The aim of the study was to determine the feasibility of the Vitellaria paradoxa nutshell as a new medicinal resource for treating diabetes. A total of forty-one compounds were identified by HPLC-DAD-Q-TOF-MS and phyt...The aim of the study was to determine the feasibility of the Vitellaria paradoxa nutshell as a new medicinal resource for treating diabetes. A total of forty-one compounds were identified by HPLC-DAD-Q-TOF-MS and phytochemical methods in V. paradoxa nutshell methanol extract. Based on HPLC fingerprints, four characteristic constituents were quantified and the origin of twenty-eight V. paradoxa nutshells from seven sub-Saharan countries was compared, which were classified into three groups with chemometric method. Twenty-eight samples contained high total phenolic content, and exhibited moderate-higher antioxidant activity and strong α-glucosidase inhibitory activity. Furthermore, all fractions and isolated compounds were evaluated for their antioxidant and α-glucosidase inhibitory activities, and α-glucosidase inhibitory action mechanism of four characteristic constituents including protocatechuic acid, 3, 5, 7-trihydroxycoumarin,(2 R, 3 R)-(+)-taxifolin and quercetin was investigated via molecular docking method, which were all stabilized by hydrogen bonds with α-glucosidase. The study provided an effective approach to waste utilization of V. paradoxa nutshell, which would help to resolve waste environmental pollution and provide a basis for developing potential herbal resource for treating diabetes.展开更多
Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, ham...Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, hampered by the poor rooting success of its planting material with adverse consequences on field establishment and total fruit yield. A 3 × 2 factorial experiment arranged in a randomized complete block design was carried out in 2012 at Bole in the Interior Savanna agro-ecological zone. The objective was to assess the rooting success of Shea shoots by the air-layering technique using two media types (palm fibre and Sphagnum moss) and three IBA hormone concentrations (0, 5000 and 10,000 ppm) under contrasting climatic (wet and dry) conditions. Layered shoots which were sprayed with 10,000 ppm IBA and wrapped with Sphagnum moss gave significantly (P < 0.05) higher rooting success in terms of more roots per cutting (73.3%) and longer roots per cutting (9.0 cm) than palm fibre at both 5000 ppm (30.0%;3.7 cm) and 10,000 ppm (46.7%;7.9 cm) concentrations. Higher rooting success was significantly achieved in the wet season than in the dry season. Sphagnum moss treated with 10,000 ppm IBA facilitated the translocation of higher levels of sugar and total free phenol (TFP) to the base of the layered shoots which resulted in significantly (P < 0.05) higher number of roots and better roots protection from fungal infection than the other treatments. Rooting of layered shoots was affected to a greater extent by low temperatures (Rooting = 836 – 34.2 Temp (low);R2 = 82%, p < 0.001) than by high temperatures (Rooting = 5250 – 175.0 Temp (high);R2 = 64.5%, p < 0.009). Rooting of layered shoots was also significantly and negatively affected by the spread of the canopy of the selected tree such that closed canopy trees resulted in higher and better rooting that open canopy trees (Rooting = 113.87-23.697(Canopy spread);R2 = 89%, p = 0.002;n = 9). Furthermore, significant and positive correlations were found between IBA concentration level and simple sugars (r = 0.99;P < 0.0001;n = 9) and also between IBA concentration level and total free phenols (r = 0.98;P < 0.0002;n = 9). The study concluded that to achieve high rooting success in the propagation of Shea nut tree by the air-layering technique, a combination of Sphagnum moss treated with 10,000 ppm of IBA hormone should be used and the whole set up carried out in the wet season.展开更多
