[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigo...[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigor index,peroxidase(POD) activity,superoxide dismutase(SOD) activity and malondialdehyde(MDA) content for four sunflower germplasms such as'SunM20','Deep Purple Minle','Da San Kui Hua 4'and'Ji Kui 24'were studied under high temperature and high humidity conditions(100% RH,45 ℃) for different days(0 d,2 d,4 d,6 d,8 d,10 d).[Result]The result showed that the germination capacity,vigor of germination,germination index,vigor index,POD activity,SOD activity declined gradually with the increase of seed aging days,whereas MDA content enhanced by degrees;The diggerences of resistance to artifical aging existed among the four accessions,'SunM20'was the most resistant one,and exhibited the strongest seed vigor,highest activities of two protective enzyme(POD,SOD) and lowest content of MDA at the uniform condition,moreover,the seed vigor and protective enzyme activities of'SunM20'changed slowest among the four materials during the aging process.[Conclusion]The distinct reduction of POD,SOD activities maybe the main reasons for the decrease of sunflower seed vigor at the artificial aging,and the gradual accumulation of a few MDA accelerated seeds aging.展开更多
A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being high...A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase (SOD) activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde (MDA) content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.展开更多
Two-line super hybrid rice (Oryza sativa L.) Guiliangyou 2 was taken as the experimental variety, the growth and physiological characteristics of rice under conventional tillage and smashing ridge tillage were compa...Two-line super hybrid rice (Oryza sativa L.) Guiliangyou 2 was taken as the experimental variety, the growth and physiological characteristics of rice under conventional tillage and smashing ridge tillage were compared based on field re- search. The results showed that smashing ridge tillage was beneficial to the tillering growth of rice plants in the the middle and later periods of tillering; under the tillage mode, the white root was more, the vigour was strong during the whole growing period, the SPAD was extremely significantly higher than that of conventional tillage, the net photosynthetic rate of the leaves was high and held green long, which were beneficial to the production and accumulation of photoassimilates; during mature pe- riod, the dry matter accumulation of the overground part under smashing ridge tillage was significantly higher than that of conventional tillage, and the number of productive ears under smashing ridge tillage was more than that of conventional tillage by 27.6×10^4/hm2, thus, the yield-increasing effect was significant and in- creased by 20.36%.展开更多
The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness...The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.展开更多
With the new pepper cultivar Guanlong as a test material, effects of differ-ent fertilizer application modes on growth and development, yield and photosynthetic characteristics of pepper were studied. The results show...With the new pepper cultivar Guanlong as a test material, effects of differ-ent fertilizer application modes on growth and development, yield and photosynthetic characteristics of pepper were studied. The results showed that the plant height and plant expansion of pepper in T4 were the largest, of 104.3 and 90.6 cm, respec-tively. T1 showed the largest weight of single fruit, fruit length and fruit diameter, re-spectively of 101.6 g, 25.49 cm and 4.86 cm. The content of total chlorophyl in the leaves of T1 was the highest, of 66.7 mg/g, and the root activity of T1 was also the highest, of 100.6 g/(g·FW·h). The net photosynthetic rate of pepper leaves in T5 was the highest, of 6.6 μmol/(m^2·s), while the net photosynthetic rates of T1 and T2 were the lowest, fluctuating in the range of 1.3-2.5 μmol/(m^2·s). T3 showed the highest decrease amplitude of stomatal conductance in pepper leaves, of 2 658.1 mol/(m^2·s), while the decrease amplitudes of stomatal conductance of pepper leaves in treatments T2 and T5 were the lowest, fluctuating in the range of 386.7-428.7 mol/(m^2·s). T1 showed the highest decrease amplitude of transpiration rate of pepper leaves, of 4.7 mmol/(m^2·s), while treatment T5 showed the lowest decrease ampli-tude of transpiration rate of pepper leaves, of 1.1 mmol/(m^2·s). The intercel ular CO2 concentration was the highest in the leaves of T5, of 436.8 μmol/mol. T1 showed the highest plot output, yield per hectare and benefit, of 340.1 kg, 96 448.5 kg/hm^2 and 162 865.6 Yuan/hm^2, respectively, while T3 exhibited the lowest plot output, yield per hectare and benefit, of 260.8 kg, 74 338.5 kg/hm^2 and 124 888.5 Yuan/hm^2, respec-tively.展开更多
The effects of different concentrations of NaCI (0, 0.10%, 0.25%, 0.40%, 0.55%, 0.70%) on the growth and physiological characteristics of three new varieties of Lagerstroemia indica were studied by pot experiment in...The effects of different concentrations of NaCI (0, 0.10%, 0.25%, 0.40%, 0.55%, 0.70%) on the growth and physiological characteristics of three new varieties of Lagerstroemia indica were studied by pot experiment in the plastic greenhouses. The results showed that under different NaCI concentrations, the relative height in- crement and the relative diameter increment of L. indica were restrained obviously; the contents of chlorophyll a, chlorophyll b, carotenoid and GSH decreased with in- crease of NaCI concentration; and the content of MDA increased with increase of NaCI concentration. Through the determination and comparison of physiological in- dexes, and analysis of correlativity, NaCI tolerance of the three new L. indica vari- eties ranked as L. indica 'Pink Velour'〉L. indica 'Dynamite'〉L. indica 'Red Rock- et'. This is consistent with the results of the morphological characteristics after Na- CI stress.展开更多
