Gastric cancer(GC)is characterized by high morbidity and mortality rates.Chinese agarwood comprises the resin-containing wood of Aquilaria sinensis(Lour.)Gilg.,traditionally utilized for treating asthma,cardiac ischem...Gastric cancer(GC)is characterized by high morbidity and mortality rates.Chinese agarwood comprises the resin-containing wood of Aquilaria sinensis(Lour.)Gilg.,traditionally utilized for treating asthma,cardiac ischemia,and tumors.However,comprehensive research regarding its anti-GC effects and underlying mechanisms remains limited.In this study,Chinese agarwood petroleum ether extract(CAPEE)demonstrated potent cytotoxicity against human GC cells,with half maximal inhibitory concentration(IC_(50))values for AGS,HGC27,and MGC803 cells of 2.89,2.46,and 2.37μg·mL^(−1),respectively,at 48 h.CAPEE significantly induced apoptosis in these GC cells,with B-cell lymphoma-2(BCL-2)associated X protein(BAX)/BCL-2 antagonist killer 1(BAK)likely mediating CAPEE-induced apoptosis.Furthermore,CAPEE induced G_(0)/G_(1)phase cell cycle arrest in human GC cells via activation of the deoxyribonucleic acid(DNA)damage-p21-cyclin D1/cyclin-dependent kinase 4(CDK4)signaling axis,and increased Fe^(2+),lipid peroxides and reactive oxygen species(ROS)levels,thereby inducing ferroptosis.Ribonucleic acid(RNA)sequencing,real-time quantitative polymerase chain reaction(RT-qPCR),and Western blotting analyses revealed CAPEE-mediated upregulation of heme oxygenase-1(HO-1)in human GC cells.RNA interference studies demonstrated that HO-1 knockdown reduced CAPEE sensitivity and inhibited CAPEE-induced ferroptosis in human GC cells.Additionally,CAPEE administration exhibited robust in vivo anti-GC activity without significant toxicity in nude mice while inhibiting tumor cell growth and promoting apoptosis in tumor tissues.These findings indicate that CAPEE suppresses human GC cell growth through upregulation of the DNA damage-p21-cyclin D1/CDK4 signaling axis and HO-1-mediated ferroptosis,suggesting its potential as a candidate drug for GC treatment.展开更多
[Objective] This study aimed to investigate the effects of different irrigation modes on chemical constituent and aroma substance contents and sensory quality in flue-cured tobacco leaves. [Method] Field experiments w...[Objective] This study aimed to investigate the effects of different irrigation modes on chemical constituent and aroma substance contents and sensory quality in flue-cured tobacco leaves. [Method] Field experiments were conduced with two treatments, including watering irrigation and micro-spraying irrigation. In watering irrigation treatment, flue-cured tobacco seedlings were respectively watered by artificial root irrigation once at rosette stage（May 26） and vigorous growth stage（June 18）, 1kg/plant each time; in micro-spraying treatment, flue-cured tobacco seedlings were watered by artificial root irrigation once at rosette stage（May 26）, 1 L/plant. [Result]Compared to watering irrigation mode, micro-spraying irrigation could extremely significantly increase the content of main aromatic substance in middle and upper leaves.Contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, neophytadiene and neutral aromatic substances in C3F tobacco leaves were improved by 24.4%, 32.6%, 20.7%, 33.8% and 26.3%, respectively; contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, eophytadiene and neutral aromatic substances in B2F tobacco leaves were improved by 16.3%, 6.0%, 6.1%, 10.8% and8.1%, respectively. In addition, micro-spraying irrigation could extremely significantly reduce the nicotine content in middle and upper leaves and improve the aromatic coordination, mellow taste and smoldering property. [Conclusion] In flue-cured tobacco production, micro-spraying technology is an important means to improve the internal quality of flue-cured tobacco leaves, which can be adopted to effectively solve the problem of declining quality of flue-cured tobacco leaves resulted from the dry climate at maturation stage in seasonal frequent-drought areas.展开更多
