BACKGROUND Many conditions may affect left ventricular(LV)phenotypes which have been classified according to LV mass and geometry.There is limited data on the prognostic value of LV phenotypes classified by cardiac ma...BACKGROUND Many conditions may affect left ventricular(LV)phenotypes which have been classified according to LV mass and geometry.There is limited data on the prognostic value of LV phenotypes classified by cardiac magnetic resonance(CMR).This study aimed to determine the prognostic value of LV phenotypes in elderly and non-elderly patients with known or suspected coronary artery disease.METHODS This is a retrospective cohort study among patients who underwent stress or viability CMR.LV phenotypes were classified according to the LV mass index,the LV end-diastolic volume index and the LV mass/volume ratio,into normal,concentric remodeling,concentric hypertrophy,and eccentric hypertrophy.The primary outcome was a composite of death or heart failure.RESULTS A total of 3289 patients was studied.The average age was 68.0±12.7 years,52.2%of patients were women.Elderly were defined as age≥65 years accounting for 63.9%of the cohort.LV phenotypes were normal,concentric remodeling,concentric hypertrophy,and eccentric hypertrophy at 74.5%,5.8%,9.2%,and 10.5%,respectively.The median duration of follow-up was 41.4 months.The composite outcome of death or heart failure occurred in 7.3%of patients.The prognostic impact of LV phenotypes was more pronounced in the elderly,with eccentric hypertrophy showing the worst prognosis,followed by concentric hypertrophy and concentric remodeling with the adjusted hazard ratio(95%CI)of 2.37(1.72–3.25),1.53(1.12–2.08),and 1.14(0.76–1.71),respectively,compared to normal phenotype.Patients with eccentric hypertrophy also demonstrated abnormal global longitudinal LV strain,left atrial strain,and extracellular volume fraction.CONCLUSIONS LV phenotypes by CMR independently predict adverse clinical outcomes in elderly patients with known or suspected coronary artery disease.In non-elderly patients,the prognostic value of LV phenotypes was less evident.Assessment of LV phenotypes may be useful for risk stratification.展开更多
Nondestructive measurement technology of phenotype can provide substantial phenotypic data support for applications such as seedling breeding,management,and quality testing.The current method of measuring seedling phe...Nondestructive measurement technology of phenotype can provide substantial phenotypic data support for applications such as seedling breeding,management,and quality testing.The current method of measuring seedling phenotypes mainly relies on manual measurement which is inefficient,subjective and destroys samples.Therefore,the paper proposes a nondestructive measurement method for the canopy phenotype of the watermelon plug seedlings based on deep learning.The Azure Kinect was used to shoot canopy color images,depth images,and RGB-D images of the watermelon plug seedlings.The Mask-RCNN network was used to classify,segment,and count the canopy leaves of the watermelon plug seedlings.To reduce the error of leaf area measurement caused by mutual occlusion of leaves,the leaves were repaired by CycleGAN,and the depth images were restored by image processing.Then,the Delaunay triangulation was adopted to measure the leaf area in the leaf point cloud.The YOLOX target detection network was used to identify the growing point position of each seedling on the plug tray.Then the depth differences between the growing point and the upper surface of the plug tray were calculated to obtain plant height.The experiment results show that the nondestructive measurement algorithm proposed in this paper achieves good measurement performance for the watermelon plug seedlings from the 1 true-leaf to 3 true-leaf stages.The average relative error of measurement is 2.33%for the number of true leaves,4.59%for the number of cotyledons,8.37%for the leaf area,and 3.27%for the plant height.The experiment results demonstrate that the proposed algorithm in this paper provides an effective solution for the nondestructive measurement of the canopy phenotype of the plug seedlings.展开更多
