With the increasing accumulation of plastic pollutants in various environments,research on microorganisms(including bacteria,fungi,and algae)with plastic degradation capabilities has gained significant attention.Howev...With the increasing accumulation of plastic pollutants in various environments,research on microorganisms(including bacteria,fungi,and algae)with plastic degradation capabilities has gained significant attention.However,only a limited number of microbial plastic-degrading enzymes have been identified to date.This highlights that the degradation mechanisms employed by many plastic-degrading microorganisms,particularly filamentous fungi,remain insufficiently explored.In this study,we utilized a versatile fungal plasmid(pCT74)to express green fluorescent protein(GFP)in a marine-derived fungus Alternaria alternata strain FB1 with plastic degradation capabilities.Upon evaluating the degradation effect of polyester-type polyurethane(PU)film,we observed that different transformants exhibited three kinds of activities(the same,reduced,or enhanced degradation capability)compared to the FB1 wild-type strain.Further analysis of the plasmid fragment insertion sites in different transformants revealed that pCT74 integrates randomly into the genome of the host fungus.Notably,a direct correlation was found between the plasmid insertion site and the degradation capability of the corresponding transformant.Our findings not only redefine the potential applications of plasmid pCT74 in filamentous fungi but also show a novel research approach to identifying key enzymes involved in plastic degradation by fungi.展开更多
基金Supported by the Science and Technology Innovation Project of Laoshan Laboratory(Nos.2022QNLM030004-3,LSKJ202203103)the NSFC Innovative Group Grant(No.42221005)+5 种基金the Key Collaborative Research Program of the Alliance of International Science Organizations(No.ANSO-CR-KP-2022-08)the Shandong Provincial Natural Science Foundation(No.ZR2021ZD28)the Major Research Plan of the National Natural Science Foundation(No.92351301)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA22050301)the Taishan Scholars Program(No.tstp20230637)the Qingdao Natural Science Foundation(No.23-2-1-182-zyyd-jch)。
文摘With the increasing accumulation of plastic pollutants in various environments,research on microorganisms(including bacteria,fungi,and algae)with plastic degradation capabilities has gained significant attention.However,only a limited number of microbial plastic-degrading enzymes have been identified to date.This highlights that the degradation mechanisms employed by many plastic-degrading microorganisms,particularly filamentous fungi,remain insufficiently explored.In this study,we utilized a versatile fungal plasmid(pCT74)to express green fluorescent protein(GFP)in a marine-derived fungus Alternaria alternata strain FB1 with plastic degradation capabilities.Upon evaluating the degradation effect of polyester-type polyurethane(PU)film,we observed that different transformants exhibited three kinds of activities(the same,reduced,or enhanced degradation capability)compared to the FB1 wild-type strain.Further analysis of the plasmid fragment insertion sites in different transformants revealed that pCT74 integrates randomly into the genome of the host fungus.Notably,a direct correlation was found between the plasmid insertion site and the degradation capability of the corresponding transformant.Our findings not only redefine the potential applications of plasmid pCT74 in filamentous fungi but also show a novel research approach to identifying key enzymes involved in plastic degradation by fungi.
文摘目的探讨白细胞介素-6(interleukin-6,IL-6)和降钙素原(procalcitonin,PCT)水平对急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)患者预后的预测价值。方法选取2021年1月~2023年12月徐州医科大学附属连云港医院收治的99例ARDS患者为研究对象,根据柏林定义将其分为轻度组(n=22)、中度组(n=42)和重度组(n=35),根据其28天转归情况分为生存组(n=65)及死亡组(n=34)。比较各组IL-6、PCT的水平,通过受试者工作特征(receiver operator characteristic,ROC)曲线和曲线下面积(area under the curve,AUC)评估两者在ARDS患者预后中的预测价值。绘制不同IL-6、PCT水平ARDS患者的Kaplan-Meier生存曲线。结果3种不同病情严重程度的ARDS患者中,重度组的IL-6、PCT水平最高,中度组次之,轻度组最低,各组间比较差异均有统计学意义(P<0.05);死亡组患者IL-6、PCT水平明显高于生存组,差异有统计学意义(P<0.05)。IL-6和PCT评价ARDS患者预后的AUC分别为0.793、0.776,敏感度分别为79.4%、94.1%,特异性分别为70.8%、61.5%。Kaplan-Meier生存曲线分析结果显示,IL-6≥110.700pg/ml和PCT≥1.700ng/ml时,ARDS患者28天存活率较IL-6<110.700pg/ml和PCT<1.700ng/ml者显著降低(P<0.001)。结论PCT、IL-6水平对预测ARDS患者的短期预后具有良好价值,可作为评估ARDS患者预后的有效指标。
