p16/Ki-67双重染色检测(双染)作为一种相对客观的检测方法,已在多个国家获批应用于临床。本共识基于循证医学证据,对双染样本的取材与制备、检测流程、结果判读及报告规范达成共识,并推荐双染可用于高危人乳头瘤病毒(high-risk human pa...p16/Ki-67双重染色检测(双染)作为一种相对客观的检测方法,已在多个国家获批应用于临床。本共识基于循证医学证据,对双染样本的取材与制备、检测流程、结果判读及报告规范达成共识,并推荐双染可用于高危人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)初筛不分型阳性或部分分型其他12种(HR 12)阳性人群的分流;也可用于联合筛查时HR-HPV不分型阳性或部分分型HR 12阳性,且细胞学正常或轻度异常时的人群分流;还可用于筛查结果异常(HR-HPV检测或HR-HPV联合细胞学检测)后随访人群的分流。通过本共识的制定,旨在规范双染检测技术的临床应用,实施子宫颈癌精准筛查。展开更多
p16/Ki-67双重染色检测(双染)作为一种相对客观的检测方法,已在多个国家获批应用于临床。本共识基于循证医学证据,对双染样本的取材与制备、检测流程、结果判读及报告规范达成共识,并推荐双染可用于高危人乳头瘤病毒(high-risk human pa...p16/Ki-67双重染色检测(双染)作为一种相对客观的检测方法,已在多个国家获批应用于临床。本共识基于循证医学证据,对双染样本的取材与制备、检测流程、结果判读及报告规范达成共识,并推荐双染可用于高危人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)初筛不分型阳性或部分分型其他12种(HR 12)阳性人群的分流;也可用于联合筛查时HR-HPV不分型阳性或部分分型HR 12阳性,且细胞学正常或轻度异常时的人群分流;还可用于筛查结果异常(HR-HPV检测或HR-HPV联合细胞学检测)后随访人群的分流。通过本共识的制定,旨在规范双染检测技术的临床应用,实施子宫颈癌精准筛查。展开更多
BACKGROUND The high prevalence of human papillomavirus(HPV)infection in oropharyngeal squamous cell carcinoma(SCC)is well established,and p16 expression is a strong predictor.HPV-related tumors exhibit unique mechanis...BACKGROUND The high prevalence of human papillomavirus(HPV)infection in oropharyngeal squamous cell carcinoma(SCC)is well established,and p16 expression is a strong predictor.HPV-related tumors exhibit unique mechanisms that target p16 and p53 proteins.However,research on HPV prevalence and the combined predictive value of p16 and p53 expression in head and neck cutaneous SCC(HNCSCC),particularly in Asian populations,remains limited.This retrospective study surveyed 62 patients with HNSCC(2011-2020),excluding those with facial warts or other skin cancer.AIM To explore the prevalence of HPV and the predictive value of p16 and p53 expression in HNCSCC in Asian populations.METHODS All patients underwent wide excision and biopsy.Immunohistochemical staining for HPV,p16,and p53 yielded positive and negative results.The relevance of each marker was investigated by categorizing the tumor locations into high-risk and middle-risk zones based on recurrence frequency.RESULTS Of the 62 patients,20(32.26%)were male,with an average age of 82.27 years(range 26-103 years).High-risk included 19 cases(30.65%),with the eyelid and lip being the most common sites(five cases,8.06%).Middle-risk included 43 cases(69.35%),with the cheek being the most common(29 cases,46.77%).The p16 expression was detected in 24 patients(38.71%),p53 expression in 42 patients(72.58%),and HPV in five patients(8.06%).No significant association was found between p16 expression and the presence of HPV(P>0.99),with a positive predictive value of 8.33%.CONCLUSION This study revealed that p16,a surrogate HPV marker in oropharyngeal SCC,is not reliable in HNCSCC,providing valuable insights for further research in Asian populations.展开更多