Field studies were conducted to examine the effects of cerium on the growth and physiological characteristics of Cyclocarya paliurus seedlings by spraying the foliage with different concentrations of cerium nitrate. O...Field studies were conducted to examine the effects of cerium on the growth and physiological characteristics of Cyclocarya paliurus seedlings by spraying the foliage with different concentrations of cerium nitrate. Optimal concentrations of cerium nitrate improved the relative growth yield of seedling height and stems and the soluble protein and sugar content of the leaves. Cerium nitrate also increased the concentration of secondary metabolites including triterpenoids, quercetin and kaempferol, mineral elements K, P, Mg, Mn, Fe and Cu, and the activities of superoxide dismutase (SOD), peroxidase (POX), and phenylalanine ammonia-lyase (PAL) in the leaves. The effects of cerium nitrate on these indices were dose dependent. A concentration of 0.20 mmol/L was optimal to promote the relative growth yield of seedling height, contents of soluble sugar, kaempferol, K, P, Cu, and activities of PAL, SOD, and POX, significantly increased by 54.61%, 14.71%, 55.19%, 105.2%, 74.5%, 133.3%, 80.48%, 25.35% and 22.54%, respectively, as compared with the control. However, the maximal increase in relative growth yield of stems, contents of triterpenoid, quercetin, Mg, Mn, and Fe was attained at 1.00 mmol/L treatment, which significantly increased by 87.00%, 80.56%, 452.44%, 93.2%, 29.4%, and 133.9%, respectively, compared with control check (CK). Correlation analysis revealed positive relationships between activities of PAL, SOD and contents of triterpenoid, quercetin and kaempferol within a certain concentration range of cerium nitrate. These resuits suggested that an appropriate concentration of cerium not only was effective in the improvement of physiological function of C. paliurus, but also increased seedling resistance. Moreover, it stimulated the synthesis of medicinal components in leaves.展开更多
[Objective] This study aimed to investigate the effects of phosphine on germination and physiological characteristics of rice seeds. [Method] Simulation envi- ronments were conducted to study the effects of high-level...[Objective] This study aimed to investigate the effects of phosphine on germination and physiological characteristics of rice seeds. [Method] Simulation envi- ronments were conducted to study the effects of high-level phosphine on germination status and physiological characteristics of rice seeds and explore the early environ- mental and ecological effects of phosphine on rice growth in phosphorus cycle of paddy field. [Result] Experimental results showed that the increase of phosphine con- centration in the environment resulted in the decrease of germination rate and ger- mination potential by 11.11% and 19.71%, respectively. In addition, the activities of catalase (CAT) and peroxidase (POD) were reduced to 94.35% and 92.61%, respec- tively; the content of malondialdehyde (MDA) was maximally increased by 29.11%, indicating that both germination potential and growth condition of rice seeds were in- hibited under conditions of high-level phosphine. [Conclusion] This study provided theoretical basis for investigating the effects of phosphine on germination of rice seeds under natural environment.展开更多
The purpose of this article is to study the effects of different water elevations of non-pressure subirrigation on some indexes of tomato, including soil water status around crop rootzone, morphological indexes, physi...The purpose of this article is to study the effects of different water elevations of non-pressure subirrigation on some indexes of tomato, including soil water status around crop rootzone, morphological indexes, physiological indexes, photosynthetic indexes, yield, quality, and water use efficiency. With the tomato materials of Dongsheng 1, the irrigation experiment was carried out in the greenhouse, and significance analysis was done on the experiment data through the software of DPS. The results showed that different water elevations, had significant influence on the growth, yield, and quality of tomato. The yield of the 6-cm treatment was the highest, the 3-cm treatment was inferior to the 6-cm treatment, and the 0-cm treatment was the lowest. However, the WUE was 0 cm 〉 3 cm 〉 6 cm. The sugar/acid and soluble protein was the highest under the 0-cm treatment, and the content of ascorbic acid did not decrease considerably. When compared to the 0-cm treatment, the ascorbic acid content of 6-cm and 3-cm treatment increased by 19.2 and 6.8%, respectively. These irrigation methods can satisfy the requirements of tomato growth; different water elevations have different influences on the tomato soil water status around crop rootzone, the physiological characteristics, and yield. It also harmonized the percentage between sugar and acid, increased the content of soluble protein and ascorbic acid, and made tomato more delicious. The irrigation methods can improve the quality of tomato by water control, which is worth promoting in the agricultural production.展开更多
Numerous growth and physiological variables of 3-week-old Populus simonii × P.nigra seedlings were assessed after treatment with either nitrate nitrogen(NO_(3)^(−)--N)(0.1,0.5,1,5,or 10 mmol·L^(−1)) or ammon...Numerous growth and physiological variables of 3-week-old Populus simonii × P.nigra seedlings were assessed after treatment with either nitrate nitrogen(NO_(3)^(−)--N)(0.1,0.5,1,5,or 10 mmol·L^(−1)) or ammonium nitrogen(NH_(4)^(+)+-N)(0.1,0.5,1,5,or 10 mmol·L^(−1)) to determine the best nitrogen form and concentration to optimize growth,biomass allocation,pigment content,and photosynthetic capacity.The results of combining membership function and an evaluation index suggested that,5 mmol·L^(−1) nitrogen,regardless of the form,yielded the highest comprehensive evaluation index and good growth.In addition,a Pearson correlation analysis and network visualization revealed that the total mass,shoot mass,root mass,leaf dry mass,plant height,leaf area,chlorophyll a and total chlorophyll had a physiological index connectivity degree≥15 for both nitrogen forms.Net photosynthetic rate,stomatal conductance,transpiration rate,maximum photochemical efficiency of PSII,total nitrogen content,ground diameter,chlorophyll b,and carotenoid were unique indices for evaluating NH_(4)^(+)+-N-based nutrition,which could provide a theoretical basis for evaluating the effects of nitrogen fertilizer on seedlings,cultivation periods,and stress tolerance in P.simonii× P.nigra.展开更多
As shown by the changes in physiological characteristics and seed vigor of three Medicago ruthenica samples during artificial aging process, the germination potential, germination rate, germination index, vigor index ...As shown by the changes in physiological characteristics and seed vigor of three Medicago ruthenica samples during artificial aging process, the germination potential, germination rate, germination index, vigor index and simple vigor index declined with the extension of aging time on the whole. After artificial aging for 6 min, the vigor was higher than that of the control. The conductivity, malondialdehyde content and soluble polysaccharide content of seed leachate increased with the aging time and negatively correlated ( P 〈 0.05) with the seed vigor indicators. The physiological indicators were very significantly correlated (P 〈 0.01 ) with the vigor indicators in the Medicago ruthenica sample from Dorbod Qi, UIanqab City(China).展开更多
