The large yellow croaker(Larimichthys crocea)is a flagship marine fish in China given its extreme commercial value and golden-yellow coloration.However,the genetic mechanisms underlying golden-yellow coloration remain...The large yellow croaker(Larimichthys crocea)is a flagship marine fish in China given its extreme commercial value and golden-yellow coloration.However,the genetic mechanisms underlying golden-yellow coloration remain unclear.Here,we construct a telomere-to-telomere gap-free genome assembly(T2TLarcro_1.0)spanning 716.87 Mb,with a contig N50 of 31.75 Mb.Compared to the current reference genome(L_crocea_2.0),T2T-Larcro_1.0 incorporates 112.70 Mb of previously unassembled regions and 2368 newly anchored genes.This assembly facilitates comparative genomics analyses in sciaenids by identifying several candidate genes(e.g.,OPNVA,nNOS,RDH13)potentially involved in evolution of golden-yellow coloration.Transcriptomic analyses further confirm expression of OPNVA-encoded vertebrate ancient opsin(VA opsin)in skin tissues of the large yellow croaker,suggesting its role as an extraretinal photoreceptor regulating localized golden-yellow coloration.Integrating genomics and transcriptomics results,we uncover the triggering effect of VA opsin linking skin and neural photoreception to physiological regulation of body color change(golden-yellow to silvery-white)in L.crocea.Collectively,our findings provide molecular evidence that elucidate the underlying evolutionary mechanism of goldenyellow coloration in L.crocea.This high-quality genome assembly also serves as an improved resource for biological evolution,genetic improvement,and selective breeding of L.crocea.展开更多
Vision and photoentrainment in fishes are vital for feeding,avoiding predation,spatial orientation,navigation,social communication and the synchronization of many homeostatic functions such as activity patterns and sl...Vision and photoentrainment in fishes are vital for feeding,avoiding predation,spatial orientation,navigation,social communication and the synchronization of many homeostatic functions such as activity patterns and sleep.The camera-like(image-forming)eyes of fishes are optimized to provide a clear view of their preferred ecological niche,while non-visual photoreceptors provide irradiance detection that mediates circadian photoentrainment,an endogenous time-keeping mechanism(biological clock)to respond to predictable changes in environmental conditions.Fish and fisheries are under pressure from both natural and anthropogenic perturbation,which in many cases alters the intensity and spectral composition of the light environment on which they depend for their survival.This review examines the effects of a changing light environment and turbidity on the health of fishes within a developmental and ecological context.Understanding the sensory environment of fishes is vital to predicting their responses and,ultimately,their resilience to environmental change and the potential for maintaining sustainable levels of biodiversity.展开更多
To reveal the nature of locusts’visual reaction when stimulated by light,and to clarify the regulation characteristics of locusts’phototactic visual physiology and obtain good spectral light features for locusts’ph...To reveal the nature of locusts’visual reaction when stimulated by light,and to clarify the regulation characteristics of locusts’phototactic visual physiology and obtain good spectral light features for locusts’phototactic action,this study investigated locusts’visual spectrum response by characterizing their photoreceptive reaction to LED light using an AvaSpec fiber-optic spectrometer system.Locusts’phototactic response to spectral light was compared using this system.The results showed that locusts’visual reaction characterization presents a photo-induced vision spectrum effect and that by offsetting the main wavelength of light and the spectral peak intensity,a time-varying bio-regulation effect emerges.In addition,locusts’visual regulation ability to UV light is higher than that to violet light,whereas their reaction intensity is lower than to violet light,and the visual bio-regulatory force of locusts’visual system absorbing orange light to react sensitively becomes gradually higher than when absorbing green light as time goes on.Moreover,corresponding to nominal illumination with the same radiant energy and a visual spectrum response stimulated by UV,violet,orange,and green light,it appears that the visual spectrum window is symmetrical around 382 nm,400 nm,602 nm,and 530 nm,respectively,with no significant difference between spectral amplitudes and having a time-varying incremental characteristic with amplitude peak width.This indicates that the stimulus intensity of UV,violet,orange,and green light exceeds locusts’visual tolerance,causing them to generate regulation inactivation as a visual physiological reaction,whereas the visual window response effect stimulated by UV light presents an illumination timeliness effect.Simultaneously,time-varying characteristics of locusts’bio-behavior intensity show that light intensity can make up for locusts’visual sensitivity differences at various spectral wavelengths.Presented with differential response time,photosensitive behavior intensity,and induction effect induced by orange light,time is superior for orange light,the stimulation effect caused by violet light is the strongest,and the phototactic synergy effect caused by UV light is the best.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD2401901)the Fundamental Research Funds for Zhejiang Provincial Universities and Research Institutes(2024J004)。
