Steroidal saponins are major bioactive compounds of the medicinal plant Paris polyphylla var.yunnanensis.In this work,two O-rhamnosyltransferases Pp Rha GT1 and Pp Rha GT2 with strict substrate specificity were charac...Steroidal saponins are major bioactive compounds of the medicinal plant Paris polyphylla var.yunnanensis.In this work,two O-rhamnosyltransferases Pp Rha GT1 and Pp Rha GT2 with strict substrate specificity were characterized from this plant.These enzymes could catalyze the synthesis of paris saponinsⅡandⅦ,and realized semi-biosynthesis of a series of paris steroidal saponins in tobacco leaves.Molecular dynamics simulation revealed the substrate specificity of Pp Rha GT1 was due to interactions between the 2-O-rhamnosyl group and surrounding amino acids particularly S382 and E383.展开更多
To ensure the safety and efficacy of Chinese herbs,it is of great significance to conduct rapid quality detection of Chinese herbs at every link of their supply chain.Spectroscopic technology can reflect the overall c...To ensure the safety and efficacy of Chinese herbs,it is of great significance to conduct rapid quality detection of Chinese herbs at every link of their supply chain.Spectroscopic technology can reflect the overall chemical composition and structural characteristics of Chinese herbs,with the multi-component and multitarget characteristics of Chinese herbs.This review took the genus Paris as an example,and applications of spectroscopic technology with machine learning(ML)in supply chain of the genus Paris from seeds to medicinal materials were introduced.The specific contents included the confirmation of germplasm resources,identification of growth years,cultivar,geographical origin,and original processing and processing methods.The potential application of spectroscopic technology in genus Paris was pointed out,and the prospects of combining spectroscopic technology with blockchain were proposed.The summary and prospects presented in this paper will be beneficial to the quality control of the genus Paris in all links of its supply chain,so as to rationally use the genus Paris resources and ensure the safety and efficacy of medication.展开更多
Lignite provides energy security and contributes economically.However,it also causes dirty outcomes in terms of climate aspect.In addition to the energy and climate dimensions of the Sustainable Development Goals,ther...Lignite provides energy security and contributes economically.However,it also causes dirty outcomes in terms of climate aspect.In addition to the energy and climate dimensions of the Sustainable Development Goals,there is also a water issue:lignite is usually found submerged below the local groundwater tables.Mining lignite could be exploited to achieve drinkable and agriculturally usable water.In today’s literature,while the impact of lignite production on global warming and emissions are already highly discussed,the water management side of the issue is regularly omitted.However,considering the complex interlink between these three areas(the Water-Energy-Climate(WEC)nexus)is necessary within policy coherence,which is mostly ignored even though it is one of the development targets.Here in this framework,Turkiye,which aims to reduce its heavy dependency on energy imports,is worth studying because almost all of its coal,the country’s largest fossil resource,is lignite.Therefore,this study examines the WEC nexus related to lignite production and combustion and seeks policy coherence between their outputs in the context of Turkiye’s historical steps to climate change mitigation,specifically oriented with the Paris Agreement.This story expands from the absence of specific development policy objectives to the practicalities of politics and economics.展开更多
A novel phenylpropanoid glycosides 1, named parispolyside E and a novel derivation of phenolic glycoside 2, named parispolyside G, as well as two known flavonoid glycosides were isolated from the rhizome of Paris poly...A novel phenylpropanoid glycosides 1, named parispolyside E and a novel derivation of phenolic glycoside 2, named parispolyside G, as well as two known flavonoid glycosides were isolated from the rhizome of Paris polyphylla var. yunnanensis. Their structures were elucidaed by spectroscopic methods.展开更多
Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compou...Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compounds.Their structures were established mainly on the base of NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.In addition,the cytotoxicity of the two new saponins was tested.Graphical Abstract Two new highly oxygenated spirostanol saponins,paristenosides A(1)and B(2),were isolated from the rhizomes of Paris polyphylla var.stenophylla.Their structures were established mainly based on NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.展开更多
Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Pa...Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 （ 150 mm× 4.6 mm ID, 5μm） column which was subjected to gradient elution with acetonitrile-water （30：70- 60：40, V/V） at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy.展开更多
This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, incre...This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, increasing income of foresters and protecting ecological environment. Three types of fores{ land, i.e., the evergreen forest land （China fir forest）, deciduous forest land （walnut forest） and shrub land （honey suckle forest.） were selected, and seeds and cut rhizomes of Paris polyphylla Smith produced in Bamian Mountain, Hunan, were used in the study on understory seedling raising and cultivation techniques. The results showed that： when using different propagation materials, seed propagation could be adopted to reduce the consumption of wild resources and provide a large number of seedlings, and was beneficial to large-scale planting; and when conducing seedling and plantlet raising followed by transplanting and direct seeding and planting using different propagation materials in large-scale understory cultivation, direct seeding/ planting was appropriate for saving save production cost with the advantage of early production. Among the 3 different types of forest land, the best choice was broad- leaved forest land, which could achieve better economic benefit because of con- forming with the ecological habit and growth and development law of Paris poly- phylla Smith.展开更多
基金supported by Yunnan Provincial Science and Technology Project at Southwest United Graduate School(No.202302AP370006)the National Key Research and Development Program of China(No.2023YFA0914100)。
文摘Steroidal saponins are major bioactive compounds of the medicinal plant Paris polyphylla var.yunnanensis.In this work,two O-rhamnosyltransferases Pp Rha GT1 and Pp Rha GT2 with strict substrate specificity were characterized from this plant.These enzymes could catalyze the synthesis of paris saponinsⅡandⅦ,and realized semi-biosynthesis of a series of paris steroidal saponins in tobacco leaves.Molecular dynamics simulation revealed the substrate specificity of Pp Rha GT1 was due to interactions between the 2-O-rhamnosyl group and surrounding amino acids particularly S382 and E383.