In order to identify the resistance of peanut varieties against scab disease, the changes of physiological characteristics and yield in two peanut varieties 'Heyou 13' and 'Quanhual0' with different resistance eff...In order to identify the resistance of peanut varieties against scab disease, the changes of physiological characteristics and yield in two peanut varieties 'Heyou 13' and 'Quanhual0' with different resistance effected by peanut scab were stud- ied in field experiment by spray. The results showed the soluble sugar increased firstly and then decreased both in the resistant and susceptible peanuts after inocu- lation. Compared with the control, the soluble sugar of resistant peanut was higher, and that of the susceptible peanut was lower, which implied that the peanut resis- tance to scab was positive correlated with soluble sugar content. The soluble pro- tein content in leaves of resistant and susceptible peanut varieties after inoculation was higher than that of the control. The peak of soluble protein content appeared earlier in the susceptible peanut than in the resistant peanut, which indicated that the susceptible peanut were more sensitive to the pathogen than the resistant peanut. The leaf SOD activity increased firstly and then decreased both in resistant and susceptible varieties, while the decrease in the resistant variety were lower than in the susceptible variety. With the growth process, the CAT and POD activities first decreased, then increased, and again decreased. Compared with the corresponding control, the resistant variety had relatively higher CAT and POD activities than the susceptible variety, which illustrated that the protective enzymes played important roles in resistance mechanism to scab. The MDA content of the two peanut vari- eties after inoculation increased, indicating that plasma membranes were subjected to different degrees of damage. The yield of resistant and susceptible varieties after inoculation reduced, while the yield of the susceptible peanut of 'Quanhua 10' reduced greater.展开更多
[Objective] This study aimed to investigate the effects of traffic stress on the physiological characteristics of Agrostis stolonifera under high temperature and humidity in summer. [Method] Total 3 creeping bentgrass...[Objective] This study aimed to investigate the effects of traffic stress on the physiological characteristics of Agrostis stolonifera under high temperature and humidity in summer. [Method] Total 3 creeping bentgrass varieties (lines) were se- lected as materials, and their physiological characteristics were determined and ana- lyzed, including leaf relative water content, relative plasma membrane permeability, peroxidase activity, superoxide dismutase (SOD) activity, catalase activity, proline content and malondialdehyde (MDA) content. [Result] Under the traffic stress, the leaf relative water content, relative plasma membrane permeability, MDA content, proline content and protection enzymes activities showed significant differences among different traffic intensities and tested varieties (P〈0.05). The variations of various physiological indexes of Yuexuan 1 were smallest, and it showed relatively strong traffic tolerance. [Conclusion] Considering the traffic tolerance, the 3 tested creeping bentgrass varieties ranked as Yuexuan 1〉New Strain No.2〉Penncross.展开更多
The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of wate...The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of water was investigated. It established the reduction of seed germination both under direct and indirect effects due to water action of attenuated geomagnetic field. A negative effect of hypomagnetic field on grown characteristics of seeds under indirect effect via water was decreased by the low doses of γ-irradiation, and was increased by low doses of α-irradiation, i.e. ionized radiation was the dominant factor in their combined action. It was revealed the increasing of the value of the oxidation-reduction potential of water under the influence of low-intensive α-ir-radiation (239Pu), γ-irradiation (137Cs) and also that the magnetic induction attenuated pointing to a natural decline. The increasing of the oxidation-reduction potential value testifies about “the regular decreasing of internal energy of water molecules” and the increasing of its oxidative properties, which, in our opinion, is caused the inhibition of the germination of seeds. It is supposed that namely water is the main component in the effects of studying factors on bio-objects, which acts due to the alterations of the properties and structural content of water.展开更多
There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on micro...There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on microstructure, most reports are focused on the toxicology rather than promotion. Using cucumis sativus L. (Jin Chun No.5) as experiment material, we try to find out the nutritional effects of low Y3+ concentrations on cucumber seedlings′ leaves. The present paper suggests that the rare earth elements act as micronutrients at low concentrations while they give rise to toxicity at high concentration. Benefits defeat toxicity with concentration ranging from 5 to 25 μmol·L-1. Through careful study, at the Y(NO3)3 concentration of 10 μmol·L-1 the content of chlorophyll as well as the activities of SOD, Cu-Zn SOD and the POD are the highest. It indicates 10 μmol·L-1 is the optimum concentration of yttrium for promoting the cucumber growth.展开更多
In order to select the suitable alfalfa varieties for the shade areas in vineyard of Northwest, four shasing treatments Ro, R1 , R2, R3 ( full light, 60% of full light, 30% of full light, 10% of full light) were set...In order to select the suitable alfalfa varieties for the shade areas in vineyard of Northwest, four shasing treatments Ro, R1 , R2, R3 ( full light, 60% of full light, 30% of full light, 10% of full light) were set in the experiment to study the growth and physiological characteristics of seven domestic and foreign alfalfa varieties. The results showed that there was a big difference in height of alfalfa among cuhivars under shading; leaf area, leaf dry weight and leaf number reduced significantly with the decrease of light intensity ( P 〈 0.05 ) ; relative growth ratio, soluble sugar content, chlorophyll a/h decreased with the increase of shading in- tensity. The first harvest yield decreased significantly with the increasing shading intensity, AmeriStand and Queen declined less than others. In summary, the suit- able alfalfa cultivars for plantation were Queen, Alfalfa and AmeriStand in the northwest area where the habitat was similar with the habitat of experimental site, but due to the relativity of shading ability, the shading intensity could not exceed 50%.展开更多
