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猪圆环病毒4型Cap蛋白的原核表达及多克隆抗体制备
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作者 文英会 崔鸿博 +5 位作者 陈曦艋 陈红英 李金磊 赵丽 刘占通 闫志浩 《中国畜牧兽医》 北大核心 2025年第10期4904-4911,共8页
【目的】通过原核表达系统表达猪圆环病毒4型(Porcine circovirus type 4,PCV4)衣壳蛋白(Cap),并制备其多克隆抗体,为PCV4的防控提供技术支撑。【方法】基于PCV4开放阅读框2(ORF2)基因的免疫原区域设计1对特异性引物,通过PCR扩增获得PCV... 【目的】通过原核表达系统表达猪圆环病毒4型(Porcine circovirus type 4,PCV4)衣壳蛋白(Cap),并制备其多克隆抗体,为PCV4的防控提供技术支撑。【方法】基于PCV4开放阅读框2(ORF2)基因的免疫原区域设计1对特异性引物,通过PCR扩增获得PCV4 ORF2基因序列,并将其连接至pET-28a(+)载体,构建重组表达质粒pET-28a-ORF2;将经PCR和测序鉴定正确的重组质粒pET-28a-ORF2转化大肠杆菌BL21(DE3)感受态细胞,经1 mmol/L IPTG诱导表达重组Cap蛋白,利用SDS-PAGE分析诱导的重组蛋白产物。利用尿素纯化重组Cap蛋白并通过Western blotting鉴定;将纯化的重组Cap蛋白免疫新西兰大白兔制备多克隆抗体并进行Western blotting鉴定,应用间接ELISA法检测兔血清抗体效价。【结果】PCR扩增出大小为414 bp的PCV4 ORF 2基因片段,成功构建重组表达质粒pET-28a-ORF2。SDS-PAGE分析结果显示,诱导表达的重组Cap蛋白大小为16 ku;Western blotting结果表明制备的兔源多克隆抗体具有良好的免疫原性。间接ELISA检测显示,制备的兔血清抗体效价达1∶12800。【结论】本研究利用原核表达系统成功表达了PCV4 Cap蛋白并制备了多克隆抗体,试验结果为PCV4 Cap蛋白的深入研究以及后续开发PCV4基因工程亚单位疫苗、血清学诊断试剂等提供了物质基础。 展开更多
关键词 猪圆环病毒4型(PCV4) cap蛋白 原核表达 多克隆抗体
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基于北斗与CAP协议的高精度滑坡预警信息发布技术研究
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作者 黄建华 田爱国 +2 位作者 张姚 樊晶晶 官明霖 《信息产业报道》 2025年第4期0059-0061,共3页
为了将高精度滑坡预警信息实时发往灾区,降低滑坡对人员所造成的生命和财产损失,设计了一种基于北斗短报文通信方式发布高精度滑坡预警信息的方法。优化了滑坡预警信息的发布方式,将标准的北斗短报文协议和 CAP(Common Alerting Protoc... 为了将高精度滑坡预警信息实时发往灾区,降低滑坡对人员所造成的生命和财产损失,设计了一种基于北斗短报文通信方式发布高精度滑坡预警信息的方法。优化了滑坡预警信息的发布方式,将标准的北斗短报文协议和 CAP(Common Alerting Protocol)通用警报协议相结合,丰富和完善了预警信息的内容,对高精度滑坡预警信息进行了封装传输,并通过北斗终端自动回执北斗短报文预警消息,实现了基于互联网和北斗短报文通信的信息互联互通,为预警发布方式提供了应急备用手段,提高了滑坡预警信息发布的时效性和可靠性。 展开更多
关键词 滑坡 高精度 短报文协议 cap协议 预警信息发布
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表达GoAstV-2 Cap蛋白的重组FAdV-4的构建与鉴定 被引量:1
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作者 李星雨 李岩 +14 位作者 杨盼盼 刘俊杰 相梦佳 朱玉涛 邱路遥 乔麒龙 张伯顺 卜德新 韩城昊 于春梅 丛雁方 王增 李建丽 王白玉 赵军 《中国兽医学报》 北大核心 2025年第3期443-448,513,共7页
为构建表达鹅星状病毒基因2型(GoAstV-2)衣壳蛋白(Cap)的重组禽腺病毒4型(FAdV-4),本研究将GoAstV-2的Cap基因表达盒插入到含有FAdV-4感染性克隆p15A-cm-FAdV4-HNJZ中FAdV4基因组的1966 bp自然缺失区,将获得的重组感染性克隆p15A-cm-FAd... 为构建表达鹅星状病毒基因2型(GoAstV-2)衣壳蛋白(Cap)的重组禽腺病毒4型(FAdV-4),本研究将GoAstV-2的Cap基因表达盒插入到含有FAdV-4感染性克隆p15A-cm-FAdV4-HNJZ中FAdV4基因组的1966 bp自然缺失区,将获得的重组感染性克隆p15A-cm-FAdV4-HNJZ-Cap/GoAstV-2利用限制性内切酶线性化后转染鸡肝癌细胞系(chicken hepatoma cell line,LMH),拯救出表达Cap蛋白的重组病毒rFAdV4-Cap/GoAstV-2。将重组病毒在LMH细胞中连续传至第15代,提取重组病毒基因组DNA,用插入位点两侧的鉴定引物进行PCR和利用抗Cap蛋白多克隆抗体进行间接免疫荧光试验和Western blot对重组病毒进行鉴定,同时对重组病毒在LMH细胞中的复制动态进行了研究。结果显示,GoAstV-2的Cap基因能够稳定存在于重组病毒rFAdV4-Cap/GoAstV-2基因组中,而且获得稳定表达;重组病毒具有良好的体外复制能力。本研究所制备的重组病毒rFAdV4-Cap/GoAstV-2为研制防控鹅FAdV-4和GoAstV-2混合感染的新型高效二联灭活疫苗奠定了基础。 展开更多
关键词 鹅星状病毒基因2型 禽病毒4型 cap蛋白 二联灭活疫苗
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吉林省PCV3野毒株遗传进化分析及其Cap蛋白对巨噬细胞自噬的影响
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作者 闫佳慧 韩建男 +3 位作者 孙浩 李文龙 苗丽娟 刘东旭 《中国动物检疫》 2025年第2期114-119,共6页
为了探究吉林省PCV3野毒株的起源及其衣壳蛋白(Cap蛋白)是否可以引起巨噬细胞自噬,对10株吉林省PCV3野毒株(JL-1~10)全基因组序列进行遗传进化分析,并通过Western blot和qPCR检测Cap蛋白转染巨噬细胞后其自噬相关基因和蛋白表达量的变... 为了探究吉林省PCV3野毒株的起源及其衣壳蛋白(Cap蛋白)是否可以引起巨噬细胞自噬,对10株吉林省PCV3野毒株(JL-1~10)全基因组序列进行遗传进化分析,并通过Western blot和qPCR检测Cap蛋白转染巨噬细胞后其自噬相关基因和蛋白表达量的变化。结果显示:所有PCV3参考毒株与2018年在日本发现的蝙蝠圆环病毒株LC456718均在524~598 nt位置发生重组,但在JL-1~10中并未观察到该重组现象;JL-1~10与其他宿主来源圆环病毒在不同的分支中聚集;ORF1与其他圆环病毒基因组表现出明显的保守性,但ORF2没有表现出明显的保守性;JL-10和JL-5最近的共同祖先株起源时间为1978年,其他8株的共同祖先株起源时间为1979年;Cap蛋白转染巨噬细胞后,LC3-Ⅱ表达量增加,p62表达量减少;Beclin-1和LC3蛋白基因转录水平极显著升高(P<0.01),Atg7的转录水平显著升高(P<0.05)。结果表明:10株吉林省PCV3野毒株大约起源于40年前(依据参考毒株年份推断),与其他宿主来源圆环病毒的起源不同;Cap蛋白可以促进巨噬细胞自噬,引起自噬相关基因转录水平的升高。 展开更多
关键词 PCV3 进化分析 自噬 cap蛋白
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CAP1400主管道研制中大型不锈钢锻件制造技术的提升
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作者 郑建能 陈红宇 +3 位作者 刘鑫刚 周夏 李连龙 王凯 《大型铸锻件》 2025年第4期7-10,21,共5页
