家蚕性信息素的生物合成受到信息素合成激活肽(pheromonebiosynthsis active neuropeptide,PBAN)的调控,羽化前,乙酰辅酶A(acetyl—Co A)通过脂肪酸生物合成的作用形成蚕蛾醇前体脂肪酸,蚕蛾醇前体以甘油三酯(Triacylglycerol,TAG)的形...家蚕性信息素的生物合成受到信息素合成激活肽(pheromonebiosynthsis active neuropeptide,PBAN)的调控,羽化前,乙酰辅酶A(acetyl—Co A)通过脂肪酸生物合成的作用形成蚕蛾醇前体脂肪酸,蚕蛾醇前体以甘油三酯(Triacylglycerol,TAG)的形式储存在信息素腺体(pheromone gland,PG)细胞质脂滴中。羽化后,PBAN与PG细胞表面的信息素合成激活肽受体(pheromonebiosynthsis active neuropeptide receptor,PBANR)结合,促进细胞外钙离子内流,随后钙离子依赖的钙调蛋白直接或间接激活磷蛋白磷酸酶,通过磷酸酶介导的磷酸化/去磷酸化激活脂解和脂肪酰基还原等过程,最终形成蚕蛾醇产物。在性信息素合成过程中有很多PG特异性表达的基因(Bmpgdesatl、pgFAR、BmFATP、pgACBP和mgACBP)起着重要的作用。本文主要对这几个关键性基因进行概述,希望能够促进对家蚕性信息素生物合成与释放过程分子机制的理解。展开更多
In moths,various enzymes,such as fatty acid synthases,fatty acyl desaturases,and fatty acyl reductases(FARs),are involved in pheromone biosynthesis.In particular,pheromone gland-specific FAR(pgFAR)plays an important r...In moths,various enzymes,such as fatty acid synthases,fatty acyl desaturases,and fatty acyl reductases(FARs),are involved in pheromone biosynthesis.In particular,pheromone gland-specific FAR(pgFAR)plays an important role in converting the functional group from carboxylic to alcohol during pheromone biosynthesis.A novel pgFAR of Maruca vitrata,Mvi-pgFAR,was identified through transcriptome sequencing of its pheromone gland.To investigate the involvement of Mvi-pgFAR in pheromone biosynthesis,Mvi-pgFAR was cloned from the pheromone gland and suppressed by RNA interference(RNAi).Mvi-pgFAR harbored several conserved motifs related to NAD(P)H-binding,N-glycosylation,and adenosine/guanosine triphosphate binding.Phylogenetic analysis revealed that Mvi-pgFAR with other lepidopteran pgFARs formed an independent clade.Mvi-pgFAR was specifically expressed only in the pheromone gland.Quantitative real-time polymerase chain reaction showed that the diurnal expression levels of Mvi-pgFAR in the pheromone gland were the highest at 2 h before the scotophase.After primarily confirming Mvi-pgFAR suppression by RNAi,(E,E)-10,12-hexadecadienal(E10E12-16:Ald),a major sex pheromone component,was quantified by gas chromatography.When Mvi-pgFAR was successfully suppressed,E10E12-16:Ald production was reduced by up to half of that of the control,and the mating rate was subsequently decreased.Our results demonstrate that Mvi-pgFAR downregulation can suppress mating behavior by changing the relative sex pheromone component ratio,suggesting that Mvi-pgFAR can be used as a novel control target.展开更多
文摘家蚕性信息素的生物合成受到信息素合成激活肽(pheromonebiosynthsis active neuropeptide,PBAN)的调控,羽化前,乙酰辅酶A(acetyl—Co A)通过脂肪酸生物合成的作用形成蚕蛾醇前体脂肪酸,蚕蛾醇前体以甘油三酯(Triacylglycerol,TAG)的形式储存在信息素腺体(pheromone gland,PG)细胞质脂滴中。羽化后,PBAN与PG细胞表面的信息素合成激活肽受体(pheromonebiosynthsis active neuropeptide receptor,PBANR)结合,促进细胞外钙离子内流,随后钙离子依赖的钙调蛋白直接或间接激活磷蛋白磷酸酶,通过磷酸酶介导的磷酸化/去磷酸化激活脂解和脂肪酰基还原等过程,最终形成蚕蛾醇产物。在性信息素合成过程中有很多PG特异性表达的基因(Bmpgdesatl、pgFAR、BmFATP、pgACBP和mgACBP)起着重要的作用。本文主要对这几个关键性基因进行概述,希望能够促进对家蚕性信息素生物合成与释放过程分子机制的理解。
基金This research was supported by Kyungsung University Research Grants(Project number 2019086)in 2019.
文摘In moths,various enzymes,such as fatty acid synthases,fatty acyl desaturases,and fatty acyl reductases(FARs),are involved in pheromone biosynthesis.In particular,pheromone gland-specific FAR(pgFAR)plays an important role in converting the functional group from carboxylic to alcohol during pheromone biosynthesis.A novel pgFAR of Maruca vitrata,Mvi-pgFAR,was identified through transcriptome sequencing of its pheromone gland.To investigate the involvement of Mvi-pgFAR in pheromone biosynthesis,Mvi-pgFAR was cloned from the pheromone gland and suppressed by RNA interference(RNAi).Mvi-pgFAR harbored several conserved motifs related to NAD(P)H-binding,N-glycosylation,and adenosine/guanosine triphosphate binding.Phylogenetic analysis revealed that Mvi-pgFAR with other lepidopteran pgFARs formed an independent clade.Mvi-pgFAR was specifically expressed only in the pheromone gland.Quantitative real-time polymerase chain reaction showed that the diurnal expression levels of Mvi-pgFAR in the pheromone gland were the highest at 2 h before the scotophase.After primarily confirming Mvi-pgFAR suppression by RNAi,(E,E)-10,12-hexadecadienal(E10E12-16:Ald),a major sex pheromone component,was quantified by gas chromatography.When Mvi-pgFAR was successfully suppressed,E10E12-16:Ald production was reduced by up to half of that of the control,and the mating rate was subsequently decreased.Our results demonstrate that Mvi-pgFAR downregulation can suppress mating behavior by changing the relative sex pheromone component ratio,suggesting that Mvi-pgFAR can be used as a novel control target.