This study aimed to investigate chlorophyll content and photosynthetic characteristics of 17 pepper inbred lines which were selected independently. The results showed that chlorophyll contents significantly varied amo...This study aimed to investigate chlorophyll content and photosynthetic characteristics of 17 pepper inbred lines which were selected independently. The results showed that chlorophyll contents significantly varied among different inbred lines. To be specific, chlorophyll content of No. 68 inbred line was significantly higher than that of other 14 inbred lines ; chlorophyll content of No. 55 inbred line was signifieandy lower than that of other 15 inbred lines. The chlorophyll content of various inbred lines showed a decreasing order of No. 68 〉 No. 47 〉 No. 66 〉 No. 62 〉 No. 63 〉 No. 60 〉 No. 61 〉 No. 64 〉 No. 56 〉 No. 67 〉 No. 65 〉 No. 48 〉 No. 59 〉 No. 58 〉 No. 57 〉 No. 69 〉 No. 55 ; the photosynthetic rate of various inbred lines showed a decreasing order of No. 68 〉 No. 57 〉 No. 60 〉 No. 48 〉 No. 58 〉 No. 47 〉 No. 62 〉 No. 63 〉 No. 64 〉 No. 65 ; the maximum photesynthetie rate of No. 65 inbred line was significantly lower than other seven inbred lines, while no signifi- cant difference was observed among other nine inbred lines.展开更多
The aim of the present study was to investigate the effect of electromagnetic radio frequency treatment of water on the growth of pepper (Capsicum annuum) plants. For this experiment, one hundred one-week old plants w...The aim of the present study was to investigate the effect of electromagnetic radio frequency treatment of water on the growth of pepper (Capsicum annuum) plants. For this experiment, one hundred one-week old plants were divided into two groups. The first group of plants was watered with water subjected to radio frequency electromagnetic radiation from an internet router for one hour a day, while the other group was watered with tap water (control). The overall results showed changes of growth characters of plant watered with electromagnetic water. The length of pepper plants is significantly affected by the treated water, where the length of shoot was lower in plants grown under the effect of treated water (22.43 ± 7.17 cm) than those grown without treated water (28.11 ± 8.57 cm). The results revealed that the stem diameter of control plants (1.74 ± 0.39 cm) was significantly higher than that of the treated plants (1.66 ± 0.35 cm). In addition, the root length was lower in plants grown under the effect of treated water than those grown without treated water. Pepper plants watered with electromagnetic treated water exhibited marked decreases in health index, fresh and dry weight, relative water content, number of flowers and fruits/plant as well as number of seeds/fruit. In addition, the current experiment showed a significant decrease in the number of leaves, branch and flower per plant when watered with electromagnetic treated water. The results revealed that the first flowering time for plants in treated group was remarkably decelerated when compared to other plants in control group.展开更多
Effective user authentication is key to ensuring equipment security,data privacy,and personalized services in Internet of Things(IoT)systems.However,conventional mode-based authentication methods(e.g.,passwords and sm...Effective user authentication is key to ensuring equipment security,data privacy,and personalized services in Internet of Things(IoT)systems.However,conventional mode-based authentication methods(e.g.,passwords and smart cards)may be vulnerable to a broad range of attacks(e.g.,eavesdropping and side-channel attacks).Hence,there have been attempts to design biometric-based authentication solutions,which rely on physiological and behavioral characteristics.Behavioral characteristics need continuous monitoring and specific environmental settings,which can be challenging to implement in practice.However,we can also leverage Artificial Intelligence(AI)in the extraction and classification of physiological characteristics from IoT devices processing to facilitate authentication.Thus,we review the literature on the use of AI in physiological characteristics recognition pub-lished after 2015.We use the three-layer architecture of the IoT(i.e.,sensing layer,feature layer,and algorithm layer)to guide the discussion of existing approaches and their limitations.We also identify a number of future research opportunities,which will hopefully guide the design of next generation solutions.展开更多
The research performed analysis on differences of physiological property and yield of Tiejing No. 11 by different nitrogen fertilization patterns. The results indicated that while the nitrogen of basal: tillering: pan...The research performed analysis on differences of physiological property and yield of Tiejing No. 11 by different nitrogen fertilization patterns. The results indicated that while the nitrogen of basal: tillering: panicle was 6 ∶3∶1, the higher amount of nitrogen improved the chlorophyll content, photosynthetic capacity, effective panicle, grain weight and yield; the medium nitrogen level had advantage under the ratio 4:3:3; nitrogen application at lategrowth stage was suitable for low or medium nitrogen level,which could increase photosynthetic capacity and optimize the yield components and increase yield; under the same ratio for basic tiller and ear fertilizer, the increase of nitrogen could improve total nitrogen uptake and protein content, decrease nitrogen physiological efficiency and milled rice and tasting, but nitrogen recovery and harvest index changes had difference; as application at late growth stage in same nitrogen rate, total nitrogen uptake and protein content increased, nitrogen physiological efficiency and milled rice and amylose content and tasting decreased, nitrogen recovery and harvest index increased under low or medium nitrogen level and decreased under high nitrogen level. Nitrogen application 210 kg/hm^2 and the ratio 4 ∶3 ∶3 of basal:tillering: panicle made the yield increase by 2.8%-11.3%, and this is the optimal mode of nitrogen fertilizer application.展开更多
文摘[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigor index,peroxidase(POD) activity,superoxide dismutase(SOD) activity and malondialdehyde(MDA) content for four sunflower germplasms such as'SunM20','Deep Purple Minle','Da San Kui Hua 4'and'Ji Kui 24'were studied under high temperature and high humidity conditions(100% RH,45 ℃) for different days(0 d,2 d,4 d,6 d,8 d,10 d).[Result]The result showed that the germination capacity,vigor of germination,germination index,vigor index,POD activity,SOD activity declined gradually with the increase of seed aging days,whereas MDA content enhanced by degrees;The diggerences of resistance to artifical aging existed among the four accessions,'SunM20'was the most resistant one,and exhibited the strongest seed vigor,highest activities of two protective enzyme(POD,SOD) and lowest content of MDA at the uniform condition,moreover,the seed vigor and protective enzyme activities of'SunM20'changed slowest among the four materials during the aging process.[Conclusion]The distinct reduction of POD,SOD activities maybe the main reasons for the decrease of sunflower seed vigor at the artificial aging,and the gradual accumulation of a few MDA accelerated seeds aging.
