OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necro...OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necrosis factor （TNF）-a, and interleu- kin （IL）-4, eosinophil counts, as well as expression of matrix metalloproteinase （MMP）-9, tissue inhibi- tor of metalloproteinase （TIMP）-I in the lung tissues of asthmatic rats. METHODS： Sixty clean-grade Sprague-Dawley rats were divided randomly into six groups： normal control （NC）, asthma model, Guilong Kechuanning （GK） group, as well as high, intermediate-, and lowdose XP groups. Rats were sensitized with ovalbu- min （OVA） to trigger asthma. Serum concentrations of ECP, TNF-a and IL-4, eosinophil counts, as well as expression of MMP-9 and TIMP-1 in lung tissues were evaluated using an immunofluorescence method, mRNA expression of MMP-9 and TIMP-1 was determined using real-time quantitative poly- merase chain reaction. RESULTS： Compared with the asthma-model group, serum concentrations of ECP, TNF-a, and IL-4, and eosinophil counts decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Protein expression of MMP-9 and TIMP-1 decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Transcription of MMP-9 and TIMP-1 mRNA and the ratio of expression of MMP-9：TIMP-1 in lung tissue were significantly lower （P 〈 0.01）. CONCLUSION： XP can reduce TNF-a secretion, sup- press the infiltration/activation of eosinophils, re- duce serum concentrations of ECP and IL-4, reduce the protein and mRNA expression of MMP-9 and TIMP-1 in lung tissue, and regulate the balance between expression of MMP-9 and TIMP-1. In these ways, XP alleviated the inflammation and remodel- ing of the airways.展开更多
Sense and Sensibility,one of Jane Austen's masterpieces,has undergone a long time study and the two sisters in the book—Elinor and Marianne have been researched from various kinds of perspectives except"mela...Sense and Sensibility,one of Jane Austen's masterpieces,has undergone a long time study and the two sisters in the book—Elinor and Marianne have been researched from various kinds of perspectives except"melancholy philosophy".This paper aims to analyze the two sisters in Sense and Sensibility under the guidance of"melancholy philosophy".展开更多
An extended state observer(ESO)-based loop filter is designed for the phase-locked loop(PLL)involved in a disturbed grid-connected converter(GeC).This ESO-based design enhances the performances and robustness of the P...An extended state observer(ESO)-based loop filter is designed for the phase-locked loop(PLL)involved in a disturbed grid-connected converter(GeC).This ESO-based design enhances the performances and robustness of the PLL,and,therefore,improves control performances of the disturbed GeCs.Besides,the ESO-based LF can be applied to PLLs with extra filters for abnormal grid conditions.The unbalanced grid is particularly taken into account for the performance analysis.A tuning approach based on the well-designed PI controller is discussed,which results in a fair comparison with conventional PI-type PLLs.The frequency domain properies are quantitatively analysed with respeet to the control stability and the noises rejection.The frequency domain analysis and simulation results suggesti that the performances of the generated ESO-based controllers are comparable to those of the PI control at low frequency,while have better ability to atenuate high-frequency measurement noises.The phase margin decreases slightly,but remains acceptable.Finally,experimental tests are conducted with a hybrid power hardwarein-the-loop benchmark,in which balanced/unbalanced cases are both explored.The obtained results prove the effectiveness of ESO based PLLs when applied to the disturbed GeC.展开更多
