Downregulation of the inwardly rectifying potassium channel Kir4.1 is a key step for inducing retinal Müller cell activation and interaction with other glial cells,which is involved in retinal ganglion cell apopt...Downregulation of the inwardly rectifying potassium channel Kir4.1 is a key step for inducing retinal Müller cell activation and interaction with other glial cells,which is involved in retinal ganglion cell apoptosis in glaucoma.Modulation of Kir4.1 expression in Müller cells may therefore be a potential strategy for attenuating retinal ganglion cell damage in glaucoma.In this study,we identified seven predicted phosphorylation sites in Kir4.1 and constructed lentiviral expression systems expressing Kir4.1 mutated at each site to prevent phosphorylation.Following this,we treated Müller glial cells in vitro and in vivo with the m Glu R I agonist DHPG to induce Kir4.1 or Kir4.1 Tyr^(9)Asp overexpression.We found that both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited activation of Müller glial cells.Subsequently,we established a rat model of chronic ocular hypertension by injecting microbeads into the anterior chamber and overexpressed Kir4.1 or Kir4.1 Tyr^(9)Asp in the eye,and observed similar results in Müller cells in vivo as those seen in vitro.Both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited Müller cell activation,regulated the balance of Bax/Bcl-2,and reduced the m RNA and protein levels of pro-inflammatory factors,including interleukin-1βand tumor necrosis factor-α.Furthermore,we investigated the regulatory effects of Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression on the release of pro-inflammatory factors in a co-culture system of Müller glial cells and microglia.In this co-culture system,we observed elevated adenosine triphosphate concentrations in activated Müller cells,increased levels of translocator protein(a marker of microglial activation),and elevated interleukin-1βm RNA and protein levels in microglia induced by activated Müller cells.These changes could be reversed by Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression in Müller cells.Kir4.1 overexpression,but not Kir4.1 Tyr^(9)Asp overexpression,reduced the number of proliferative and migratory microglia induced by activated Müller cells.Collectively,these results suggest that the tyrosine residue at position nine in Kir4.1 may serve as a functional modulation site in the retina in an experimental model of glaucoma.Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression attenuated Müller cell activation,reduced ATP/P2X receptor–mediated interactions between glial cells,inhibited microglial activation,and decreased the synthesis and release of pro-inflammatory factors,consequently ameliorating retinal ganglion cell apoptosis in glaucoma.展开更多
The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Re...The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.595–603.展开更多
The published article titled“Overexpression of miR-1283 Inhibits Cell Proliferation and Invasion of Glioma Cells by Targeting ATF4”has been retracted from Oncology Research,Vol.27,No.3,2019,pp.325–334.
Correction to:Neurosci.Bull.December,2016,32(6):577–584.https://doi.org/10.1007/s12264-016-0068-z In this article,in Fig 5A,the picture of the Vector+Nogo-66 group was incorrect and should have appeared as shown below.
Drought and salt stresses are major abiotic factors that severely affect the growth,development,and yield formation of Brassica napus.Human derived FTO gene(Fat mass and obesity-associated),is a member of the Alk B fa...Drought and salt stresses are major abiotic factors that severely affect the growth,development,and yield formation of Brassica napus.Human derived FTO gene(Fat mass and obesity-associated),is a member of the Alk B family,encoding a m RNA demethylase that is dependent onα-ketoglutarate.Although previous studies have demonstrated that exogenous overexpression of FTO gene can increase plant biomass,its impact on plant stress resistance is still unclear.In this study,we cloned the FTO gene and conducted an analysis of its biological functions for drought and salt resistance for Brassicaceae plants.By overexpressing the FTO gene in Arabidopsis thaliana,the inhibitory effect of salt and drought stress on the root length growth of transgenic lines was significantly lower than that of the control.Moreover,the overexpression of FTO markedly enhanced the tolerance of Arabidopsis to drought and salt stress.It also led to a decrease in malondialdehyde(MDA)content,an increase in proline content,and a boost in superoxide dismutase(SOD)activity.Meanwhile,when the FTO gene was heterologously expressed in B.napus,the transgenic plants were less affected by stress.In comparison to control plants,they exhibited significantly lower MDA levels and markedly higher proline content and SOD activity.Furthermore,staining results with Trypan blue and nitroblue tetrazolium(NBT)staining indicate that the FTO gene can alleviate the damage to plants under stress and inhibit the accumulation of O_(2)^(-).Comprehensively,the results indicate that overexpression of the FTO gene can improve the drought and salt tolerance in transgenic plants,providing valuable references for further exploring the FTO-mediated stress resistance mechanisms.展开更多
