There is nonradiative recombination in waveguide region owing to severe carrier leakage,which in turn reduces output power and wall-plug efficiency.In this paper,we designed a novel epitaxial structure,which suppresse...There is nonradiative recombination in waveguide region owing to severe carrier leakage,which in turn reduces output power and wall-plug efficiency.In this paper,we designed a novel epitaxial structure,which suppresses carrier leakage by inserting n-Ga_(0.55)In_(0.45)P and p-GaAs_(0.6)P_(0.4) between barriers and waveguide layers,respectively,to modulate the energy band structure and to increase the height of barrier.The results show that the leakage current density reduces by 87.71%,compared to traditional structure.The nonradiative recombination current density of novel structure reduces to 37.411 A/cm^(2),and the output power reaches 12.80 W with wall-plug efficiency of 78.24%at an injection current density 5 A/cm^(2) at room temperature.In addition,the temperature drift coefficient of center wavelength is 0.206 nm/℃at the temperature range from 5℃to 65℃,and the slope of fitted straight line of threshold current with temperature variation is 0.00113.The novel epitaxial structure provides a theoretical basis for achieving high-power laser diode.展开更多
BACKGROUND Colorectal cancer(CRC)is a leading cause of cancer-related morbidity and mor-tality globally.Exosomal microRNAs(miRNAs)are known to modulate tumor progression by influencing immune responses and vascular dy...BACKGROUND Colorectal cancer(CRC)is a leading cause of cancer-related morbidity and mor-tality globally.Exosomal microRNAs(miRNAs)are known to modulate tumor progression by influencing immune responses and vascular dynamics.However,the roles of specific exosomal miRNAs,such as miR-425-5p and miR-135b-3p,in CRC remain unclear.AIM To explore the specific roles and underlying mechanisms of exosomal miR-425-5p and miR-135b-3p in CRC progression.METHODS Differentially expressed miRNAs were identified through microarray analysis of exosomes isolated from CRC tissues and adjacent normal mucosa.Functional roles of miR-425-5p and miR-135b-3p were evaluated in vitro using macrophage polarization,T cell differentiation,and vascular permeability assays,as well as in vivo tumor formation and metastasis experiments in nude mice.Validation expe-riments were performed using CRC cell lines(HCT116 and SW620).RESULTS Exosomal miR-425-5p and miR-135b-3p were significantly upregulated in CRC compared to normal tissues.Functional studies revealed that miR-425-5p promo-tes macrophage M2-like polarization and suppresses T cell proinflammatory responses,while miR-135b-3p enhances vascular permeability and angiogenesis.Inhibition of these miRNAs in CRC cell-derived exosomes significantly supp-ressed tumor growth and metastasis in nude mice,reprogramming the tumor microenvironment toward reduced angiogenesis and enhanced immune acti-vation.Combined inhibition of both miRNAs resulted in the most pronounced effects.CONCLUSION Exosomal miR-425-5p and miR-135b-3p drive CRC progression by promoting immune suppression and vascular permeability.Their inhibition offers a promising strategy for modulating the tumor microenvironment and limiting CRC metastasis.展开更多
Spermatogonial stem cells(SSCs)are the key to maintaining production of the sperms and healthy offsprings,and also treating breeding livestock's reproductive damage and infertility.micro RNAs act a decisive role i...Spermatogonial stem cells(SSCs)are the key to maintaining production of the sperms and healthy offsprings,and also treating breeding livestock's reproductive damage and infertility.micro RNAs act a decisive role in regulating gene expression in many cells and tissues,including in processes such as proliferation,self-renewal,differentiation,and apoptosis of stem cells.However,the miRNA mechanism in regulation of SSCs is still unclear.Here,high-throughput sequencing was used to identify specific miRNAs.We confirmed that miR-21-5p was concentrated in both goat and mouse SSCs,and enhanced the proliferation and antiapoptotic ability of SSCs.In vivo experiments have shown that miR-21-5p resisted the damage of the chemotherapy drug Busulfan to germ cells,ameliorated Busulfan-induced testicular dysfunction,and maintained spermatogenesis.Further RNA-seq and target gene prediction revealed that SPRY1 and FASLG are targets of miR-21-5p,thereby activating downstream signaling pathways such as MAPK/ERK,PI3K-AKT,and apoptosis.In summary,miR-21-5p is crucial for the self-renewal and maintenance of SSCs.This study provides new avenues for treating breeding livestock's reproductive damages,infertility,oligospermia,and other conditions.展开更多
Exosomes derived from hypoxic endometrial epithelial cells are pivotal in cellular communication and tissue repair,offering new perspectives on reproductive health.This manuscript highlights the study by Zhang et al,w...Exosomes derived from hypoxic endometrial epithelial cells are pivotal in cellular communication and tissue repair,offering new perspectives on reproductive health.This manuscript highlights the study by Zhang et al,which investigates the effects of miR-214-5p and miR-21-5p in hypoxic cell-derived exosomes on human umbilical cord mesenchymal stem cells.The study reveals that low levels of these microRNAs activate the signal transducer and activator of transcription 3 signaling pathway,enhancing human umbilical cord mesenchymal stem cell migration and differentiation.These findings provide novel insights into therapeutic strategies for improving endometrial health and addressing infertility linked to thin endometrium.展开更多