Aging is a universal biological process characterized by the progressive decline in cellular and tissue function,representing the main risk factor for the development of most chronic human diseases.At the cellular lev...Aging is a universal biological process characterized by the progressive decline in cellular and tissue function,representing the main risk factor for the development of most chronic human diseases.At the cellular level,one hallmark of aging is the accumulation of senescent cells—non-dividing yet metabolically active cells that adopt a unique phenotype,including the senescence-associated secretory phenotype(SASP)(Wang et al.,2024).展开更多
Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed t...Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed the cell biology characters of ONFH osteonecrosis area bone tissue in single cell scale and thus identified a novel ONFH treatment approach based on M2 macrophages-derived exosomes(M2-Exos).We further show that M2-Exos are highly effective in the treatment of ONFH by modulating the phenotypes communication between neutrophil and endothelium including neutrophil extracellular traps formation and endothelial phenotype transition.Additionally,we identified that M2-Exos’therapeutic effect is attributed to the high content of miR-93-5p and constructed miR-93-5p overexpression model in vitro and in vivo based on lentivirus and adenoassociated virus respectively.Then we found miR-93-5p can not only reduce neutrophil extracellular traps formation but also improve angiogenic ability of endothelial cells.These results provided a new theoretical basis for the clinical application of ONFH therapeutic exosomes.展开更多
The Rh blood group system,especially the D antigen,is crucial in transfusion medicine and obstetrics.Weak D phenotypes,caused by mutations in the Rhesus D antigen(RhD)blood group(RHD)gene,result in reduced antigen exp...The Rh blood group system,especially the D antigen,is crucial in transfusion medicine and obstetrics.Weak D phenotypes,caused by mutations in the Rhesus D antigen(RhD)blood group(RHD)gene,result in reduced antigen expression,posing challenges in serological testing and clinical management.Variability in detection methods leads to inconsistent results,making accurate classification difficult.Molecular techniques like polymerase chain reaction and DNA sequencing have significantly improved the identification of weak D variants,offering more reliable transfusion strategies and reducing the risk of alloimmunization.However,challenges such as lack of standardized protocols,cost constraints,and population-specific variations remain.In obstetrics,proper management of pregnant women with weak D is essential to prevent hemolytic disease of the fetus and newborn.Non-invasive prenatal testing using cell-free fetal DNA shows promise in predicting RhD incompatibility and minimizing unnecessary Rh immune globulin administration.Future advancements in highthroughput genotyping and discovery of novel RHD alleles could enhance RhD testing accuracy and efficiency.Standardizing RHD genotyping and adopting genotype-based management strategies for Rh immune globulin therapy and red blood cell transfusions will improve patient safety and clinical outcomes.This review examines the molecular basis,challenges,and future prospects in weak D phenotype management.展开更多
The mussel is one of the main cultivated species in the world.A significant challenge faced by suspension-cultured mussels is the high incidence of mussel fall-off from cultivation ropes,adversely impacting harvest yi...The mussel is one of the main cultivated species in the world.A significant challenge faced by suspension-cultured mussels is the high incidence of mussel fall-off from cultivation ropes,adversely impacting harvest yields,which have been documented at commercial mussel farms in the United Kingdom,the United States of America,Canada,Spain,New Zealand and China.Byssus is an important attachment structure for marine mussels,and weakness in byssal thread attachment is a major factor leading to mussel detachment from ropes.To investigate the relationship between genetic variability and byssal thread phenotypic characteristics in the hard-shelled mussel(Mytilus coruscus),we collected three wild populations of M.coruscus from different latitudes in the East China Sea,including the Shengsi(SS),Jiaojiang(JJ),and Fuding(FD)populations.The genetic diversity and structure of these populations were investigated using 10 microsatellite loci.The mean observed heterozygosity(Ho)in the SS population