Background:Accumulating data have suggested that long non-coding RNAs(lncRNAs)play important roles in regulating tumor cell growth.This study was designed to investigate the role of SNHG16 in hep-atocellular carcinoma...Background:Accumulating data have suggested that long non-coding RNAs(lncRNAs)play important roles in regulating tumor cell growth.This study was designed to investigate the role of SNHG16 in hep-atocellular carcinoma(HCC).Methods:SNHG16 expression was detected with real-time polymerase chain reaction(PCR).The cut-offvalue of SNHG16 for tumor-free survival(TFS)was determined with receiver operating characteristic curve analysis.Small interfering RNA was used to inhibit the expression of SNHG16 in HCC cell lines.The biologic behavior of HCC cell was determined with cell viability assay and Transwell assay in vitro.The potential predictive value of SNHG16 on prognosis was analyzed by Kaplan-Meier curves and Cox proportional hazards regression model.Results:SNHG16 expression was upregulated in tumor tissues and HCC cell lines.High expression of SNHG16 was associated with tumor recurrence and poor prognosis after surgery.Multivariate analysis revealed that SNHG16 was an independent prognostic factor for poor recurrence-free survival.Moreover,inhibition of SNHG16 in HepG2,Hep3B,and BEL-7402 cells significantly reduced cell invasiveness and proliferation.Mechanistic analyses indicated that the ECM-receptor interaction pathway was remarkably activated by SNHG16.Conclusions:SNHG16 might be a promising biomarker for predicting tumor recurrence in HCC patients after surgery and a potential therapeutic target for HCC.展开更多
Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant ...Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.展开更多
Background:Cryoablation(CRYO)is a novel catheter ablation technique for atrial fibrillation(AF).However,un-certainty persists regarding the role of metabolic modifications associated with CRYO.This study was aimed at ...Background:Cryoablation(CRYO)is a novel catheter ablation technique for atrial fibrillation(AF).However,un-certainty persists regarding the role of metabolic modifications associated with CRYO.This study was aimed at explor-ing whether CRYO influences the metabolic signature–a possibility not previously investigated.Methods:Paired serum samples from patients with AF(n=10)were collected before and 24 h after CRYO.Untar-geted metabolomic analysis was conducted with LC-MS.Univariate and multivariate analyses were applied to identify differential metabolites between samples.Pathway enrichment and Pearson correlation analyses were performed to reveal the perturbed metabolic pathways and potential interactions.Results:Levels of 19 metabolites showed significant changes between baseline and 24 h after CRYO.Pathway analysis revealed that the perturbed metabolites were enriched in unsaturated fatty acid biosynthesis,retrograde en-docannabinoid signaling,and neuroactive ligand-receptor interactions.Pearson correlation analysis indicated strong correlations among differential metabolites,biochemical markers,and clinical indicators.Conclusions:CRYO induces systemic changes in the serum metabolome in patients with paroxysmal AF and pro-vides potential metabolic benefits.Our findings might enable enhanced understanding of the pathophysiology and metabolic mechanisms involved in catheter ablation.展开更多
Cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies....Cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies. The precise determination of the specific composition of protein complexes, especially using scalable and high-throughput methods, represents a systematic approach toward revealing particular cellular biological functions. In this regard, the direct profiling protein-protein interactions (PPIs) represent an efficient way to dissect functional pathways for revealing novel protein functions. In this review, we illustrate the technological evolution for the large-scale and precise identification of PPIs toward higher physiologically relevant accuracy. These techniques aim at improving the efficiency of complex pull-down, the signal specificity and accuracy in distinguishing specific PPIs, and the accuracy of identifying physiological relevant PPIs. A newly developed streamline proteomic approach for mapping the binary relationship of PPIs in a protein complex is introduced.展开更多
A good drug or drug candidate should not only interact with its target molecule effectively and specifically,but also be absorbed into the body,distributed to the right location,metabolized into right compounds,and el...A good drug or drug candidate should not only interact with its target molecule effectively and specifically,but also be absorbed into the body,distributed to the right location,metabolized into right compounds,and eliminated out of the body in proper manner.The processes of drug getting into and out of the body involve Absorption,Distribution,Metabolism,and Excretion（ADME）,展开更多
基金supported by grants from Science and Tech-nology Projects of Medicine and Health in Zhejiang Province(2020383364)Natural Science Foundation of Zhejiang Province(LY21H160055).
