In this study,the peanut peptide-selenium complex(PP-Se)was prepared and its structure,stability and anti-oxidant properties were thoroughly investigated.Structural characterization by^(1)H Nuclear Magnetic Resonance(...In this study,the peanut peptide-selenium complex(PP-Se)was prepared and its structure,stability and anti-oxidant properties were thoroughly investigated.Structural characterization by^(1)H Nuclear Magnetic Resonance(^(1)H NMR)spectroscopy,scanning electron microscopy(SEM),and atomic force microscopy(AFM)indicated that PP-Se is a novel complex that is different from peanut peptide(PP),in which the carboxyl and aminogroups of PP participate in the binding with SeO_(3)^(2-).PP-Se exhibited excellent stability under various conditions,including high temperature,acidic and alkaline environments,and alcohol solubility,and maintained a high selenium retention rate(>80%)during simulated gastrointestinal digestion.Antioxidant analysis showed that when the sample concentration was 10.0 mg/mL,compared with PP,the scavenging rate of 2,2′-Azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS)radicals,hydroxyl radicals and superoxide anion of PP-Se increased by 7.53%,57.60%and 41.12%,respectively,and the reducing power increased by 15.79%.In the HepG2 cell model with oxidative damage,PP-Se with the concentration of 75~125μg/mL showed low cytotoxicity(cell viability>90%)and effectively alleviated oxidative stress.Sample(100μg/mL)of PP-Se significantly reduced lactate dehydrogenase(LDH)release and malondialdehyde(MDA)content,while enhancing superoxide dismutase(SOD)and catalase(CAT)activities.This study provides a theoretical basis for the high-value utilization of peanut meal and the development of novel selenium supplements.展开更多
基金supported by National Natural Science Foundation of China(32472289,31871748)the Open Project Program of National Engineering Research Center of Wheat and Corn Further Processing,Henan University of Technology(NL2024008)+3 种基金Natural Science Foundation of Henan Province(242300421317)Cultivation Project for National Natural Science Foundation of Henan University of Technology(2024PYJH003)Zhengzhou Science and Technology Collaborative Innovation Project(21ZZXTCX17)Cultivation Project of Tuoxin Team in Henan University of Technology(2024TXTD04).
文摘In this study,the peanut peptide-selenium complex(PP-Se)was prepared and its structure,stability and anti-oxidant properties were thoroughly investigated.Structural characterization by^(1)H Nuclear Magnetic Resonance(^(1)H NMR)spectroscopy,scanning electron microscopy(SEM),and atomic force microscopy(AFM)indicated that PP-Se is a novel complex that is different from peanut peptide(PP),in which the carboxyl and aminogroups of PP participate in the binding with SeO_(3)^(2-).PP-Se exhibited excellent stability under various conditions,including high temperature,acidic and alkaline environments,and alcohol solubility,and maintained a high selenium retention rate(>80%)during simulated gastrointestinal digestion.Antioxidant analysis showed that when the sample concentration was 10.0 mg/mL,compared with PP,the scavenging rate of 2,2′-Azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS)radicals,hydroxyl radicals and superoxide anion of PP-Se increased by 7.53%,57.60%and 41.12%,respectively,and the reducing power increased by 15.79%.In the HepG2 cell model with oxidative damage,PP-Se with the concentration of 75~125μg/mL showed low cytotoxicity(cell viability>90%)and effectively alleviated oxidative stress.Sample(100μg/mL)of PP-Se significantly reduced lactate dehydrogenase(LDH)release and malondialdehyde(MDA)content,while enhancing superoxide dismutase(SOD)and catalase(CAT)activities.This study provides a theoretical basis for the high-value utilization of peanut meal and the development of novel selenium supplements.