was 0.44,higher than the mean Ho values of the JJ(0.40)and FD(0.39)populations.The mean inbreeding coefficients(F_(is))in the SS population was 0.20,lower than the mean F_(is)values of the JJ(0.33)and FD populations(0.40).These results revealed that the SS population exhibited higher genetic diversity compared to the other two populations.The different numbers of private alleles(P_(a))in the three populations,ranging from 10 to 17,suggest that these populations have experienced selective pressures from various environments.Moreover,genetic differentiation was observed in the genetic distance between the SS population and the other two populations.We also examined the phenotypic characteristics of their byssal threads.There were significant differences in byssus attachment strength among the three populations,with the SS population located at the highest latitude secreting more byssal threads and exhibiting greater byssal breaking force and plaque adhesion strength,while the Fuding(FD)population located at the lowest latitude had the weakest byssal attachment.The observed differentiation in private alleles and byssus phenotypes might suggest that the three wild populations have experienced different environmental selective pressures.This study provides insight for future genetic enhancement programs aimed at improving byssus attachment in M.coruscus.展开更多
Hypertrophic cardiomyopathy(HCM)is the most common genetic cardiovascular disease,mostly inherited in an autosomal dominant manner.It is a global heart disease with complex clinical phenotypes and gene expression.The ...Hypertrophic cardiomyopathy(HCM)is the most common genetic cardiovascular disease,mostly inherited in an autosomal dominant manner.It is a global heart disease with complex clinical phenotypes and gene expression.The prevalence rate in the population is 1:500-1:200.This article mainly introduces the diagnostic criteria,pathological manifestations,and genetic basis of HCM,which is the leading cause of sudden death in adolescents and athletes due to exercise,with 60%-70%showing familial clustering.It also discusses the latest progress in the relationship between different genotypes and clinical phenotypes of HCM pa-tients.展开更多
Thrombin blockers have been shown to be effective for various pathological conditions,but their use is limited due to the potential for serious bleeding adverse effects.This study introduced a novel bioactive peptide(...Thrombin blockers have been shown to be effective for various pathological conditions,but their use is limited due to the potential for serious bleeding adverse effects.This study introduced a novel bioactive peptide(P-2-CG) from oyster,that mitigated thrombin-mediated barrier dysfunction and prothrombotic phenotypes in human pulmonary microvascular endothelial cells(HPMECs).P-2-CG significantly attenuated the increase in endothelial monolayer permeability induced by thrombin through the possible attenuation of RhoA activation and it promoted barrier recovery by enhancing endothelial cell adhesion.Additionally,P-2-CG was found to decrease the pro-thrombotic phenotype induced by thrombin in HPMEC by reducing the extrinsic trigger tissue factor mRNA expression,which resulted in prolonged plasma clotting time,decreased Factor Xa activation,and reduced thrombin generation.Moreover,P-2-CG inhibited thrombosis efficiently by blocking intercellular adhesion molecule 1 and vascular cell adhesion protein 1 expression via tyrosine phosphorylation of nuclear factor-κB p65.P-2-CG inhibits thrombin mediated inflammation and provides a potential therapeutic option for treating endothelial dysfunction and thrombosis.展开更多
Highlights●CRISPR/Cas9 RNP complex-based strategy demonstrates robustness and accuracy in generating gene-edited sheep.●Sheep horn development remains unaffected by partial RXFP2 knockout.●Partial RXFP2 knockout re...Highlights●CRISPR/Cas9 RNP complex-based strategy demonstrates robustness and accuracy in generating gene-edited sheep.●Sheep horn development remains unaffected by partial RXFP2 knockout.●Partial RXFP2 knockout results in unilateral cryptorchidism in sheep.展开更多
