Supraparticles(SPs),such as assembly of inorganic components with organic,have made tremendous attention in biochemical analysis,which represents a novel but challenging research orientation.Herein,a single-SPs multif...Supraparticles(SPs),such as assembly of inorganic components with organic,have made tremendous attention in biochemical analysis,which represents a novel but challenging research orientation.Herein,a single-SPs multifunctional fluorescent sensor array has been developed for high-throughput detection of heavy metal ions in biofluids,which is based on an inorganic/organic hybrid SPs consisting of carbon dots(CDs)and an easily available porphyrin[5,10,15,20-tetra(4-carboxyphenyl)porphyrin(TCPP)].TCPP can aggregate with the CDs to form the assembly(CDs/TCPP SPs)through the electrostatic andπ-πstacking interaction.There are two independent and clearly separated fluorescence emission peaks at 470 and 668 nm in the resultant CDs/TCPP SPs under 380 nm excitation.As a proof-of concept design,F 470,F 668,F 668/F 470 of SPs are chosen as three sensor components to constitute our sensor array.With the addi-tion of metal ions,three sensor components can generate different fluorescence response patterns for discriminating 11 heavy metal ions via principal component analysis(PCA).Additionally,thiols can read-ily capture Cu^(2+)to switch the fluorescence of CDs/TCPP initially altered by Cu^(2+).Hence,CDs/TCPP-Cu^(2+)ensemble is further demonstrated to be a powerful sensor array for pattern recognition of 7 thiols and even chiral recognition of cysteine enantiomers.This novel strategy avoids the tanglesome synthesis of multiple sensing probes and dedicates an innovative method for the facile establishment of tongue-mimic sensors,which would prospectively sprout more homologous assumptions to broaden its application toward more biosensing fields.展开更多
Sensor networks tend to support different traffic patterns since more and more emerging applications have diverse needs. We present MGRP, a Multi-Gradient Routing Protocol for wireless sensor networks, which is fully ...Sensor networks tend to support different traffic patterns since more and more emerging applications have diverse needs. We present MGRP, a Multi-Gradient Routing Protocol for wireless sensor networks, which is fully distributed and efficiently supports endto-end, one-to-many and many-to-one traffic patterns by effectively construct and maintain a gradient vector for each node. We further combine neighbor link estimation with routing information to reduce packet exchange on network dynamics and node failures. We have implemented MGRP on Tiny OS and evaluated its performance on real-world testbeds. The result shows MGRP achieves lower end-to-end packet delay in different traffic patterns compared to the state of the art routing protocols while still remains high packet delivery ratio.展开更多
Lanthanide-doped upconversion nanoparticles(Ln-UCNPs)are a new type of nanomaterials with excellent fluorescence properties,which are well applied in fluorescent biosensing.Herein we developed a multifunctional probe ...Lanthanide-doped upconversion nanoparticles(Ln-UCNPs)are a new type of nanomaterials with excellent fluorescence properties,which are well applied in fluorescent biosensing.Herein we developed a multifunctional probe based on the surface engineering of core-shell structure UCNPs with polyacrylic acid(PAA).The developed PAA/UCNPs probe could be highly selective to detect and respond to Cu^(2+) at different pH.Cu^(2+) could easily combine with the carboxylate anion of PAA to quench the fluorescence of UCNPs.Therefore,we creatively proposed a fluorescent array sensor(PAA/UCNPs-Cu^(2+)),in which the same material acted as the sensing element by coupled with pH regulation for pattern recognition of 5 thiols.It could also easily identify the chiral enantiomer of cystine(L-Cys-and D-Cys),and distinguish their mixed samples with different concentrations,and more importantly,it could be combined with urine samples to detect actual level of homocysteine(Hcys)to provide a new solution for judging whether the human body suffers from homocystinuria.展开更多
基金supported by the National Natural Science Foun-dation of China(No.21775044)the Shanghai Science and Tech-nology Committee(Nos.19ZR1473300,18DZ1112700)+1 种基金the Shanghai Key Laboratory of Multidimensional Information Processing(No.MIP202104)the Fundamental Research Funds for the Central Universities.
