Soybean mosaic virus(SMV;Potyvirus,Potyviridae)is one of the most prevalent and destructive viral pathogens in the world.The P1 protein is the first N-terminal product in the potyvirus genome and shows a high sequence...Soybean mosaic virus(SMV;Potyvirus,Potyviridae)is one of the most prevalent and destructive viral pathogens in the world.The P1 protein is the first N-terminal product in the potyvirus genome and shows a high sequence variability that may be related to virus adaptation to hosts.In this work,we focused on the different functions of P1 proteins in two SMV isolates SMVGZL and SMV^(NB)during their infection of plants.Isolate SMVGZL induced weaker symptoms than SMV^(NB)in mechanical inoculation assays,and the accumulation level of SMV CP in SMVGZL-infected leaves was lower than that in leaves infected with SMV^(NB),especially at the late stage of infection.The isolates SMVGZL and SMV^(NB)had a high similarity in genome sequence except for the P1 region.P1^(GZL)induced a higher salicylic acid(SA)response than P1NB in Nicotiana benthamiana,which may explain the lower virus titers in plants infected with SMV^(GZL).Our results suggest that the divergence in the P1 proteins of these SMV isolates influenced their virulence via differentially regulating SA signaling.展开更多
基金supported by the Ningbo Public Welfare Technology Plan Project(2021S012)Science Foundation of Zhejiang Province(LQ21C140004)+1 种基金the China National Novel Transgenic Organisms Breeding Project(2019ZX08004-004)K.C.Wong Magna Fund in Ningbo University.
文摘Soybean mosaic virus(SMV;Potyvirus,Potyviridae)is one of the most prevalent and destructive viral pathogens in the world.The P1 protein is the first N-terminal product in the potyvirus genome and shows a high sequence variability that may be related to virus adaptation to hosts.In this work,we focused on the different functions of P1 proteins in two SMV isolates SMVGZL and SMV^(NB)during their infection of plants.Isolate SMVGZL induced weaker symptoms than SMV^(NB)in mechanical inoculation assays,and the accumulation level of SMV CP in SMVGZL-infected leaves was lower than that in leaves infected with SMV^(NB),especially at the late stage of infection.The isolates SMVGZL and SMV^(NB)had a high similarity in genome sequence except for the P1 region.P1^(GZL)induced a higher salicylic acid(SA)response than P1NB in Nicotiana benthamiana,which may explain the lower virus titers in plants infected with SMV^(GZL).Our results suggest that the divergence in the P1 proteins of these SMV isolates influenced their virulence via differentially regulating SA signaling.