[Objective] This study was carried out to explore the effect of poly-β-hydroxybutyrate（PHB） on the performance of the nonspecific immune system in Fenneropenaeus chinensis. [Method] F. chinensis individuals were as...[Objective] This study was carried out to explore the effect of poly-β-hydroxybutyrate（PHB） on the performance of the nonspecific immune system in Fenneropenaeus chinensis. [Method] F. chinensis individuals were assigned into six groups, and each group was fed with a diet containing 0（Control）, 0.5%（Group E0.5）, 1.0%（Group E1.0）, 2.5%（Group E2.5）, 5.0%（Group E5.0） or 10.0% PHB（Group E10.0）. The mortality rate and relative percent of survival（RPS） of each group were calculated after 6 weeks. Meanwhile, the total antioxidant capacity（T-AOC）, the activity of acid phosphatase（ACP）, peroxidase（POD） and catalase（CAT）, and the content of malondialdehyde（MDA） in hepatopancreas and serum were measured,and their correlation with PHB concentration was analyzed. [Result] The RPS in PHB treated shrimps increased initially and decreased subsequently with increasing PHB concentration. RPS of Group E1.0 was the highest, showing significant difference from that of other groups（ P 〈0.05）.With the increase in PHB concentration, the activity of immunity related enzymes changed in a similar pattern with RPS： increasing at first and decreasing subsequently. In addition, the activity of the enzymes was elevated in the 2nd and 3rd weeks of PHB administration. Among them, T-AOC in serum of groups E1.0 and E2.5, T-AOC in hepatopancreas of Group E1.0, ACP activity in serum of groups E1.0 and E2.5, ACP activity in hepatopancreas of Group E1.0, CAT activity in groups E0.5, E1.0 and E2.5, CAT activity in hepatopancreas of groups E0.5, E1.0 and E10.0, POD activity in both serum and hepatopancreas of groups E0.5, E1.0 and E2.5, SOD activity in both serum and hepatopancreas of Group E1.0, MDA content in serum of Group E1.0 and MDA content in hepatopancreas of groups E0.5 and E1.0 showed significant difference from those of other groups（P〈0.05）. [Conclusion] PHB can improve the immunity of F. chinensis, 1.0% in feed has the best effect, and the total enzyme activity reaches the highest level in the 2nd and 3rd weeks of PHB administration.展开更多
2006年8月3日，托尼·班奈特度过他80岁的生日，为庆祝这个人生的里程碑，他决定与当代艺人携手重新用歌声记住生涯中每一个感动的时刻，推出跨界合唱大碟《Tony Bennett Duets An American Classic》，专辑由制作过辛纳屈合唱大碟...2006年8月3日，托尼·班奈特度过他80岁的生日，为庆祝这个人生的里程碑，他决定与当代艺人携手重新用歌声记住生涯中每一个感动的时刻，推出跨界合唱大碟《Tony Bennett Duets An American Classic》，专辑由制作过辛纳屈合唱大碟《Duets》与洛德史都华《American Songbook》系列专辑的格莱美奖金牌制作名家Phil Ra—mone操刀制作，展开更多
全球领先的PA66树脂和化学中间体生产商奥升德高性能材料有限公司日前宣布任命Phil Mc Divitt担任聚酰胺业务总裁。作为全球聚酰胺业务的负责人,Phil Mc Divitt将在美国休斯敦的奥升德总部任职,领导聚酰胺业务的三个生产基地和1800余名...全球领先的PA66树脂和化学中间体生产商奥升德高性能材料有限公司日前宣布任命Phil Mc Divitt担任聚酰胺业务总裁。作为全球聚酰胺业务的负责人,Phil Mc Divitt将在美国休斯敦的奥升德总部任职,领导聚酰胺业务的三个生产基地和1800余名员工。同时他也将成为奥升德管理团队的一员,向公司首席执行官Fred Poses汇报。展开更多
This paper presents the power hardware in the loop(PHIL)validation of a feed forward DC voltage control scheme for the fault ride through(FRT)of voltage source converter(VSC)high voltage DC(HVDC)connected offshore win...This paper presents the power hardware in the loop(PHIL)validation of a feed forward DC voltage control scheme for the fault ride through(FRT)of voltage source converter(VSC)high voltage DC(HVDC)connected offshore wind power plants(WPPs).In the proposed FRT scheme,the WPP collector network AC voltage is actively controlled by considering both the DC voltage error and the AC current from the WPP AC collector system which ensures fast and robust FRT of the VSC HVDC connected offshore WPPs.The PHIL tests were carried out in order to verify the efficacy of the proposed feed forward DC voltage control scheme for enhancing the FRT capability of the VSC HVDC connected WPPs.The PHIL test results have demonstrated the proper control coordination between the offshore WPP and the WPP side VSC and the efficient FRT of the VSC HVDC connected WPPs.展开更多
基金Supported by Natural Science Foundation of China:Intervention Mechanism of the Method of Tonifying Qi And Promoting Blood Circulation and Resolving Phlem on Airway Remodeling after COPD(No.81473675)Based on p38MAP Signal Pathway Yiqi Huoxue Huatan Method was Explored the Mechanism Study of Bronchial Asthma Airway Remodeling(No.81774301)Excellent Young Talent Fund Project of Colleges and Universities in Anhui Province:Study on the Mechanism of Airway Remodeling of Asthma Model In Rats With Xiaochuanping Powder(No.2011SQRL093)