文摘Background:Accumulating data have suggested that long non-coding RNAs(lncRNAs)play important roles in regulating tumor cell growth.This study was designed to investigate the role of SNHG16 in hep-atocellular carcinoma(HCC).Methods:SNHG16 expression was detected with real-time polymerase chain reaction(PCR).The cut-offvalue of SNHG16 for tumor-free survival(TFS)was determined with receiver operating characteristic curve analysis.Small interfering RNA was used to inhibit the expression of SNHG16 in HCC cell lines.The biologic behavior of HCC cell was determined with cell viability assay and Transwell assay in vitro.The potential predictive value of SNHG16 on prognosis was analyzed by Kaplan-Meier curves and Cox proportional hazards regression model.Results:SNHG16 expression was upregulated in tumor tissues and HCC cell lines.High expression of SNHG16 was associated with tumor recurrence and poor prognosis after surgery.Multivariate analysis revealed that SNHG16 was an independent prognostic factor for poor recurrence-free survival.Moreover,inhibition of SNHG16 in HepG2,Hep3B,and BEL-7402 cells significantly reduced cell invasiveness and proliferation.Mechanistic analyses indicated that the ECM-receptor interaction pathway was remarkably activated by SNHG16.Conclusions:SNHG16 might be a promising biomarker for predicting tumor recurrence in HCC patients after surgery and a potential therapeutic target for HCC.
基金supported by the National Natural Science Foundations of China (31402033, U1603232)the Special Fund for Basic Scientific Research of Institute of Animal Sciences,the Chinese Academy of Agricultural Sciences (2017ywf-zd-11, Y2017JC03)the Agricultural Science and Technology Innovation Program of China (ASTIPIAS01)
文摘Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.
基金supported by grants from National Natural Science Foundation of China(82241057,82270532,81970287,82100530,and 82200556)the Foundation for Innovative Research Groups of Natural Science Foundation of Hubei Province,China(2021CFA010).
文摘Background:Cryoablation(CRYO)is a novel catheter ablation technique for atrial fibrillation(AF).However,un-certainty persists regarding the role of metabolic modifications associated with CRYO.This study was aimed at explor-ing whether CRYO influences the metabolic signature–a possibility not previously investigated.Methods:Paired serum samples from patients with AF(n=10)were collected before and 24 h after CRYO.Untar-geted metabolomic analysis was conducted with LC-MS.Univariate and multivariate analyses were applied to identify differential metabolites between samples.Pathway enrichment and Pearson correlation analyses were performed to reveal the perturbed metabolic pathways and potential interactions.Results:Levels of 19 metabolites showed significant changes between baseline and 24 h after CRYO.Pathway analysis revealed that the perturbed metabolites were enriched in unsaturated fatty acid biosynthesis,retrograde en-docannabinoid signaling,and neuroactive ligand-receptor interactions.Pearson correlation analysis indicated strong correlations among differential metabolites,biochemical markers,and clinical indicators.Conclusions:CRYO induces systemic changes in the serum metabolome in patients with paroxysmal AF and pro-vides potential metabolic benefits.Our findings might enable enhanced understanding of the pathophysiology and metabolic mechanisms involved in catheter ablation.
基金support from the Shanghai Science and Technology Development Program (Grant Nos. 03DZ14024 & 07ZR14010)the 863 High Technology Foundation of China (Grant No. 2006AA02A310)+1 种基金US NIH 1R01AI064806-01A2, 5R21DK082706U.S. Department of Energy, the Office of Science (BER) (Grant No. DE-FG02- 07ER64422)
文摘Cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies. The precise determination of the specific composition of protein complexes, especially using scalable and high-throughput methods, represents a systematic approach toward revealing particular cellular biological functions. In this regard, the direct profiling protein-protein interactions (PPIs) represent an efficient way to dissect functional pathways for revealing novel protein functions. In this review, we illustrate the technological evolution for the large-scale and precise identification of PPIs toward higher physiologically relevant accuracy. These techniques aim at improving the efficiency of complex pull-down, the signal specificity and accuracy in distinguishing specific PPIs, and the accuracy of identifying physiological relevant PPIs. A newly developed streamline proteomic approach for mapping the binary relationship of PPIs in a protein complex is introduced.
基金supported by the Natural Science Foundation ofChina(Nos.30873159 and 31271405)
文摘A good drug or drug candidate should not only interact with its target molecule effectively and specifically,but also be absorbed into the body,distributed to the right location,metabolized into right compounds,and eliminated out of the body in proper manner.The processes of drug getting into and out of the body involve Absorption,Distribution,Metabolism,and Excretion（ADME）,