[Objective]The paper was to reveal the preparation and regeneration conditions of Ceratocystis caradoxa protoplast.[Method]Using multi-factors orthogonal test,the effects of hypha age,enzyme system,time of hydrolysis,...[Objective]The paper was to reveal the preparation and regeneration conditions of Ceratocystis caradoxa protoplast.[Method]Using multi-factors orthogonal test,the effects of hypha age,enzyme system,time of hydrolysis,hydrolysis temperature,osmotic pressure stabilizer and regeneration medium on C.paradoxa protoplast were studied.[Result]The optimum condition for preparing protoplast were conidia cultured in liguid SYM medium for 24 h,enzyme mixture of 1%driselase and 1%lytic enzyme used to digest hypha for 1.5 h,0.7 mol/L MgSO4·7H2O used as osmotic stabilizer,or individual enzyme 1%driselase used to digest hypha for 1.5 h,1.0 mol/L mannitol as osmotic stabilizer.The regeneration rate was over 40%under C.paradoxa protoplast regenerating on CM medium with mannitol and hydrolysis for 2 h.[Conclusion]Higher protoplast yield and higher regeneration rate could be obtained under above conditions,which is beneficial for transformation and further research.展开更多
To find possible therapeutic applications involving the Central Nervous System (CNS) for herbals is a major challenge during functional food and drug discovery and development programmes. Despite the availability of n...To find possible therapeutic applications involving the Central Nervous System (CNS) for herbals is a major challenge during functional food and drug discovery and development programmes. Despite the availability of numerous in vitro and in vivo tests, there is no single agreed screening procedure for pharmacological testing of herbal extracts with anticipated CNS activity. Experience gained from more than 25 years of testing has shown that two models give reasonably reliable orientation for future CNS applications: construction of an electropharmacogram based on wireless recording of field potentials from the depth of the brain of freely moving rats (Tele-Stereo-EEG) and recording of the population spike produced by pyramidal cells from hippocampal slices in vitro. A combination of these two methods has now been used to characterize the pharmacological profile of extracts from Rhodiola rosea root, Oenothera paradoxa seeds and Paullinia cupana seeds. Spectral analysis of field potentials revealed attenuation of alpha2 and beta1 waves was common for all extracts. According to previous studies, this is interpreted as activation of the dopaminergic and glutamatergic transmission. In addition, Oenothera and Rhodiola extracts attenuated delta and theta power, probably related to interference with the cholinergic and norepinephrinergic transmission, respectively. Using discriminant analysis for comparison with reference pharmaceutical and botanical drugs, Rhodiola projected near the position of Ginkgo extract, whereas Oenothera extract was projected near the position of Tramadol, an analgesic drug. Physical motion was increased only in the presence of Paullinia extract and caffeine. Increases of long-term potentiation were observed in the presence of Rhodiola extract, Paullinia extract and caffeine. The combined information predicts stimulant and cognitive function-enhancing activities in humans for the Rhodiola extract, which could also be used as a possible caffeine-replacement, and antidepressant and analgesic activity for the Oenothera extract.展开更多
This research was carried out at two locations, Ada and Aveglo at the Volta Estuary, Ghana to evaluate the importance of sediments as regulatory media in controlling the levels of four metals, Manganese (Mn), Zinc (Zn...This research was carried out at two locations, Ada and Aveglo at the Volta Estuary, Ghana to evaluate the importance of sediments as regulatory media in controlling the levels of four metals, Manganese (Mn), Zinc (Zn), Iron (Fe) and Mercury (Hg) in the tissues of the clam, Galatea paradoxa (Born 1778) and to investigate the possible relationships between the concentrations of the metals in the sediments and the clams. The clams were categorized into three size classes as follows: small (25 - 40 mm), medium (41 - 55 mm), and large (above 55 mm). To understand the possible relationships between the concentrations of the studied metals in the sediments and in the tissues of the three clam size classes, the monthly concentrations of the studied metals were graphed and subjected to Pearson correlation analyses (p < 0.05) to identify metal accumulation patterns and determine whether or not positive relationship patterns existed between the concentrations in the clams and sediment samples. The correlation revealed no simple linear relationships between the concentrations of four heavy metals in the clam tissues and the sediments at the two sampling stations although some distinct trends were observed. Mn concentrations in the clams and sediments from the two stations showed some clear positive relationship patterns with some increments in monthly sediment concentrations resulting in increments in clam tissue concentrations. This relationship though, was not too clear-cut.展开更多