Objectives:By investigating the distinct speech and voice phenotype among TCM constitution for adults,this study aims at providing a convenient and objective methodological reference for judging TCM constitution.Metho...Objectives:By investigating the distinct speech and voice phenotype among TCM constitution for adults,this study aims at providing a convenient and objective methodological reference for judging TCM constitution.Methods:Acoustic analysis and TCM constitution assessment were performed for all 620 participants using Praat software and the CCMQ,respectively.Results:For formant features,the speech duration of special constitution participants was shorter than that of neutral,phlegm-dampness,dampness-heat,Yin-deficiency,or Yang-deficiency participants when pronuncing the vowels/a/,/i/,and/u/.Compare to Yang-deficiency,Qi-deficiency participants had a shorter speech duration when pronucing/i/.For/u/,blood-stasis participants exhibited a lower F1 value than neutral participants.For vocal features,special constitution participants showed higher local jitter than neutral,dampness-heat,and Yang-deficiency participants(for/a/,/i/,and/u/).Higher absolute local jitter than neutral or dampness-heat participants.Compared with neutral or Yang-deficiency participants,special participants owned a higher local shimmer(dB).Special participants had a lower harmonicity autocorrelation than neutral,dampness-heat,or Yang-deficiency participants.Conclusions:Formant features may effectively differentiate special constitution from neutral,phlegm-dampness,dampness-heat,Yin-deficiency,or Yang-deficiency constitutions based on vowel duration measurements(/a/,/i/,/u/).For the vowel/u/,F1 values may help distinguish blood-stasis from neutral constitution.Vocal features appear particularly useful for distinguishing special constitution from neutral,dampness-heat,or Yang-deficiency constitution,with local jitter and harmonicity autocorrelation showing significant discriminatory power.展开更多
面向作物表型组大数据获取解析、作物种质资源表型鉴定等亟需高效率、智能化和低成本技术、装备及系统的问题,在系统梳理分析国内外农作物高通量表型平台相关技术产品研究现状的基础上,通过组织多学科的协同技术攻关,突破了作物表型组...面向作物表型组大数据获取解析、作物种质资源表型鉴定等亟需高效率、智能化和低成本技术、装备及系统的问题,在系统梳理分析国内外农作物高通量表型平台相关技术产品研究现状的基础上,通过组织多学科的协同技术攻关,突破了作物表型组大数据高通量获取和智能化解析中的关键技术难题,设计了具有自主知识产权的轻小敏捷型多传感器阵列、通用化成像单元和适用于多生境的固定式、移动式高通量表型平台装备,以及配套算法和软件平台,构建了农作物表型组大数据工厂成套技术装备体系。该体系由大田和设施作物高通量自主作业表型平台、室内器官和显微表型平台、大田和设施环境自动化种植管控设备、作物模型系统、数字孪生智慧管控平台和大数据计算服务中心等构成,可实现多生境、自动化、高通量、高效率、高精度的多源作物表型-环境数据协同采集,涵盖农作物群体、个体、器官和显微多重尺度,能够重建农林作物的三维形态结构并精准解析株型、产品、品质、抗性等表型组指标,是发展数字育种和智慧栽培的新一代信息化基础设施。农作物表型组大数据工厂技术装备体系创新了作物表型组大数据的产生、处理和服务模式,可为作物表型组理论技术的发展、基于AI for Science的平台化科研和工厂化的作物种质资源表型鉴定等提供体系化的技术装备支撑。展开更多
文摘BACKGROUND Many conditions may affect left ventricular(LV)phenotypes which have been classified according to LV mass and geometry.There is limited data on the prognostic value of LV phenotypes classified by cardiac magnetic resonance(CMR).This study aimed to determine the prognostic value of LV phenotypes in elderly and non-elderly patients with known or suspected coronary artery disease.METHODS This is a retrospective cohort study among patients who underwent stress or viability CMR.LV phenotypes were classified according to the LV mass index,the LV end-diastolic volume index and the LV mass/volume ratio,into normal,concentric remodeling,concentric hypertrophy,and eccentric hypertrophy.The primary outcome was a composite of death or heart failure.RESULTS A total of 3289 patients was studied.The average age was 68.0±12.7 years,52.2%of patients were women.Elderly were defined as age≥65 years accounting for 63.9%of the cohort.LV phenotypes were normal,concentric remodeling,concentric hypertrophy,and eccentric hypertrophy at 74.5%,5.8%,9.2%,and 10.5%,respectively.The median duration of follow-up was 41.4 months.The composite outcome of death or heart failure occurred in 7.3%of patients.The prognostic impact of LV phenotypes was more pronounced in the elderly,with eccentric hypertrophy showing the worst prognosis,followed by concentric hypertrophy and concentric remodeling with the adjusted hazard ratio(95%CI)of 2.37(1.72–3.25),1.53(1.12–2.08),and 1.14(0.76–1.71),respectively,compared to normal phenotype.Patients with eccentric hypertrophy also demonstrated abnormal global longitudinal LV strain,left atrial strain,and extracellular volume fraction.CONCLUSIONS LV phenotypes by CMR independently predict adverse clinical outcomes in elderly patients with known or suspected coronary artery disease.In non-elderly patients,the prognostic value of LV phenotypes was less evident.Assessment of LV phenotypes may be useful for risk stratification.