国内企业依托CAP1400主管道制造技术研制,包括120 t级超大型超低碳控氮奥氏体不锈钢电渣重熔技术、大型异形奥氏体不锈钢坯料锻造技术、大型厚壁承压管道弯曲成形技术、大型异形奥氏体不锈钢管道热处理变形控制及性能控制技术,形成系统... 国内企业依托CAP1400主管道制造技术研制,包括120 t级超大型超低碳控氮奥氏体不锈钢电渣重熔技术、大型异形奥氏体不锈钢坯料锻造技术、大型厚壁承压管道弯曲成形技术、大型异形奥氏体不锈钢管道热处理变形控制及性能控制技术,形成系统、稳定的大型核电锻造主管道制造技术。研究成果推广应用于多种高端不锈钢锻件制造,使我国大型不锈钢锻件制造技术得到显著提升。 展开更多
关键词 cap1400 主管道 不锈钢锻件 制造技术
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公路预防性养护中CAP抗滑封层技术的应用效果分析 被引量:1
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作者 何星星 《交通世界》 2025年第4期114-116,共3页
为有效改善旧沥青路面路用性能,提高沥青废料循环使用率,降低公路建设成本,以某高速公路项目为背景,选取局部病害路段作为试验段,对沥青还原剂(CAP)抗滑封层技术的具体应用进行综合探究,介绍了原材料及施工工艺要求,并对沥青路面CAP再... 为有效改善旧沥青路面路用性能,提高沥青废料循环使用率,降低公路建设成本,以某高速公路项目为背景,选取局部病害路段作为试验段,对沥青还原剂(CAP)抗滑封层技术的具体应用进行综合探究,介绍了原材料及施工工艺要求,并对沥青路面CAP再生沥青封层处理前后的抗滑及抗车辙性能进行分阶段检测评定。检测结果显示:①采取CAP抗滑封层处理后,沥青路面车辙指数(RDI)及抗滑指数(SRI)均有明显增大,路面抗变形及抗滑能力得到提升;②相较于CAP再生沥青封层交工阶段,道路运营2年后,沥青路面车辙指数(RDI)及抗滑指数(SRI)下降幅度较小,充分表明CAP抗滑封层处理后的沥青路面具有良好的抗滑、抗变形及耐久性能。 展开更多
关键词 预防性养护 cap 抗滑性能 抗车辙性能
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Optimizing the key parameter to accelerate the recovery of AMOC under a rapid increase of greenhouse gas forcing
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作者 Haolan Ren Fei Zheng +1 位作者 Tingwei Cao Qiang Wang 《Atmospheric and Oceanic Science Letters》 2025年第1期39-45,共7页
Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in c... Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in climate models.Previous studies have suggested that the deviation of model parameters is one of the major factors in inducing inaccurate AMOC simulations.In this work,with a low-resolution earth system model,the authors try to explore whether a reasonable adjustment of the key model parameter can help to re-establish the AMOC after its collapse.Through a new optimization strategy,the extra freshwater flux(FWF)parameter is determined to be the dominant one affecting the AMOC’s variability.The traditional ensemble optimal interpolation(EnOI)data assimilation and new machine learning methods are adopted to optimize the FWF parameter in an abrupt 4×CO_(2) forcing experiment to improve the adaptability of model parameters and accelerate the recovery of AMOC.The results show that,under an abrupt 4×CO_(2) forcing in millennial simulations,the AMOC will first collapse and then re-establish by the default FWF parameter slowly.However,during the parameter adjustment process,the saltier and colder sea water over the North Atlantic region are the dominant factors in usefully improving the adaptability of the FWF parameter and accelerating the recovery of AMOC,according to their physical relationship with FWF on the interdecadal timescale. 展开更多
关键词 Recovery of AMOC 4×CO_(2) forcing Key parameter parameter estimation Data assimilation Machine learning
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压电-光催化作用对CaP在TC4表面ZnO纳米棒阵列上的沉积机制
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作者 陈天濠 周清 许晓键 《南京航空航天大学学报(自然科学版)》 北大核心 2025年第1期139-146,共8页
由于钛合金(TC4)的表面惰性,其作为骨植入材料在生物相容性方面存在局限性。本文采用水热生长法在TC4表面成功构建了具有压电和光催化特性的ZnO纳米棒阵列涂层。在模拟体液中进行的仿生矿化实验中,通过对样品表面施加振动和紫外光照射,... 由于钛合金(TC4)的表面惰性,其作为骨植入材料在生物相容性方面存在局限性。本文采用水热生长法在TC4表面成功构建了具有压电和光催化特性的ZnO纳米棒阵列涂层。在模拟体液中进行的仿生矿化实验中,通过对样品表面施加振动和紫外光照射,探究压电-光催化共同作用下对磷酸钙(CaP)的沉积的影响,提出了一种新的仿生矿化沉积CaP的方法。结果表明:氧化锌的压电-光催化共同作用显著增强了CaP的沉积量,优于单独压电或光催化作用下的沉积量。此外,单独的振动或紫外光照也表现出较未处理样品更高的CaP沉积量。本文在钛合金片表面制备了ZnO纳米棒阵列,并提出了一种利用压电-光共同作用增强CaP沉积的方法,为涂层技术及生物医用材料的进一步开发应用提供了一种新思路。 展开更多
关键词 TC4 氧化锌 压电-光催化 磷酸钙 仿生矿化
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Shear behaviors of intermittent joints subjected to shearing cycles under constant normal stiffness conditions:Effects of loading parameters 被引量:1
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作者 Bin Wang Yujing Jiang +1 位作者 Qiangyong Zhang Hongbin Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第5期2695-2712,共18页
