The microbial induced calcium carbonate precipitation(MICP)technology provides a new approach to solve borehole destabilization in broken formations;however,the high-temperature and alkaline environments inhibit the g...The microbial induced calcium carbonate precipitation(MICP)technology provides a new approach to solve borehole destabilization in broken formations;however,the high-temperature and alkaline environments inhibit the growth of microorganisms,which in turn affects the performance of their wall enhancement performance.In this study,a pH and temperature-coupled induced domestication method was applied to Bacillus pasteurii,and its wall enhancement performance was evaluated.Post domestication,Bacillus pasteurii exhibited high growth activity at pH 10.3 and temperature 45℃.In a sodium carboxymethyl cellulose(CMC)drilling fluid environment,bacterial concentration reached 1.373 with urease activity at 1.98 after 24 h,and in a xanthan gum(XG)environment,the figures were 0.931 and 1.76,respectively—significantly higher than those before domestication.The Bacillus pasteurii-CMC system exhibited enhanced performance with the unconfined compressive strength of the specimen up to 1.232 MPa,permeability coefficient as low as 0.024,and calcium carbonate production up to 24.685 g.The crushed specimen portions remained lumpy with even calcium carbonate distribution.In contrast,the Bacillus pasteurii-XG system exhibited the highest unconfined compressive strength of 0.561 MPa,lowest permeability coefficient of 0.081,and the greatest calcium carbonate production of 16.03 g,with an externally cemented shell but internally loose structure and uneven calcium carbonate distribution,resulting in weaker mechanical properties.The Bacillus pasteurii induced predominantly vaterite calcium carbonate crystals in the CMC drilling fluid.In the XG drilling fluid,the crystals were mainly calcite.Both types effectively cemented the broken particles,improving formation strength and reducing permeability.However,under the same conditions,the Bacillus pasteurii-CMC system demonstrated a more pronounced enhancement effect.展开更多
The application of the microbially induced carbonate precipitation(MICP)method for remediating heavy metals(i.e.,HMs)has recently garnered significant attention.Nevertheless,the inhibition of urease activity by toxic ...The application of the microbially induced carbonate precipitation(MICP)method for remediating heavy metals(i.e.,HMs)has recently garnered significant attention.Nevertheless,the inhibition of urease activity by toxic Cd^(2+),Pb^(2+),Zn^(2+),and Cu^(2+)poses a challenge for MICP-based remediation of HMs contamination.This study:(1)first performed the traditional MICP tests(in which the bacterial solution,urea solution,and HMs were mixed simultaneously),and investigated the toxic effect of HMs on the urease activity and the immobilization efficiency,(2)analyzed the toxicity and immobilization mechanism during the MICP process by combining the simulation and XRD tests,(3)conducted the two-step MICP tests(which initially mixed the bacterial solution and urea solution to promote urea hydrolysis,then added the HMs solutions for HMs precipitation)to improve the immobilization efficiency.The tube experiments and simulations were investigated in the HMs concentration range from 1 to 10 mmol/L.Indicators including am-monium concentration,HMs concentrations,and pH were measured/recorded during the tests.The results show that soluble HMs exhibit a concentration-dependent inhibition of urea hydrolysis during the traditional MICP process,resulting in a decreasing immobilization efficiency.The two-step MICP method can effectively immobilize almost the Cd^(2+)and Zn^(2+)when the initial urea hydrolysis period exceeds 1-2 h.In addition,a high immobilization rate of over 90% can be achieved for Cu-contaminated solutions at the optimal first-step reaction time.Compared with the traditional MICP procedure,the effective two-step MICP method exhibits more promising application prospects for the immobilization of soluble HMs in aquatic environments.展开更多
The antibacterial activity of Whey Milk and its fractions were studied against one hundred bacteria strains isolated and screened from various patients at the University Hospital of Zagazig,Sharkia Province,Egypt.F2 f...The antibacterial activity of Whey Milk and its fractions were studied against one hundred bacteria strains isolated and screened from various patients at the University Hospital of Zagazig,Sharkia Province,Egypt.F2 fraction of Whey Milk has shown the highest degree of antibacterial activity against the pathogenic bacteria A63 isolated from the wound.Buffalo whey(BW)was hydrolyzed with papain followed by fractionation using size exclusion chromatography(SEC).The fractionation profile of BWH showed three major peaks in Size exclusion chromatography,SECF1,SECF2,and SECF3 after 3h.F2 is the most active fraction and has faster migration than HP,F1,and F3 and may have higher positive charges on Urea-PAGE towards the cathode direction.FTIR spectrum analysis of the F2 fraction of whey protein demonstrates the occurrence of distinctive functional groups,such as Alkanes,alcohols,phenols,flavonoids,alkenes,anhydrides,and halo compounds.The biochemical characters and the 1.5 Kb 16S RNA gene’s nucleotide sequence of the most potent stain showed 99%similarity with Staphylococcus pasteuri.Our results showed that the ability of nanoparticles of whey to inhibit pathogenic bacteria decreases compared to raw whey and its fractions.Cellular deformation and partial and total lysis of cell components were seen in Staphylococcus pasteuri treated with whey F2 fraction under Transmission Electron Microscopy(TEM).展开更多
