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细极链格孢菌peaT2基因在毕赤酵母中的表达及蛋白功能确定 被引量:1
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作者 刘文平 曾洪梅 +2 位作者 刘延锋 袁京京 邱德文 《微生物学报》 CAS CSCD 北大核心 2007年第4期593-597,共5页
从细极链格孢菌表达文库获得阳性克隆子,序列分析表明,克隆的DNA片段中含有完整的开放阅读框架,将该基因命名为peaT2(GenBank登录号为EF212880)。用PCR法扩增peaT2基因的编码序列并亚克隆到毕赤酵母表达系统的表达载体pPIC9K上,得到重... 从细极链格孢菌表达文库获得阳性克隆子,序列分析表明,克隆的DNA片段中含有完整的开放阅读框架,将该基因命名为peaT2(GenBank登录号为EF212880)。用PCR法扩增peaT2基因的编码序列并亚克隆到毕赤酵母表达系统的表达载体pPIC9K上,得到重组质粒pPIC9K/peaT2。重组质粒经SacⅠ线性化后用电穿孔法导入到毕赤酵母(Pichia pastoris)GS115中,采用MD、G418-YPD平板和PCR法筛选Mut+表型,获得了分泌表达的重组毕赤酵母。随机挑取一菌株作为表达菌,用甲醇诱导PeaT2蛋白表达。SDS-PAGE及Western blot检测结果均表明PeaT2在毕赤酵母中成功地分泌表达。用peaT2基因的表达蛋白处理小麦种子,生物测定表明,表达蛋白能明显促进小麦的生长,具有蛋白激发子作用。 展开更多
关键词 peat2 蛋白激发子 细极链格孢菌 毕赤酵母表达系统
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Temperature Effect Investigation toward Peat Surface CO2 Emissions by Planting Leguminous Cover Crops in Oil Palm Plantations in West Kalimantan
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作者 Arifin Suntoro Wongso Atmojo +1 位作者 Prabang Setyono Widyatmani Sih Dewi 《Journal of Agricultural Science and Technology(B)》 2015年第3期170-183,共14页
The aim of this research was to know the impact of planting leguminous cover crops (LCCs) of Mucuna bracteata and Calopogonium mucunoides in oil palm plantation on peatland on reducing CO2 emissions. Atmosphere temp... The aim of this research was to know the impact of planting leguminous cover crops (LCCs) of Mucuna bracteata and Calopogonium mucunoides in oil palm plantation on peatland on reducing CO2 emissions. Atmosphere temperature, peat surface temperature, in-closed chamber temperature and peat surface CO2 fluxes were monitored on two adjacent experimental plots. The first experimental plot was on the newly opened peat surface (NOPS) and another was on the eight years planted oil palm land (EPOL). The closed chamber techniques adopted from International Atomic Energy Agency (IAEA) (1993) were implemented to trap CO2 emissions emitted from 24 treatment plots at the 1st, 3rd and 6th months observations. Average CO2 fluxes observed on no LCCs plots in the NOPS site were 61.25 ± 8.98, 33.76 ± 2.92 and 33.75 ± 3.45 g/m2.h, while in the EPOL site were 55.38 ± 15.95, 29.90 ± 5.32 and 27.70 ± 4.62 g/mLh at the 1st, 3rd and 6th months monitoring, respectively. Average CO2 fluxes observed on the planted M. bracteata plots in the NOPS site were 68.2 ± 24.5, 12.88 ± 3.70 and 10.40 ± 1.28 g/m2.h, whereas in the EPOL site were 54.04 ± 6.70, 11.45 ± 2.00 and 9.33 ± 3.49 g/m2.h at the 1st, 3rd and 6th months monitoring, respectively. Average CO2 flux observed on the planted C. mucunoides plots in the NOPS site were 66.5 ± 23.7, 15.41 ± 1.51 and 9.74 ± 2.55 g/m2.h, while in the EPOL site were 47.00 ± 5.00, 9.34 ± 1.23 and 10.52 ± 4.80 g/m2.h at the 1st, 3rd and 6th months, respectively. P-value for the experimental sites was 0.008 (〈 0.05), indicating the significant difference in the level of CO2 fluxes between the sites. P-value for the treatments in the experimental plots was 0.000 (〈 0.05), indicating markedly different level of CO2 fluxes among treatments. P-value for the age ofM. bracteata and C. mucunoides planted on the experimental plots was 0.000 (〈 0.05), indicating the significant difference in the level of CO2 fluxes due to the enhanced LCCs age performing at the increase of shading effects. The comparison of CO2 fluxes among experimental plots shows that planting M. bracteata and C. mucunoides on the peatland could reduce CO2 emission. 展开更多
关键词 Temperature peat surface CO2 emissions Mucuna bracteata Calopogonium mucunoides.
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