文摘OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necrosis factor （TNF）-a, and interleu- kin （IL）-4, eosinophil counts, as well as expression of matrix metalloproteinase （MMP）-9, tissue inhibi- tor of metalloproteinase （TIMP）-I in the lung tissues of asthmatic rats. METHODS： Sixty clean-grade Sprague-Dawley rats were divided randomly into six groups： normal control （NC）, asthma model, Guilong Kechuanning （GK） group, as well as high, intermediate-, and lowdose XP groups. Rats were sensitized with ovalbu- min （OVA） to trigger asthma. Serum concentrations of ECP, TNF-a and IL-4, eosinophil counts, as well as expression of MMP-9 and TIMP-1 in lung tissues were evaluated using an immunofluorescence method, mRNA expression of MMP-9 and TIMP-1 was determined using real-time quantitative poly- merase chain reaction. RESULTS： Compared with the asthma-model group, serum concentrations of ECP, TNF-a, and IL-4, and eosinophil counts decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Protein expression of MMP-9 and TIMP-1 decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Transcription of MMP-9 and TIMP-1 mRNA and the ratio of expression of MMP-9：TIMP-1 in lung tissue were significantly lower （P 〈 0.01）. CONCLUSION： XP can reduce TNF-a secretion, sup- press the infiltration/activation of eosinophils, re- duce serum concentrations of ECP and IL-4, reduce the protein and mRNA expression of MMP-9 and TIMP-1 in lung tissue, and regulate the balance between expression of MMP-9 and TIMP-1. In these ways, XP alleviated the inflammation and remodel- ing of the airways.
文摘目的:构建人血管抑素K1-3的重组酵母胞内表达质粒PH IL-D2/K1-3,获得重组目的蛋白。方法:从胎儿肝脏组织用RT-PCR方法扩增人血管抑素K1-3目的基因,克隆至酵母胞内表达质粒PH IL-D2中,线性化后转化到毕赤酵母GS115胞内,经PCR和Sourthern杂交筛选出阳性转化子,并用甲醇诱导表达。表达产物进行SDS-PAGE(12%)和W estern b lot检测,经赖氨酸亲和层析柱纯化后,用Lowry法测定蛋白含量,并进行生物活性测定。结果:SDS-PAGE和W estern b lot结果显示,表达蛋白质的相对分子量为30kd左右,低糖基化。Lowry法测定蛋白表达量为6.8mg/L。活性测定实验表明重组血管抑素能特异抑制人血管内皮细胞的增殖。结论:在毕赤酵母GS115胞内成功表达了人血管抑素K1-3重组蛋白。
文摘Sense and Sensibility,one of Jane Austen's masterpieces,has undergone a long time study and the two sisters in the book—Elinor and Marianne have been researched from various kinds of perspectives except"melancholy philosophy".This paper aims to analyze the two sisters in Sense and Sensibility under the guidance of"melancholy philosophy".
基金This paper was supported by G2elab,Grenoble INP,University Grenoble Alpes,France and School of Engineering,HES-sO,Valais,Switzerlandfunding provided by Haute Ecole Specialisee de Suisse occidentale(HES-SO)
文摘An extended state observer(ESO)-based loop filter is designed for the phase-locked loop(PLL)involved in a disturbed grid-connected converter(GeC).This ESO-based design enhances the performances and robustness of the PLL,and,therefore,improves control performances of the disturbed GeCs.Besides,the ESO-based LF can be applied to PLLs with extra filters for abnormal grid conditions.The unbalanced grid is particularly taken into account for the performance analysis.A tuning approach based on the well-designed PI controller is discussed,which results in a fair comparison with conventional PI-type PLLs.The frequency domain properies are quantitatively analysed with respeet to the control stability and the noises rejection.The frequency domain analysis and simulation results suggesti that the performances of the generated ESO-based controllers are comparable to those of the PI control at low frequency,while have better ability to atenuate high-frequency measurement noises.The phase margin decreases slightly,but remains acceptable.Finally,experimental tests are conducted with a hybrid power hardwarein-the-loop benchmark,in which balanced/unbalanced cases are both explored.The obtained results prove the effectiveness of ESO based PLLs when applied to the disturbed GeC.
