For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1....For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.展开更多
Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-e...Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-ever,whether the faecal microbiota serves as a surrogate to reflect the dynamics of the gut microbiota has not been determined.Methods:Using 16 S rRNA sequencing technology,the present study investigated the microbiota in three seg-ments(stomach,colon and caecum)of mice to comprehensively define microbial dysregulation and regulation related to rheumatoid arthritis(RA).Results:The results showed that the composition and distribution of the microbiota in the stomach,colon and caecum were significantly different depending on their intrinsic environment and their functions,and the colon possessed the richest and most diverse microbiota structure.The gut microbiota of AIA mice showed ecological dysbiosis,and a dietary intervention with skipjack tuna elastin peptides improved this negative effect,partic-ularly the changes in the gastric microbiota,which were most obvious at RA onset and remission.Implication:The results not only indicate the spatial heterogeneity of the gut microbiota in terms of composition and distribution,but also indicate the potential of the application of elastin peptides for the alleviation of RA.展开更多
针对波浪能发电量取决于当地波浪特征和波浪能发电装置,而传统单一的波能流密度评估法则只考虑了地区波浪特性,未结合装置加以分析,评估结果偏向理论的问题,本文利用欧洲中期天气预报中心(European Centre for Medium-Range Weather For...针对波浪能发电量取决于当地波浪特征和波浪能发电装置,而传统单一的波能流密度评估法则只考虑了地区波浪特性,未结合装置加以分析,评估结果偏向理论的问题,本文利用欧洲中期天气预报中心(European Centre for Medium-Range Weather Forecasts, ECMWF)提供的第五代大气再分析数据集(ECMWF Reanalysis v5, ERA5)中的再分析波浪数据,通过装置得分的波浪能评估方法对北太平洋区域1979—2020年波浪能的空间分布进行了分析。结果表明:在本研究所涉及的地区中,纬度较高的地区波能流密度大且装置得分高,本研究所用评估方法的结论同传统评估方法一致,但在中国南海和部分太平洋纬度较低的地区,发电装置有着较好的连续发电条件,发电效率较高。不同装置间的适配区域也有很大不同,AquaBuoy和Pelamis装置的最佳适用区位于北太平洋纬度较高的地区,而Wave Dragon装置的最佳适用区范围更广,在150°E—160°W之间的纬度较低的地区也表现突出。对于中国南海海域,AquaBuoy装置的适配性最好,而北太平洋纬度较高的地区则最适合布置Pelamis装置,北太平洋纬度中游和较低区域最适合布置Wave Dragon装置。展开更多
The purpose of this paper is to determine the optimal size and number of tubes for a generic attenuator that is similar to Pelamis P2, the wave energy converter. Simulations using ANSYS Workbench, Design Modeler, and ...The purpose of this paper is to determine the optimal size and number of tubes for a generic attenuator that is similar to Pelamis P2, the wave energy converter. Simulations using ANSYS Workbench, Design Modeler, and AQWA are performed to study the energy absorption at the nodes between the tubes. The analysis is limited to linearized hydrodynamic fluid waves loading on floating bodies by employing three-dimensional radiation/diffraction theory in regular waves in the frequency domain. Three sets of tests are conducted by varying total tube number, each tube length and the order of tubes with different lengths. After a systematic study in the frequency domain, the optimal size and number of the genetic attenuator is recommended.展开更多
基金funded by the National Natural Science Foundation of China(No.82073764)the Ten-thousand Talents Plan of Zhejiang Province(No.2019R52026).
文摘For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.
基金sponsored by the National Key R&D Program of China(2018YFD0901102)the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(ZS20190105)+2 种基金the Natural Foundation of Zhejiang Province(LQ22D060002)the Cooperative Project of Vigour Health Products Co.,Ltd,Shenzhenthe K.C.Wong Magna Fund of Ningbo University.
文摘Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-ever,whether the faecal microbiota serves as a surrogate to reflect the dynamics of the gut microbiota has not been determined.Methods:Using 16 S rRNA sequencing technology,the present study investigated the microbiota in three seg-ments(stomach,colon and caecum)of mice to comprehensively define microbial dysregulation and regulation related to rheumatoid arthritis(RA).Results:The results showed that the composition and distribution of the microbiota in the stomach,colon and caecum were significantly different depending on their intrinsic environment and their functions,and the colon possessed the richest and most diverse microbiota structure.The gut microbiota of AIA mice showed ecological dysbiosis,and a dietary intervention with skipjack tuna elastin peptides improved this negative effect,partic-ularly the changes in the gastric microbiota,which were most obvious at RA onset and remission.Implication:The results not only indicate the spatial heterogeneity of the gut microbiota in terms of composition and distribution,but also indicate the potential of the application of elastin peptides for the alleviation of RA.
文摘The purpose of this paper is to determine the optimal size and number of tubes for a generic attenuator that is similar to Pelamis P2, the wave energy converter. Simulations using ANSYS Workbench, Design Modeler, and AQWA are performed to study the energy absorption at the nodes between the tubes. The analysis is limited to linearized hydrodynamic fluid waves loading on floating bodies by employing three-dimensional radiation/diffraction theory in regular waves in the frequency domain. Three sets of tests are conducted by varying total tube number, each tube length and the order of tubes with different lengths. After a systematic study in the frequency domain, the optimal size and number of the genetic attenuator is recommended.