A polyketide synthase-nonribosomal peptide synthetase gene cluster twn in Talaromyces sp.HDN1820200 was activated by overexpression of the pathway-specific transcriptional factor TwnD.Large-scale fermentation and chem...A polyketide synthase-nonribosomal peptide synthetase gene cluster twn in Talaromyces sp.HDN1820200 was activated by overexpression of the pathway-specific transcriptional factor TwnD.Large-scale fermentation and chemical investigation of the mutant strain HDN1820200/TwnD led to the discovery of one new polyketide-amino acid conjugate,bipolamide C and one new polyketide compound,variotin A.The structures of the new compounds were determined by nuclear magnetic resonance(NMR)analysis,high-resolution electrospray ionization mass spectrometry,feeding experiments,NMR calculation and DP4^(+)analysis.This study revealed that the overexpression of the pathway-specific transcriptional factor represents a promising approach for the discovery of new natural products in fungi within specialized habitat.展开更多
Malolactic fermentation,started by lactic acid bacteria,plays a crucial role in the production of high-quality wines.As global warming increases the ethanol content in wines,the success of malolactic fermentation depe...Malolactic fermentation,started by lactic acid bacteria,plays a crucial role in the production of high-quality wines.As global warming increases the ethanol content in wines,the success of malolactic fermentation depends on selecting ethanol-tolerant strains,especially for wines from increasingly warm climates.Lentilactobacillus hilgardii Q19 was isolated and characterized as an indigenous malolactic bacterium with higher ethanol tolerance properties.In this study,it was indicated that ethanol stress had significant effects on ATPase activity,antioxidant system,and cell membrane of L.hilgardii Q19 by measuring the physiological indicators under stress which include H^(+)-ATPase,Na^(+)/K^(+)-ATPase,Ca^(2+)/Mg^(2+)-ATPase activity,glutathione content,superoxide dismutase(SOD)activity and intracellular reactive oxygen species(ROS)content.The main metabolic pathways involved in ethanol stress such as ATP-binding cassette(ABC)transporters,pentose phosphate pathway,phosphotransferase system,glutathione metabolic pathway and two-component systems were screened by transcriptome sequencing analysis.The functions of the pentose phosphate pathway,pyruvate metabolic pathway and glycerolipid metabolism under ethanol stress were verified by constructing the L.hilgardii Q19 ethanol stress related key genes gnt K,pyk,and glp K overexpression vectors.The above findings may contribute to our understanding of the metabolic pathways and regulatory mechanisms of L.hilgardii Q19 in response to ethanol stress.展开更多
基金supported by the National Natural Science Foundation of China,Nos.32271043(to ZW)and 82171047(to YM)the both Science and Technology Major Project of Shanghai,No.2018SHZDZX01 and ZJLabShanghai Center for Brain Science and Brain-Inspired Technology(to ZW)。
文摘Downregulation of the inwardly rectifying potassium channel Kir4.1 is a key step for inducing retinal Müller cell activation and interaction with other glial cells,which is involved in retinal ganglion cell apoptosis in glaucoma.Modulation of Kir4.1 expression in Müller cells may therefore be a potential strategy for attenuating retinal ganglion cell damage in glaucoma.In this study,we identified seven predicted phosphorylation sites in Kir4.1 and constructed lentiviral expression systems expressing Kir4.1 mutated at each site to prevent phosphorylation.Following this,we treated Müller glial cells in vitro and in vivo with the m Glu R I agonist DHPG to induce Kir4.1 or Kir4.1 Tyr^(9)Asp overexpression.We found that both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited activation of Müller glial cells.Subsequently,we established a rat model of chronic ocular hypertension by injecting microbeads into the anterior chamber and overexpressed Kir4.1 or Kir4.1 Tyr^(9)Asp in the eye,and observed similar results in Müller cells in vivo as those seen in vitro.Both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited Müller cell activation,regulated the balance of Bax/Bcl-2,and reduced the m RNA and protein levels of pro-inflammatory factors,including interleukin-1βand tumor necrosis factor-α.Furthermore,we investigated the regulatory effects of Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression on the release of pro-inflammatory factors in a co-culture system of Müller glial cells and microglia.In this co-culture system,we observed elevated adenosine triphosphate concentrations in activated Müller cells,increased levels of translocator protein(a marker of microglial activation),and elevated interleukin-1βm RNA and protein levels in microglia induced by activated Müller cells.These changes could be reversed by Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression in Müller cells.Kir4.1 overexpression,but not Kir4.1 Tyr^(9)Asp overexpression,reduced the number of proliferative and migratory microglia induced by activated Müller cells.Collectively,these results suggest that the tyrosine residue at position nine in Kir4.1 may serve as a functional modulation site in the retina in an experimental model of glaucoma.Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression attenuated Müller cell activation,reduced ATP/P2X receptor–mediated interactions between glial cells,inhibited microglial activation,and decreased the synthesis and release of pro-inflammatory factors,consequently ameliorating retinal ganglion cell apoptosis in glaucoma.
文摘The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.595–603.