基金Supported by Central Public-interest Scientific Institution Basal Research Fund,Yellow Sea Fisheries Research Institute,CAFS(20603022017001)National Natural Science Foundation of China(41676148)+1 种基金Taishan Scholar Program for Seed IndustryChina Agriculture Research System(CARS-48)
文摘[Objective] This study was carried out to explore the effect of poly-β-hydroxybutyrate（PHB） on the performance of the nonspecific immune system in Fenneropenaeus chinensis. [Method] F. chinensis individuals were assigned into six groups, and each group was fed with a diet containing 0（Control）, 0.5%（Group E0.5）, 1.0%（Group E1.0）, 2.5%（Group E2.5）, 5.0%（Group E5.0） or 10.0% PHB（Group E10.0）. The mortality rate and relative percent of survival（RPS） of each group were calculated after 6 weeks. Meanwhile, the total antioxidant capacity（T-AOC）, the activity of acid phosphatase（ACP）, peroxidase（POD） and catalase（CAT）, and the content of malondialdehyde（MDA） in hepatopancreas and serum were measured,and their correlation with PHB concentration was analyzed. [Result] The RPS in PHB treated shrimps increased initially and decreased subsequently with increasing PHB concentration. RPS of Group E1.0 was the highest, showing significant difference from that of other groups（ P 〈0.05）.With the increase in PHB concentration, the activity of immunity related enzymes changed in a similar pattern with RPS： increasing at first and decreasing subsequently. In addition, the activity of the enzymes was elevated in the 2nd and 3rd weeks of PHB administration. Among them, T-AOC in serum of groups E1.0 and E2.5, T-AOC in hepatopancreas of Group E1.0, ACP activity in serum of groups E1.0 and E2.5, ACP activity in hepatopancreas of Group E1.0, CAT activity in groups E0.5, E1.0 and E2.5, CAT activity in hepatopancreas of groups E0.5, E1.0 and E10.0, POD activity in both serum and hepatopancreas of groups E0.5, E1.0 and E2.5, SOD activity in both serum and hepatopancreas of Group E1.0, MDA content in serum of Group E1.0 and MDA content in hepatopancreas of groups E0.5 and E1.0 showed significant difference from those of other groups（P〈0.05）. [Conclusion] PHB can improve the immunity of F. chinensis, 1.0% in feed has the best effect, and the total enzyme activity reaches the highest level in the 2nd and 3rd weeks of PHB administration.
文摘2006年8月3日，托尼·班奈特度过他80岁的生日，为庆祝这个人生的里程碑，他决定与当代艺人携手重新用歌声记住生涯中每一个感动的时刻，推出跨界合唱大碟《Tony Bennett Duets An American Classic》，专辑由制作过辛纳屈合唱大碟《Duets》与洛德史都华《American Songbook》系列专辑的格莱美奖金牌制作名家Phil Ra—mone操刀制作，
文摘全球领先的PA66树脂和化学中间体生产商奥升德高性能材料有限公司日前宣布任命Phil Mc Divitt担任聚酰胺业务总裁。作为全球聚酰胺业务的负责人,Phil Mc Divitt将在美国休斯敦的奥升德总部任职,领导聚酰胺业务的三个生产基地和1800余名员工。同时他也将成为奥升德管理团队的一员,向公司首席执行官Fred Poses汇报。
文摘This paper presents the power hardware in the loop(PHIL)validation of a feed forward DC voltage control scheme for the fault ride through(FRT)of voltage source converter(VSC)high voltage DC(HVDC)connected offshore wind power plants(WPPs).In the proposed FRT scheme,the WPP collector network AC voltage is actively controlled by considering both the DC voltage error and the AC current from the WPP AC collector system which ensures fast and robust FRT of the VSC HVDC connected offshore WPPs.The PHIL tests were carried out in order to verify the efficacy of the proposed feed forward DC voltage control scheme for enhancing the FRT capability of the VSC HVDC connected WPPs.The PHIL test results have demonstrated the proper control coordination between the offshore WPP and the WPP side VSC and the efficient FRT of the VSC HVDC connected WPPs.