A conceptual model of intermittent joints is introduced to the cyclic shear test in the laboratory to explore the effects of loading parameters on its shear behavior under cyclic shear loading.The results show that th... A conceptual model of intermittent joints is introduced to the cyclic shear test in the laboratory to explore the effects of loading parameters on its shear behavior under cyclic shear loading.The results show that the loading parameters(initial normal stress,normal stiffness,and shear velocity)determine propagation paths of the wing and secondary cracks in rock bridges during the initial shear cycle,creating different morphologies of macroscopic step-path rupture surfaces and asperities on them.The differences in stress state and rupture surface induce different cyclic shear responses.It shows that high initial normal stress accelerates asperity degradation,raises shear resistance,and promotes compression of intermittent joints.In addition,high normal stiffness provides higher normal stress and shear resistance during the initial cycles and inhibits the dilation and compression of intermittent joints.High shear velocity results in a higher shear resistance,greater dilation,and greater compression.Finally,shear strength is most sensitive to initial normal stress,followed by shear velocity and normal stiffness.Moreover,average dilation angle is most sensitive to initial normal stress,followed by normal stiffness and shear velocity.During the shear cycles,frictional coefficient is affected by asperity degradation,backfilling of rock debris,and frictional area,exhibiting a non-monotonic behavior. 展开更多
关键词 Intermittent joint Cyclic shear Loading parameter Constant normal stiffness(CNS)
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Multi-objective optimization of grinding process parameters for improving gear machining precision 被引量:1
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作者 YOU Tong-fei HAN Jiang +4 位作者 TIAN Xiao-qing TANG Jian-ping LU Yi-guo LI Guang-hui XIA Lian 《Journal of Central South University》 2025年第2期538-551,共14页
The gears of new energy vehicles are required to withstand higher rotational speeds and greater loads,which puts forward higher precision essentials for gear manufacturing.However,machining process parameters can caus... The gears of new energy vehicles are required to withstand higher rotational speeds and greater loads,which puts forward higher precision essentials for gear manufacturing.However,machining process parameters can cause changes in cutting force/heat,resulting in affecting gear machining precision.Therefore,this paper studies the effect of different process parameters on gear machining precision.A multi-objective optimization model is established for the relationship between process parameters and tooth surface deviations,tooth profile deviations,and tooth lead deviations through the cutting speed,feed rate,and cutting depth of the worm wheel gear grinding machine.The response surface method(RSM)is used for experimental design,and the corresponding experimental results and optimal process parameters are obtained.Subsequently,gray relational analysis-principal component analysis(GRA-PCA),particle swarm optimization(PSO),and genetic algorithm-particle swarm optimization(GA-PSO)methods are used to analyze the experimental results and obtain different optimal process parameters.The results show that optimal process parameters obtained by the GRA-PCA,PSO,and GA-PSO methods improve the gear machining precision.Moreover,the gear machining precision obtained by GA-PSO is superior to other methods. 展开更多
关键词 worm wheel gear grinding machine gear machining precision machining process parameters multi objective optimization
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An identification model for weak influence parameters of nuclear power unit based on parameter recursion
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作者 LIANG Qian-Yun XU Xin 《四川大学学报(自然科学版)》 北大核心 2025年第4期986-991,共6页