文摘A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase (SOD) activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde (MDA) content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.
基金Supported by the Special Project of Basic Scientific Research of Guangxi Academy of Agricultural Sciences(Gui Nong Ke 2013YM50)the Special Project of Basic Scientific Research of Guangxi Academy of Agricultural Sciences(Gui Nong Ke 2013YM43)the Planned Project of Guangxi Scientific Research and Technological Development(Gui Ke Neng 1346007-9)~~
文摘Two-line super hybrid rice (Oryza sativa L.) Guiliangyou 2 was taken as the experimental variety, the growth and physiological characteristics of rice under conventional tillage and smashing ridge tillage were compared based on field re- search. The results showed that smashing ridge tillage was beneficial to the tillering growth of rice plants in the the middle and later periods of tillering; under the tillage mode, the white root was more, the vigour was strong during the whole growing period, the SPAD was extremely significantly higher than that of conventional tillage, the net photosynthetic rate of the leaves was high and held green long, which were beneficial to the production and accumulation of photoassimilates; during mature pe- riod, the dry matter accumulation of the overground part under smashing ridge tillage was significantly higher than that of conventional tillage, and the number of productive ears under smashing ridge tillage was more than that of conventional tillage by 27.6×10^4/hm2, thus, the yield-increasing effect was significant and in- creased by 20.36%.
基金Supported by National Natural Science Foundation of China(Grant Nos.52375279,52175001)Beijing Natural Science Foundation(Grant No.3252002).
文摘The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.
基金Supported by National Staple Vegetables(CARS-25-G-50)~~
文摘With the new pepper cultivar Guanlong as a test material, effects of differ-ent fertilizer application modes on growth and development, yield and photosynthetic characteristics of pepper were studied. The results showed that the plant height and plant expansion of pepper in T4 were the largest, of 104.3 and 90.6 cm, respec-tively. T1 showed the largest weight of single fruit, fruit length and fruit diameter, re-spectively of 101.6 g, 25.49 cm and 4.86 cm. The content of total chlorophyl in the leaves of T1 was the highest, of 66.7 mg/g, and the root activity of T1 was also the highest, of 100.6 g/(g·FW·h). The net photosynthetic rate of pepper leaves in T5 was the highest, of 6.6 μmol/(m^2·s), while the net photosynthetic rates of T1 and T2 were the lowest, fluctuating in the range of 1.3-2.5 μmol/(m^2·s). T3 showed the highest decrease amplitude of stomatal conductance in pepper leaves, of 2 658.1 mol/(m^2·s), while the decrease amplitudes of stomatal conductance of pepper leaves in treatments T2 and T5 were the lowest, fluctuating in the range of 386.7-428.7 mol/(m^2·s). T1 showed the highest decrease amplitude of transpiration rate of pepper leaves, of 4.7 mmol/(m^2·s), while treatment T5 showed the lowest decrease ampli-tude of transpiration rate of pepper leaves, of 1.1 mmol/(m^2·s). The intercel ular CO2 concentration was the highest in the leaves of T5, of 436.8 μmol/mol. T1 showed the highest plot output, yield per hectare and benefit, of 340.1 kg, 96 448.5 kg/hm^2 and 162 865.6 Yuan/hm^2, respectively, while T3 exhibited the lowest plot output, yield per hectare and benefit, of 260.8 kg, 74 338.5 kg/hm^2 and 124 888.5 Yuan/hm^2, respec-tively.
文摘The effects of different concentrations of NaCI (0, 0.10%, 0.25%, 0.40%, 0.55%, 0.70%) on the growth and physiological characteristics of three new varieties of Lagerstroemia indica were studied by pot experiment in the plastic greenhouses. The results showed that under different NaCI concentrations, the relative height in- crement and the relative diameter increment of L. indica were restrained obviously; the contents of chlorophyll a, chlorophyll b, carotenoid and GSH decreased with in- crease of NaCI concentration; and the content of MDA increased with increase of NaCI concentration. Through the determination and comparison of physiological in- dexes, and analysis of correlativity, NaCI tolerance of the three new L. indica vari- eties ranked as L. indica 'Pink Velour'〉L. indica 'Dynamite'〉L. indica 'Red Rock- et'. This is consistent with the results of the morphological characteristics after Na- CI stress.