Objective:To investigate whether Ershen Pill(ESP,二神丸)could alleviate the symptom of Pi(Spleen)-Shen(Kidney)yang deficiency(PSYD)-induced diarrhea in rat model and explore its anti-diarrhea mechanism.Methods:Seventy...Objective:To investigate whether Ershen Pill(ESP,二神丸)could alleviate the symptom of Pi(Spleen)-Shen(Kidney)yang deficiency(PSYD)-induced diarrhea in rat model and explore its anti-diarrhea mechanism.Methods:Seventy-five Sprague-Dawley rats were divided into 5 groups by a random number table,including control,positive,model,low-dose(LD)and high-dose(HD)ESP groups,15 rats in each group.All the rats,except those in the control group,were developed PSYD induced-diarrhea based on its pathology and etiology.The rats in positive,LD and HD ESP groups were treated with Shenling Baizhu Pill(参苓白术丸),LD(1.05 g/kg)or HD(3.50 g/kg)ESP petroleum ether extract once a day for 2 weeks,respectively.Body weight change and diarrhea index were measured.The histology scores of the kidney were evaluated via hematoxylin and eosin(HE)staining.Aquaporin-3(AQP3)expression in the colon was analyzed by immunofluorescence,quantitative reverse transcription-polymerase chain reaction(qRT-PCR)and Western blot,respectively.Results:Compared with the model group,oral administration of LD and HD ESP prevented body weight loss and inhibited diarrhea after 2-week treatment(P<0.05).Kidney deterioration was impeded,and the histology score in LD and HD ESP groups were 8.2 and 10.5,respectively,which were both higher than those in the model group(P<0.05).In addition,ESP treatment alleviated rat colitis,and HD ESP significantly improved the AQP3 positive staining intensity in the colon tissue compared with the model group.The result from Western blot revealed that AQP3 protein synthesis in colon tissue of LD and HD ESP groups increased by 2.1-and 5.9-fold compared with the model group(P<0.05).qR T-PCR result showed that AQP3 gene expression in the HD ESP group was also up-regulated by 2.5-fold normalized to the model group(P<0.05).Conclusion:ESP extract effectively alleviates the symptoms of PSYD and relieves PSYD-induced diarrhea by improving AQP3 synthesis in the colon.展开更多
基金supported by the Natural Science Foundation of Beijing City(No.J230034)the Fundamental Research Funds for the Central Universities(No.2023-JYB-JBQN-051)the Talent Cultivation Project of Beijing University of Chinese Medicine(No.JZPY202206).
文摘Gastric cancer(GC)is characterized by high morbidity and mortality rates.Chinese agarwood comprises the resin-containing wood of Aquilaria sinensis(Lour.)Gilg.,traditionally utilized for treating asthma,cardiac ischemia,and tumors.However,comprehensive research regarding its anti-GC effects and underlying mechanisms remains limited.In this study,Chinese agarwood petroleum ether extract(CAPEE)demonstrated potent cytotoxicity against human GC cells,with half maximal inhibitory concentration(IC_(50))values for AGS,HGC27,and MGC803 cells of 2.89,2.46,and 2.37μg·mL^(−1),respectively,at 48 h.CAPEE significantly induced apoptosis in these GC cells,with B-cell lymphoma-2(BCL-2)associated X protein(BAX)/BCL-2 antagonist killer 1(BAK)likely mediating CAPEE-induced apoptosis.Furthermore,CAPEE induced G_(0)/G_(1)phase cell cycle arrest in human GC cells via activation of the deoxyribonucleic acid(DNA)damage-p21-cyclin D1/cyclin-dependent kinase 4(CDK4)signaling axis,and increased Fe^(2+),lipid peroxides and reactive oxygen species(ROS)levels,thereby inducing ferroptosis.Ribonucleic acid(RNA)sequencing,real-time quantitative polymerase chain reaction(RT-qPCR),and Western blotting analyses revealed CAPEE-mediated upregulation of heme oxygenase-1(HO-1)in human GC cells.RNA interference studies demonstrated that HO-1 knockdown reduced CAPEE sensitivity and inhibited CAPEE-induced ferroptosis in human GC cells.Additionally,CAPEE administration exhibited robust in vivo anti-GC activity without significant toxicity in nude mice while inhibiting tumor cell growth and promoting apoptosis in tumor tissues.These findings indicate that CAPEE suppresses human GC cell growth through upregulation of the DNA damage-p21-cyclin D1/CDK4 signaling axis and HO-1-mediated ferroptosis,suggesting its potential as a candidate drug for GC treatment.
