随着网络视频平台(network video platform,NVP)应用,网络视频在不同视频平台分享时常面临被侵权和跨平台版权检测问题,所以本文提出了一种基于区块链跨平台网络视频版权保护方案(blockchain-based crossplatform network video copyrig...随着网络视频平台(network video platform,NVP)应用,网络视频在不同视频平台分享时常面临被侵权和跨平台版权检测问题,所以本文提出了一种基于区块链跨平台网络视频版权保护方案(blockchain-based crossplatform network video copyright protection scheme,BCVCP)旨在通过区块链和利用生成的所有权序列(ownership sequence,OS),进行所有权序列检测,来实现跨视频平台的网络视频版权保护.本方案包括身份认证、提取关键帧、所有权序列的生成和检测、网络视频控制管理等部分.具体来说,在对网络视频上传或访问等操作之前,需要进行身份认证,确保身份信息的安全.其次,上传网络视频过程中会生成所有权序列,存储在分布式节点中.然后,提取视频关键帧,把生成的所有权序列嵌入到视频关键帧中.最后,调用智能合约进行跨平台所有权序列检测和对网络视频的传播控制管理,避免侵权行为.在实验中,验证了跨视频平台传输网络视频时所有权编码质量和所有权识别的鲁棒性,保护了网络视频的版权.展开更多
Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies hav...Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV.展开更多
文摘随着网络视频平台(network video platform,NVP)应用,网络视频在不同视频平台分享时常面临被侵权和跨平台版权检测问题,所以本文提出了一种基于区块链跨平台网络视频版权保护方案(blockchain-based crossplatform network video copyright protection scheme,BCVCP)旨在通过区块链和利用生成的所有权序列(ownership sequence,OS),进行所有权序列检测,来实现跨视频平台的网络视频版权保护.本方案包括身份认证、提取关键帧、所有权序列的生成和检测、网络视频控制管理等部分.具体来说,在对网络视频上传或访问等操作之前,需要进行身份认证,确保身份信息的安全.其次,上传网络视频过程中会生成所有权序列,存储在分布式节点中.然后,提取视频关键帧,把生成的所有权序列嵌入到视频关键帧中.最后,调用智能合约进行跨平台所有权序列检测和对网络视频的传播控制管理,避免侵权行为.在实验中,验证了跨视频平台传输网络视频时所有权编码质量和所有权识别的鲁棒性,保护了网络视频的版权.
基金the Open Research Fund Program of the State Key Laboratory of Virology of China(Grant No.2019IOV004)the key Research Program of Frontier Sciences of Chinese Academy of Sciences(Grant No.QYZDJ-SSW-SMC021)the National Natural Science Foundation of China(Grant No.31900154 and 31572471)。
文摘Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV.