Colorectal cancer(CRC)ranks among the top five most common malignant tumors worldwide and has a high mortality rate.Angiogenesis plays an important role in CRC progression;however,anti-angiogenesis therapy still has m...Colorectal cancer(CRC)ranks among the top five most common malignant tumors worldwide and has a high mortality rate.Angiogenesis plays an important role in CRC progression;however,anti-angiogenesis therapy still has many limitations.Long non-coding RNAs(lncRNAs)participate in tumor progression by regulating the expression of vascular endothelial growth factor in metastatic CRC.Thus,targeting specific lncRNAs may provide some new hope for anti-angiogenic strategies.Through analyzing data from both clinical samples and The Cancer Genome Atlas database,we found that the lncRNA LINC01503 was specifically upregulated in CRC tissues and was associated with tumor progression and poor overall survival.We also demonstrated that LINC01503 enhanced the capacity for tube formation and migration of vascular endothelial cells,thus promoting CRC tumorigenesis by upregulating vascular endothelial growth factor A(VEGFA)expression in CRC cells.Mechanistically,LINC01503 promoted the expression of VEGFA by simultaneously regulating both mRNA and protein stability of VEGFA by binding to miR-342-3p and the chaperone HSP60,respectively.The upregulation of LINC01503 in CRC cells was attributed to the CREB-binding protein CBP/p300-mediated H3K27 acetylation of the LINC01503 promoter region.Taken together,our findings clarify the mechanism by which LINC01503 may promote CRC angiogenesis,implying that LINC01503 may serve as a potential prognostic biomarker and therapeutic target for CRC.展开更多
Objectives:Chemotherapy-induced lung inflammation limits the efficacy of anticancer therapies such as gefitinib in non-small cell lung cancer(NSCLC).Glutamic acid-leucine-arginine positive(ELR+)CXC chemokines and thei...Objectives:Chemotherapy-induced lung inflammation limits the efficacy of anticancer therapies such as gefitinib in non-small cell lung cancer(NSCLC).Glutamic acid-leucine-arginine positive(ELR+)CXC chemokines and their receptors,CXC chemokine receptor 1 and 2(CXCR1 and CXCR2),mediate both inflammatory responses and tumor progression.This study evaluated the effects of CXCR1/2 antagonism by G31P,a CXC motif chemokine ligand 8(CXCL8)-mutated peptide,alone or in combination with gefitinib,on lung cancer growth and chemotherapyinduced pulmonary inflammation.Methods:Human NSCLC cell lines(A549 and H460)were treated with gefitinib and/or G31P.Cell proliferation,apoptosis,and signaling pathways,including protein kinase B(AKT)and extracellular signal-regulated kinase(ERK)phosphorylation,were evaluated by cell counting kit-8(CCK-8)assay,flow cytometry,and Western blotting.An orthotopic lung tumor xenograft model was established in BALB/c nude mice to evaluate tumor growth,metastasis,cytokine expression,and lung histopathology.A bleomycin-induced lung injury model was also used to assess the anti-inflammatory effects of G31P,with or without gefitinib,by quantitative reverse transcription polymerase chain reaction(qRT-PCR)and flowcytometry of inflammatorymarkers.Results:G31P andGefitinib,either alone or combined,inhibited proliferation and migration of A549 and H460 cells in vitro.Combination treatment effectively reducedAKT and ERKphosphorylation in both cell lines.In vivo,G31Pwith gefitinib significantly suppressed tumor growth,metastasis,and increased apoptosis.G31P decreased CXCL1 and CXCL2,and tumor necrosis factoralpha(TNF-α)mRNA levels,lung hydroxyproline content,and myeloperoxidase(MPO)activity in the lungs of mice.In the bleomycin-induced lung injury model,G31P similarly reduced inflammatory responses.Conclusion:CXCR1/2 antagonismby G31P attenuates chemotherapy-induced pulmonary inflammation and enhances the anti-tumor efficacy of gefitinib in NSCLC.These findings support the therapeutic potential of G31P as an adjuvant to epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKIs)to improve clinical outcomes by limiting inflammation.展开更多
Hepatocellular carcinoma(HCC)shows the highest morbidity among liver cancers which is characterized by genetic mutations in hepatocytes,leading to uncontrolled cell growth and proliferation.Current treatment include s...Hepatocellular carcinoma(HCC)shows the highest morbidity among liver cancers which is characterized by genetic mutations in hepatocytes,leading to uncontrolled cell growth and proliferation.Current treatment include surgery,chemotherapy and immunotherapy;however,chemotherapeutics,which focus on single-targeted drug therapy,are still associated with certain limitations and may affect the treatment outcomes.Natural products also show the anticancer effect of HCC and hypotoxicity,but overall low activity of natural products limits their further application.miRNAs canmodulate post-transcriptional functions of target genes.An increasing body of evidence has demonstrated that miRNAs are the key regulators in HCC by targeting different molecules in different signaling pathways.However,miRNAs are fragile and liable to catabolism by RNases in serum and other body fluids,and small molecules separated from natural products may have limited bioavailability.A chitosan based,targeted,sustained-release nanoparticle deliverymiR-128–3p agomir(NA-miR-128–3p)was developed in this work.This nanoparticle was prepared by pentasodium tripolyphosphate(TPP),chitosan hydrochloride and miR-128–3p agomir with target aptamer which was loaded into the chitosan nanoparticle by self-assembly.It can intervene in HCC progress by affecting AKT1 expression.Based on this,a novel,efficient,long-acting,multi-mechanism and low-dosage combination drug delivery strategy was proposed in thiswork and showed a prominent anti-tumor effect.NA-miR-128–3p combined with natural product Oroxin B significantly affected HCC progression by the interference with VEGF and PI3K-AKT pathways,better than using NA-miR-128–3p and Oroxin B alone.Taken together,this nanoparticle and combinative administration compensate for the shortcomings of the fragile RNA drugs and the low activity of natural products,with high prospects in HCC treatment.展开更多
AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control...AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.展开更多
Silicosis,a major persistent occupational disease in China,is a progressive and irreversible pulmonary fibrosis disease with unclear pathogenesis.Cellular senescence,a state of stable cell cycle arrest that is recogni...Silicosis,a major persistent occupational disease in China,is a progressive and irreversible pulmonary fibrosis disease with unclear pathogenesis.Cellular senescence,a state of stable cell cycle arrest that is recognized as a key underlying factor in age-related fibroproliferative disorders,plays an important role in chronic lung diseases,particularly pulmonary fibrosis.We previously reported that SiO2-stimulated mice and alveolar type II epithelial cells develop cellular senescence,which is involved in silicosis formation in alveolar type II epithelial cells[1].Cellular senescence may play an important role in silicosis development;however,the exact underlying mechanisms are not fully understood.展开更多
[Objective] This study was conducted to investigate the demands for N, P and K by wheat in wheat cotton intercropping system in high-fertility field of Hebei Province. [Method] The experiment adopted randomized block ...[Objective] This study was conducted to investigate the demands for N, P and K by wheat in wheat cotton intercropping system in high-fertility field of Hebei Province. [Method] The experiment adopted randomized block arrangement. Five treatments （0, 75, 150, 225 and 300 kg/hm2） were designed for N, P and K. Wheat tillering, ear number, yield and yield components were investigated. [Result] Wheat yield increased significantly with the application of N fertilizer. The number of ears per unit area, number of grains per ear, 1 000-grain weight and yield were im- proved with N increasing. P fertilizer improved wheat yield at a certain degree, but K had no effect on wheat yield. ]Conclusion] The optimum fertilization for wheat-cot- ton intercropping system was N at 225-300 kg/hm2 and P2Os at 150 kg/hm2; and there is no need to apply K.展开更多
The effects of phosphorus and boron addition on the as-cast microstructure and homogenization parameters of Inconel 718 were studied. The results indicate that the addition of phosphorus and boron promotes the formati...The effects of phosphorus and boron addition on the as-cast microstructure and homogenization parameters of Inconel 718 were studied. The results indicate that the addition of phosphorus and boron promotes the formation of blocky Laves phase. Due to the strong segregation behavior of boron in the final residual liquid, a low melting B-bearing phase enriched in Nb, Mo and Cr is observed. According to the differential scanning calorimeter results and electron probe micro-analysis characterization, the solidification sequence of Inconel 718 with phosphorus and boron addition in best combination is determined as L→L＋γ→L＋γ＋MC→L＋γ＋MC＋Laves→γ＋MC＋Laves＋MC＋Laves＋B-bearing phase. Accordingly, the homogenization temperature is recommended to be adjusted at least 40°C lower than that of standard Inconel 718 due to the existence of low melting B-bearing phase.展开更多
Phosphorus （P） applied from fertilizer and manure is important in increasing crop yield and soil fertility; however, excessive uses of phosphate fertilizer and manure may also increase P loss from agricultural soils...Phosphorus （P） applied from fertilizer and manure is important in increasing crop yield and soil fertility; however, excessive uses of phosphate fertilizer and manure may also increase P loss from agricultural soils, posing environmental impact. A long term experiment was conducted on a calcareous soil （meadow cinnamon） in Hebei Province, China, from 2003 to 2006 to investigate the effects of phosphate fertilizer and manure on the yield of Chinese cabbage, soil P accumulation, P sorption saturation, soluble P in runoff water, and P leaching. P fertilizer （P2O5） application at a rate of 360 kg ha^-1 or manure of 150 t ha^-1 significantly increased Chinese cabbage yield as compared to the unfertilized control. However, no significant yield response was found with excessive phosphate or manure application. Soil Olsen-P, soluble P, bioavailable P, the degree of phosphorus sorption saturation in top soil layer （0-20 cm）, and soluble P in runoff water increased significantly with the increase of phosphate fertilizer and manure application rates, whereas the maximum phosphorus sorption capacity （Qm） decreased with the phosphate fertilizer and manure application rates. Soil Olsen-P and soluble P also increased significantly in the sub soil layer （20-40 cm） with the high P fertilizer and manure rates. It indicates that excessive P application over crop demand can lead to a high environmental risk owing to the enrichment of soil Olsen-P, soluble P, bioavailable P, and the degree of phosphorus sorption saturation in agricultural soils.展开更多
