Objective: investigate the pathogenesis of acute coronary syndrome (ACS) with paroxysmal atrial fibrillation, and to provide theoretical basis for subsequent treatment and prevention. Methods: A 72-year-old female pat...Objective: investigate the pathogenesis of acute coronary syndrome (ACS) with paroxysmal atrial fibrillation, and to provide theoretical basis for subsequent treatment and prevention. Methods: A 72-year-old female patient with persistent "paroxysm chest pain" who was admitted to hospital at 15:00, 2020.03.17.00, was given physical examination immediately after admission, and performed routine blood, urine, stool, liver function, kidney function, electrolyte, coagulation series, D-dimer and other laboratory tests, followed by electrocardiogram, cardiac ultrasonography and coronary angiography. Results: the laboratory examination showed no obvious abnormality. ECG showed sinus rhythm, heart rate 76 beats/min. The color ultrasonography of the heart showed aorta sclerosis with no abnormal color blood flow. The coronary artery presented the dominant type of right coronary artery, with different degrees of stenosis at the opening, the middle segment, the proximal segment of the left ventricular posterior branch, the proximal segment of the anterior descending branch and the distal segment. Conclusion: the patient had coronary heart disease with unstable angina pectoris, type-2 diabetes mellitus and grade 3-level hypertension. The whole course of the patient's disease was consistent with the acute coronary syndrome (ACS) induced by paroxysmal atrial fibrillation (AF) and the specific clinical presentation was unstable angina pectoris.展开更多
Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 （a reassortant of G1 and G9 viruses isolated from a female patient in 1999） in a mouse model of infection.Metho...Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 （a reassortant of G1 and G9 viruses isolated from a female patient in 1999） in a mouse model of infection.Methods Mice were infected with increasing virus titers.Viral load in the lungs and trachea was determined by EID50 assay.Pulmonary histopathology was assessed by hematoxylin‐eosin staining.Anti‐HI antibody titers and T‐cell responses to viral HA were determined by ELISPOT and confirmed by flow cytometry.Results Mice presented a mild syndrome after intranasal infection with A/Guangzhou/333/99 （H9N2） influenza virus.Virus was detected in the trachea and lungs of mice harvested on days 3,6,and 9 post‐infection.A T‐cell response to viral HA was detected on day 6 and H9 HA‐specific CD 4＋ T‐cells predominated.Seroconversion was detected after 14 days and antibody persisted for at least 28 weeks.Conclusion Our results suggest that H9N2 （A/Guangzhou/333/99） can replicate in the murine respiratory tract without prior adaptation,and both humoral and cell‐mediated immunity play an important role in the immune response.展开更多
The pathogen, characteristics, pathogenic mechanism, infection cycle, occurrence regularity and damage symptom of cotton Verticillium wilt are expounded in the paper. The paper puts forward the strategy of protecting ...The pathogen, characteristics, pathogenic mechanism, infection cycle, occurrence regularity and damage symptom of cotton Verticillium wilt are expounded in the paper. The paper puts forward the strategy of protecting disease-free area first, planting resistant varieties and rational rotation in severe area and strengthening the field management, as well as combining with biological control and chemical control methods to effectively control infection and spread of pathogen, so as to provide certain theoretical basis for reducing the damage of cotton Verticillium wilt and improving yield and quality of cotton.展开更多
Introduction:Aeromonas is widely distributed in aquatic environments.This study describes the pathogenic characteristics of Aeromonas isolated from adult diarrhea patients and aquatic environments in Beijing,China.Met...Introduction:Aeromonas is widely distributed in aquatic environments.This study describes the pathogenic characteristics of Aeromonas isolated from adult diarrhea patients and aquatic environments in Beijing,China.Methods:Aeromonas isolates from patients with diarrhea and river water samples were assessed using whole-genome sequencing(WGS)and antibiotic resistance profiling.Results:In total,38 Aeromonas isolates were collected.Among these,13 isolates were from patients with common clinical symptoms,including diarrhea,abdominal pain,and nausea.Four of 13 Aeromonaspositive patients were co-infected with other intestinal pathogens.Patient-derived Aeromonas isolates showed high resistance to ampicillin,ampicillin-sulbactam,nalidixic acid,and tetracycline,whereas water-derived isolates showed high resistance to ampicillin,ampicillin-sulbactam,and nalidixic acid.Phylogenetic analysis revealed seven independent branches,without significant clustering among patient-and water-derived isolates.Conclusions:This study provides valuable insights into the prevalence and characteristics of Aeromonas.The intertwined distribution of patient-and waterderived isolates in the phylogenetic tree deserves attention.展开更多
