Bacterial diseases severely impact global agriculture,leading to substantial economic and yield losses.Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CLas),is a devastating disease,affecting cit...Bacterial diseases severely impact global agriculture,leading to substantial economic and yield losses.Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CLas),is a devastating disease,affecting citrus production worldwide(Graham et al.,2024).Likewise,bac-terial canker disease caused by Pseudomonas syringae pv.actinidiae(Psa)in kiwifruit(Actinidia spp.)resulted in yield reductions exceeding 80%,threatening industry collapse(Vanneste,2017).Current control strategies primarily rely on emergent chemical pesticides or the desperate yet common strategy of tree elimination and diseased-branch excision.展开更多
Infection and intoxication are two common types of foodborne illnesses throughout the world.The aim of the present work was to isolate and characterize biosurfactant producing bacteria fromgroundnut oil cake dumping s...Infection and intoxication are two common types of foodborne illnesses throughout the world.The aim of the present work was to isolate and characterize biosurfactant producing bacteria fromgroundnut oil cake dumping sites and evaluate their biocontrol effects against foodborne pathogens.Bacteria were isolated by enrichment culture technique and preliminary screening method.Biosurfactant activity evaluation was carried out by oil displacement,drop collapse test,lipase activity,hemolytic activity,emulsification index and emulsification assay methods.16s rRNA sequence analysis was used for the isolate identification.Crude biosurfactant was extracted by acid precipitation method and characterized using FTIR(Fourier Transform Infrared Spectroscopy).Antibacterial activity was investigated using disc diffusion method.CMCand surface reduction were analyzed by DuNouy tensiometer.Top 10 strains were selected for biosurfactant activity assessment from the total 30 isolates.In addition,16s rRNA sequence identified that the potential isolate was Bacillus pseudomycoides OR 1.Then,FTIR result of the extracted biosurfactant established the extract as a lipopeptide based on the absorption peaks at 3500 to 3200 cm^-1 and 2963 to 2854.68 cm^-1,respectively.50μg/mL of lipopeptide showed the highest antibacterial activity.Critical Micelle Concentration(CMC)of the lipopeptide was 60 mg/L and it reduced the surface tension of water from 71.6 to 31.6 mN/m.Hence,this study widens the scope to employ the bacterial lipopeptide surfactant as a promising biocontrol agent against foodborne pathogens.展开更多
Rice sheath blight is widely distributed throughout the world, it can cause damage on many kinds of crops. Pathogen characteristics, infection cycle, occurrence law, damage law and different control methods of rice sh...Rice sheath blight is widely distributed throughout the world, it can cause damage on many kinds of crops. Pathogen characteristics, infection cycle, occurrence law, damage law and different control methods of rice sheath blight in China are summarized, which will provide foundation for further worldwide research on the disease.展开更多
Pathogen and characteristics,occurrence law,damage symptom of Paulownia fortunei witches broom disease were simply described,and the control method of the disease was summarized.
Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was...Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was put into a heat treatment machine in pilot plan, and harmless condition in short time was examined. The results indicated, pathogenic indicator bacteria such as coliform bacteria, fecal coliform, Escherichia coli and salmonella were all 106 cfu/g dw at the beginning, died rapidly when cow dung temperature rose to above 50~C, and not detected at 54-68~C for 6-24 h heat treatment. Coliform bacteria and salmonella in heated cow dung were not detected by re-growth culture and enrichment culture examination. Moreover, it was hardly influenced on the fermentation ability of composting microbe, organic decomposition bacteria. During heat treatment, the mesophile decreased rapidly and the thermophile stabilized or increased, and the most of composting microbe were bacillus in cow dung by fluorescence microscope, this indicated that bacillus was dominator and composting microbe in composting process.展开更多
Nosocomial infections or healthcare associated infections occur in patients under medical care.These infections occur worldwide both in developed and developing countries.Nosocomial infections accounts for 7% in devel...Nosocomial infections or healthcare associated infections occur in patients under medical care.These infections occur worldwide both in developed and developing countries.Nosocomial infections accounts for 7% in developed and 10% in developing countries.As these infections occur during hospital stay,they cause prolonged stay,disability,and economic burden.Frequently prevalent infections include central line-associated bloodstream infections,catheter-associated urinary tract infections,surgical site infections and ventilator-associated pneumonia.Nosocomial pathogens include bacteria,viruses and fungal parasites.According to WHO estimates,approximately 15% of all hospitalized patients suffer from these infections.During hospitalization,patient is exposed to pathogens through different sources environment,healthcare staff,and other infected patients.Transmission of these infections should be restricted for prevention.Hospital waste serves as potential source of pathogens and about 20%–25% of hospital waste is termed as hazardous.Nosocomial infections can be controlled by practicing infection control programs,keep check on antimicrobial use and its resistance,adopting antibiotic control policy.Efficient surveillance system can play its part at national and international level.Efforts are required by all stakeholders to prevent and control nosocomial infections.展开更多