In complex systems,there is a kind of parameters having only a minor impact on the outputs in most cases,but their accurate values are still critical for the operation of systems.In this paper,the authors focus on the... In complex systems,there is a kind of parameters having only a minor impact on the outputs in most cases,but their accurate values are still critical for the operation of systems.In this paper,the authors focus on the identification of these weak influence parameters in the complex systems and propose a identification model based on the parameter recursion.As an application,three parameters of the steam generator are identified,that is,the valve opening,the valve CV value,and the reference water level,in which the valve opening and the reference water level are weak influence parameters under most operating conditions.Numerical simulation results show that,in comparison with the multi-layer perceptron(MLP),the identification error rate is decreased.Actually,the average identification error rate for the valve opening decreases by 0.96%,for the valve CV decreases by 0.002%,and for the reference water level decreases by 12%after one recursion.After two recursions,the average identification error rate for the valve opening decreases by 11.07%,for the valve CV decreases by 2.601%,and for the reference water level decreases by 95.79%.This method can help to improve the control of the steam generator. 展开更多
关键词 Steam generator Nuclear power parameter identification Multi-layer perceptron
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MPMS-SGH:Multi-parameter Multi-step Prediction Model for Solar Greenhouse
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作者 JI Ronghua WANG Wenxuan +2 位作者 AN Dong QI Shaotian LIU Jincun 《农业机械学报》 北大核心 2025年第7期265-278,共14页
Accurately predicting environmental parameters in solar greenhouses is crucial for achieving precise environmental control.In solar greenhouses,temperature,humidity,and light intensity are crucial environmental parame... Accurately predicting environmental parameters in solar greenhouses is crucial for achieving precise environmental control.In solar greenhouses,temperature,humidity,and light intensity are crucial environmental parameters.The monitoring platform collected data on the internal environment of the solar greenhouse for one year,including temperature,humidity,and light intensity.Additionally,meteorological data,comprising outdoor temperature,outdoor humidity,and outdoor light intensity,was gathered during the same time frame.The characteristics and interrelationships among these parameters were investigated by a thorough analysis.The analysis revealed that environmental parameters in solar greenhouses displayed characteristics such as temporal variability,non-linearity,and periodicity.These parameters exhibited complex coupling relationships.Notably,these characteristics and coupling relationships exhibited pronounced seasonal variations.The multi-parameter multi-step prediction model for solar greenhouse(MPMS-SGH)was introduced,aiming to accurately predict three key greenhouse environmental parameters,and the model had certain seasonal adaptability.MPMS-SGH was structured with multiple layers,including an input layer,a preprocessing layer,a feature extraction layer,and a prediction layer.The input layer was used to generate the original sequence matrix,which included indoor temperature,indoor humidity,indoor light intensity,as well as outdoor temperature and outdoor light intensity.Then the preprocessing layer normalized,decomposed,and positionally encoded the original sequence matrix.In the feature extraction layer,the time attention mechanism and frequency attention mechanism were used to extract features from the trend