Introduction:This study aims to analyze the clinical characteristics and pathogenic features of Chlamydia pneumoniae pneumonia,providing a scientific basis for the diagnosis and treatment of C.pneumoniae infections.Me...Introduction:This study aims to analyze the clinical characteristics and pathogenic features of Chlamydia pneumoniae pneumonia,providing a scientific basis for the diagnosis and treatment of C.pneumoniae infections.Methods:Clinical data from four patients diagnosed with C.pneumoniae pneumonia in Lishui City,Zhejiang Province,between April and May 2024,were collected to analyze clinical manifestations and pathogenic findings.Multi-locus sequence typing(MLST)analysis of the pathogen was conducted using seven housekeeping genes.Results:All patients exhibited decreased levels of retinol-binding protein and prealbumin,findings not previously reported in earlier studies.Additionally,coinfections were identified in two cases.Analysis of the 16S rRNA and ompA gene sequences indicated a homology of 98%to 100%with known C.pneumoniae strains.To further characterize these strains,sequencing of the seven housekeeping genes confirmed that all cases were infected with the ST16 genotype.Conclusions:C.pneumoniae infections in Lishui City are predominantly caused by the ST16 genotype,highlighting the need for enhanced research into these infections.The decrease in retinol-binding protein and prealbumin levels may serve as auxiliary diagnostic biomarkers in clinical practice.Next-generation sequencing methods demonstrate significant potential for pathogen identification,particularly in diagnosing co-infections.展开更多
The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distri...The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distribution of C. trachomatis and other pathogenic microorganisms, to detect the isolation rate of infectious pathogens, and to provide an evidence for further targeted efforts in the prevent of sporadic trachoma efforts. From two primary schools in Qinghai Province, ocular samples from 35 students who were clinically diagnosed as trachoma cases and 60 normal controls were obtained by swabbing their upper eyelids and lower conjunctival sacs. Samples were preserved at 4°C and airlifted to Beijing Tongren Hospital within 24 h. Real-time polymerase chain reaction(RT-PCR) was used to screen for C. trachomatis, and nested PCR was used to amplify a fragment of the omp A gene for serotype confirmation. Bacterial cultivation and sensitivity tests were conducted based on the 2015 version of the Clinical and Laboratory Standards Institute. Adenovirus, herpes simplex virus, cytomegalovirus, and Epstein-Barr virus were screened by RT-PCR. Among the 35 students with trachoma, 8 came from the Jianshetang Primary School and 27 came from the Central Primary School. Two novel C. trachomatis B serotypes(Gen Bank accession numbers KU737520 and KU737521) were detected based on a sequence analysis of the omp A gene. Single C. trachomatis infections accounted for 42.86%(9/21) of the cases, and infections with multiple bacteria, particularly Haemophilus influenzae, Staphylococcus aureus, Moraxella catarrhalis, and Streptococcus pneumoniae, accounted for the remaining 57.14%(12/21). Of the 14 C. trachomatis-negative samples, one was positive for adenoviral infection(serotype D) and 13 were positive for bacterial infections(H. influenzae, M. catarrhalis, S. pneumoniae, S. aureus, streptococci other than S. pneumoniae, Staphylococcus epidermidis, Corynebacterium, and Arthrobacterium). In addition to C. trachomatis, the other bacteria and virus that were detected in the boarding students of primary schools in the Qinghai Tibetan area should be emphasized in trachoma prevention and control.展开更多
