Objectives:The chemical constituents of Poria cocos grown with different substrates vary significantly;thus,identifying and comparing their biomarkers are important.Materials and Methods:Herein,the chemical constituen...Objectives:The chemical constituents of Poria cocos grown with different substrates vary significantly;thus,identifying and comparing their biomarkers are important.Materials and Methods:Herein,the chemical constituents of Poria cocos obtained with five different substrates were assessed using gas chromatography–ion mobility spectrometry(GC-IMS),high-performance liquid chromatography and multivariate statistical analysis.Results:The content of moisture,ash,alcohol-soluble matter,and heavy metals,except for those of the miscellaneous wood Poria cocos,conform to the specifications defined in the Chinese Pharmacopoeia(Edition 2020),and the polysaccharide contents are all greater than 57%.Conclusions:Based on GC-IMS and the established fingerprints,87 compounds were detected,70 of which were identified in each group.Multivariate statistical analysis revealed seven compounds(two esters,three alcohols,and two aldehydes),which could be considered as potential marker compounds for discrimination.展开更多
Objective:To establish a progressive research strategy for“colonic components analysis-efficacy verification and mechanism exploration-gut microbiota”,screen pharmacodynamic substances,and investigate their mechanis...Objective:To establish a progressive research strategy for“colonic components analysis-efficacy verification and mechanism exploration-gut microbiota”,screen pharmacodynamic substances,and investigate their mechanism via gut microbiota.Methods:The pharmacodynamics of Gegen Qinlian decoction(GQD)were assessed using a mouse model of dextran sulfate sodium-induced ulcerative colitis(UC).Ultra-performance liquid chromatographyquadrupole-orbitrap mass spectrometer was used to identify the prototype and metabolic components of GQD in the colon during UC.To analyze the structure and function of characteristic genera of GQD and its active components,16S rRNA sequencing was performed.Results:We identified 67 prototypic and 14 metabolic components of GQD in the UC colon.The primary prototype components are flavonoids and alkaloids,including puerarin(PUE),baicalin(BAI),and berberine(BER).The metabolism was predominantly sulfonation.Efficacy verification showed that the main active components,puerarin,baicalin,and berberine,had good therapeutic effects on UC.The results of 16S rRNA gene sequencing showed that GQD improved UC by regulating the structure and function of the gut microbiota.The abundance of gut microbiota involved in the metabolism of the prototype componentswas influenced by the corresponding components.The function prediction results showed that PUE was the most comparable to GQD,with 24 consistent pathways.BAI and BER showed comparable gut microbiota regulation pathways.Characteristic pathways of BER include glucometabolic processes.Conclusion:This study focused on the key issues in the gut microbiota pathway and developed a progressive research strategy to understand the transformation mechanisms of colonic components.This research systematically analyzed the active components and metabolic transformation of GQD in the colon during the pathological state of UC,as well as changes in the structure and function of the gut microbiota,clarified the mechanism of GQD and its active components in improving UC via the gut microbiota pathway.展开更多
基金the National Key Research and Development Program of China(No.2023YFD2200903)the World Bank Loans Qiandao Lake and Xin’an River Basin Water Resources and Ecological Protection Projects in Zhejiang(CLJY3),China.
文摘Objectives:The chemical constituents of Poria cocos grown with different substrates vary significantly;thus,identifying and comparing their biomarkers are important.Materials and Methods:Herein,the chemical constituents of Poria cocos obtained with five different substrates were assessed using gas chromatography–ion mobility spectrometry(GC-IMS),high-performance liquid chromatography and multivariate statistical analysis.Results:The content of moisture,ash,alcohol-soluble matter,and heavy metals,except for those of the miscellaneous wood Poria cocos,conform to the specifications defined in the Chinese Pharmacopoeia(Edition 2020),and the polysaccharide contents are all greater than 57%.Conclusions:Based on GC-IMS and the established fingerprints,87 compounds were detected,70 of which were identified in each group.Multivariate statistical analysis revealed seven compounds(two esters,three alcohols,and two aldehydes),which could be considered as potential marker compounds for discrimination.
基金supported by Fundamental Research Funds for the Central Universities(2022-ZXFZJJ-028).
文摘Objective:To establish a progressive research strategy for“colonic components analysis-efficacy verification and mechanism exploration-gut microbiota”,screen pharmacodynamic substances,and investigate their mechanism via gut microbiota.Methods:The pharmacodynamics of Gegen Qinlian decoction(GQD)were assessed using a mouse model of dextran sulfate sodium-induced ulcerative colitis(UC).Ultra-performance liquid chromatographyquadrupole-orbitrap mass spectrometer was used to identify the prototype and metabolic components of GQD in the colon during UC.To analyze the structure and function of characteristic genera of GQD and its active components,16S rRNA sequencing was performed.Results:We identified 67 prototypic and 14 metabolic components of GQD in the UC colon.The primary prototype components are flavonoids and alkaloids,including puerarin(PUE),baicalin(BAI),and berberine(BER).The metabolism was predominantly sulfonation.Efficacy verification showed that the main active components,puerarin,baicalin,and berberine,had good therapeutic effects on UC.The results of 16S rRNA gene sequencing showed that GQD improved UC by regulating the structure and function of the gut microbiota.The abundance of gut microbiota involved in the metabolism of the prototype componentswas influenced by the corresponding components.The function prediction results showed that PUE was the most comparable to GQD,with 24 consistent pathways.BAI and BER showed comparable gut microbiota regulation pathways.Characteristic pathways of BER include glucometabolic processes.Conclusion:This study focused on the key issues in the gut microbiota pathway and developed a progressive research strategy to understand the transformation mechanisms of colonic components.This research systematically analyzed the active components and metabolic transformation of GQD in the colon during the pathological state of UC,as well as changes in the structure and function of the gut microbiota,clarified the mechanism of GQD and its active components in improving UC via the gut microbiota pathway.