文摘Supraparticles(SPs),such as assembly of inorganic components with organic,have made tremendous attention in biochemical analysis,which represents a novel but challenging research orientation.Herein,a single-SPs multifunctional fluorescent sensor array has been developed for high-throughput detection of heavy metal ions in biofluids,which is based on an inorganic/organic hybrid SPs consisting of carbon dots(CDs)and an easily available porphyrin[5,10,15,20-tetra(4-carboxyphenyl)porphyrin(TCPP)].TCPP can aggregate with the CDs to form the assembly(CDs/TCPP SPs)through the electrostatic andπ-πstacking interaction.There are two independent and clearly separated fluorescence emission peaks at 470 and 668 nm in the resultant CDs/TCPP SPs under 380 nm excitation.As a proof-of concept design,F 470,F 668,F 668/F 470 of SPs are chosen as three sensor components to constitute our sensor array.With the addi-tion of metal ions,three sensor components can generate different fluorescence response patterns for discriminating 11 heavy metal ions via principal component analysis(PCA).Additionally,thiols can read-ily capture Cu^(2+)to switch the fluorescence of CDs/TCPP initially altered by Cu^(2+).Hence,CDs/TCPP-Cu^(2+)ensemble is further demonstrated to be a powerful sensor array for pattern recognition of 7 thiols and even chiral recognition of cysteine enantiomers.This novel strategy avoids the tanglesome synthesis of multiple sensing probes and dedicates an innovative method for the facile establishment of tongue-mimic sensors,which would prospectively sprout more homologous assumptions to broaden its application toward more biosensing fields.
基金supported by National Key Technologies Research and Development Program of China under Grant No.2014BAH14F01National Science and Technology Major Project of China under Grant No.2012ZX03005007+1 种基金National NSF of China Grant No.61402372Fundamental Research Funds for the Central Universities Grant No.3102014JSJ0003
文摘Sensor networks tend to support different traffic patterns since more and more emerging applications have diverse needs. We present MGRP, a Multi-Gradient Routing Protocol for wireless sensor networks, which is fully distributed and efficiently supports endto-end, one-to-many and many-to-one traffic patterns by effectively construct and maintain a gradient vector for each node. We further combine neighbor link estimation with routing information to reduce packet exchange on network dynamics and node failures. We have implemented MGRP on Tiny OS and evaluated its performance on real-world testbeds. The result shows MGRP achieves lower end-to-end packet delay in different traffic patterns compared to the state of the art routing protocols while still remains high packet delivery ratio.
基金supported by the National Natural Science Foundation of China(No.21775044)the Shanghai Science and Technology Committee(Nos.19ZR1473300 and 18DZ1112700)the Fundamental Research Funds for the Central Universities。
文摘Lanthanide-doped upconversion nanoparticles(Ln-UCNPs)are a new type of nanomaterials with excellent fluorescence properties,which are well applied in fluorescent biosensing.Herein we developed a multifunctional probe based on the surface engineering of core-shell structure UCNPs with polyacrylic acid(PAA).The developed PAA/UCNPs probe could be highly selective to detect and respond to Cu^(2+) at different pH.Cu^(2+) could easily combine with the carboxylate anion of PAA to quench the fluorescence of UCNPs.Therefore,we creatively proposed a fluorescent array sensor(PAA/UCNPs-Cu^(2+)),in which the same material acted as the sensing element by coupled with pH regulation for pattern recognition of 5 thiols.It could also easily identify the chiral enantiomer of cystine(L-Cys-and D-Cys),and distinguish their mixed samples with different concentrations,and more importantly,it could be combined with urine samples to detect actual level of homocysteine(Hcys)to provide a new solution for judging whether the human body suffers from homocystinuria.
