,2 d,4 d,6 d,8 d,10 d）.[Result]The result showed that the germination capacity,vigor of germination,germination index,vigor index,POD activity,SOD activity declined gradually with the increase of seed aging days,whereas MDA content enhanced by degrees;The diggerences of resistance to artifical aging existed among the four accessions,＇SunM20＇was the most resistant one,and exhibited the strongest seed vigor,highest activities of two protective enzyme（POD,SOD） and lowest content of MDA at the uniform condition,moreover,the seed vigor and protective enzyme activities of＇SunM20＇changed slowest among the four materials during the aging process.[Conclusion]The distinct reduction of POD,SOD activities maybe the main reasons for the decrease of sunflower seed vigor at the artificial aging,and the gradual accumulation of a few MDA accelerated seeds aging.
文摘A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase （SOD） activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde （MDA） content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.
基金Supported by Doctoral Starting Fund of Yangtze University(801190010105)~~
文摘[Objective] The paper was to study the effect of low temperature stress on physiological and biochemical characteristics of Podocarpus nagi. [Method] Through the determination of physiological indices such as plasma membrane permeability, free proline content, soluble sugar content, malondialdehyde （MDA） content and chlorophyll content, the change law of these indices of P. nagi seedlings under natural conditions and artificially controlled low temperature conditions within the continuous 5 d was studied. [Result] The soluble sugar content, free proline content, MDA content and plasma membrane permeability of P. nagi seedlings were slightly in- creased compared with control; the chlorophyll content gradually decreased with the prolongation of low temperature stress. P. nagi seedlings produced active response to low temperature stress, so the low temperature injury P. nagi seedlings suffered was reduced. [Conclusion] The study provided theoretical basis for winter management in P. nagi cultivation in Hanjiang plain area.
基金Supported by Natural Science Foundation of Fujian Province(2008J0052 )Technology Program in Xiamen City(3502Z20072007)~~
文摘[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments：remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system.