文摘目的探讨RUNX3、P16和DAPK基因启动子DNA甲基化与非小细胞肺癌(non-small cell lung cancer,NSCLC)化疗敏感性的关系。方法选取2023年2月至2025年2月于株洲市中心医院接受铂类化疗的初治晚期NSCLC患者116例为研究对象,根据化疗疗效将其分为敏感组(n=81)和耐受组(n=35)。比较两组患者的病灶组织标本RUNX3、P16、DAPK基因启动子DNA甲基化情况、基因转录水平绝对定量,采用受试者操作特征曲线(receiver operating characteristic curve,ROC曲线)评估RUNX3、P16、DAPK基因表达水平对铂类化疗耐药的预测价值。结果耐受组患者肺癌组织的RUNX3、P16和DAPK基因启动子DNA甲基化比例均显著高于敏感组(P<0.05)。耐受组患者肺癌组织的RUNX3、P16和DAPK基因转录水平均显著低于敏感组(P<0.05)。ROC曲线显示,肺癌组织RUNX3、P16、DAPK基因转录水平预测NSCLC患者化疗敏感的最佳截断值分别为2.93×10^(6)、5.22×10^(4)、6.420×10^(5),曲线下面积(area under the curve,AUC)分别为0.726、0.748、0.763。三者联合预测NSCLC患者化疗敏感的AUC为0.883,敏感度为81.82%、特异性为78.05%。结论RUNX3、P16、DAPK基因甲基化可能是晚期NSCLC患者铂类化疗耐药的原因之一,早期测定此类基因转录水平对预估化疗耐药、制定个体化治疗方案具有积极作用。
文摘p16/Ki-67双重染色检测(双染)作为一种相对客观的检测方法,已在多个国家获批应用于临床。本共识基于循证医学证据,对双染样本的取材与制备、检测流程、结果判读及报告规范达成共识,并推荐双染可用于高危人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)初筛不分型阳性或部分分型其他12种(HR 12)阳性人群的分流;也可用于联合筛查时HR-HPV不分型阳性或部分分型HR 12阳性,且细胞学正常或轻度异常时的人群分流;还可用于筛查结果异常(HR-HPV检测或HR-HPV联合细胞学检测)后随访人群的分流。通过本共识的制定,旨在规范双染检测技术的临床应用,实施子宫颈癌精准筛查。
文摘p16/Ki-67双重染色检测(双染)作为一种相对客观的检测方法,已在多个国家获批应用于临床。本共识基于循证医学证据,对双染样本的取材与制备、检测流程、结果判读及报告规范达成共识,并推荐双染可用于高危人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)初筛不分型阳性或部分分型其他12种(HR 12)阳性人群的分流;也可用于联合筛查时HR-HPV不分型阳性或部分分型HR 12阳性,且细胞学正常或轻度异常时的人群分流;还可用于筛查结果异常(HR-HPV检测或HR-HPV联合细胞学检测)后随访人群的分流。通过本共识的制定,旨在规范双染检测技术的临床应用,实施子宫颈癌精准筛查。
基金Supported by the National Research Foundation of Korea,No.2020R1A2C1100891Soonchunhyang University Research Fund,No.2024-05-014.
文摘BACKGROUND The high prevalence of human papillomavirus(HPV)infection in oropharyngeal squamous cell carcinoma(SCC)is well established,and p16 expression is a strong predictor.HPV-related tumors exhibit unique mechanisms that target p16 and p53 proteins.However,research on HPV prevalence and the combined predictive value of p16 and p53 expression in head and neck cutaneous SCC(HNCSCC),particularly in Asian populations,remains limited.This retrospective study surveyed 62 patients with HNSCC(2011-2020),excluding those with facial warts or other skin cancer.AIM To explore the prevalence of HPV and the predictive value of p16 and p53 expression in HNCSCC in Asian populations.METHODS All patients underwent wide excision and biopsy.Immunohistochemical staining for HPV,p16,and p53 yielded positive and negative results.The relevance of each marker was investigated by categorizing the tumor locations into high-risk and middle-risk zones based on recurrence frequency.RESULTS Of the 62 patients,20(32.26%)were male,with an average age of 82.27 years(range 26-103 years).High-risk included 19 cases(30.65%),with the eyelid and lip being the most common sites(five cases,8.06%).Middle-risk included 43 cases(69.35%),with the cheek being the most common(29 cases,46.77%).The p16 expression was detected in 24 patients(38.71%),p53 expression in 42 patients(72.58%),and HPV in five patients(8.06%).No significant association was found between p16 expression and the presence of HPV(P>0.99),with a positive predictive value of 8.33%.CONCLUSION This study revealed that p16,a surrogate HPV marker in oropharyngeal SCC,is not reliable in HNCSCC,providing valuable insights for further research in Asian populations.