The effect of seawater on vertical ground motions is studied via a theoretical method and then actual offshore ground motion records are analyzed using a statistical method. A theoretical analysis of the effect of sea...The effect of seawater on vertical ground motions is studied via a theoretical method and then actual offshore ground motion records are analyzed using a statistical method. A theoretical analysis of the effect of seawater on incident plane P and SV waves at ocean bottom indicate that on one hand, the affected frequency range of vertical ground motions is prominent due to P wave resonance in the water layer if the impedance ratio between the seawater and the underlying medium is large, but it is greatly suppressed if the impedance ratio is small; on the other hand, for the ocean bottom interface model selected herein, vertical ground motions consisting of mostly P waves are more easily affected by seawater than those dominated by SV waves. The statistical analysis of engineering parameters of offshore ground motion records indicate that:(1) Under the infl uence of softer surface soil at the seafl oor, both horizontal and vertical spectral accelerations of offshore motions are exaggerated at long period components, which leads to the peak spectral values moving to a longer period.(2) The spectral ratios(V/H) of offshore ground motions are much smaller than onshore ground motions near the P wave resonant frequencies in the water layer; and as the period becomes larger, the effect of seawater becomes smaller, which leads to a similar V/H at intermediate periods(near 2 s). These results are consistent with the conclusions of Boore and Smith(1999), but the V/H of offshore motion may be smaller than the onshore ground motions at longer periods(more than 5 s).展开更多
Manuscript received 24 June 1999 The Fe Al P alloy deposits were plated on copper sheets by electroless plating. The change law of the deposition rate, composition, surface appearance and structure for the depo...Manuscript received 24 June 1999 The Fe Al P alloy deposits were plated on copper sheets by electroless plating. The change law of the deposition rate, composition, surface appearance and structure for the deposits was studied by changing the metallic salt ratios (AlCl 3/AlCl 3+FeSO 4), the concentration of metallic salt AlCl 3 and reductant NaH 2PO 2. The optimum plating bath was obtained. It was found that the choices of ligand and reductant were the key of increasing Al content for the deposits.展开更多
Vesicular-arbuscular mycorrhizal (VAM) fungi have been credited with improving the groWth and mineral nutrition of many host plants but these effects are moderated by soil factors and nutrient balance. The combined ef...Vesicular-arbuscular mycorrhizal (VAM) fungi have been credited with improving the groWth and mineral nutrition of many host plants but these effects are moderated by soil factors and nutrient balance. The combined effects of VAM, Zn and P application on the growth and translocation of nutrients in wheat were investigated using a calcareous soil marginal in P and Zn concentrations. Wheat was grown in a growth chamber under various combinations of VAM, P and Zn with measurements done at heading stage and maturity.Vegetative dry matter accumulation was increased by P application and reduced by VAM treatments. Both P and VAM increased grain yield. Zinc concentration and uptake were generally reduced by P addition and VAM infection. There were no antagonistic effects of Zn on P acquisition in the plant. The role of VAM in enhancing the translocation of Zn and P from root to grain would be beneficial to seed setting and yield.展开更多
The role of Cr in affecting the precipitates and the properties of aged Cu−Cr−P alloys was investigated and discussed.The results show that there are mainly three sizes of Cr phase in aged Cu−Cr−P alloys,among them,th...The role of Cr in affecting the precipitates and the properties of aged Cu−Cr−P alloys was investigated and discussed.The results show that there are mainly three sizes of Cr phase in aged Cu−Cr−P alloys,among them,the nano-sized Cr phase plays an important role in the strength of Cu−Cr−P alloys.The strengthening effect of Cr phase(less than 5 nm)with FCC structure completely coherent with the matrix is calculated to be about 200 MPa on the basis of dislocation cut-through mechanism.The strengthening effect of Cr phase(10−20 nm)with BCC structure incoherent with the matrix is calculated to be about 100 MPa on the basis of the Orowan dislocation bypass mechanism.The increase of Cr content changes the number and size of nano-sized Cr phase,which causes the mechanical properties of the Cu−Cr−P alloys to increase first and then decrease.The tensile strength of Cu−0.36Cr−0.01P alloy is 572 MPa and its electrical conductivity is 80%IACS after solid solution treatment at 980°C for 2 h followed by 95%cold rolling and then aging treatment at 450°C for 1 h.展开更多
文摘There is nonradiative recombination in waveguide region owing to severe carrier leakage,which in turn reduces output power and wall-plug efficiency.In this paper,we designed a novel epitaxial structure,which suppresses carrier leakage by inserting n-Ga_(0.55)In_(0.45)P and p-GaAs_(0.6)P_(0.4) between barriers and waveguide layers,respectively,to modulate the energy band structure and to increase the height of barrier.The results show that the leakage current density reduces by 87.71%,compared to traditional structure.The nonradiative recombination current density of novel structure reduces to 37.411 A/cm^(2),and the output power reaches 12.80 W with wall-plug efficiency of 78.24%at an injection current density 5 A/cm^(2) at room temperature.In addition,the temperature drift coefficient of center wavelength is 0.206 nm/℃at the temperature range from 5℃to 65℃,and the slope of fitted straight line of threshold current with temperature variation is 0.00113.The novel epitaxial structure provides a theoretical basis for achieving high-power laser diode.