基金supported by National Training Programs of Innovation and Entrepreneurship for Undergraduates(201210298004)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Field studies were conducted to examine the effects of cerium on the growth and physiological characteristics of Cyclocarya paliurus seedlings by spraying the foliage with different concentrations of cerium nitrate. Optimal concentrations of cerium nitrate improved the relative growth yield of seedling height and stems and the soluble protein and sugar content of the leaves. Cerium nitrate also increased the concentration of secondary metabolites including triterpenoids, quercetin and kaempferol, mineral elements K, P, Mg, Mn, Fe and Cu, and the activities of superoxide dismutase (SOD), peroxidase (POX), and phenylalanine ammonia-lyase (PAL) in the leaves. The effects of cerium nitrate on these indices were dose dependent. A concentration of 0.20 mmol/L was optimal to promote the relative growth yield of seedling height, contents of soluble sugar, kaempferol, K, P, Cu, and activities of PAL, SOD, and POX, significantly increased by 54.61%, 14.71%, 55.19%, 105.2%, 74.5%, 133.3%, 80.48%, 25.35% and 22.54%, respectively, as compared with the control. However, the maximal increase in relative growth yield of stems, contents of triterpenoid, quercetin, Mg, Mn, and Fe was attained at 1.00 mmol/L treatment, which significantly increased by 87.00%, 80.56%, 452.44%, 93.2%, 29.4%, and 133.9%, respectively, compared with control check (CK). Correlation analysis revealed positive relationships between activities of PAL, SOD and contents of triterpenoid, quercetin and kaempferol within a certain concentration range of cerium nitrate. These resuits suggested that an appropriate concentration of cerium not only was effective in the improvement of physiological function of C. paliurus, but also increased seedling resistance. Moreover, it stimulated the synthesis of medicinal components in leaves.
基金Supported by National Natural Science Foundation of China(41071305)~~
文摘[Objective] This study aimed to investigate the effects of phosphine on germination and physiological characteristics of rice seeds. [Method] Simulation envi- ronments were conducted to study the effects of high-level phosphine on germination status and physiological characteristics of rice seeds and explore the early environ- mental and ecological effects of phosphine on rice growth in phosphorus cycle of paddy field. [Result] Experimental results showed that the increase of phosphine con- centration in the environment resulted in the decrease of germination rate and ger- mination potential by 11.11% and 19.71%, respectively. In addition, the activities of catalase (CAT) and peroxidase (POD) were reduced to 94.35% and 92.61%, respec- tively; the content of malondialdehyde (MDA) was maximally increased by 29.11%, indicating that both germination potential and growth condition of rice seeds were in- hibited under conditions of high-level phosphine. [Conclusion] This study provided theoretical basis for investigating the effects of phosphine on germination of rice seeds under natural environment.
基金This research was supported by the National Natural Science Foundation of China (50479051)the Agricultural Science and Technology Achievement Transformation Foundation of China (04EFN217100395)the Innovation Projects of Northwest A & F University for Graduate Students.
文摘The purpose of this article is to study the effects of different water elevations of non-pressure subirrigation on some indexes of tomato, including soil water status around crop rootzone, morphological indexes, physiological indexes, photosynthetic indexes, yield, quality, and water use efficiency. With the tomato materials of Dongsheng 1, the irrigation experiment was carried out in the greenhouse, and significance analysis was done on the experiment data through the software of DPS. The results showed that different water elevations, had significant influence on the growth, yield, and quality of tomato. The yield of the 6-cm treatment was the highest, the 3-cm treatment was inferior to the 6-cm treatment, and the 0-cm treatment was the lowest. However, the WUE was 0 cm 〉 3 cm 〉 6 cm. The sugar/acid and soluble protein was the highest under the 0-cm treatment, and the content of ascorbic acid did not decrease considerably. When compared to the 0-cm treatment, the ascorbic acid content of 6-cm and 3-cm treatment increased by 19.2 and 6.8%, respectively. These irrigation methods can satisfy the requirements of tomato growth; different water elevations have different influences on the tomato soil water status around crop rootzone, the physiological characteristics, and yield. It also harmonized the percentage between sugar and acid, increased the content of soluble protein and ascorbic acid, and made tomato more delicious. The irrigation methods can improve the quality of tomato by water control, which is worth promoting in the agricultural production.
基金This work was supported by the Science Fund Project of Heilongjiang Province of China(ZD2020C004)the Special Fund for Basic Scientifi c research operation Fee of Central University(2572019CT02)+1 种基金the Innovation Project of State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University)(2019A03)Heilongjiang Touyan Innovation Team Program(Tree Genetics and Breeding Innovation Team).
文摘Numerous growth and physiological variables of 3-week-old Populus simonii × P.nigra seedlings were assessed after treatment with either nitrate nitrogen(NO_(3)^(−)--N)(0.1,0.5,1,5,or 10 mmol·L^(−1)) or ammonium nitrogen(NH_(4)^(+)+-N)(0.1,0.5,1,5,or 10 mmol·L^(−1)) to determine the best nitrogen form and concentration to optimize growth,biomass allocation,pigment content,and photosynthetic capacity.The results of combining membership function and an evaluation index suggested that,5 mmol·L^(−1) nitrogen,regardless of the form,yielded the highest comprehensive evaluation index and good growth.In addition,a Pearson correlation analysis and network visualization revealed that the total mass,shoot mass,root mass,leaf dry mass,plant height,leaf area,chlorophyll a and total chlorophyll had a physiological index connectivity degree≥15 for both nitrogen forms.Net photosynthetic rate,stomatal conductance,transpiration rate,maximum photochemical efficiency of PSII,total nitrogen content,ground diameter,chlorophyll b,and carotenoid were unique indices for evaluating NH_(4)^(+)+-N-based nutrition,which could provide a theoretical basis for evaluating the effects of nitrogen fertilizer on seedlings,cultivation periods,and stress tolerance in P.simonii× P.nigra.