基金funded by the National Key Research and Development Program of China(Grant No.2019YFD1001900)the HZAU-AGIS Cooperation Fund(Grant No.SZYJY2022006).
文摘Nondestructive measurement technology of phenotype can provide substantial phenotypic data support for applications such as seedling breeding,management,and quality testing.The current method of measuring seedling phenotypes mainly relies on manual measurement which is inefficient,subjective and destroys samples.Therefore,the paper proposes a nondestructive measurement method for the canopy phenotype of the watermelon plug seedlings based on deep learning.The Azure Kinect was used to shoot canopy color images,depth images,and RGB-D images of the watermelon plug seedlings.The Mask-RCNN network was used to classify,segment,and count the canopy leaves of the watermelon plug seedlings.To reduce the error of leaf area measurement caused by mutual occlusion of leaves,the leaves were repaired by CycleGAN,and the depth images were restored by image processing.Then,the Delaunay triangulation was adopted to measure the leaf area in the leaf point cloud.The YOLOX target detection network was used to identify the growing point position of each seedling on the plug tray.Then the depth differences between the growing point and the upper surface of the plug tray were calculated to obtain plant height.The experiment results show that the nondestructive measurement algorithm proposed in this paper achieves good measurement performance for the watermelon plug seedlings from the 1 true-leaf to 3 true-leaf stages.The average relative error of measurement is 2.33%for the number of true leaves,4.59%for the number of cotyledons,8.37%for the leaf area,and 3.27%for the plant height.The experiment results demonstrate that the proposed algorithm in this paper provides an effective solution for the nondestructive measurement of the canopy phenotype of the plug seedlings.
基金NIH NIA1RO1AG061879 and 5PO1AG066591(to LME)FONDAP Program 15150012,ECOS-A NID(ECOS230034)the US Army Medical Research Acquisition Activity(USAMRAA)AL2201415(to CH)。
文摘Aging is a universal biological process characterized by the progressive decline in cellular and tissue function,representing the main risk factor for the development of most chronic human diseases.At the cellular level,one hallmark of aging is the accumulation of senescent cells—non-dividing yet metabolically active cells that adopt a unique phenotype,including the senescence-associated secretory phenotype(SASP)(Wang et al.,2024).
基金the support of the National Natural Science Foundation of China (Grant No.82272503)Natural Science Foundation of Zhejiang Province (Grant No. LQN25H060006)
文摘Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed the cell biology characters of ONFH osteonecrosis area bone tissue in single cell scale and thus identified a novel ONFH treatment approach based on M2 macrophages-derived exosomes(M2-Exos).We further show that M2-Exos are highly effective in the treatment of ONFH by modulating the phenotypes communication between neutrophil and endothelium including neutrophil extracellular traps formation and endothelial phenotype transition.Additionally,we identified that M2-Exos’therapeutic effect is attributed to the high content of miR-93-5p and constructed miR-93-5p overexpression model in vitro and in vivo based on lentivirus and adenoassociated virus respectively.Then we found miR-93-5p can not only reduce neutrophil extracellular traps formation but also improve angiogenic ability of endothelial cells.These results provided a new theoretical basis for the clinical application of ONFH therapeutic exosomes.
文摘The Rh blood group system,especially the D antigen,is crucial in transfusion medicine and obstetrics.Weak D phenotypes,caused by mutations in the Rhesus D antigen(RhD)blood group(RHD)gene,result in reduced antigen expression,posing challenges in serological testing and clinical management.Variability in detection methods leads to inconsistent results,making accurate classification difficult.Molecular techniques like polymerase chain reaction and DNA sequencing have significantly improved the identification of weak D variants,offering more reliable transfusion strategies and reducing the risk of alloimmunization.However,challenges such as lack of standardized protocols,cost constraints,and population-specific variations remain.In obstetrics,proper management of pregnant women with weak D is essential to prevent hemolytic disease of the fetus and newborn.Non-invasive prenatal testing using cell-free fetal DNA shows promise in predicting RhD incompatibility and minimizing unnecessary Rh immune globulin administration.Future advancements in highthroughput genotyping and discovery of novel RHD alleles could enhance RhD testing accuracy and efficiency.Standardizing RHD genotyping and adopting genotype-based management strategies for Rh immune globulin therapy and red blood cell transfusions will improve patient safety and clinical outcomes.This review examines the molecular basis,challenges,and future prospects in weak D phenotype management.
