Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP ...Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP and EP and the bioavailability of CP in human studies,particularly in young and middle-aged women,remain underexplored.Objective:To evaluate the effects of an orally administered collagen drink combining CP and EP on the skin health of young and middle-aged women.Materials and Methods:A single-center,randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLABR fish collagen tripeptide beverage.Participants consumed the drink over an 8-week period.Results:Compared to the placebo group,the collagen drink group showed significant improvements in skin hydration(39.19%increase),transepidermal water loss(33.45%decrease),skin elasticity(25.37%increase),dermal collagen content(21.64%increase),pore size(7.94%decrease),wrinkle length(18.09%decrease),skin smoothness(2.85%improvement),and skin roughness(15.32%decrease).Overall pore volume decreased by 60%,and visual assessments indicated a decrease in skin luminosity by 15.20%and smoothness index by 22.55%.Mass spectrometry demonstrated a significant increase in collagen efficacy components,including blood pH and GPH levels(P<0.05).Conclusion:The study confirmed the combination nourishing and anti-skin aging effects of EP and CP on the skin of young and middle-aged women,demonstrating significant improvements in various skin parameters and good bioavailability of collagen peptides.展开更多
为了解决传统栖息地预测模型中无法捕捉具有时间序列信息的环境因子对金枪鱼空间分布滞后影响的不足。采用2021—2024年金枪鱼围网渔捞日志数据,通过构建滞后天数为1、5、10、15 d的长短期记忆(Long-short term memory,LSTM)神经网络模...为了解决传统栖息地预测模型中无法捕捉具有时间序列信息的环境因子对金枪鱼空间分布滞后影响的不足。采用2021—2024年金枪鱼围网渔捞日志数据,通过构建滞后天数为1、5、10、15 d的长短期记忆(Long-short term memory,LSTM)神经网络模型,分别对单位捕捞努力量渔获量(Catch per unit of effort,CPUE)和经纬度进行了预测。研究表明,滞后10 d的模型精度最高,其均方误差(Mean square error,MSE)为0.018 7,平均绝对误差(Mean absolute error,MAE)为0.077 6,表明鲣空间分布受过去短期内环境累计效应的影响。通过对最佳模型进行验证,结果表明预测纬度与实际纬度之间的R2为0.97,预测经度与实际经度之间的R2为0.65,说明空间分布预测范围与实际基本吻合。为揭示鲣栖息地特征及其生态过程的动态机制提供了新的理解,同时为中西太平洋鲣围网渔业的科学管理提供了重要参考依据。展开更多
为提高弹性蛋白肽(elastin peptide,EP)的理化性质与功能特性,该研究通过酶解联合微生物(副干酪乳杆菌YYS-K1)发酵制备弹性蛋白肽(elastin peptide by enzymolysis combined with microbial fermentation,EMEP),以单一蛋白酶解制备EP为...为提高弹性蛋白肽(elastin peptide,EP)的理化性质与功能特性,该研究通过酶解联合微生物(副干酪乳杆菌YYS-K1)发酵制备弹性蛋白肽(elastin peptide by enzymolysis combined with microbial fermentation,EMEP),以单一蛋白酶解制备EP为对照,比较EMEP和EP的理化性质和功能特性的差异。结果表明:EMEP的糖胺聚糖的含量较EP提高了约1.73倍;两种EP中氨基酸组成和必需氨基酸占比无明显差异,而EMEP中的Gly和Leu含量占比明显高于EP;分子质量分布结果表明,EMEP中<1000 Da肽段占比为89.37%,单一酶解制备的EP占比为89.19%。抗氧化结果表明,两种EP均具有一定的抗氧化能力,在质量浓度为40 mg/mL时,EMEP的DPPH自由基、ABTS阳离子自由基和超氧阴离子自由基清除率较EP分别提高29.59%、2.74%和8.45%;EP与EMEP均表现出良好的功能特性,其中质量浓度为40 mg/mL的EMEP可有效抑制白蛋白变性、AGEs生成以及胰脂肪酶和弹性蛋白酶的活性,抑制率分别达到了92.11%、70.14%、81.14%和83.33%,与单一蛋白酶解EP相比分别提高20.25%、12.66%、81.14%和20.85%。总之,酶解联合微生物发酵制备法有利于进一步提高EP的理化性质和功能特性,为开发高质量的弹性蛋白肽产品提供理论依据。展开更多
Background:The efficacy of balloon angioplasty for treating peripheral artery disease is influenced by various factors,some of them not yet totally understood.This study aimed to evaluate the role of elastin content i...Background:The efficacy of balloon angioplasty for treating peripheral artery disease is influenced by various factors,some of them not yet totally understood.This study aimed to evaluate the role of elastin content in vascular responses 28 days postangioplasty using uncoated and paclitaxel-coated balloons with the same platform in femoral arteries of a healthy porcine model.Methods:Eight animals underwent balloon angioplasty on the external and internal branches of femoral arteries.Histopathologic evaluation was conducted at follow-up to assess the elastin content,vascular damage,morphological features,and neointimal formation.Results:The elastin content was significantly higher in the external than in the internal femoral artery(p=0.0014).After balloon angioplasty,it was inversely correlated with vascular injury score(ρ=−0.4510,p=0.0096),neointimal inflammation(ρ=−0.3352,p=0.0607),transmural(ρ=−0.4474,p=0.0103)and circumferential(ρ=−0.4591,p=0.0082)smooth muscle cell loss,presence of proteoglycans(ρ=−0.5172,p=0.0024),fibrin deposition(ρ=−0.3496,p=0.0499),and adventitial fibrosis(ρ=−0.6229,p=0.0002).Neointimal formation inhibition with paclitaxel was evident only in arteries with disruption of the internal elastic lamina,with a significant smaller neointimal area in arteries treated with paclitaxel-coated balloons compared to uncoated balloons(median[Q1–Q3]:10.25[7.49–15.64]vs.24.44[18.96–30.52],p=0.0434).Conclusions:Elastin content varies between branches of the femoral artery and significantly influences the integrity of the internal elastic lamina,the vessel's adaptive response,and paclitaxel efficacy after balloon angioplasty.展开更多