<strong>Objective: </strong>To investigate the etiology distribution and laboratory diagnosis of acute respiratory infectious diseases. <strong>Methods:</strong> By searching, collecting and su...<strong>Objective: </strong>To investigate the etiology distribution and laboratory diagnosis of acute respiratory infectious diseases. <strong>Methods:</strong> By searching, collecting and summarizing the etiology distribution and laboratory diagnosis of respiratory infectious diseases, the latest research progress of acute respiratory infectious diseases was studied in order to provide reference for clinical prevention and treatment. <strong>Results:</strong> In the relevant literature collected, it is considered that acute respiratory infectious diseases refer to infectious diseases caused by pathogens invading human body from the nasal cavity, throat, trachea and bronchus. The main acute respiratory infectious diseases are SARS (acute severe respiratory syndrome), MERS (Middle East respiratory syndrome), 2019-ncov infection (new coronavirus pneumonia), pulmonary plague, influenza, measles, diphtheria, pertussis, rubella, meningitis, mumps, tuberculosis, etc. Different types of infection have different diagnostic methods, and different treatments are given according to different diseases. <strong>Conclusion:</strong> The etiology distribution and laboratory diagnosis of acute respiratory tract infectious diseases are understood through reference, which can provide better reference for clinical practice. Acute respiratory infectious disease is the most common clinical disease, which seriously endangers people’s health.展开更多
Swine vesicular stomatitis is an acute, febrile, highly contagious zoonotic diseases caused by vesicular stomatitis virus （VSV）. Disease in affected pigs was typically characterized by vesicular lesions on the mouth...Swine vesicular stomatitis is an acute, febrile, highly contagious zoonotic diseases caused by vesicular stomatitis virus （VSV）. Disease in affected pigs was typically characterized by vesicular lesions on the mouth, snout, oral mucosa, coronet and interdigital skin. The disease is endemic in the Americas, Europe and Africa and other places. As the foreign trade of animals and animal products increase, the risk of swine vesicular stomatitis spreading into our country also will be increased. Therefore, the accurate and timely diagnosis and control of swine vesicular stomatitis are critical and necessary.展开更多
基金supported by the National Key R&D Program of China(2022YFD1400200)to L.H.
文摘Bacterial diseases severely impact global agriculture,leading to substantial economic and yield losses.Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CLas),is a devastating disease,affecting citrus production worldwide(Graham et al.,2024).Likewise,bac-terial canker disease caused by Pseudomonas syringae pv.actinidiae(Psa)in kiwifruit(Actinidia spp.)resulted in yield reductions exceeding 80%,threatening industry collapse(Vanneste,2017).Current control strategies primarily rely on emergent chemical pesticides or the desperate yet common strategy of tree elimination and diseased-branch excision.
文摘Infection and intoxication are two common types of foodborne illnesses throughout the world.The aim of the present work was to isolate and characterize biosurfactant producing bacteria fromgroundnut oil cake dumping sites and evaluate their biocontrol effects against foodborne pathogens.Bacteria were isolated by enrichment culture technique and preliminary screening method.Biosurfactant activity evaluation was carried out by oil displacement,drop collapse test,lipase activity,hemolytic activity,emulsification index and emulsification assay methods.16s rRNA sequence analysis was used for the isolate identification.Crude biosurfactant was extracted by acid precipitation method and characterized using FTIR(Fourier Transform Infrared Spectroscopy).Antibacterial activity was investigated using disc diffusion method.CMCand surface reduction were analyzed by DuNouy tensiometer.Top 10 strains were selected for biosurfactant activity assessment from the total 30 isolates.In addition,16s rRNA sequence identified that the potential isolate was Bacillus pseudomycoides OR 1.Then,FTIR result of the extracted biosurfactant established the extract as a lipopeptide based on the absorption peaks at 3500 to 3200 cm^-1 and 2963 to 2854.68 cm^-1,respectively.50μg/mL of lipopeptide showed the highest antibacterial activity.Critical Micelle Concentration(CMC)of the lipopeptide was 60 mg/L and it reduced the surface tension of water from 71.6 to 31.6 mN/m.Hence,this study widens the scope to employ the bacterial lipopeptide surfactant as a promising biocontrol agent against foodborne pathogens.
