Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which ...Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which the impacts of feed type, water temperature, and light intensity, on the efficiency of its reproduction, were revealed. The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency. Higher reproductive efficiency [number of eggs laid （276±22.3 eggs）] was observed when fish were fed live Acetes sp. rather than clam （204±16.4 eggs）, trash fish （155±12 eggs） and formulated feed （110±10 eggs）. The spawning rate was increased during September and October （water temperature, 28.74±0.55℃） on average of 2.4 spawning per month; and low spawning rate was in January （water temperature, 24.55±0.45℃） on average of I spawning per month. Among three light intensities （100, 500, and 900 1x） set to evaluate larval survival rate, larvae showed the highest survival rate （65.5%） at 900 lx. The breeding method specifically in brackishwater developed in the present study is a new approach, will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat.展开更多
Objective:To study the lymphocystis disease virus(LCDV)in two species of marine ormamental fishes through histopathological investigation along with control for differentiating the tissue damage.Methods:Six naturally ...Objective:To study the lymphocystis disease virus(LCDV)in two species of marine ormamental fishes through histopathological investigation along with control for differentiating the tissue damage.Methods:Six naturally infected fishes were collected.They were anaesthetized and subsequently killed by organ dissection.The infected fish organs were aseptically cut off and stored with 10%formalin solution for histological study.Samples were examined for gross pathology including location,distribution,shape,size,colour,consistency and special features of typical external lesions by standard method.Results:The diameter of the tumor nodules ranged from 1 to 2 mm(Amphiprion ocellaris)and 2.5 to 3.5 mm(Amphiprion percula).Light microscopic observation showed over growth of tumor like nodules on the skin or scales and ventral side as well.Numerous hy pertrophied cells with basophilic intracytoplasmic inclusion bodies were in the connective tissues of dermis and between scales on two species.The nucleus of lymphocystis cell were enlarged,irregular and containing basophilic marginated chromatin.Thus,the similarities among cellular patterns of different fish LCDV isolates from different hosts indicated that these profiles do not depend on the host species.Conclusions:In conclusion,this current study of histopathological statement of LCDV may be adequate for a presumptive diagnosis of lymphocystis disease from both marine as well as fresh water fish species.The findings of asymptomatic carriers by histology using infected skin and fin sampling,which does not imply animal killing,could be important tool to epizootics caused by LCDV.This study may be very useful for further molecular studies.展开更多
文摘Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which the impacts of feed type, water temperature, and light intensity, on the efficiency of its reproduction, were revealed. The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency. Higher reproductive efficiency [number of eggs laid （276±22.3 eggs）] was observed when fish were fed live Acetes sp. rather than clam （204±16.4 eggs）, trash fish （155±12 eggs） and formulated feed （110±10 eggs）. The spawning rate was increased during September and October （water temperature, 28.74±0.55℃） on average of 2.4 spawning per month; and low spawning rate was in January （water temperature, 24.55±0.45℃） on average of I spawning per month. Among three light intensities （100, 500, and 900 1x） set to evaluate larval survival rate, larvae showed the highest survival rate （65.5%） at 900 lx. The breeding method specifically in brackishwater developed in the present study is a new approach, will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat.
基金Supported by Center for Marine Living Resource and Ecology,Ministry of Earth Sciences(CMLRE-Office Memorandum No:G4/3366/2013).
文摘Objective:To study the lymphocystis disease virus(LCDV)in two species of marine ormamental fishes through histopathological investigation along with control for differentiating the tissue damage.Methods:Six naturally infected fishes were collected.They were anaesthetized and subsequently killed by organ dissection.The infected fish organs were aseptically cut off and stored with 10%formalin solution for histological study.Samples were examined for gross pathology including location,distribution,shape,size,colour,consistency and special features of typical external lesions by standard method.Results:The diameter of the tumor nodules ranged from 1 to 2 mm(Amphiprion ocellaris)and 2.5 to 3.5 mm(Amphiprion percula).Light microscopic observation showed over growth of tumor like nodules on the skin or scales and ventral side as well.Numerous hy pertrophied cells with basophilic intracytoplasmic inclusion bodies were in the connective tissues of dermis and between scales on two species.The nucleus of lymphocystis cell were enlarged,irregular and containing basophilic marginated chromatin.Thus,the similarities among cellular patterns of different fish LCDV isolates from different hosts indicated that these profiles do not depend on the host species.Conclusions:In conclusion,this current study of histopathological statement of LCDV may be adequate for a presumptive diagnosis of lymphocystis disease from both marine as well as fresh water fish species.The findings of asymptomatic carriers by histology using infected skin and fin sampling,which does not imply animal killing,could be important tool to epizootics caused by LCDV.This study may be very useful for further molecular studies.
