Background:Neuro-inflammation is regarded as one of the critical pathogenesis in neurodegenerative diseases,which is characterized by the activated microglial cells.Pectolinarin(Pec),a natural flavonoid that exists in...Background:Neuro-inflammation is regarded as one of the critical pathogenesis in neurodegenerative diseases,which is characterized by the activated microglial cells.Pectolinarin(Pec),a natural flavonoid that exists in many Chinese herbal medicines,has been reported to have various biological activities.However,the effects and mechanisms on neuro-inflammation are not clear.Methods:In this study,the inhibitory effects and mechanisms of Pec on neuro-inflammation were investigated in the LPS-stimulated microglial BV_(2) cells.BV_(2) microglial cells were treated with Pec or vehicle,followed by LPS.Enzyme-linked immunosorbent assay,real-time quantitative PCR,nitric oxide and reactive oxygen species assay,and western blot were performed to examine the effects of Pec on neuro-inflammatory responses.Results:We showed that Pec significantly inhibited the expression of tumor necrosis factorαand interleukin 6 in mRNA and protein levels induced by LPS.Moreover,the production of nitric oxide,iNOS,reactive oxygen species,and COX-2 were suppressed by Pec in LPS-stimulated microglial BV_(2) cells.In addition,Pec inhibited LPS-induced inflammation via nuclear factor kappa B signaling pathway,as evidenced by the reduction of the phosphorylation of inhibitor of nuclear factor kappa-B kinase,the degradation of IκBα,and the nuclear translocation of p65.Conclusion:Taken together,Pec exhibited anti-inflammatory effects in LPS-stimulated microglial BV_(2) cells via nuclear factor kappa B signaling pathway,which might provide therapeutic potential for neuro-inflammation and neurodegenerative diseases.展开更多
Pectolinarin,a flavonoid isolated from Cirsium species,which has been proved to have the function of food-borne adjuvant therapy in cancer.The reduced expression of PGC1αin various cancers is correlated with poor pat...Pectolinarin,a flavonoid isolated from Cirsium species,which has been proved to have the function of food-borne adjuvant therapy in cancer.The reduced expression of PGC1αin various cancers is correlated with poor patient prognosis.This study investigates how pectolinarin regulates medullary thyroid cancer(MTC)by targeting PGC1α.Initially,bioinformatics and molecular biology confirmed that PGC1αinhibits the energy metabolism and proliferation of MTC cells through the ANKRD9/LDHA signaling pathway.Virtual screening and molecular docking further identified that pectolinarin binds PGC1αwith high affinity,forming key hydrogen bonds with residues such as THR721,GLU666,and TYR717.This binding interaction suggests that pectolinarin inhibits MTC proliferation by promoting the expression of PGC1α.Pectolinarin modulates the energy metabolism of MTC cells via the PGC1α/ANKRD9/LDHA signaling axis.To further validate these findings,in vivo and patient-derived organoid models confirmed that pectolinarin is an effective natural compound capable of inhibiting the growth of MTC cancer.These findings establish pectolinarin as a promising natural compound for MTC treatment by modulating energy metabolism through the PGC1α/ANKRD9/LDHA axis.展开更多
The consumption of Cirsium japonicum leaves is limited by their short seasonal availability and strong bitterness.This study evaluated the effects of different pretreatment processes on their functional and sensory pr...The consumption of Cirsium japonicum leaves is limited by their short seasonal availability and strong bitterness.This study evaluated the effects of different pretreatment processes on their functional and sensory properties to enhance utilization and commercialization.Thistle leaves were steeped in water,1%salt solution,or 0.1% sodium bicarbonate solution,followed by blanching or steaming,and dried using either hot-air or freeze-drying.Samples steeped in 0.1% sodium bicarbonate solution had the highest L^(*) value(60.70)and rehydration rate(809.33%),with the highest overall acceptability and least bitterness in sensory analysis when blanched and freeze-dried.The same steeping solution,combined with steaming and freeze-drying,yielded the highest total polyphenol(13.40 mg GAE/g),flavonoid(29.49μg QE/g),and pectolinarin(13.86 mg/100 g)contents,along with the strongest DPPH radical scavenging activity(4.75 mg GAE/g)andα-glucosidase inhibition(74.35%).Meanwhile,blanching followed by hot-air drying resulted in the highest vitamin E content(74.06 mg/100 g).For samples steeped in water,blanching and freeze-drying produced the highestβ-carotene content(46.00 mg/100 g),while steaming and hot-air drying led to the highest vitamin B2 content(1.57 mg/100 g),with the strongest xanthine oxidase inhibition observed in steamed and freeze-dried samples.In contrast,samples steeped in 1%salt solution exhibited the highest folate content when steamed and freeze-dried.Overall,steeping in 0.1%sodium bicarbonate solution,particularly when combined with steaming and freeze-drying,enhanced the functional and sensory properties of dried thistle leaves,making it the most effective pretreatment method.展开更多
基金supported by the grants from the Training Program for Young Backbone Teachers in Colleges and Universities of Henan Province(2021GGJS103)the Program for Science and Technology Innovation Team in Universities of Henan Province(22IRTSTHN030)the Henan Neural Development Engineering Research Center for Children Foundation(SG201909).