文摘The mussel is one of the main cultivated species in the world.A significant challenge faced by suspension-cultured mussels is the high incidence of mussel fall-off from cultivation ropes,adversely impacting harvest yields,which have been documented at commercial mussel farms in the United Kingdom,the United States of America,Canada,Spain,New Zealand and China.Byssus is an important attachment structure for marine mussels,and weakness in byssal thread attachment is a major factor leading to mussel detachment from ropes.To investigate the relationship between genetic variability and byssal thread phenotypic characteristics in the hard-shelled mussel(Mytilus coruscus),we collected three wild populations of M.coruscus from different latitudes in the East China Sea,including the Shengsi(SS),Jiaojiang(JJ),and Fuding(FD)populations.The genetic diversity and structure of these populations were investigated using 10 microsatellite loci.The mean observed heterozygosity(Ho)in the SS population was 0.44,higher than the mean Ho values of the JJ(0.40)and FD(0.39)populations.The mean inbreeding coefficients(F_(is))in the SS population was 0.20,lower than the mean F_(is)values of the JJ(0.33)and FD populations(0.40).These results revealed that the SS population exhibited higher genetic diversity compared to the other two populations.The different numbers of private alleles(P_(a))in the three populations,ranging from 10 to 17,suggest that these populations have experienced selective pressures from various environments.Moreover,genetic differentiation was observed in the genetic distance between the SS population and the other two populations.We also examined the phenotypic characteristics of their byssal threads.There were significant differences in byssus attachment strength among the three populations,with the SS population located at the highest latitude secreting more byssal threads and exhibiting greater byssal breaking force and plaque adhesion strength,while the Fuding(FD)population located at the lowest latitude had the weakest byssal attachment.The observed differentiation in private alleles and byssus phenotypes might suggest that the three wild populations have experienced different environmental selective pressures.This study provides insight for future genetic enhancement programs aimed at improving byssus attachment in M.coruscus.
基金Supported by National Natural Science Foundation of China,No.82230065,No.82371974 and No.82272009.
文摘Hypertrophic cardiomyopathy(HCM)is the most common genetic cardiovascular disease,mostly inherited in an autosomal dominant manner.It is a global heart disease with complex clinical phenotypes and gene expression.The prevalence rate in the population is 1:500-1:200.This article mainly introduces the diagnostic criteria,pathological manifestations,and genetic basis of HCM,which is the leading cause of sudden death in adolescents and athletes due to exercise,with 60%-70%showing familial clustering.It also discusses the latest progress in the relationship between different genotypes and clinical phenotypes of HCM pa-tients.
基金supported by The National Natural Science Foundation of China (32130085 and 32202021)。
文摘Thrombin blockers have been shown to be effective for various pathological conditions,but their use is limited due to the potential for serious bleeding adverse effects.This study introduced a novel bioactive peptide(P-2-CG) from oyster,that mitigated thrombin-mediated barrier dysfunction and prothrombotic phenotypes in human pulmonary microvascular endothelial cells(HPMECs).P-2-CG significantly attenuated the increase in endothelial monolayer permeability induced by thrombin through the possible attenuation of RhoA activation and it promoted barrier recovery by enhancing endothelial cell adhesion.Additionally,P-2-CG was found to decrease the pro-thrombotic phenotype induced by thrombin in HPMEC by reducing the extrinsic trigger tissue factor mRNA expression,which resulted in prolonged plasma clotting time,decreased Factor Xa activation,and reduced thrombin generation.Moreover,P-2-CG inhibited thrombosis efficiently by blocking intercellular adhesion molecule 1 and vascular cell adhesion protein 1 expression via tyrosine phosphorylation of nuclear factor-κB p65.P-2-CG inhibits thrombin mediated inflammation and provides a potential therapeutic option for treating endothelial dysfunction and thrombosis.