Shea butter (Vitellaria paradoxa) tree is one of the most important tree species in Africa owing to its high potential in reduction of poverty and hunger in rural areas and enhancing environmental sustainability. All ...Shea butter (Vitellaria paradoxa) tree is one of the most important tree species in Africa owing to its high potential in reduction of poverty and hunger in rural areas and enhancing environmental sustainability. All parts of shea tree are important from bark to wood and from leaves to fruits. Despite all the uses and many more, shea is amongst the endangered species of trees because of over-utilization, low regeneration abilities and lack of efficient propagation methods. Consequently, this research seeks to determine the most efficient methods for propagating V. paradoxa. The experiment consists of three stages. In the first stage, complete fruits were planted without depulping;in the second experiment, the seeds were exposed to single seed treatments that were applied to the seeds and in the third experiment, the seeds were exposed to double seed treatments. It was observed that intact fruits did not germinate while the results showed the single seeds treatment to significantly have affected germination performance. De-shelled seeds germinated faster (43 days) than all other treatments but 48 hours soaking produced the highest germination percentage (91.7%). An even better result was recorded with double seeds treatments in which de-shelled plus 24 hours soaked seed germinated faster (39.3 days) than other treatments while sandpapered plus 24 hours soaked seeds had the best germination percentage (75%). Therefore, scarification using sandpaper plus soaking for 24 hours was identified as the best treatment for breaking seed dormancy in V. paradoxa.展开更多
The housing sector today uses elaborate materials such as cement,iron,sand,often prohibitively expensive and whose production generates a strong environmental impact(scarcity of resources,transport,greenhouse gas gree...The housing sector today uses elaborate materials such as cement,iron,sand,often prohibitively expensive and whose production generates a strong environmental impact(scarcity of resources,transport,greenhouse gas greenhouse,etc.).In order to meet the challenges of sustainable development,earth construction is experiencing a resurgence of interest these days.Despite its many advantages,raw earth material has drawbacks,in particular its low mechanical resistance and its loss of geometric characteristics in the face of water,which slow down its development.As part of this study,the mechanical characteristics and durability of raw earth were improved by using residual water from the processing of Parkia Biglobosa(nere)and Vitellaria Paradoxa(shea)nuts in order to optimize its use for the construction of modern buildings.To this end,the decoctions resulting from the artisanal transformation of the nut of the Parkia Biglobosa into African mustard and of the Vitellaria Paradoxa into shea butter were added to the raw earth according to volume proportions of 25%,50%,75%and 100%of the aqueous solution to obtain the projected composites.Thus,mechanical characterization and durability tests were carried out on the composites obtained.The results revealed that the decoctions of Vitellaria Paradoxa and Parkia Biglobosa improve the compressive strength of the material by up to 90%and 260%,respectively.Furthermore,these decoctions improved the resistance to water penetration of the 100%additive composite by 1.5 times for Vitellaria Paradoxa and 5 times for Parkia Biglobosa.This study shows that it is possible to use decoctions as raw earth stabilizers to build modern,ecological buildings at lower energy costs.However,more in-depth studies on surface wettability and long-term durability are planned to better characterize the geomaterial.展开更多