基金Supported by Science and Technology Project of Yunnan Zhongyan Industry Co.,Ltd.(2010YL01-2)Science and Technology Project of Hongyun Honghe Tobacco(Group) Co.Ltd.(HYHH2012YL01)~~
文摘[Objective] This study aimed to investigate the effects of different irrigation modes on chemical constituent and aroma substance contents and sensory quality in flue-cured tobacco leaves. [Method] Field experiments were conduced with two treatments, including watering irrigation and micro-spraying irrigation. In watering irrigation treatment, flue-cured tobacco seedlings were respectively watered by artificial root irrigation once at rosette stage（May 26） and vigorous growth stage（June 18）, 1kg/plant each time; in micro-spraying treatment, flue-cured tobacco seedlings were watered by artificial root irrigation once at rosette stage（May 26）, 1 L/plant. [Result]Compared to watering irrigation mode, micro-spraying irrigation could extremely significantly increase the content of main aromatic substance in middle and upper leaves.Contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, neophytadiene and neutral aromatic substances in C3F tobacco leaves were improved by 24.4%, 32.6%, 20.7%, 33.8% and 26.3%, respectively; contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, eophytadiene and neutral aromatic substances in B2F tobacco leaves were improved by 16.3%, 6.0%, 6.1%, 10.8% and8.1%, respectively. In addition, micro-spraying irrigation could extremely significantly reduce the nicotine content in middle and upper leaves and improve the aromatic coordination, mellow taste and smoldering property. [Conclusion] In flue-cured tobacco production, micro-spraying technology is an important means to improve the internal quality of flue-cured tobacco leaves, which can be adopted to effectively solve the problem of declining quality of flue-cured tobacco leaves resulted from the dry climate at maturation stage in seasonal frequent-drought areas.
基金Supported by the National Natural Science Foundation of China(No.81274085)
文摘Objective:To investigate whether Ershen Pill(ESP,二神丸)could alleviate the symptom of Pi(Spleen)-Shen(Kidney)yang deficiency(PSYD)-induced diarrhea in rat model and explore its anti-diarrhea mechanism.Methods:Seventy-five Sprague-Dawley rats were divided into 5 groups by a random number table,including control,positive,model,low-dose(LD)and high-dose(HD)ESP groups,15 rats in each group.All the rats,except those in the control group,were developed PSYD induced-diarrhea based on its pathology and etiology.The rats in positive,LD and HD ESP groups were treated with Shenling Baizhu Pill(参苓白术丸),LD(1.05 g/kg)or HD(3.50 g/kg)ESP petroleum ether extract once a day for 2 weeks,respectively.Body weight change and diarrhea index were measured.The histology scores of the kidney were evaluated via hematoxylin and eosin(HE)staining.Aquaporin-3(AQP3)expression in the colon was analyzed by immunofluorescence,quantitative reverse transcription-polymerase chain reaction(qRT-PCR)and Western blot,respectively.Results:Compared with the model group,oral administration of LD and HD ESP prevented body weight loss and inhibited diarrhea after 2-week treatment(P<0.05).Kidney deterioration was impeded,and the histology score in LD and HD ESP groups were 8.2 and 10.5,respectively,which were both higher than those in the model group(P<0.05).In addition,ESP treatment alleviated rat colitis,and HD ESP significantly improved the AQP3 positive staining intensity in the colon tissue compared with the model group.The result from Western blot revealed that AQP3 protein synthesis in colon tissue of LD and HD ESP groups increased by 2.1-and 5.9-fold compared with the model group(P<0.05).qR T-PCR result showed that AQP3 gene expression in the HD ESP group was also up-regulated by 2.5-fold normalized to the model group(P<0.05).Conclusion:ESP extract effectively alleviates the symptoms of PSYD and relieves PSYD-induced diarrhea by improving AQP3 synthesis in the colon.