文摘Rice sheath blight is widely distributed throughout the world, it can cause damage on many kinds of crops. Pathogen characteristics, infection cycle, occurrence law, damage law and different control methods of rice sheath blight in China are summarized, which will provide foundation for further worldwide research on the disease.
文摘Pathogen and characteristics,occurrence law,damage symptom of Paulownia fortunei witches broom disease were simply described,and the control method of the disease was summarized.
文摘Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was put into a heat treatment machine in pilot plan, and harmless condition in short time was examined. The results indicated, pathogenic indicator bacteria such as coliform bacteria, fecal coliform, Escherichia coli and salmonella were all 106 cfu/g dw at the beginning, died rapidly when cow dung temperature rose to above 50~C, and not detected at 54-68~C for 6-24 h heat treatment. Coliform bacteria and salmonella in heated cow dung were not detected by re-growth culture and enrichment culture examination. Moreover, it was hardly influenced on the fermentation ability of composting microbe, organic decomposition bacteria. During heat treatment, the mesophile decreased rapidly and the thermophile stabilized or increased, and the most of composting microbe were bacillus in cow dung by fluorescence microscope, this indicated that bacillus was dominator and composting microbe in composting process.
文摘Nosocomial infections or healthcare associated infections occur in patients under medical care.These infections occur worldwide both in developed and developing countries.Nosocomial infections accounts for 7% in developed and 10% in developing countries.As these infections occur during hospital stay,they cause prolonged stay,disability,and economic burden.Frequently prevalent infections include central line-associated bloodstream infections,catheter-associated urinary tract infections,surgical site infections and ventilator-associated pneumonia.Nosocomial pathogens include bacteria,viruses and fungal parasites.According to WHO estimates,approximately 15% of all hospitalized patients suffer from these infections.During hospitalization,patient is exposed to pathogens through different sources environment,healthcare staff,and other infected patients.Transmission of these infections should be restricted for prevention.Hospital waste serves as potential source of pathogens and about 20%–25% of hospital waste is termed as hazardous.Nosocomial infections can be controlled by practicing infection control programs,keep check on antimicrobial use and its resistance,adopting antibiotic control policy.Efficient surveillance system can play its part at national and international level.Efforts are required by all stakeholders to prevent and control nosocomial infections.
文摘<strong>Objective: </strong>To investigate the etiology distribution and laboratory diagnosis of acute respiratory infectious diseases. <strong>Methods:</strong> By searching, collecting and summarizing the etiology distribution and laboratory diagnosis of respiratory infectious diseases, the latest research progress of acute respiratory infectious diseases was studied in order to provide reference for clinical prevention and treatment. <strong>Results:</strong> In the relevant literature collected, it is considered that acute respiratory infectious diseases refer to infectious diseases caused by pathogens invading human body from the nasal cavity, throat, trachea and bronchus. The main acute respiratory infectious diseases are SARS (acute severe respiratory syndrome), MERS (Middle East respiratory syndrome), 2019-ncov infection (new coronavirus pneumonia), pulmonary plague, influenza, measles, diphtheria, pertussis, rubella, meningitis, mumps, tuberculosis, etc. Different types of infection have different diagnostic methods, and different treatments are given according to different diseases. <strong>Conclusion:</strong> The etiology distribution and laboratory diagnosis of acute respiratory tract infectious diseases are understood through reference, which can provide better reference for clinical practice. Acute respiratory infectious disease is the most common clinical disease, which seriously endangers people’s health.
文摘Swine vesicular stomatitis is an acute, febrile, highly contagious zoonotic diseases caused by vesicular stomatitis virus （VSV）. Disease in affected pigs was typically characterized by vesicular lesions on the mouth, snout, oral mucosa, coronet and interdigital skin. The disease is endemic in the Americas, Europe and Africa and other places. As the foreign trade of animals and animal products increase, the risk of swine vesicular stomatitis spreading into our country also will be increased. Therefore, the accurate and timely diagnosis and control of swine vesicular stomatitis are critical and necessary.