为了解乳油木叶绿体基因组密码子的使用模式,该研究以从乳油木叶绿体基因组中筛选得到53条长度大于300 bp且非重复的CDS(Coding DNA sequence)序列为材料,利用Codon W 1.4.2和在线软件CUSP分析其密码子偏好性。结果显示,密码子第3位GC...为了解乳油木叶绿体基因组密码子的使用模式,该研究以从乳油木叶绿体基因组中筛选得到53条长度大于300 bp且非重复的CDS(Coding DNA sequence)序列为材料,利用Codon W 1.4.2和在线软件CUSP分析其密码子偏好性。结果显示,密码子第3位GC含量为27.47%,ENC范围在36.31~56.88,平均值为42.98。RSCU值大于1的密码子有28个,其中27个以A或U为结尾。中性绘图分析、ENC-plot分析和PR2-plot分析的结果显示乳油木叶绿体基因密码子偏好性主要受突变的影响。最优密码子为GGA、GAU、CAU和UUG。本研究结果为提高异源基因表达水平、改良乳油木重要经济性状等提供参考依据。展开更多
基金supported by the General Program Foundation of Jiangsu Food and Pharmaceutical Science College(No.JSFP·2018005-Z)the Natural Science Foundation of Jiangsu Province(No.BK20170742)the Youth Science Fund Project of National Natural Science Foundation of China(No.81703383)
文摘The aim of the study was to determine the feasibility of the Vitellaria paradoxa nutshell as a new medicinal resource for treating diabetes. A total of forty-one compounds were identified by HPLC-DAD-Q-TOF-MS and phytochemical methods in V. paradoxa nutshell methanol extract. Based on HPLC fingerprints, four characteristic constituents were quantified and the origin of twenty-eight V. paradoxa nutshells from seven sub-Saharan countries was compared, which were classified into three groups with chemometric method. Twenty-eight samples contained high total phenolic content, and exhibited moderate-higher antioxidant activity and strong α-glucosidase inhibitory activity. Furthermore, all fractions and isolated compounds were evaluated for their antioxidant and α-glucosidase inhibitory activities, and α-glucosidase inhibitory action mechanism of four characteristic constituents including protocatechuic acid, 3, 5, 7-trihydroxycoumarin,(2 R, 3 R)-(+)-taxifolin and quercetin was investigated via molecular docking method, which were all stabilized by hydrogen bonds with α-glucosidase. The study provided an effective approach to waste utilization of V. paradoxa nutshell, which would help to resolve waste environmental pollution and provide a basis for developing potential herbal resource for treating diabetes.
文摘Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, hampered by the poor rooting success of its planting material with adverse consequences on field establishment and total fruit yield. A 3 × 2 factorial experiment arranged in a randomized complete block design was carried out in 2012 at Bole in the Interior Savanna agro-ecological zone. The objective was to assess the rooting success of Shea shoots by the air-layering technique using two media types (palm fibre and Sphagnum moss) and three IBA hormone concentrations (0, 5000 and 10,000 ppm) under contrasting climatic (wet and dry) conditions. Layered shoots which were sprayed with 10,000 ppm IBA and wrapped with Sphagnum moss gave significantly (P < 0.05) higher rooting success in terms of more roots per cutting (73.3%) and longer roots per cutting (9.0 cm) than palm fibre at both 5000 ppm (30.0%;3.7 cm) and 10,000 ppm (46.7%;7.9 cm) concentrations. Higher rooting success was significantly achieved in the wet season than in the dry season. Sphagnum moss treated with 10,000 ppm IBA facilitated the translocation of higher levels of sugar and total free phenol (TFP) to the base of the layered shoots which resulted in significantly (P < 0.05) higher number of roots and better roots protection from fungal infection than the other treatments. Rooting of layered shoots was affected to a greater extent by low temperatures (Rooting = 836 – 34.2 Temp (low);R2 = 82%, p < 0.001) than by high temperatures (Rooting = 5250 – 175.0 Temp (high);R2 = 64.5%, p < 0.009). Rooting of layered shoots was also significantly and negatively affected by the spread of the canopy of the selected tree such that closed canopy trees resulted in higher and better rooting that open canopy trees (Rooting = 113.87-23.697(Canopy spread);R2 = 89%, p = 0.002;n = 9). Furthermore, significant and positive correlations were found between IBA concentration level and simple sugars (r = 0.99;P < 0.0001;n = 9) and also between IBA concentration level and total free phenols (r = 0.98;P < 0.0002;n = 9). The study concluded that to achieve high rooting success in the propagation of Shea nut tree by the air-layering technique, a combination of Sphagnum moss treated with 10,000 ppm of IBA hormone should be used and the whole set up carried out in the wet season.