component and the seasonal component,respectively.Finally,the prediction layer used a multi-layer perceptron to perform multi-step prediction of indoor environmental parameters(i.e.temperature,humidity,and light intensity).The parameter selection experiment evaluated the predictive performance of MPMS-SGH on input and output sequences of different lengths.The results indicated that with a constant output sequence length,the prediction accuracy of MPMS-SGH was firstly increased and then decreased with the increase of input sequence length.Specifically,when the input sequence length was 100,MPMS-SGH had the highest prediction accuracy,with RMSE of 0.22℃,0.28%,and 250lx for temperature,humidity,and light intensity,respectively.When the length of the input sequence remained constant,as the length of the output sequence increased,the accuracy of the model in predicting the three environmental parameters was continuously decreased.When the length of the output sequence exceeded 45,the prediction accuracy of MPMS-SGH was significantly decreased.In order to achieve the best balance between model size and performance,the input sequence length of MPMS-SGH was set to be 100,while the output sequence length was set to be 35.To assess MPMS-SGH’s performance,comparative experiments with four prediction models were conducted:SVR,STL-SVR,LSTM,and STL-LSTM.The results demonstrated that MPMS-SGH surpassed all other models,achieving RMSE of 0.15℃for temperature,0.38%for humidity,and 260lx for light intensity.Additionally,sequence decomposition can contribute to enhancing MPMS-SGH’s prediction performance.To further evaluate MPMS-SGH’s capabilities,its prediction accuracy was tested across different seasons for greenhouse environmental parameters.MPMS-SGH had the highest accuracy in predicting indoor temperature and the lowest accuracy in predicting humidity.And the accuracy of MPMS-SGH in predicting environmental parameters of the solar greenhouse fluctuated with seasons.MPMS-SGH had the highest accuracy in predicting the temperature inside the greenhouse on sunny days in spring(R^(2)=0.91),the highest accuracy in predicting the humidity inside the greenhouse on sunny days in winter(R^(2)=0.83),and the highest accuracy in predicting the light intensity inside the greenhouse on cloudy days in autumm(R^(2)=0.89).MPMS-SGH had the lowest accuracy in predicting three environmental parameters in a sunny summer greenhouse. 展开更多
关键词 solar greenhouse environmental parameter time series multi-step prediction
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Learning the parameters of a class of stochastic Lotka-Volterra systems with neural networks
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作者 WANG Zhanpeng WANG Lijin 《中国科学院大学学报(中英文)》 北大核心 2025年第1期20-25,共6页
In this paper,we propose a neural network approach to learn the parameters of a class of stochastic Lotka-Volterra systems.Approximations of the mean and covariance matrix of the observational variables are obtained f... In this paper,we propose a neural network approach to learn the parameters of a class of stochastic Lotka-Volterra systems.Approximations of the mean and covariance matrix of the observational variables are obtained from the Euler-Maruyama discretization of the underlying stochastic differential equations(SDEs),based on which the loss function is built.The stochastic gradient descent method is applied in the neural network training.Numerical experiments demonstrate the effectiveness of our method. 展开更多
关键词 stochastic Lotka-Volterra systems neural networks Euler-Maruyama scheme parameter estimation
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Influence of Process Parameters on Forming Quality of Single-Channel Multilayer by Joule Heat Fuse Additive Manufacturing