文摘BACKGROUND Colorectal cancer(CRC)is a leading cause of cancer-related morbidity and mor-tality globally.Exosomal microRNAs(miRNAs)are known to modulate tumor progression by influencing immune responses and vascular dynamics.However,the roles of specific exosomal miRNAs,such as miR-425-5p and miR-135b-3p,in CRC remain unclear.AIM To explore the specific roles and underlying mechanisms of exosomal miR-425-5p and miR-135b-3p in CRC progression.METHODS Differentially expressed miRNAs were identified through microarray analysis of exosomes isolated from CRC tissues and adjacent normal mucosa.Functional roles of miR-425-5p and miR-135b-3p were evaluated in vitro using macrophage polarization,T cell differentiation,and vascular permeability assays,as well as in vivo tumor formation and metastasis experiments in nude mice.Validation expe-riments were performed using CRC cell lines(HCT116 and SW620).RESULTS Exosomal miR-425-5p and miR-135b-3p were significantly upregulated in CRC compared to normal tissues.Functional studies revealed that miR-425-5p promo-tes macrophage M2-like polarization and suppresses T cell proinflammatory responses,while miR-135b-3p enhances vascular permeability and angiogenesis.Inhibition of these miRNAs in CRC cell-derived exosomes significantly supp-ressed tumor growth and metastasis in nude mice,reprogramming the tumor microenvironment toward reduced angiogenesis and enhanced immune acti-vation.Combined inhibition of both miRNAs resulted in the most pronounced effects.CONCLUSION Exosomal miR-425-5p and miR-135b-3p drive CRC progression by promoting immune suppression and vascular permeability.Their inhibition offers a promising strategy for modulating the tumor microenvironment and limiting CRC metastasis.
基金supported by the National Natural Science Foundation of China(32072806 and 32372970)the National Key Research and Development Program of China(2022YFD1302201 and 2023YFF1000904)+3 种基金the Program of Shaanxi Province Science and Technology Innovation Team,China(2019TD-036)the Major Projects of Natural Science Foundation of Inner Mongolia Autonomous Region,China(2020ZD10)the Inner Mongolia Autonomous Region Competition Leaders,China(2022JBGS0025)Key Technologies Demonstration of Animal Husbandry in Shaanxi Province,China(20221086 and 20230978)。
文摘Spermatogonial stem cells(SSCs)are the key to maintaining production of the sperms and healthy offsprings,and also treating breeding livestock's reproductive damage and infertility.micro RNAs act a decisive role in regulating gene expression in many cells and tissues,including in processes such as proliferation,self-renewal,differentiation,and apoptosis of stem cells.However,the miRNA mechanism in regulation of SSCs is still unclear.Here,high-throughput sequencing was used to identify specific miRNAs.We confirmed that miR-21-5p was concentrated in both goat and mouse SSCs,and enhanced the proliferation and antiapoptotic ability of SSCs.In vivo experiments have shown that miR-21-5p resisted the damage of the chemotherapy drug Busulfan to germ cells,ameliorated Busulfan-induced testicular dysfunction,and maintained spermatogenesis.Further RNA-seq and target gene prediction revealed that SPRY1 and FASLG are targets of miR-21-5p,thereby activating downstream signaling pathways such as MAPK/ERK,PI3K-AKT,and apoptosis.In summary,miR-21-5p is crucial for the self-renewal and maintenance of SSCs.This study provides new avenues for treating breeding livestock's reproductive damages,infertility,oligospermia,and other conditions.
基金the National Natural Science Foundation of China,No.82170406 and No.81970238the Royal Society United Kingdom,No.IEC\NSFC\201094.