基金Supported by Key Project of Hainan Province(ZDYF2019072)Basic Scientific Research Project of CATAS(16301520190010).
文摘[Objective]The paper was to reveal the preparation and regeneration conditions of Ceratocystis caradoxa protoplast.[Method]Using multi-factors orthogonal test,the effects of hypha age,enzyme system,time of hydrolysis,hydrolysis temperature,osmotic pressure stabilizer and regeneration medium on C.paradoxa protoplast were studied.[Result]The optimum condition for preparing protoplast were conidia cultured in liguid SYM medium for 24 h,enzyme mixture of 1%driselase and 1%lytic enzyme used to digest hypha for 1.5 h,0.7 mol/L MgSO4·7H2O used as osmotic stabilizer,or individual enzyme 1%driselase used to digest hypha for 1.5 h,1.0 mol/L mannitol as osmotic stabilizer.The regeneration rate was over 40%under C.paradoxa protoplast regenerating on CM medium with mannitol and hydrolysis for 2 h.[Conclusion]Higher protoplast yield and higher regeneration rate could be obtained under above conditions,which is beneficial for transformation and further research.
文摘To find possible therapeutic applications involving the Central Nervous System (CNS) for herbals is a major challenge during functional food and drug discovery and development programmes. Despite the availability of numerous in vitro and in vivo tests, there is no single agreed screening procedure for pharmacological testing of herbal extracts with anticipated CNS activity. Experience gained from more than 25 years of testing has shown that two models give reasonably reliable orientation for future CNS applications: construction of an electropharmacogram based on wireless recording of field potentials from the depth of the brain of freely moving rats (Tele-Stereo-EEG) and recording of the population spike produced by pyramidal cells from hippocampal slices in vitro. A combination of these two methods has now been used to characterize the pharmacological profile of extracts from Rhodiola rosea root, Oenothera paradoxa seeds and Paullinia cupana seeds. Spectral analysis of field potentials revealed attenuation of alpha2 and beta1 waves was common for all extracts. According to previous studies, this is interpreted as activation of the dopaminergic and glutamatergic transmission. In addition, Oenothera and Rhodiola extracts attenuated delta and theta power, probably related to interference with the cholinergic and norepinephrinergic transmission, respectively. Using discriminant analysis for comparison with reference pharmaceutical and botanical drugs, Rhodiola projected near the position of Ginkgo extract, whereas Oenothera extract was projected near the position of Tramadol, an analgesic drug. Physical motion was increased only in the presence of Paullinia extract and caffeine. Increases of long-term potentiation were observed in the presence of Rhodiola extract, Paullinia extract and caffeine. The combined information predicts stimulant and cognitive function-enhancing activities in humans for the Rhodiola extract, which could also be used as a possible caffeine-replacement, and antidepressant and analgesic activity for the Oenothera extract.
文摘This research was carried out at two locations, Ada and Aveglo at the Volta Estuary, Ghana to evaluate the importance of sediments as regulatory media in controlling the levels of four metals, Manganese (Mn), Zinc (Zn), Iron (Fe) and Mercury (Hg) in the tissues of the clam, Galatea paradoxa (Born 1778) and to investigate the possible relationships between the concentrations of the metals in the sediments and the clams. The clams were categorized into three size classes as follows: small (25 - 40 mm), medium (41 - 55 mm), and large (above 55 mm). To understand the possible relationships between the concentrations of the studied metals in the sediments and in the tissues of the three clam size classes, the monthly concentrations of the studied metals were graphed and subjected to Pearson correlation analyses (p < 0.05) to identify metal accumulation patterns and determine whether or not positive relationship patterns existed between the concentrations in the clams and sediment samples. The correlation revealed no simple linear relationships between the concentrations of four heavy metals in the clam tissues and the sediments at the two sampling stations although some distinct trends were observed. Mn concentrations in the clams and sediments from the two stations showed some clear positive relationship patterns with some increments in monthly sediment concentrations resulting in increments in clam tissue concentrations. This relationship though, was not too clear-cut.
