[目的]通过对5个石榴品种的蛋白质的聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis,PAGE)分析,为石榴种质资源的鉴定提供科学依据。[方法]以5个石榴品种(大红袍、冰糖籽、大青皮、软籽石榴、谢花甜)为试验材料,分别提取其总...[目的]通过对5个石榴品种的蛋白质的聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis,PAGE)分析,为石榴种质资源的鉴定提供科学依据。[方法]以5个石榴品种(大红袍、冰糖籽、大青皮、软籽石榴、谢花甜)为试验材料,分别提取其总蛋白、盐溶蛋白、水溶蛋白,并进行PAGE电泳检测。[结果]蛋白质的PAGE电泳具有条带的多样性,不仅能很好的鉴定区分不同品种,还能通过相似度指数进行聚类分析,探究品种间的亲缘关系。石榴的总蛋白与盐溶蛋白电泳分析结果基本一致,大青皮与谢花甜,软籽石榴与冰糖籽亲缘关系较近;其水溶蛋白聚类分析中,5个品种间无明显分组聚合现象。[结论]该研究结果表明PAGE技术能较好的揭示品种间的遗传变异,可用于分析石榴种质的遗传多样性和亲缘关系,其结果为石榴种质资源的保存和良种培育提供了科学依据。展开更多
Genetic diversity in 30 genotypes of Pumpkins <span style="font-family:Verdana;">was</span><span style="font-family:Verdana;"> collected from unexplored mountainous areas of Khybe...Genetic diversity in 30 genotypes of Pumpkins <span style="font-family:Verdana;">was</span><span style="font-family:Verdana;"> collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00</span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity;to broaden the gene pool. Further collection of the important germplasm is needed </span><span style="font-family:Verdana;">to be </span><span style="font-family:Verdana;">used in the development of improved cultivars with respect to quality and quantity.</span>展开更多
A web page clustering algorithm called PageCluster and the improved algorithm ImPageCluster solving overlapping are proposed. These methods not only take the web structure and page hyperlink into account, but also con...A web page clustering algorithm called PageCluster and the improved algorithm ImPageCluster solving overlapping are proposed. These methods not only take the web structure and page hyperlink into account, but also consider the importance of each page which is described as in-weight and out-weight. Compared with the traditional clustering methods, the experiments show that the runtimes of the proposed algorithms are less with the improved accuracies.展开更多
利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明，油用型红花醇溶蛋白位点存在丰富的变异类型，共分离出15条迁移率不同的谱带，每份材料具有3—12条...利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明，油用型红花醇溶蛋白位点存在丰富的变异类型，共分离出15条迁移率不同的谱带，每份材料具有3—12条不等，平均8．5条。材料间平均遗传相似系数（GS）为0．5303，变幅为0．1333—1．000，且各洲间遗传多样性大于洲内遗传多样性。在GS值为0．512的水平上，供试材料聚为六大类，聚类结果表明，红花醇溶蛋白图谱类型与其地理分布有一定的相关，但不显著。展开更多
文摘[目的]通过对5个石榴品种的蛋白质的聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis,PAGE)分析,为石榴种质资源的鉴定提供科学依据。[方法]以5个石榴品种(大红袍、冰糖籽、大青皮、软籽石榴、谢花甜)为试验材料,分别提取其总蛋白、盐溶蛋白、水溶蛋白,并进行PAGE电泳检测。[结果]蛋白质的PAGE电泳具有条带的多样性,不仅能很好的鉴定区分不同品种,还能通过相似度指数进行聚类分析,探究品种间的亲缘关系。石榴的总蛋白与盐溶蛋白电泳分析结果基本一致,大青皮与谢花甜,软籽石榴与冰糖籽亲缘关系较近;其水溶蛋白聚类分析中,5个品种间无明显分组聚合现象。[结论]该研究结果表明PAGE技术能较好的揭示品种间的遗传变异,可用于分析石榴种质的遗传多样性和亲缘关系,其结果为石榴种质资源的保存和良种培育提供了科学依据。
文摘Genetic diversity in 30 genotypes of Pumpkins <span style="font-family:Verdana;">was</span><span style="font-family:Verdana;"> collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00</span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity;to broaden the gene pool. Further collection of the important germplasm is needed </span><span style="font-family:Verdana;">to be </span><span style="font-family:Verdana;">used in the development of improved cultivars with respect to quality and quantity.</span>
基金Sponsored bythe Huo Ying-Dong Education Foundation of China(91101)
文摘A web page clustering algorithm called PageCluster and the improved algorithm ImPageCluster solving overlapping are proposed. These methods not only take the web structure and page hyperlink into account, but also consider the importance of each page which is described as in-weight and out-weight. Compared with the traditional clustering methods, the experiments show that the runtimes of the proposed algorithms are less with the improved accuracies.
文摘利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明，油用型红花醇溶蛋白位点存在丰富的变异类型，共分离出15条迁移率不同的谱带，每份材料具有3—12条不等，平均8．5条。材料间平均遗传相似系数（GS）为0．5303，变幅为0．1333—1．000，且各洲间遗传多样性大于洲内遗传多样性。在GS值为0．512的水平上，供试材料聚为六大类，聚类结果表明，红花醇溶蛋白图谱类型与其地理分布有一定的相关，但不显著。