文摘Exosomes derived from hypoxic endometrial epithelial cells are pivotal in cellular communication and tissue repair,offering new perspectives on reproductive health.This manuscript highlights the study by Zhang et al,which investigates the effects of miR-214-5p and miR-21-5p in hypoxic cell-derived exosomes on human umbilical cord mesenchymal stem cells.The study reveals that low levels of these microRNAs activate the signal transducer and activator of transcription 3 signaling pathway,enhancing human umbilical cord mesenchymal stem cell migration and differentiation.These findings provide novel insights into therapeutic strategies for improving endometrial health and addressing infertility linked to thin endometrium.
基金supported by the National Natural Science Foundation of China(Grant No.81972288)the Scientific and Technologic Development Programme of Suzhou(Livelihood Science and Technology-Applied Basic Research in Healthcare,SYS2020057).
文摘Colorectal cancer(CRC)ranks among the top five most common malignant tumors worldwide and has a high mortality rate.Angiogenesis plays an important role in CRC progression;however,anti-angiogenesis therapy still has many limitations.Long non-coding RNAs(lncRNAs)participate in tumor progression by regulating the expression of vascular endothelial growth factor in metastatic CRC.Thus,targeting specific lncRNAs may provide some new hope for anti-angiogenic strategies.Through analyzing data from both clinical samples and The Cancer Genome Atlas database,we found that the lncRNA LINC01503 was specifically upregulated in CRC tissues and was associated with tumor progression and poor overall survival.We also demonstrated that LINC01503 enhanced the capacity for tube formation and migration of vascular endothelial cells,thus promoting CRC tumorigenesis by upregulating vascular endothelial growth factor A(VEGFA)expression in CRC cells.Mechanistically,LINC01503 promoted the expression of VEGFA by simultaneously regulating both mRNA and protein stability of VEGFA by binding to miR-342-3p and the chaperone HSP60,respectively.The upregulation of LINC01503 in CRC cells was attributed to the CREB-binding protein CBP/p300-mediated H3K27 acetylation of the LINC01503 promoter region.Taken together,our findings clarify the mechanism by which LINC01503 may promote CRC angiogenesis,implying that LINC01503 may serve as a potential prognostic biomarker and therapeutic target for CRC.
基金supported by grants from the National Natural Science Foundation of China,No.U1604174Health Commission of Henan Province-Province and Ministry Co-Construction Project,Nos.20170123,SBGJ202002004Health Commission of Henan Province-Research Innovative Talents Project(51282).
文摘Objectives:Chemotherapy-induced lung inflammation limits the efficacy of anticancer therapies such as gefitinib in non-small cell lung cancer(NSCLC).Glutamic acid-leucine-arginine positive(ELR+)CXC chemokines and their receptors,CXC chemokine receptor 1 and 2(CXCR1 and CXCR2),mediate both inflammatory responses and tumor progression.This study evaluated the effects of CXCR1/2 antagonism by G31P,a CXC motif chemokine ligand 8(CXCL8)-mutated peptide,alone or in combination with gefitinib,on lung cancer growth and chemotherapyinduced pulmonary inflammation.Methods:Human NSCLC cell lines(A549 and H460)were treated with gefitinib and/or G31P.Cell proliferation,apoptosis,and signaling pathways,including protein kinase B(AKT)and extracellular signal-regulated kinase(ERK)phosphorylation,were evaluated by cell counting kit-8(CCK-8)assay,flow cytometry,and Western blotting.An orthotopic lung tumor xenograft model was established in BALB/c nude mice to evaluate tumor growth,metastasis,cytokine expression,and lung histopathology.A bleomycin-induced lung injury model was also used to assess the anti-inflammatory effects of G31P,with or without gefitinib,by quantitative reverse transcription polymerase chain reaction(qRT-PCR)and flowcytometry of inflammatorymarkers.Results:G31P andGefitinib,either alone or combined,inhibited proliferation and migration of A549 and H460 cells in vitro.Combination treatment effectively reducedAKT and ERKphosphorylation in both cell lines.In vivo,G31Pwith gefitinib significantly suppressed tumor growth,metastasis,and increased apoptosis.G31P decreased CXCL1 and CXCL2,and tumor necrosis factoralpha(TNF-α)mRNA levels,lung hydroxyproline content,and myeloperoxidase(MPO)activity in the lungs of mice.In the bleomycin-induced lung injury model,G31P similarly reduced inflammatory responses.Conclusion:CXCR1/2 antagonismby G31P attenuates chemotherapy-induced pulmonary inflammation and enhances the anti-tumor efficacy of gefitinib in NSCLC.These findings support the therapeutic potential of G31P as an adjuvant to epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKIs)to improve clinical outcomes by limiting inflammation.
基金supported by the National Natural Science Foundation of China(Grant 82373828)the National Key Technologies R&D Program of China(2023YFD1400902).