文摘Background:Neuro-inflammation is regarded as one of the critical pathogenesis in neurodegenerative diseases,which is characterized by the activated microglial cells.Pectolinarin(Pec),a natural flavonoid that exists in many Chinese herbal medicines,has been reported to have various biological activities.However,the effects and mechanisms on neuro-inflammation are not clear.Methods:In this study,the inhibitory effects and mechanisms of Pec on neuro-inflammation were investigated in the LPS-stimulated microglial BV_(2) cells.BV_(2) microglial cells were treated with Pec or vehicle,followed by LPS.Enzyme-linked immunosorbent assay,real-time quantitative PCR,nitric oxide and reactive oxygen species assay,and western blot were performed to examine the effects of Pec on neuro-inflammatory responses.Results:We showed that Pec significantly inhibited the expression of tumor necrosis factorαand interleukin 6 in mRNA and protein levels induced by LPS.Moreover,the production of nitric oxide,iNOS,reactive oxygen species,and COX-2 were suppressed by Pec in LPS-stimulated microglial BV_(2) cells.In addition,Pec inhibited LPS-induced inflammation via nuclear factor kappa B signaling pathway,as evidenced by the reduction of the phosphorylation of inhibitor of nuclear factor kappa-B kinase,the degradation of IκBα,and the nuclear translocation of p65.Conclusion:Taken together,Pec exhibited anti-inflammatory effects in LPS-stimulated microglial BV_(2) cells via nuclear factor kappa B signaling pathway,which might provide therapeutic potential for neuro-inflammation and neurodegenerative diseases.
基金supported by The National Natural Science Foundation of China(82302965)Henan Medical Science and Technology Key Program(SBGJ202303019,SBGJ202402024).
文摘Pectolinarin,a flavonoid isolated from Cirsium species,which has been proved to have the function of food-borne adjuvant therapy in cancer.The reduced expression of PGC1αin various cancers is correlated with poor patient prognosis.This study investigates how pectolinarin regulates medullary thyroid cancer(MTC)by targeting PGC1α.Initially,bioinformatics and molecular biology confirmed that PGC1αinhibits the energy metabolism and proliferation of MTC cells through the ANKRD9/LDHA signaling pathway.Virtual screening and molecular docking further identified that pectolinarin binds PGC1αwith high affinity,forming key hydrogen bonds with residues such as THR721,GLU666,and TYR717.This binding interaction suggests that pectolinarin inhibits MTC proliferation by promoting the expression of PGC1α.Pectolinarin modulates the energy metabolism of MTC cells via the PGC1α/ANKRD9/LDHA signaling axis.To further validate these findings,in vivo and patient-derived organoid models confirmed that pectolinarin is an effective natural compound capable of inhibiting the growth of MTC cancer.These findings establish pectolinarin as a promising natural compound for MTC treatment by modulating energy metabolism through the PGC1α/ANKRD9/LDHA axis.
文摘The consumption of Cirsium japonicum leaves is limited by their short seasonal availability and strong bitterness.This study evaluated the effects of different pretreatment processes on their functional and sensory properties to enhance utilization and commercialization.Thistle leaves were steeped in water,1%salt solution,or 0.1% sodium bicarbonate solution,followed by blanching or steaming,and dried using either hot-air or freeze-drying.Samples steeped in 0.1% sodium bicarbonate solution had the highest L^(*) value(60.70)and rehydration rate(809.33%),with the highest overall acceptability and least bitterness in sensory analysis when blanched and freeze-dried.The same steeping solution,combined with steaming and freeze-drying,yielded the highest total polyphenol(13.40 mg GAE/g),flavonoid(29.49μg QE/g),and pectolinarin(13.86 mg/100 g)contents,along with the strongest DPPH radical scavenging activity(4.75 mg GAE/g)andα-glucosidase inhibition(74.35%).Meanwhile,blanching followed by hot-air drying resulted in the highest vitamin E content(74.06 mg/100 g).For samples steeped in water,blanching and freeze-drying produced the highestβ-carotene content(46.00 mg/100 g),while steaming and hot-air drying led to the highest vitamin B2 content(1.57 mg/100 g),with the strongest xanthine oxidase inhibition observed in steamed and freeze-dried samples.In contrast,samples steeped in 1%salt solution exhibited the highest folate content when steamed and freeze-dried.Overall,steeping in 0.1%sodium bicarbonate solution,particularly when combined with steaming and freeze-drying,enhanced the functional and sensory properties of dried thistle leaves,making it the most effective pretreatment method.