基金supported by the State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Independent Research Project(SKLGP2023Z012)the National Natural Science Foundation of China(Grant No.41702388)the Everest Technology Research Proposal of Chengdu University of Technology(Grant No.80000-2023ZF11411)。
文摘The microbial induced calcium carbonate precipitation(MICP)technology provides a new approach to solve borehole destabilization in broken formations;however,the high-temperature and alkaline environments inhibit the growth of microorganisms,which in turn affects the performance of their wall enhancement performance.In this study,a pH and temperature-coupled induced domestication method was applied to Bacillus pasteurii,and its wall enhancement performance was evaluated.Post domestication,Bacillus pasteurii exhibited high growth activity at pH 10.3 and temperature 45℃.In a sodium carboxymethyl cellulose(CMC)drilling fluid environment,bacterial concentration reached 1.373 with urease activity at 1.98 after 24 h,and in a xanthan gum(XG)environment,the figures were 0.931 and 1.76,respectively—significantly higher than those before domestication.The Bacillus pasteurii-CMC system exhibited enhanced performance with the unconfined compressive strength of the specimen up to 1.232 MPa,permeability coefficient as low as 0.024,and calcium carbonate production up to 24.685 g.The crushed specimen portions remained lumpy with even calcium carbonate distribution.In contrast,the Bacillus pasteurii-XG system exhibited the highest unconfined compressive strength of 0.561 MPa,lowest permeability coefficient of 0.081,and the greatest calcium carbonate production of 16.03 g,with an externally cemented shell but internally loose structure and uneven calcium carbonate distribution,resulting in weaker mechanical properties.The Bacillus pasteurii induced predominantly vaterite calcium carbonate crystals in the CMC drilling fluid.In the XG drilling fluid,the crystals were mainly calcite.Both types effectively cemented the broken particles,improving formation strength and reducing permeability.However,under the same conditions,the Bacillus pasteurii-CMC system demonstrated a more pronounced enhancement effect.
基金supported by the Key research and development project of Hubei Province(No.2022BAA068)the National Natural Science Foundation of China(NSFC)(No.52122806,51978303)+1 种基金the Fundamental Research Funds for the Central Universities(No.2042023kfyq03)Joint fund of the technical R&D program of Henan Province(No.225200810005).
文摘The application of the microbially induced carbonate precipitation(MICP)method for remediating heavy metals(i.e.,HMs)has recently garnered significant attention.Nevertheless,the inhibition of urease activity by toxic Cd^(2+),Pb^(2+),Zn^(2+),and Cu^(2+)poses a challenge for MICP-based remediation of HMs contamination.This study:(1)first performed the traditional MICP tests(in which the bacterial solution,urea solution,and HMs were mixed simultaneously),and investigated the toxic effect of HMs on the urease activity and the immobilization efficiency,(2)analyzed the toxicity and immobilization mechanism during the MICP process by combining the simulation and XRD tests,(3)conducted the two-step MICP tests(which initially mixed the bacterial solution and urea solution to promote urea hydrolysis,then added the HMs solutions for HMs precipitation)to improve the immobilization efficiency.The tube experiments and simulations were investigated in the HMs concentration range from 1 to 10 mmol/L.Indicators including am-monium concentration,HMs concentrations,and pH were measured/recorded during the tests.The results show that soluble HMs exhibit a concentration-dependent inhibition of urea hydrolysis during the traditional MICP process,resulting in a decreasing immobilization efficiency.The two-step MICP method can effectively immobilize almost the Cd^(2+)and Zn^(2+)when the initial urea hydrolysis period exceeds 1-2 h.In addition,a high immobilization rate of over 90% can be achieved for Cu-contaminated solutions at the optimal first-step reaction time.Compared with the traditional MICP procedure,the effective two-step MICP method exhibits more promising application prospects for the immobilization of soluble HMs in aquatic environments.
基金funded by institutional fund projects under no(IFP-A-2022-2-5-22)technical and financial support from the Ministry of Education Saudi Arabia.
文摘The antibacterial activity of Whey Milk and its fractions were studied against one hundred bacteria strains isolated and screened from various patients at the University Hospital of Zagazig,Sharkia Province,Egypt.F2 fraction of Whey Milk has shown the highest degree of antibacterial activity against the pathogenic bacteria A63 isolated from the wound.Buffalo whey(BW)was hydrolyzed with papain followed by fractionation using size exclusion chromatography(SEC).The fractionation profile of BWH showed three major peaks in Size exclusion chromatography,SECF1,SECF2,and SECF3 after 3h.F2 is the most active fraction and has faster migration than HP,F1,and F3 and may have higher positive charges on Urea-PAGE towards the cathode direction.FTIR spectrum analysis of the F2 fraction of whey protein demonstrates the occurrence of distinctive functional groups,such as Alkanes,alcohols,phenols,flavonoids,alkenes,anhydrides,and halo compounds.The biochemical characters and the 1.5 Kb 16S RNA gene’s nucleotide sequence of the most potent stain showed 99%similarity with Staphylococcus pasteuri.Our results showed that the ability of nanoparticles of whey to inhibit pathogenic bacteria decreases compared to raw whey and its fractions.Cellular deformation and partial and total lysis of cell components were seen in Staphylococcus pasteuri treated with whey F2 fraction under Transmission Electron Microscopy(TEM).