基金funded by National Key Technology R & DProgram during the 11th Five-Year Plan Period (2008BADB3B01)Crops Germplasm Resource Conservation Project of Ministry of Agriculture (NB08-2130135-43)
文摘As shown by the changes in physiological characteristics and seed vigor of three Medicago ruthenica samples during artificial aging process, the germination potential, germination rate, germination index, vigor index and simple vigor index declined with the extension of aging time on the whole. After artificial aging for 6 min, the vigor was higher than that of the control. The conductivity, malondialdehyde content and soluble polysaccharide content of seed leachate increased with the aging time and negatively correlated ( P 〈 0.05) with the seed vigor indicators. The physiological indicators were very significantly correlated (P 〈 0.01 ) with the vigor indicators in the Medicago ruthenica sample from Dorbod Qi, UIanqab City(China).
基金Supported by the Earmarked Fund for Modern Agroindustry Technology Research System of China(CARS-13)Key Project of Science and Technology Plan of Quanzhou(2012N76)~~
文摘In order to identify the resistance of peanut varieties against scab disease, the changes of physiological characteristics and yield in two peanut varieties 'Heyou 13' and 'Quanhual0' with different resistance effected by peanut scab were stud- ied in field experiment by spray. The results showed the soluble sugar increased firstly and then decreased both in the resistant and susceptible peanuts after inocu- lation. Compared with the control, the soluble sugar of resistant peanut was higher, and that of the susceptible peanut was lower, which implied that the peanut resis- tance to scab was positive correlated with soluble sugar content. The soluble pro- tein content in leaves of resistant and susceptible peanut varieties after inoculation was higher than that of the control. The peak of soluble protein content appeared earlier in the susceptible peanut than in the resistant peanut, which indicated that the susceptible peanut were more sensitive to the pathogen than the resistant peanut. The leaf SOD activity increased firstly and then decreased both in resistant and susceptible varieties, while the decrease in the resistant variety were lower than in the susceptible variety. With the growth process, the CAT and POD activities first decreased, then increased, and again decreased. Compared with the corresponding control, the resistant variety had relatively higher CAT and POD activities than the susceptible variety, which illustrated that the protective enzymes played important roles in resistance mechanism to scab. The MDA content of the two peanut vari- eties after inoculation increased, indicating that plasma membranes were subjected to different degrees of damage. The yield of resistant and susceptible varieties after inoculation reduced, while the yield of the susceptible peanut of 'Quanhua 10' reduced greater.
文摘[Objective] This study aimed to investigate the effects of traffic stress on the physiological characteristics of Agrostis stolonifera under high temperature and humidity in summer. [Method] Total 3 creeping bentgrass varieties (lines) were se- lected as materials, and their physiological characteristics were determined and ana- lyzed, including leaf relative water content, relative plasma membrane permeability, peroxidase activity, superoxide dismutase (SOD) activity, catalase activity, proline content and malondialdehyde (MDA) content. [Result] Under the traffic stress, the leaf relative water content, relative plasma membrane permeability, MDA content, proline content and protection enzymes activities showed significant differences among different traffic intensities and tested varieties (P〈0.05). The variations of various physiological indexes of Yuexuan 1 were smallest, and it showed relatively strong traffic tolerance. [Conclusion] Considering the traffic tolerance, the 3 tested creeping bentgrass varieties ranked as Yuexuan 1〉New Strain No.2〉Penncross.
文摘The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of water was investigated. It established the reduction of seed germination both under direct and indirect effects due to water action of attenuated geomagnetic field. A negative effect of hypomagnetic field on grown characteristics of seeds under indirect effect via water was decreased by the low doses of γ-irradiation, and was increased by low doses of α-irradiation, i.e. ionized radiation was the dominant factor in their combined action. It was revealed the increasing of the value of the oxidation-reduction potential of water under the influence of low-intensive α-ir-radiation (239Pu), γ-irradiation (137Cs) and also that the magnetic induction attenuated pointing to a natural decline. The increasing of the oxidation-reduction potential value testifies about “the regular decreasing of internal energy of water molecules” and the increasing of its oxidative properties, which, in our opinion, is caused the inhibition of the germination of seeds. It is supposed that namely water is the main component in the effects of studying factors on bio-objects, which acts due to the alterations of the properties and structural content of water.
基金Project supported by Scientific Grant of TianJin Normal University (1CHS02B)Special Grant of the President of TianJin Normal University
文摘There is no doubt that rare earth elements stand an important position among the essential elements of plant growth and it is long time since they are first used as plant growth promoters. Given their effects on microstructure, most reports are focused on the toxicology rather than promotion. Using cucumis sativus L. (Jin Chun No.5) as experiment material, we try to find out the nutritional effects of low Y3+ concentrations on cucumber seedlings′ leaves. The present paper suggests that the rare earth elements act as micronutrients at low concentrations while they give rise to toxicity at high concentration. Benefits defeat toxicity with concentration ranging from 5 to 25 μmol·L-1. Through careful study, at the Y(NO3)3 concentration of 10 μmol·L-1 the content of chlorophyll as well as the activities of SOD, Cu-Zn SOD and the POD are the highest. It indicates 10 μmol·L-1 is the optimum concentration of yttrium for promoting the cucumber growth.
