BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl...BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl-2 and Bax expression in mice with OxyHb-induced subarachnoid hemorrhage. DESIGN, TIME AND SETTING： A completely randomized grouping, controlled animal experiment was performed at the Experimental Center for Biomedicine, College of Medicine, Xi＇an Jiaotong University between February and April 2005. MATERIALS： Fifty-four healthy, male, adult, ICR mice were included in this study. Subarachnoid hemorrhage was induced by a subarachnoid injection of OxyHb in 48 mice. Mouse NGF was obtained from Xiamen Beidazhilu Bioengineering Co., Ltd., China.METHODS： All 54 mice were randomly divided into three groups： control （n = 6）, injury （n = 24）, and NGF （n = 24）. The NGF group received a subarachnoidal administration of OxyHb, immediately followed by a caudal vein injection of NGF （1 μg）. The injury group was injected with OxyHb, and subsequently with physiological saline. The control group only received intravenous physiological saline. MAIN OUTCOME MEASURES： At 1,6, 24, and 48 hours following subarachnoid hemorrhage induction, expression levels of Bcl-2 and Bax were detected by immunohistochemistry in the cerebral cortex 3 mm anterior and posterior to the injection site. RESULTS： At all time points following OxyHb injection, cerebral cortical Bax levels were significantly higher in the injured group than in the control and NGF groups （P 〈 0.01）. During the first 24 hours following OxyHb injection, cerebral cortical Bcl-2 levels were significantly lower in the injury group compared to the control group （P 〈 0.05 0.01）. Between 1 and 48 hours, Bcl-2 levels were significantly higher in the NGF group than in the injury group （P 〈 0.01 ）. CONCLUSION： Exogenous NGF can inhibit increased neuronal Bax expression and decreased Bcl-2 expression in the cerebral cortex of mice with OxyHb -induced subarachnoid hemorrhage.展开更多
This work presents the use of extended Modified Lambert Beer(MLB)model for accurate andcontinuous monitoring of percent blood carboxyhemoglobin(COHb)(SCO)and oxyhemoglobin(OxyHb)saturation(SO,)via a fitting procedure....This work presents the use of extended Modified Lambert Beer(MLB)model for accurate andcontinuous monitoring of percent blood carboxyhemoglobin(COHb)(SCO)and oxyhemoglobin(OxyHb)saturation(SO,)via a fitting procedure.This quantification technique is based on theabsorption characteristics of hemoglobin derivatives in the wavelength range of 520-600 nm togive the best estimates of the required parameters.A comparison of the performance of the developed model and MILB law is made using attenuation data from Monte Carlo simulations for a two-layered skin model.The results revealed a lower mean absolute error of 0.4%in the valuesestimated by the developed model as compared to 10%that is given by the MILB law.This studyshowed that the discussed approach is able to provide consistent and accurate measurement ofblood SO,and SCO across diferent skin pigmen tations suggesting that it may potentially be usedas an alternative means for clinical diagnosis of carbon monoxide(CO)poisoning.展开更多
目的系统评价功能性近红外光谱技术(fNIRS)监测注意缺陷多动障碍(ADHD)患者的神经药理作用。方法计算机检索SinoMed、中国知网、Scopus、PubMed、Embase和Web of Science核心合集数据库中关于fNIRS监测ADHD患者氧合血红蛋白的随机对照试...目的系统评价功能性近红外光谱技术(fNIRS)监测注意缺陷多动障碍(ADHD)患者的神经药理作用。方法计算机检索SinoMed、中国知网、Scopus、PubMed、Embase和Web of Science核心合集数据库中关于fNIRS监测ADHD患者氧合血红蛋白的随机对照试验,检索时间为建库至2025年2月。比较不同处理措施对氧合血红蛋白的影响。利用Review Manager 5.4.1偏倚风险评估工具对纳入按照随机序列生成、分配隐藏等7个维度进行文献质量评价。结果共纳入5篇文献,涉及85人次药物干预患者、85人次安慰剂干预患者和52名健康群体。meta分析显示,未用药组与健康组氧合血红蛋白比较,差异有统计学意义(P<0.05);用药组和安慰剂组氧合血红蛋白比较,差异有统计学意义(P<0.05);治疗后1年,用药组和健康组氧合血红蛋白比较,差异无统计学意义(P>0.05)。结论fNIRS监测提示ADHD患者用药后可提升氧合血红蛋白水平至正常。展开更多