文摘Objective: investigate the pathogenesis of acute coronary syndrome (ACS) with paroxysmal atrial fibrillation, and to provide theoretical basis for subsequent treatment and prevention. Methods: A 72-year-old female patient with persistent "paroxysm chest pain" who was admitted to hospital at 15:00, 2020.03.17.00, was given physical examination immediately after admission, and performed routine blood, urine, stool, liver function, kidney function, electrolyte, coagulation series, D-dimer and other laboratory tests, followed by electrocardiogram, cardiac ultrasonography and coronary angiography. Results: the laboratory examination showed no obvious abnormality. ECG showed sinus rhythm, heart rate 76 beats/min. The color ultrasonography of the heart showed aorta sclerosis with no abnormal color blood flow. The coronary artery presented the dominant type of right coronary artery, with different degrees of stenosis at the opening, the middle segment, the proximal segment of the left ventricular posterior branch, the proximal segment of the anterior descending branch and the distal segment. Conclusion: the patient had coronary heart disease with unstable angina pectoris, type-2 diabetes mellitus and grade 3-level hypertension. The whole course of the patient's disease was consistent with the acute coronary syndrome (ACS) induced by paroxysmal atrial fibrillation (AF) and the specific clinical presentation was unstable angina pectoris.
基金supported by the National Basic Research Program of China (973 program: 2005CB523006)
文摘Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 （a reassortant of G1 and G9 viruses isolated from a female patient in 1999） in a mouse model of infection.Methods Mice were infected with increasing virus titers.Viral load in the lungs and trachea was determined by EID50 assay.Pulmonary histopathology was assessed by hematoxylin‐eosin staining.Anti‐HI antibody titers and T‐cell responses to viral HA were determined by ELISPOT and confirmed by flow cytometry.Results Mice presented a mild syndrome after intranasal infection with A/Guangzhou/333/99 （H9N2） influenza virus.Virus was detected in the trachea and lungs of mice harvested on days 3,6,and 9 post‐infection.A T‐cell response to viral HA was detected on day 6 and H9 HA‐specific CD 4＋ T‐cells predominated.Seroconversion was detected after 14 days and antibody persisted for at least 28 weeks.Conclusion Our results suggest that H9N2 （A/Guangzhou/333/99） can replicate in the murine respiratory tract without prior adaptation,and both humoral and cell‐mediated immunity play an important role in the immune response.
基金Supported by National Natural Foundation of China (41161068)Technology Guiding Program of Xinjiang Academy of Land Reclamation Science(YYD201102)
文摘The pathogen, characteristics, pathogenic mechanism, infection cycle, occurrence regularity and damage symptom of cotton Verticillium wilt are expounded in the paper. The paper puts forward the strategy of protecting disease-free area first, planting resistant varieties and rational rotation in severe area and strengthening the field management, as well as combining with biological control and chemical control methods to effectively control infection and spread of pathogen, so as to provide certain theoretical basis for reducing the damage of cotton Verticillium wilt and improving yield and quality of cotton.
基金Supported by the National Key Research Program of China(2021YFF0703801).
文摘Introduction:Aeromonas is widely distributed in aquatic environments.This study describes the pathogenic characteristics of Aeromonas isolated from adult diarrhea patients and aquatic environments in Beijing,China.Methods:Aeromonas isolates from patients with diarrhea and river water samples were assessed using whole-genome sequencing(WGS)and antibiotic resistance profiling.Results:In total,38 Aeromonas isolates were collected.Among these,13 isolates were from patients with common clinical symptoms,including diarrhea,abdominal pain,and nausea.Four of 13 Aeromonaspositive patients were co-infected with other intestinal pathogens.Patient-derived Aeromonas isolates showed high resistance to ampicillin,ampicillin-sulbactam,nalidixic acid,and tetracycline,whereas water-derived isolates showed high resistance to ampicillin,ampicillin-sulbactam,and nalidixic acid.Phylogenetic analysis revealed seven independent branches,without significant clustering among patient-and water-derived isolates.Conclusions:This study provides valuable insights into the prevalence and characteristics of Aeromonas.The intertwined distribution of patient-and waterderived isolates in the phylogenetic tree deserves attention.