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作者 Li Suli Fan Longfei +3 位作者 Chen Jichao Gao Zhuang Xiong Jie Yang Laixia 《稀有金属材料与工程》 北大核心 2025年第5期1165-1176,共12页
To overcome the shortage of complex equipment,large volume,and high energy consumption in space capsule manufacturing,a novel sliding pressure Joule heat fuse additive manufacturing technique with reduced volume and l... To overcome the shortage of complex equipment,large volume,and high energy consumption in space capsule manufacturing,a novel sliding pressure Joule heat fuse additive manufacturing technique with reduced volume and low energy consumption was proposed.But the unreasonable process parameters may lead to the inferior consistency of the forming quality of single-channel multilayer in Joule heat additive manufacturing process,and it is difficult to reach the condition for forming thinwalled parts.Orthogonal experiments were designed to fabricate single-channel multilayer samples with varying numbers of layers,and their forming quality was evaluated.The influence of printing current,forming speed,and contact pressure on the forming quality of the single-channel multilayer was analyzed.The optimal process parameters were obtained and the quality characterization of the experiment results was conducted.Results show that the printing current has the most significant influence on the forming quality of the single-channel multilayer.Under the optimal process parameters,the forming section is well fused and the surface is continuously smooth.The surface roughness of a single-channel 3-layer sample is 0.16μm,and the average Vickers hardness of cross section fusion zone is 317 HV,which lays a foundation for the subsequent use of Joule heat additive manufacturing technique to form thinwall parts. 展开更多
关键词 Joule heat additive manufacturing single-channel multilayer process parameter forming quality
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Effect of total knee arthroplasty on the spinopelvic parameters: A systemic review and metanalysis
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作者 Mantu Jain Deb Kumar Pal +3 位作者 Rabi Narayan Sahu Bikash Ranjan Meher Binod Kumar Patro Sujit Kumar Tripathy 《World Journal of Orthopedics》 2025年第7期126-133,共8页
BACKGROUND Sagittal spinopelvic alignment(SSA)is essential for preserving a stable and effective upright posture and locomotion.Although alterations in the SSA are recognised to induce compensatory modifications in th... BACKGROUND Sagittal spinopelvic alignment(SSA)is essential for preserving a stable and effective upright posture and locomotion.Although alterations in the SSA are recognised to induce compensatory modifications in the pelvis,hips,and knees,the inverse relationship concerning knee pathology undergoing total knee arthroplasty(TKA)has been examined by a limited number of studies,yielding inconclusive results.AIM To generate evidence of the effect of TKA on the SSA from existing literature.METHODS Databases like PubMed,EMBASE,and Scopus were used to identify articles related to the“knee spine syndrome”phenomenon using a combination of subject terms and keywords such as“spinopelvic parameters”,“sagittal spinal balance”,and“total knee arthroplasty”were used with appropriate Boolean operators.Studies measuring the SSA following TKA were included,and research was conducted as per preferred reporting items for systematic review and metaanalysis guidelines.RESULTS A total of 475 participants had undergone TKA,and six studies measuring SSA were analysed.Following TKA,pelvic tilt was the only parameter that showed significant changes,while lumbar lordosis(LL),pelvic incidence,and sacral slope were non-significant,as evident from the forest plots.CONCLUSION The body's sagittal alignment is a complex balance between pelvic,spine,and lower extremity parameters.TKA,while having the potential to correct the flexion contracture,can also correct it.Still,the primary SSA for spinal pathology,i.e.,LL,may not be corrected in patients with co-existent spinal degenerative disease. 展开更多