文摘Hepatocellular carcinoma(HCC)shows the highest morbidity among liver cancers which is characterized by genetic mutations in hepatocytes,leading to uncontrolled cell growth and proliferation.Current treatment include surgery,chemotherapy and immunotherapy;however,chemotherapeutics,which focus on single-targeted drug therapy,are still associated with certain limitations and may affect the treatment outcomes.Natural products also show the anticancer effect of HCC and hypotoxicity,but overall low activity of natural products limits their further application.miRNAs canmodulate post-transcriptional functions of target genes.An increasing body of evidence has demonstrated that miRNAs are the key regulators in HCC by targeting different molecules in different signaling pathways.However,miRNAs are fragile and liable to catabolism by RNases in serum and other body fluids,and small molecules separated from natural products may have limited bioavailability.A chitosan based,targeted,sustained-release nanoparticle deliverymiR-128–3p agomir(NA-miR-128–3p)was developed in this work.This nanoparticle was prepared by pentasodium tripolyphosphate(TPP),chitosan hydrochloride and miR-128–3p agomir with target aptamer which was loaded into the chitosan nanoparticle by self-assembly.It can intervene in HCC progress by affecting AKT1 expression.Based on this,a novel,efficient,long-acting,multi-mechanism and low-dosage combination drug delivery strategy was proposed in thiswork and showed a prominent anti-tumor effect.NA-miR-128–3p combined with natural product Oroxin B significantly affected HCC progression by the interference with VEGF and PI3K-AKT pathways,better than using NA-miR-128–3p and Oroxin B alone.Taken together,this nanoparticle and combinative administration compensate for the shortcomings of the fragile RNA drugs and the low activity of natural products,with high prospects in HCC treatment.
基金Supported by the National Natural Science Foundation of China(No.81770898).
文摘AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.
文摘Silicosis,a major persistent occupational disease in China,is a progressive and irreversible pulmonary fibrosis disease with unclear pathogenesis.Cellular senescence,a state of stable cell cycle arrest that is recognized as a key underlying factor in age-related fibroproliferative disorders,plays an important role in chronic lung diseases,particularly pulmonary fibrosis.We previously reported that SiO2-stimulated mice and alveolar type II epithelial cells develop cellular senescence,which is involved in silicosis formation in alveolar type II epithelial cells[1].Cellular senescence may play an important role in silicosis development;however,the exact underlying mechanisms are not fully understood.
基金Supported by Science and Technology Demonstration Project of Bohai Granary from Ministry of Science and Technology(2013BAD05B00)Special Fund for Cotton Industry Technological System Construction(CARS-18-21)
文摘[Objective] This study was conducted to investigate the demands for N, P and K by wheat in wheat cotton intercropping system in high-fertility field of Hebei Province. [Method] The experiment adopted randomized block arrangement. Five treatments （0, 75, 150, 225 and 300 kg/hm2） were designed for N, P and K. Wheat tillering, ear number, yield and yield components were investigated. [Result] Wheat yield increased significantly with the application of N fertilizer. The number of ears per unit area, number of grains per ear, 1 000-grain weight and yield were im- proved with N increasing. P fertilizer improved wheat yield at a certain degree, but K had no effect on wheat yield. ]Conclusion] The optimum fertilization for wheat-cot- ton intercropping system was N at 225-300 kg/hm2 and P2Os at 150 kg/hm2; and there is no need to apply K.
基金Project (08dj1400402) supported by the Major Program for the Fundamental Research of Shanghai Committee of Science and Technology,China
文摘The effects of phosphorus and boron addition on the as-cast microstructure and homogenization parameters of Inconel 718 were studied. The results indicate that the addition of phosphorus and boron promotes the formation of blocky Laves phase. Due to the strong segregation behavior of boron in the final residual liquid, a low melting B-bearing phase enriched in Nb, Mo and Cr is observed. According to the differential scanning calorimeter results and electron probe micro-analysis characterization, the solidification sequence of Inconel 718 with phosphorus and boron addition in best combination is determined as L→L＋γ→L＋γ＋MC→L＋γ＋MC＋Laves→γ＋MC＋Laves＋MC＋Laves＋B-bearing phase. Accordingly, the homogenization temperature is recommended to be adjusted at least 40°C lower than that of standard Inconel 718 due to the existence of low melting B-bearing phase.
基金The study was supported by the 948 Program of theMinistry of Agriculture of China （2003-253） the Natural Science Foundation of Hebei Province,China （300130）.