文摘The published article titled“Overexpression of miR-1283 Inhibits Cell Proliferation and Invasion of Glioma Cells by Targeting ATF4”has been retracted from Oncology Research,Vol.27,No.3,2019,pp.325–334.
文摘Correction to:Neurosci.Bull.December,2016,32(6):577–584.https://doi.org/10.1007/s12264-016-0068-z In this article,in Fig 5A,the picture of the Vector+Nogo-66 group was incorrect and should have appeared as shown below.
基金funded by Biological Breeding-National Science and Technology Major Project(2022ZD04008)Major Science and Technology Project of Hubei Province(2023BBA004)+1 种基金Jiujiang Municipal Key Research and Development Program(2025_001556)the China Agriculture Research System under the Ministry of Agriculture and Rural Affairs and the Chinese Academy of Agricultural Sciences,as well as the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI)。
文摘Drought and salt stresses are major abiotic factors that severely affect the growth,development,and yield formation of Brassica napus.Human derived FTO gene(Fat mass and obesity-associated),is a member of the Alk B family,encoding a m RNA demethylase that is dependent onα-ketoglutarate.Although previous studies have demonstrated that exogenous overexpression of FTO gene can increase plant biomass,its impact on plant stress resistance is still unclear.In this study,we cloned the FTO gene and conducted an analysis of its biological functions for drought and salt resistance for Brassicaceae plants.By overexpressing the FTO gene in Arabidopsis thaliana,the inhibitory effect of salt and drought stress on the root length growth of transgenic lines was significantly lower than that of the control.Moreover,the overexpression of FTO markedly enhanced the tolerance of Arabidopsis to drought and salt stress.It also led to a decrease in malondialdehyde(MDA)content,an increase in proline content,and a boost in superoxide dismutase(SOD)activity.Meanwhile,when the FTO gene was heterologously expressed in B.napus,the transgenic plants were less affected by stress.In comparison to control plants,they exhibited significantly lower MDA levels and markedly higher proline content and SOD activity.Furthermore,staining results with Trypan blue and nitroblue tetrazolium(NBT)staining indicate that the FTO gene can alleviate the damage to plants under stress and inhibit the accumulation of O_(2)^(-).Comprehensively,the results indicate that overexpression of the FTO gene can improve the drought and salt tolerance in transgenic plants,providing valuable references for further exploring the FTO-mediated stress resistance mechanisms.
基金supported by the National Key R&D Program of China(Grant no.2022YFC2807502)Qingdao Marine Science and Technology Center(Grant no.2022QNLM030003-1)Taishan Scholar Distinguished Expert Program in Shandong Province(Grant no.tstp20240504).
文摘A polyketide synthase-nonribosomal peptide synthetase gene cluster twn in Talaromyces sp.HDN1820200 was activated by overexpression of the pathway-specific transcriptional factor TwnD.Large-scale fermentation and chemical investigation of the mutant strain HDN1820200/TwnD led to the discovery of one new polyketide-amino acid conjugate,bipolamide C and one new polyketide compound,variotin A.The structures of the new compounds were determined by nuclear magnetic resonance(NMR)analysis,high-resolution electrospray ionization mass spectrometry,feeding experiments,NMR calculation and DP4^(+)analysis.This study revealed that the overexpression of the pathway-specific transcriptional factor represents a promising approach for the discovery of new natural products in fungi within specialized habitat.
基金financially supported by the National Natural Science Foundation of China(32160578)Natural Science Foundation of Ningxia Hui Autonomous Region(2025AAC020032).
文摘Malolactic fermentation,started by lactic acid bacteria,plays a crucial role in the production of high-quality wines.As global warming increases the ethanol content in wines,the success of malolactic fermentation depends on selecting ethanol-tolerant strains,especially for wines from increasingly warm climates.Lentilactobacillus hilgardii Q19 was isolated and characterized as an indigenous malolactic bacterium with higher ethanol tolerance properties.In this study,it was indicated that ethanol stress had significant effects on ATPase activity,antioxidant system,and cell membrane of L.hilgardii Q19 by measuring the physiological indicators under stress which include H^(+)-ATPase,Na^(+)/K^(+)-ATPase,Ca^(2+)/Mg^(2+)-ATPase activity,glutathione content,superoxide dismutase(SOD)activity and intracellular reactive oxygen species(ROS)content.The main metabolic pathways involved in ethanol stress such as ATP-binding cassette(ABC)transporters,pentose phosphate pathway,phosphotransferase system,glutathione metabolic pathway and two-component systems were screened by transcriptome sequencing analysis.The functions of the pentose phosphate pathway,pyruvate metabolic pathway and glycerolipid metabolism under ethanol stress were verified by constructing the L.hilgardii Q19 ethanol stress related key genes gnt K,pyk,and glp K overexpression vectors.The above findings may contribute to our understanding of the metabolic pathways and regulatory mechanisms of L.hilgardii Q19 in response to ethanol stress.