基金funded by the Special Program for the Major Science and Technology Projects of Yunnan Province,China(Grant No.:202202AE090001).
文摘To ensure the safety and efficacy of Chinese herbs,it is of great significance to conduct rapid quality detection of Chinese herbs at every link of their supply chain.Spectroscopic technology can reflect the overall chemical composition and structural characteristics of Chinese herbs,with the multi-component and multitarget characteristics of Chinese herbs.This review took the genus Paris as an example,and applications of spectroscopic technology with machine learning(ML)in supply chain of the genus Paris from seeds to medicinal materials were introduced.The specific contents included the confirmation of germplasm resources,identification of growth years,cultivar,geographical origin,and original processing and processing methods.The potential application of spectroscopic technology in genus Paris was pointed out,and the prospects of combining spectroscopic technology with blockchain were proposed.The summary and prospects presented in this paper will be beneficial to the quality control of the genus Paris in all links of its supply chain,so as to rationally use the genus Paris resources and ensure the safety and efficacy of medication.
文摘Lignite provides energy security and contributes economically.However,it also causes dirty outcomes in terms of climate aspect.In addition to the energy and climate dimensions of the Sustainable Development Goals,there is also a water issue:lignite is usually found submerged below the local groundwater tables.Mining lignite could be exploited to achieve drinkable and agriculturally usable water.In today’s literature,while the impact of lignite production on global warming and emissions are already highly discussed,the water management side of the issue is regularly omitted.However,considering the complex interlink between these three areas(the Water-Energy-Climate(WEC)nexus)is necessary within policy coherence,which is mostly ignored even though it is one of the development targets.Here in this framework,Turkiye,which aims to reduce its heavy dependency on energy imports,is worth studying because almost all of its coal,the country’s largest fossil resource,is lignite.Therefore,this study examines the WEC nexus related to lignite production and combustion and seeks policy coherence between their outputs in the context of Turkiye’s historical steps to climate change mitigation,specifically oriented with the Paris Agreement.This story expands from the absence of specific development policy objectives to the practicalities of politics and economics.
文摘A novel phenylpropanoid glycosides 1, named parispolyside E and a novel derivation of phenolic glycoside 2, named parispolyside G, as well as two known flavonoid glycosides were isolated from the rhizome of Paris polyphylla var. yunnanensis. Their structures were elucidaed by spectroscopic methods.
基金This work is funded by the National Natural Science Funding of China(Grand No.31570363)the Key Research Program of the Chinese Academy of Sciences(Grand No.KSZDEW-Z-004-03-4)the Natural Science Foundation of Yunnan Province(Grand No.2015FA031).
文摘Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compounds.Their structures were established mainly on the base of NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.In addition,the cytotoxicity of the two new saponins was tested.Graphical Abstract Two new highly oxygenated spirostanol saponins,paristenosides A(1)and B(2),were isolated from the rhizomes of Paris polyphylla var.stenophylla.Their structures were established mainly based on NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.
文摘Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 （ 150 mm× 4.6 mm ID, 5μm） column which was subjected to gradient elution with acetonitrile-water （30：70- 60：40, V/V） at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy.
基金Supported by Central Financial Forestry Science and Technology Promotion project([2014]XT003)~~
文摘This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, increasing income of foresters and protecting ecological environment. Three types of fores{ land, i.e., the evergreen forest land （China fir forest）, deciduous forest land （walnut forest） and shrub land （honey suckle forest.） were selected, and seeds and cut rhizomes of Paris polyphylla Smith produced in Bamian Mountain, Hunan, were used in the study on understory seedling raising and cultivation techniques. The results showed that： when using different propagation materials, seed propagation could be adopted to reduce the consumption of wild resources and provide a large number of seedlings, and was beneficial to large-scale planting; and when conducing seedling and plantlet raising followed by transplanting and direct seeding and planting using different propagation materials in large-scale understory cultivation, direct seeding/ planting was appropriate for saving save production cost with the advantage of early production. Among the 3 different types of forest land, the best choice was broad- leaved forest land, which could achieve better economic benefit because of con- forming with the ecological habit and growth and development law of Paris poly- phylla Smith.