文摘Phosphorus （P） applied from fertilizer and manure is important in increasing crop yield and soil fertility; however, excessive uses of phosphate fertilizer and manure may also increase P loss from agricultural soils, posing environmental impact. A long term experiment was conducted on a calcareous soil （meadow cinnamon） in Hebei Province, China, from 2003 to 2006 to investigate the effects of phosphate fertilizer and manure on the yield of Chinese cabbage, soil P accumulation, P sorption saturation, soluble P in runoff water, and P leaching. P fertilizer （P2O5） application at a rate of 360 kg ha^-1 or manure of 150 t ha^-1 significantly increased Chinese cabbage yield as compared to the unfertilized control. However, no significant yield response was found with excessive phosphate or manure application. Soil Olsen-P, soluble P, bioavailable P, the degree of phosphorus sorption saturation in top soil layer （0-20 cm）, and soluble P in runoff water increased significantly with the increase of phosphate fertilizer and manure application rates, whereas the maximum phosphorus sorption capacity （Qm） decreased with the phosphate fertilizer and manure application rates. Soil Olsen-P and soluble P also increased significantly in the sub soil layer （20-40 cm） with the high P fertilizer and manure rates. It indicates that excessive P application over crop demand can lead to a high environmental risk owing to the enrichment of soil Olsen-P, soluble P, bioavailable P, and the degree of phosphorus sorption saturation in agricultural soils.
基金the 973 Program under Grant No.2011CB013601Basic Science Research Foundation of IEM,CEA under Grant No.2011B02+1 种基金National Natural Science Foundation of China under Grant No.51238012,9121530113,50938006International Science&Technology Cooperation Program of China under Grant No.2012DFA70810
文摘The effect of seawater on vertical ground motions is studied via a theoretical method and then actual offshore ground motion records are analyzed using a statistical method. A theoretical analysis of the effect of seawater on incident plane P and SV waves at ocean bottom indicate that on one hand, the affected frequency range of vertical ground motions is prominent due to P wave resonance in the water layer if the impedance ratio between the seawater and the underlying medium is large, but it is greatly suppressed if the impedance ratio is small; on the other hand, for the ocean bottom interface model selected herein, vertical ground motions consisting of mostly P waves are more easily affected by seawater than those dominated by SV waves. The statistical analysis of engineering parameters of offshore ground motion records indicate that:(1) Under the infl uence of softer surface soil at the seafl oor, both horizontal and vertical spectral accelerations of offshore motions are exaggerated at long period components, which leads to the peak spectral values moving to a longer period.(2) The spectral ratios(V/H) of offshore ground motions are much smaller than onshore ground motions near the P wave resonant frequencies in the water layer; and as the period becomes larger, the effect of seawater becomes smaller, which leads to a similar V/H at intermediate periods(near 2 s). These results are consistent with the conclusions of Boore and Smith(1999), but the V/H of offshore motion may be smaller than the onshore ground motions at longer periods(more than 5 s).
文摘Manuscript received 24 June 1999 The Fe Al P alloy deposits were plated on copper sheets by electroless plating. The change law of the deposition rate, composition, surface appearance and structure for the deposits was studied by changing the metallic salt ratios (AlCl 3/AlCl 3+FeSO 4), the concentration of metallic salt AlCl 3 and reductant NaH 2PO 2. The optimum plating bath was obtained. It was found that the choices of ligand and reductant were the key of increasing Al content for the deposits.
文摘Vesicular-arbuscular mycorrhizal (VAM) fungi have been credited with improving the groWth and mineral nutrition of many host plants but these effects are moderated by soil factors and nutrient balance. The combined effects of VAM, Zn and P application on the growth and translocation of nutrients in wheat were investigated using a calcareous soil marginal in P and Zn concentrations. Wheat was grown in a growth chamber under various combinations of VAM, P and Zn with measurements done at heading stage and maturity.Vegetative dry matter accumulation was increased by P application and reduced by VAM treatments. Both P and VAM increased grain yield. Zinc concentration and uptake were generally reduced by P addition and VAM infection. There were no antagonistic effects of Zn on P acquisition in the plant. The role of VAM in enhancing the translocation of Zn and P from root to grain would be beneficial to seed setting and yield.
基金The authors are grateful for the financial supports from National Key R&D Program of China(2016YFB0301303)Beijing Nova Program,China(Z191100001119125)the National Natural Science Foundation of China(51974028,U1602271).
文摘The role of Cr in affecting the precipitates and the properties of aged Cu−Cr−P alloys was investigated and discussed.The results show that there are mainly three sizes of Cr phase in aged Cu−Cr−P alloys,among them,the nano-sized Cr phase plays an important role in the strength of Cu−Cr−P alloys.The strengthening effect of Cr phase(less than 5 nm)with FCC structure completely coherent with the matrix is calculated to be about 200 MPa on the basis of dislocation cut-through mechanism.The strengthening effect of Cr phase(10−20 nm)with BCC structure incoherent with the matrix is calculated to be about 100 MPa on the basis of the Orowan dislocation bypass mechanism.The increase of Cr content changes the number and size of nano-sized Cr phase,which causes the mechanical properties of the Cu−Cr−P alloys to increase first and then decrease.The tensile strength of Cu−0.36Cr−0.01P alloy is 572 MPa and its electrical conductivity is 80%IACS after solid solution treatment at 980°C for 2 h followed by 95%cold rolling and then aging treatment at 450°C for 1 h.