关键词 Spinopelvic parameters Total knee arthroplasty Pelvic parameters Lower extremities parameters META-ANALYSIS
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基于广义Capped l_(2,q)范数的多项式正则化线性判别
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作者 杨源 周跃进 《哈尔滨商业大学学报(自然科学版)》 2025年第1期96-103,共8页
线性判别分析(LDA)经常存在类内散射矩阵不可逆且对异常值和噪声十分敏感等问题.为了解决这些问题,提出了一种基于广义Capped范数的多项式正则化线性判别分析(PGCLDA)方法.通过在目标函数中引入l_(2,q)范数并设置阈值,可以减少噪声的影... 线性判别分析(LDA)经常存在类内散射矩阵不可逆且对异常值和噪声十分敏感等问题.为了解决这些问题,提出了一种基于广义Capped范数的多项式正则化线性判别分析(PGCLDA)方法.通过在目标函数中引入l_(2,q)范数并设置阈值,可以减少噪声的影响、识别出数据的异常点、降低特大范数对投影方向的影响.把类间散射矩阵映射到多项式函数中增加矩阵的迹,使不同类别之间的样本距离更远,提高判别能力.对类内散射矩阵增加正则化项,避免不可逆的情形发生.在模拟和真实数据应用结果表明,本方法比其他类似的方法在减少异常值和噪声的影响与提高类别判别能力等方面更具有优越性且有更高的预测准确性. 展开更多
关键词 线性判别分析 capped l_(2 q)范数 多项式函数 散射矩阵 噪声处理 正则化
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Growth Parameters and Condition Coefficient of Three Species from Bas-Kouilou (Congo Brazzaville): Chrysichthys auratus, Geoffroy, 1809, Liza falcipinnis, Valenciennes, 1836 and Pellonula vorax, Gunther, 1868
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作者 Marcellin Mikia Anthelme Tsoumou +1 位作者 Durelle Brith Caëlle Olabi-Obath Isabelle Mady-Goma Dirat 《Open Journal of Ecology》 2025年第1期100-114,共15页
The study of the morphometric parameters of the three most abundant species in the lower course of the Kouilou River (Chrysichthys auratus, Liza falcipinnis and Pellonula vorax) was carried out. The standard length of... The study of the morphometric parameters of the three most abundant species in the lower course of the Kouilou River (Chrysichthys auratus, Liza falcipinnis and Pellonula vorax) was carried out. The standard length of Chrysichthys auratus varies between 43.57 and 210 mm, for an average of 96.70 ± 28.63 mm;the weight varies between 2.92 and 140.83 mg, an average of 73.03 ± 21.62 mg. The condition coefficient is equal to 4.42 ± 1.52. Liza falcipinnis has a standard length which varies between 59.9 mm and 158.08 mm for an average of 88.15 ± 29.74 mm;its weight varies between 4.77 and 76.21 mg, an average of 18.61 ± 11.82 mg. The condition coefficient is equal to 2.47 ± 1.57. Pellonula vorax has a standard length which varies between 60.33 mm and 117.72 mm;for an average of 80.48 ± 17.75 mm;the weight varies between 3.61 and 25.17 mg, an average of 9.03 ± 3.61 mg. The condition coefficient is equal to 2.17 ± 0.57. These three species have a minor allometric growth. 展开更多
关键词 Kouilou River Chrysichthys auratus Liza falcipinnis Pellonula vorax Growth parameters Condition Coefficient
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Improvement of Lattice Parameter Accuracy in Single Crystal XRD Based on a Laser-Induced X-Ray Source
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作者 LIU Jin WANG Qiannan LI Jiangtao 《高压物理学报》 北大核心 2025年第4期9-15,共7页
The lattice parameter,measured with sufficient accuracy,can be utilized to evaluate the quality of single crystals and to determine the equation of state for materials.We propose an iterative method for obtaining more... The lattice parameter,measured with sufficient accuracy,can be utilized to evaluate the quality of single crystals and to determine the equation of state for materials.We propose an iterative method for obtaining more precise lattice parameters using the interaction points for the pseudo-Kossel pattern obtained from laser-induced X-ray diffraction(XRD).This method has been validated by the analysis of an XRD experiment conducted on iron single crystals.Furthermore,the method was used to calculate the compression ratio and rotated angle of an LiF sample under high pressure loading.This technique provides a robust tool for in-situ characterization of structural changes in single crystals under extreme conditions.It has significant implications for studying the equation of state and phase transitions. 展开更多