文摘The large yellow croaker(Larimichthys crocea)is a flagship marine fish in China given its extreme commercial value and golden-yellow coloration.However,the genetic mechanisms underlying golden-yellow coloration remain unclear.Here,we construct a telomere-to-telomere gap-free genome assembly(T2TLarcro_1.0)spanning 716.87 Mb,with a contig N50 of 31.75 Mb.Compared to the current reference genome(L_crocea_2.0),T2T-Larcro_1.0 incorporates 112.70 Mb of previously unassembled regions and 2368 newly anchored genes.This assembly facilitates comparative genomics analyses in sciaenids by identifying several candidate genes(e.g.,OPNVA,nNOS,RDH13)potentially involved in evolution of golden-yellow coloration.Transcriptomic analyses further confirm expression of OPNVA-encoded vertebrate ancient opsin(VA opsin)in skin tissues of the large yellow croaker,suggesting its role as an extraretinal photoreceptor regulating localized golden-yellow coloration.Integrating genomics and transcriptomics results,we uncover the triggering effect of VA opsin linking skin and neural photoreception to physiological regulation of body color change(golden-yellow to silvery-white)in L.crocea.Collectively,our findings provide molecular evidence that elucidate the underlying evolutionary mechanism of goldenyellow coloration in L.crocea.This high-quality genome assembly also serves as an improved resource for biological evolution,genetic improvement,and selective breeding of L.crocea.
文摘Vision and photoentrainment in fishes are vital for feeding,avoiding predation,spatial orientation,navigation,social communication and the synchronization of many homeostatic functions such as activity patterns and sleep.The camera-like(image-forming)eyes of fishes are optimized to provide a clear view of their preferred ecological niche,while non-visual photoreceptors provide irradiance detection that mediates circadian photoentrainment,an endogenous time-keeping mechanism(biological clock)to respond to predictable changes in environmental conditions.Fish and fisheries are under pressure from both natural and anthropogenic perturbation,which in many cases alters the intensity and spectral composition of the light environment on which they depend for their survival.This review examines the effects of a changing light environment and turbidity on the health of fishes within a developmental and ecological context.Understanding the sensory environment of fishes is vital to predicting their responses and,ultimately,their resilience to environmental change and the potential for maintaining sustainable levels of biodiversity.
基金The authors acknowledge that this work was financially supported by the Scientific and technological project in Henan Province(Grant No.212102110139)the China Agricultural Research System(Grant No.CARS-03)the Henan Engineering Research Center of Biological Pesticide&Fertilizer Development and Synergistic Application.
文摘To reveal the nature of locusts’visual reaction when stimulated by light,and to clarify the regulation characteristics of locusts’phototactic visual physiology and obtain good spectral light features for locusts’phototactic action,this study investigated locusts’visual spectrum response by characterizing their photoreceptive reaction to LED light using an AvaSpec fiber-optic spectrometer system.Locusts’phototactic response to spectral light was compared using this system.The results showed that locusts’visual reaction characterization presents a photo-induced vision spectrum effect and that by offsetting the main wavelength of light and the spectral peak intensity,a time-varying bio-regulation effect emerges.In addition,locusts’visual regulation ability to UV light is higher than that to violet light,whereas their reaction intensity is lower than to violet light,and the visual bio-regulatory force of locusts’visual system absorbing orange light to react sensitively becomes gradually higher than when absorbing green light as time goes on.Moreover,corresponding to nominal illumination with the same radiant energy and a visual spectrum response stimulated by UV,violet,orange,and green light,it appears that the visual spectrum window is symmetrical around 382 nm,400 nm,602 nm,and 530 nm,respectively,with no significant difference between spectral amplitudes and having a time-varying incremental characteristic with amplitude peak width.This indicates that the stimulus intensity of UV,violet,orange,and green light exceeds locusts’visual tolerance,causing them to generate regulation inactivation as a visual physiological reaction,whereas the visual window response effect stimulated by UV light presents an illumination timeliness effect.Simultaneously,time-varying characteristics of locusts’bio-behavior intensity show that light intensity can make up for locusts’visual sensitivity differences at various spectral wavelengths.Presented with differential response time,photosensitive behavior intensity,and induction effect induced by orange light,time is superior for orange light,the stimulation effect caused by violet light is the strongest,and the phototactic synergy effect caused by UV light is the best.