基金supported by grants from the Tengfei initiative by the National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases(grant number 2024NITFID504)grants from the National Key Research and Development Program of China(grant number 2021YFC2301202)the National Natural Science Foundation of China(grant number 82102390).
文摘Introduction:This study aims to analyze the clinical characteristics and pathogenic features of Chlamydia pneumoniae pneumonia,providing a scientific basis for the diagnosis and treatment of C.pneumoniae infections.Methods:Clinical data from four patients diagnosed with C.pneumoniae pneumonia in Lishui City,Zhejiang Province,between April and May 2024,were collected to analyze clinical manifestations and pathogenic findings.Multi-locus sequence typing(MLST)analysis of the pathogen was conducted using seven housekeeping genes.Results:All patients exhibited decreased levels of retinol-binding protein and prealbumin,findings not previously reported in earlier studies.Additionally,coinfections were identified in two cases.Analysis of the 16S rRNA and ompA gene sequences indicated a homology of 98%to 100%with known C.pneumoniae strains.To further characterize these strains,sequencing of the seven housekeeping genes confirmed that all cases were infected with the ST16 genotype.Conclusions:C.pneumoniae infections in Lishui City are predominantly caused by the ST16 genotype,highlighting the need for enhanced research into these infections.The decrease in retinol-binding protein and prealbumin levels may serve as auxiliary diagnostic biomarkers in clinical practice.Next-generation sequencing methods demonstrate significant potential for pathogen identification,particularly in diagnosing co-infections.
文摘The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distribution of C. trachomatis and other pathogenic microorganisms, to detect the isolation rate of infectious pathogens, and to provide an evidence for further targeted efforts in the prevent of sporadic trachoma efforts. From two primary schools in Qinghai Province, ocular samples from 35 students who were clinically diagnosed as trachoma cases and 60 normal controls were obtained by swabbing their upper eyelids and lower conjunctival sacs. Samples were preserved at 4°C and airlifted to Beijing Tongren Hospital within 24 h. Real-time polymerase chain reaction(RT-PCR) was used to screen for C. trachomatis, and nested PCR was used to amplify a fragment of the omp A gene for serotype confirmation. Bacterial cultivation and sensitivity tests were conducted based on the 2015 version of the Clinical and Laboratory Standards Institute. Adenovirus, herpes simplex virus, cytomegalovirus, and Epstein-Barr virus were screened by RT-PCR. Among the 35 students with trachoma, 8 came from the Jianshetang Primary School and 27 came from the Central Primary School. Two novel C. trachomatis B serotypes(Gen Bank accession numbers KU737520 and KU737521) were detected based on a sequence analysis of the omp A gene. Single C. trachomatis infections accounted for 42.86%(9/21) of the cases, and infections with multiple bacteria, particularly Haemophilus influenzae, Staphylococcus aureus, Moraxella catarrhalis, and Streptococcus pneumoniae, accounted for the remaining 57.14%(12/21). Of the 14 C. trachomatis-negative samples, one was positive for adenoviral infection(serotype D) and 13 were positive for bacterial infections(H. influenzae, M. catarrhalis, S. pneumoniae, S. aureus, streptococci other than S. pneumoniae, Staphylococcus epidermidis, Corynebacterium, and Arthrobacterium). In addition to C. trachomatis, the other bacteria and virus that were detected in the boarding students of primary schools in the Qinghai Tibetan area should be emphasized in trachoma prevention and control.