文摘Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP and EP and the bioavailability of CP in human studies,particularly in young and middle-aged women,remain underexplored.Objective:To evaluate the effects of an orally administered collagen drink combining CP and EP on the skin health of young and middle-aged women.Materials and Methods:A single-center,randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLABR fish collagen tripeptide beverage.Participants consumed the drink over an 8-week period.Results:Compared to the placebo group,the collagen drink group showed significant improvements in skin hydration(39.19%increase),transepidermal water loss(33.45%decrease),skin elasticity(25.37%increase),dermal collagen content(21.64%increase),pore size(7.94%decrease),wrinkle length(18.09%decrease),skin smoothness(2.85%improvement),and skin roughness(15.32%decrease).Overall pore volume decreased by 60%,and visual assessments indicated a decrease in skin luminosity by 15.20%and smoothness index by 22.55%.Mass spectrometry demonstrated a significant increase in collagen efficacy components,including blood pH and GPH levels(P<0.05).Conclusion:The study confirmed the combination nourishing and anti-skin aging effects of EP and CP on the skin of young and middle-aged women,demonstrating significant improvements in various skin parameters and good bioavailability of collagen peptides.
文摘为了解决传统栖息地预测模型中无法捕捉具有时间序列信息的环境因子对金枪鱼空间分布滞后影响的不足。采用2021—2024年金枪鱼围网渔捞日志数据,通过构建滞后天数为1、5、10、15 d的长短期记忆(Long-short term memory,LSTM)神经网络模型,分别对单位捕捞努力量渔获量(Catch per unit of effort,CPUE)和经纬度进行了预测。研究表明,滞后10 d的模型精度最高,其均方误差(Mean square error,MSE)为0.018 7,平均绝对误差(Mean absolute error,MAE)为0.077 6,表明鲣空间分布受过去短期内环境累计效应的影响。通过对最佳模型进行验证,结果表明预测纬度与实际纬度之间的R2为0.97,预测经度与实际经度之间的R2为0.65,说明空间分布预测范围与实际基本吻合。为揭示鲣栖息地特征及其生态过程的动态机制提供了新的理解,同时为中西太平洋鲣围网渔业的科学管理提供了重要参考依据。
基金iVascular,S.L.U.,Camíde Can Ubach,11–Nave 3,08620 Sant Vicençdels Horts,Barcelona,Spain。
文摘Background:The efficacy of balloon angioplasty for treating peripheral artery disease is influenced by various factors,some of them not yet totally understood.This study aimed to evaluate the role of elastin content in vascular responses 28 days postangioplasty using uncoated and paclitaxel-coated balloons with the same platform in femoral arteries of a healthy porcine model.Methods:Eight animals underwent balloon angioplasty on the external and internal branches of femoral arteries.Histopathologic evaluation was conducted at follow-up to assess the elastin content,vascular damage,morphological features,and neointimal formation.Results:The elastin content was significantly higher in the external than in the internal femoral artery(p=0.0014).After balloon angioplasty,it was inversely correlated with vascular injury score(ρ=−0.4510,p=0.0096),neointimal inflammation(ρ=−0.3352,p=0.0607),transmural(ρ=−0.4474,p=0.0103)and circumferential(ρ=−0.4591,p=0.0082)smooth muscle cell loss,presence of proteoglycans(ρ=−0.5172,p=0.0024),fibrin deposition(ρ=−0.3496,p=0.0499),and adventitial fibrosis(ρ=−0.6229,p=0.0002).Neointimal formation inhibition with paclitaxel was evident only in arteries with disruption of the internal elastic lamina,with a significant smaller neointimal area in arteries treated with paclitaxel-coated balloons compared to uncoated balloons(median[Q1–Q3]:10.25[7.49–15.64]vs.24.44[18.96–30.52],p=0.0434).Conclusions:Elastin content varies between branches of the femoral artery and significantly influences the integrity of the internal elastic lamina,the vessel's adaptive response,and paclitaxel efficacy after balloon angioplasty.