基金supported by the National Key Research and Development Program of China(2022YFD1300200)the National Natural Science Foundation of China(32161143010,32202646,and 32272848)+2 种基金the China Agriculture Research System(CARS-39)the Key Special Project of Ningxia Science and Technology Department,China(2021BEF02024)the local grants,China(NXTS2021-001,2022GD-TSLD-46,NK2022010207,and NXTS2022-001)。
文摘Highlights●CRISPR/Cas9 RNP complex-based strategy demonstrates robustness and accuracy in generating gene-edited sheep.●Sheep horn development remains unaffected by partial RXFP2 knockout.●Partial RXFP2 knockout results in unilateral cryptorchidism in sheep.
基金supported by the National Natural Science Foundation of China(Nos.81730107 and 81973883)the National Science&Technology Basic Research Project(No.2015FY111700)the Shanghai Pudong New District New Area Project(No.PW2022A-78(WQZ)).
文摘Objectives:By investigating the distinct speech and voice phenotype among TCM constitution for adults,this study aims at providing a convenient and objective methodological reference for judging TCM constitution.Methods:Acoustic analysis and TCM constitution assessment were performed for all 620 participants using Praat software and the CCMQ,respectively.Results:For formant features,the speech duration of special constitution participants was shorter than that of neutral,phlegm-dampness,dampness-heat,Yin-deficiency,or Yang-deficiency participants when pronuncing the vowels/a/,/i/,and/u/.Compare to Yang-deficiency,Qi-deficiency participants had a shorter speech duration when pronucing/i/.For/u/,blood-stasis participants exhibited a lower F1 value than neutral participants.For vocal features,special constitution participants showed higher local jitter than neutral,dampness-heat,and Yang-deficiency participants(for/a/,/i/,and/u/).Higher absolute local jitter than neutral or dampness-heat participants.Compared with neutral or Yang-deficiency participants,special participants owned a higher local shimmer(dB).Special participants had a lower harmonicity autocorrelation than neutral,dampness-heat,or Yang-deficiency participants.Conclusions:Formant features may effectively differentiate special constitution from neutral,phlegm-dampness,dampness-heat,Yin-deficiency,or Yang-deficiency constitutions based on vowel duration measurements(/a/,/i/,/u/).For the vowel/u/,F1 values may help distinguish blood-stasis from neutral constitution.Vocal features appear particularly useful for distinguishing special constitution from neutral,dampness-heat,or Yang-deficiency constitution,with local jitter and harmonicity autocorrelation showing significant discriminatory power.
文摘面向作物表型组大数据获取解析、作物种质资源表型鉴定等亟需高效率、智能化和低成本技术、装备及系统的问题,在系统梳理分析国内外农作物高通量表型平台相关技术产品研究现状的基础上,通过组织多学科的协同技术攻关,突破了作物表型组大数据高通量获取和智能化解析中的关键技术难题,设计了具有自主知识产权的轻小敏捷型多传感器阵列、通用化成像单元和适用于多生境的固定式、移动式高通量表型平台装备,以及配套算法和软件平台,构建了农作物表型组大数据工厂成套技术装备体系。该体系由大田和设施作物高通量自主作业表型平台、室内器官和显微表型平台、大田和设施环境自动化种植管控设备、作物模型系统、数字孪生智慧管控平台和大数据计算服务中心等构成,可实现多生境、自动化、高通量、高效率、高精度的多源作物表型-环境数据协同采集,涵盖农作物群体、个体、器官和显微多重尺度,能够重建农林作物的三维形态结构并精准解析株型、产品、品质、抗性等表型组指标,是发展数字育种和智慧栽培的新一代信息化基础设施。农作物表型组大数据工厂技术装备体系创新了作物表型组大数据的产生、处理和服务模式,可为作物表型组理论技术的发展、基于AI for Science的平台化科研和工厂化的作物种质资源表型鉴定等提供体系化的技术装备支撑。