基金Supported by GMKY 2014-12&GKG 1598006-3-12&GKG 15248003-21
文摘In order to select the suitable alfalfa varieties for the shade areas in vineyard of Northwest, four shasing treatments Ro, R1 , R2, R3 ( full light, 60% of full light, 30% of full light, 10% of full light) were set in the experiment to study the growth and physiological characteristics of seven domestic and foreign alfalfa varieties. The results showed that there was a big difference in height of alfalfa among cuhivars under shading; leaf area, leaf dry weight and leaf number reduced significantly with the decrease of light intensity ( P 〈 0.05 ) ; relative growth ratio, soluble sugar content, chlorophyll a/h decreased with the increase of shading in- tensity. The first harvest yield decreased significantly with the increasing shading intensity, AmeriStand and Queen declined less than others. In summary, the suit- able alfalfa cultivars for plantation were Queen, Alfalfa and AmeriStand in the northwest area where the habitat was similar with the habitat of experimental site, but due to the relativity of shading ability, the shading intensity could not exceed 50%.
基金Supported by vegetable Station of Ningxia Hui Autonomous Region
文摘This study aimed to investigate chlorophyll content and photosynthetic characteristics of 17 pepper inbred lines which were selected independently. The results showed that chlorophyll contents significantly varied among different inbred lines. To be specific, chlorophyll content of No. 68 inbred line was significantly higher than that of other 14 inbred lines ; chlorophyll content of No. 55 inbred line was signifieandy lower than that of other 15 inbred lines. The chlorophyll content of various inbred lines showed a decreasing order of No. 68 〉 No. 47 〉 No. 66 〉 No. 62 〉 No. 63 〉 No. 60 〉 No. 61 〉 No. 64 〉 No. 56 〉 No. 67 〉 No. 65 〉 No. 48 〉 No. 59 〉 No. 58 〉 No. 57 〉 No. 69 〉 No. 55 ; the photosynthetic rate of various inbred lines showed a decreasing order of No. 68 〉 No. 57 〉 No. 60 〉 No. 48 〉 No. 58 〉 No. 47 〉 No. 62 〉 No. 63 〉 No. 64 〉 No. 65 ; the maximum photesynthetie rate of No. 65 inbred line was significantly lower than other seven inbred lines, while no signifi- cant difference was observed among other nine inbred lines.
文摘The aim of the present study was to investigate the effect of electromagnetic radio frequency treatment of water on the growth of pepper (Capsicum annuum) plants. For this experiment, one hundred one-week old plants were divided into two groups. The first group of plants was watered with water subjected to radio frequency electromagnetic radiation from an internet router for one hour a day, while the other group was watered with tap water (control). The overall results showed changes of growth characters of plant watered with electromagnetic water. The length of pepper plants is significantly affected by the treated water, where the length of shoot was lower in plants grown under the effect of treated water (22.43 ± 7.17 cm) than those grown without treated water (28.11 ± 8.57 cm). The results revealed that the stem diameter of control plants (1.74 ± 0.39 cm) was significantly higher than that of the treated plants (1.66 ± 0.35 cm). In addition, the root length was lower in plants grown under the effect of treated water than those grown without treated water. Pepper plants watered with electromagnetic treated water exhibited marked decreases in health index, fresh and dry weight, relative water content, number of flowers and fruits/plant as well as number of seeds/fruit. In addition, the current experiment showed a significant decrease in the number of leaves, branch and flower per plant when watered with electromagnetic treated water. The results revealed that the first flowering time for plants in treated group was remarkably decelerated when compared to other plants in control group.
基金funded in part by the National Natural Science Foundation of China under Grant No.61872038in part by the Fundamental Research Funds for the Central Universities under Grant No.FRF-GF-20-15B.
文摘Effective user authentication is key to ensuring equipment security,data privacy,and personalized services in Internet of Things(IoT)systems.However,conventional mode-based authentication methods(e.g.,passwords and smart cards)may be vulnerable to a broad range of attacks(e.g.,eavesdropping and side-channel attacks).Hence,there have been attempts to design biometric-based authentication solutions,which rely on physiological and behavioral characteristics.Behavioral characteristics need continuous monitoring and specific environmental settings,which can be challenging to implement in practice.However,we can also leverage Artificial Intelligence(AI)in the extraction and classification of physiological characteristics from IoT devices processing to facilitate authentication.Thus,we review the literature on the use of AI in physiological characteristics recognition pub-lished after 2015.We use the three-layer architecture of the IoT(i.e.,sensing layer,feature layer,and algorithm layer)to guide the discussion of existing approaches and their limitations.We also identify a number of future research opportunities,which will hopefully guide the design of next generation solutions.
基金Supported by National System of Modern Agriculture Industrial Technology(CARS-01-37)Rice Innovation Team of Liaoning Province of National System of Modern Agriculture Industrial Technology([2013]271)Key Agriculture and Achievements Specialization Planning Program of Liaoning Province(2014020004-101-3)
文摘The research performed analysis on differences of physiological property and yield of Tiejing No. 11 by different nitrogen fertilization patterns. The results indicated that while the nitrogen of basal: tillering: panicle was 6 ∶3∶1, the higher amount of nitrogen improved the chlorophyll content, photosynthetic capacity, effective panicle, grain weight and yield; the medium nitrogen level had advantage under the ratio 4:3:3; nitrogen application at lategrowth stage was suitable for low or medium nitrogen level,which could increase photosynthetic capacity and optimize the yield components and increase yield; under the same ratio for basic tiller and ear fertilizer, the increase of nitrogen could improve total nitrogen uptake and protein content, decrease nitrogen physiological efficiency and milled rice and tasting, but nitrogen recovery and harvest index changes had difference; as application at late growth stage in same nitrogen rate, total nitrogen uptake and protein content increased, nitrogen physiological efficiency and milled rice and amylose content and tasting decreased, nitrogen recovery and harvest index increased under low or medium nitrogen level and decreased under high nitrogen level. Nitrogen application 210 kg/hm^2 and the ratio 4 ∶3 ∶3 of basal:tillering: panicle made the yield increase by 2.8%-11.3%, and this is the optimal mode of nitrogen fertilizer application.