文摘BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl-2 and Bax expression in mice with OxyHb-induced subarachnoid hemorrhage. DESIGN, TIME AND SETTING： A completely randomized grouping, controlled animal experiment was performed at the Experimental Center for Biomedicine, College of Medicine, Xi＇an Jiaotong University between February and April 2005. MATERIALS： Fifty-four healthy, male, adult, ICR mice were included in this study. Subarachnoid hemorrhage was induced by a subarachnoid injection of OxyHb in 48 mice. Mouse NGF was obtained from Xiamen Beidazhilu Bioengineering Co., Ltd., China.METHODS： All 54 mice were randomly divided into three groups： control （n = 6）, injury （n = 24）, and NGF （n = 24）. The NGF group received a subarachnoidal administration of OxyHb, immediately followed by a caudal vein injection of NGF （1 μg）. The injury group was injected with OxyHb, and subsequently with physiological saline. The control group only received intravenous physiological saline. MAIN OUTCOME MEASURES： At 1,6, 24, and 48 hours following subarachnoid hemorrhage induction, expression levels of Bcl-2 and Bax were detected by immunohistochemistry in the cerebral cortex 3 mm anterior and posterior to the injection site. RESULTS： At all time points following OxyHb injection, cerebral cortical Bax levels were significantly higher in the injured group than in the control and NGF groups （P 〈 0.01）. During the first 24 hours following OxyHb injection, cerebral cortical Bcl-2 levels were significantly lower in the injury group compared to the control group （P 〈 0.05 0.01）. Between 1 and 48 hours, Bcl-2 levels were significantly higher in the NGF group than in the injury group （P 〈 0.01 ）. CONCLUSION： Exogenous NGF can inhibit increased neuronal Bax expression and decreased Bcl-2 expression in the cerebral cortex of mice with OxyHb -induced subarachnoid hemorrhage.
文摘This work presents the use of extended Modified Lambert Beer(MLB)model for accurate andcontinuous monitoring of percent blood carboxyhemoglobin(COHb)(SCO)and oxyhemoglobin(OxyHb)saturation(SO,)via a fitting procedure.This quantification technique is based on theabsorption characteristics of hemoglobin derivatives in the wavelength range of 520-600 nm togive the best estimates of the required parameters.A comparison of the performance of the developed model and MILB law is made using attenuation data from Monte Carlo simulations for a two-layered skin model.The results revealed a lower mean absolute error of 0.4%in the valuesestimated by the developed model as compared to 10%that is given by the MILB law.This studyshowed that the discussed approach is able to provide consistent and accurate measurement ofblood SO,and SCO across diferent skin pigmen tations suggesting that it may potentially be usedas an alternative means for clinical diagnosis of carbon monoxide(CO)poisoning.
文摘目的系统评价功能性近红外光谱技术(fNIRS)监测注意缺陷多动障碍(ADHD)患者的神经药理作用。方法计算机检索SinoMed、中国知网、Scopus、PubMed、Embase和Web of Science核心合集数据库中关于fNIRS监测ADHD患者氧合血红蛋白的随机对照试验,检索时间为建库至2025年2月。比较不同处理措施对氧合血红蛋白的影响。利用Review Manager 5.4.1偏倚风险评估工具对纳入按照随机序列生成、分配隐藏等7个维度进行文献质量评价。结果共纳入5篇文献,涉及85人次药物干预患者、85人次安慰剂干预患者和52名健康群体。meta分析显示,未用药组与健康组氧合血红蛋白比较,差异有统计学意义(P<0.05);用药组和安慰剂组氧合血红蛋白比较,差异有统计学意义(P<0.05);治疗后1年,用药组和健康组氧合血红蛋白比较,差异无统计学意义(P>0.05)。结论fNIRS监测提示ADHD患者用药后可提升氧合血红蛋白水平至正常。
基金supported by the National Natural Science Foundation of China (No. 10572159)the "111" Project (No. B06023)+1 种基金the National Science Program of Science and Technology Committee of Chongqing China (No. 2006ba5010)