文摘Shea butter (Vitellaria paradoxa) tree is one of the most important tree species in Africa owing to its high potential in reduction of poverty and hunger in rural areas and enhancing environmental sustainability. All parts of shea tree are important from bark to wood and from leaves to fruits. Despite all the uses and many more, shea is amongst the endangered species of trees because of over-utilization, low regeneration abilities and lack of efficient propagation methods. Consequently, this research seeks to determine the most efficient methods for propagating V. paradoxa. The experiment consists of three stages. In the first stage, complete fruits were planted without depulping;in the second experiment, the seeds were exposed to single seed treatments that were applied to the seeds and in the third experiment, the seeds were exposed to double seed treatments. It was observed that intact fruits did not germinate while the results showed the single seeds treatment to significantly have affected germination performance. De-shelled seeds germinated faster (43 days) than all other treatments but 48 hours soaking produced the highest germination percentage (91.7%). An even better result was recorded with double seeds treatments in which de-shelled plus 24 hours soaked seed germinated faster (39.3 days) than other treatments while sandpapered plus 24 hours soaked seeds had the best germination percentage (75%). Therefore, scarification using sandpaper plus soaking for 24 hours was identified as the best treatment for breaking seed dormancy in V. paradoxa.
基金supported by the Beninese state.An author received a trainer training grant from the Benin state for stays in the Jean Lamour Institute Laboratory of the University of Lorraine in France to carry out tests.
文摘The housing sector today uses elaborate materials such as cement,iron,sand,often prohibitively expensive and whose production generates a strong environmental impact(scarcity of resources,transport,greenhouse gas greenhouse,etc.).In order to meet the challenges of sustainable development,earth construction is experiencing a resurgence of interest these days.Despite its many advantages,raw earth material has drawbacks,in particular its low mechanical resistance and its loss of geometric characteristics in the face of water,which slow down its development.As part of this study,the mechanical characteristics and durability of raw earth were improved by using residual water from the processing of Parkia Biglobosa(nere)and Vitellaria Paradoxa(shea)nuts in order to optimize its use for the construction of modern buildings.To this end,the decoctions resulting from the artisanal transformation of the nut of the Parkia Biglobosa into African mustard and of the Vitellaria Paradoxa into shea butter were added to the raw earth according to volume proportions of 25%,50%,75%and 100%of the aqueous solution to obtain the projected composites.Thus,mechanical characterization and durability tests were carried out on the composites obtained.The results revealed that the decoctions of Vitellaria Paradoxa and Parkia Biglobosa improve the compressive strength of the material by up to 90%and 260%,respectively.Furthermore,these decoctions improved the resistance to water penetration of the 100%additive composite by 1.5 times for Vitellaria Paradoxa and 5 times for Parkia Biglobosa.This study shows that it is possible to use decoctions as raw earth stabilizers to build modern,ecological buildings at lower energy costs.However,more in-depth studies on surface wettability and long-term durability are planned to better characterize the geomaterial.
文摘为了解乳油木叶绿体基因组密码子的使用模式,该研究以从乳油木叶绿体基因组中筛选得到53条长度大于300 bp且非重复的CDS(Coding DNA sequence)序列为材料,利用Codon W 1.4.2和在线软件CUSP分析其密码子偏好性。结果显示,密码子第3位GC含量为27.47%,ENC范围在36.31~56.88,平均值为42.98。RSCU值大于1的密码子有28个,其中27个以A或U为结尾。中性绘图分析、ENC-plot分析和PR2-plot分析的结果显示乳油木叶绿体基因密码子偏好性主要受突变的影响。最优密码子为GGA、GAU、CAU和UUG。本研究结果为提高异源基因表达水平、改良乳油木重要经济性状等提供参考依据。