关键词 lattice parameter measurement accuracy single crystal X-ray diffraction iterative algorithm high pressure ratio of compression
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Antenna Parameter Calibration for Mobile Communication Base Station via Laser Tracker
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作者 LI Junqiang CHEN Shijun +2 位作者 FENG Yujie FAN Jiancun CHEN Qiang 《ZTE Communications》 2025年第3期89-95,共7页
In the field of antenna engineering parameter calibration for indoor communication base stations,traditional methods suffer from issues such as low efficiency,poor accuracy,and limited applicability to indoor scenario... In the field of antenna engineering parameter calibration for indoor communication base stations,traditional methods suffer from issues such as low efficiency,poor accuracy,and limited applicability to indoor scenarios.To address these problems,a high-precision and high-efficiency indoor base station parameter calibration method based on laser measurement is proposed.We use a high-precision laser tracker to measure and determine the coordinate system transformation relationship,and further obtain the coordinates and attitude of the base station.In addition,we propose a simple calibration method based on point cloud fitting for specific scenes.Simulation results show that using common commercial laser trackers,we can achieve a coordinate correction accuracy of 1 cm and an angle correction accuracy of 0.25°,which is sufficient to meet the needs of wireless positioning. 展开更多
关键词 antenna parameter parameter measurement coordinate transformation point cloud laser track angle correction
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Influence of Processed Mustard Seed Meals on Growth and Health Parameters in Indian Major Carps
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作者 Shivanshu Garg Himanshu Punetha +8 位作者 Anant Deogaonkar Deepshree Kumar Ashutosh Mishra AshokKumar Verma Nityanand Pandey Ranjeet Singh Saurabh Gangola Sunil Kumar Amit Mittal 《Research in Ecology》 2025年第2期93-105,共13页
The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and wh... The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and white mustard meals as fish feed ingredients for Indian Major Carps.Fish were fed with 10%mustard meal-supplemented diets in three forms:Raw(R),Anti-nutritional Rich(AR),and Anti-nutritional Lowered(AL),alongside a control group using floating feed.The three-month indoor experiment(September-November 2023)was conducted in FRP tanks with triplicate treatments.Blood analysis revealed compromised health in AR-fed carps,with reduced hemoglobin levels in rohu,catla and mrigal and elevated total leukocyte counts indicating inflammation in all the three carps studied here.Liver function was impaired in AR-fed fish,shown by increased alanine transaminase levels,highest in rohu followed by mrigal and catla.Histopathological examination of AR-fed carps liver tissue revealed necrotic spots,deformed hepatocytes,and significant vacuolation.In contrast,AL-fed fish demonstrated improved health parameters through Complete Blood Count analysis,liver function tests,and histo-pathological observations,suggesting successful reduction of anti-nutritional factors in the processed mustard meals.In near future,replacement of unprocessed seed meal with processed seed meal will lead to economic gains in fish farming. 展开更多
关键词 Biochemical parameters GLUCOSINOLATES Growth parameters Histo-Pathological Analysis Indian Major Carps Liver Function Test
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